Flavonoid biosynthesis facilitates low nitrogen adaptation in Gleditsia sinensis seedlings: Insights from comprehensive physiological and omics analyses.

Nitrogen (N) deficiency significantly constrains plant growth and the productivity of plantations. To elucidate the adaptation mechanisms of Gleditsia sinensis (G. sinensis) to low-N stress, an integrated analysis encompassing physiology, transcriptomics, and metabolomics was conducted on low-N tolerant (Changshun1, R) and sensitive (Luoting2, S) genotype seedlings.

Analysis of the AP2/ERF transcription factor family in Eriobotrya japonica and its role in exogenous melatonin-mediated regulation of salt stress.

AP2/ERF transcription factors (TFs) are involved in various regulatory pathways related to plant growth, development, and stress responses. The growth of Eriobotrya japonica, a popular fruit and medicinal plant, is affected by salt stress. Currently, the functions of E.

The SDF-1α/MTDH axis inhibits ferroptosis and promotes the formation of anti-VEGF-resistant choroidal neovascularization by facilitating the nuclear translocation of SREBP1.

Age-related macular degeneration (AMD) has been well recognized as the first ranked blinding ocular fundus diseases among older individuals, particularly in developed regions, owing to its progressive nature and high prevalence in aging populations. Anti-vascular endothelial growth factor (VEGF) agents injected into patients' vitreous cavity is the preferred treatment regimen for neovascular AMD. However, many patients exhibit resistance to anti-VEGF treatment, which is an urgent clinical problem.

Isolation effects and soil properties drive genetic differentiation in Ormosia microphylla populations.

The genetic characteristics of six wild populations of Ormosia microphylla were investigated and their soil properties were analyzed by using Hyper-seq sequencing technology. Based on the findings, targeted protection strategies are proposed. Genetic diversity analysis revealed moderate overall genetic diversity of O.

Genetic evolution of respiratory syncytial virus in pediatric acute respiratory infections: Insights from a non-epidemic season.

Background: Respiratory syncytial virus (RSV) is an important cause of acute respiratory infections (ARI) in children, with recent outbreaks increasing the global burden. The 2021 season saw a significant increase in cases from June to August, possibly due to COVID-19 control measures. The RSV-G region contributes to significant strain variation that can lead to severe epidemics.

Learning Self-Growth Maps for Fast and Accurate Imbalanced Streaming Data Clustering.

Streaming data clustering is a popular research topic in data mining and machine learning. Since streaming data is usually analyzed in data chunks, it is more susceptible to encountering the dynamic cluster imbalance issue. That is, the imbalance ratio (IR) of clusters changes over time, which can easily lead to fluctuations in either the accuracy or the efficiency of streaming data clustering.

CLK2-SOX3 combination promotes choroidal neovascularization by SGLT1 inducing endothelial cell metabolic reprogramming.

Choroidal neovascularization (CNV) is one of the main causes of visual loss. Endothelial cell metabolic reprogramming is an important mechanism in regulating pathological neovascularization. However, how endothelial cell metabolic reprogramming is regulated in CNV is not yet clear.

Functional characterization of the N assimilation pathways in the mycelium of Laccaria bicolor and the ectomycorrhizal symbiosis.

Ectomycorrhizal (ECM) fungi contribute to N, Pi, and water uptake in trees while obtaining carbohydrates from their host plants. However, the molecular mechanisms underlying N assimilation during ECM symbiosis remain unclear. In this study, we used RNA interference (RNAi) to silence the expression of genes encoding glutamine synthetase (GS) and NADP-glutamate dehydrogenase (GDH), which are key enzymes involved in N assimilation in the ectomycorrhizal basidiomycete, Laccaria bicolor.

Unravelling NK cell subset dynamics and specific gene signatures post-ibrutinib therapy in chronic lymphocytic leukaemia via single-cell transcriptomics.

Background: As part of the innate immune system, NK cells contribute to optimizing cancer immunotherapy strategies and are becoming a focal point in cancer research. However, limited research has been conducted to further investigate changes in NK cell subsets and their critical genes following ibrutinib treatment in CLL patients.

Methods: Peripheral blood samples from patients clinically and pathologically diagnosed with monoclonal B-cell lymphocytosis (MBL), newly diagnosed with CLL (ND-CLL), postibrutinib-treated patients who achieved a complete response (CR) or partial response (PR), and those with Richter's syndrome (RS) were collected.

High-fat diet alters retinal lipid composition and gene expression networks in mice.

Background: High-fat diet (HFD) was suggested to be associated with several retinal diseases, including age-related macular degeneration (AMD), glaucoma, and diabetic retinopathy (DR). Nevertheless, our understanding of the mechanisms governing retinal lipid metabolic homeostasis remains limited, with little attention focused on the influence of HFD on different retinal cell types. To address this gap, we established a high-fat model using mice fed with HFD for a duration of 6 months.

Correlation of computed fractional flow and in-stent restenosis in patients with intracranial atherosclerotic stenosis.

Objective: Fractional flow (FF) reserve has been developed as a gold standard for coronary intervention. Intracranial FF is also a valuable hemodynamic index to assess the severity of narrowing in intracranial atherosclerotic stenosis (ICAS). This study aims to investigate the predictive value of FF in assessing restenosis following endovascular treatment in patients with symptomatic ICAS.

How limited cognitive resources impact the attentional effects of self-talk: An eye-tracking study in dart.

The study aimed to investigate whether self-talk could enhance participants' motor performance and attention, even in the presence of distracting interferences and while experiencing ego depletion. To achieve this, 43 novices were randomly assigned into a self-talk group and a control group. Only the self-talk group received the self-talk intervention, and all participants performed a dart throwing task after experiencing ego depletion.

The role of vitamin K and its antagonist in the process of ferroptosis-damaged RPE-mediated CNV.

Age-related macular degeneration (AMD) is the leading cause of irreversible vision loss in people over the age of 55. AMD currently affects approximately 8% of the world's population, and the number is growing as the global population ages. Growing evidence suggests that pathological choroidal neovascularization (CNV) is often related to more severe and rapid vision loss and blindness associated with AMD.

Application of virtual deployment and hemodynamic simulation in treatment of a basal arterial dissecting aneurysm using flow diverter: A case report and literature review.

The application of flow diverters (FDs) to treat basilar artery (BA) aneurysms has been proven to be one of the most effective ways. The hemodynamic changes caused by FD have been proven to be a principal factor affecting the healing of aneurysms. Virtual deployment based on simulation technology can intuitively reflect the hemodynamic changes caused by FD, providing more information for the surgeon.

A new thiol-sulfur click chemistry for lithium-organosulfide batteries.

Click chemistry is a rapid, reliable, and powerful function and a highly selective organic reaction that facilitates the efficient synthesis of various molecules by joining small units. This approach has found widespread applications in fields such as drug development, chemical synthesis, and molecular biology. In recent years, the reaction of alkali-catalyzed polymerization of thiol and sulfur has been employed to prepare various sulfur-containing polymers, which are applied as electrochemical active electrode materials in the pursuit of good performance.

Glibenclamide targets MDH2 to relieve aging phenotypes through metabolism-regulated epigenetic modification.

Mitochondrial metabolism-regulated epigenetic modification is a driving force of aging and a promising target for therapeutic intervention. Mitochondrial malate dehydrogenase (MDH2), an enzyme in the TCA cycle, was identified as an anti-aging target through activity-based protein profiling in present study. The expression level of MDH2 was positively correlated with the cellular senescence in Mdh2 knockdown or overexpression fibroblasts.

Genetic association of MIR-449B, GCLC, eNOS, SORD, and ENPP1 with diabetic retinopathy.

Identifying the genetic risk factors of diabetic retinopathy (DR) is essential for discovering the potential pathogenesis of DR. This study determined the association of DR with five single nucleotide polymorphisms (SNPs) specifically in type 2 diabetes mellitus (T2DM) patients, including rs10061133(MIR-449B), rs17883901(GCLC), rs2070744(eNOS), rs3759890 (SORD) and rs7754561 (ENPP1). A total of 1433 individuals were enrolled in this study, comprising healthy controls (ctrls = 480), individuals with diabetes mellitus without retinopathy (DNR = 480), non-proliferative DR(NPDR = 378), and proliferative DR(PDR = 95).

Solution-Gated Thin Film Transistor Biosensor-Based SnO Amorphous Film for Label-Free Detection of Epithelial Cell Adhesion Molecules.

Epithelial cell adhesion molecule (EpCAM) was considered to be an important marker of multiple tumors, and its high expression is closely related to the early diagnosis and treatment of tumors. At present, metal oxide semiconductors have become a key component of biosensor and bioelectronics technology. Tin oxide shows great potential for development because of its nontoxic, nonpolluting, low price, and excellent electrical properties.

Effect of hybrid blood purification on nutritional status, inflammation, and cardiovascular events in patients with end-stage renal disease.

Objective: To explore the effects of hybrid blood purification on nutritional status and cardiovascular events in patients with end-stage renal disease (ESRD).

Methods: A total of 135 patients with ESRD who received treatment in The Affiliated Nanhua Hospital of Hengyang Medical School from March 2021 to June 2023 were included in this retrospective study. Of them, 66 patients were treated with hemodialysis purification (hemodialysis group), and 69 patients underwent hybrid blood purification (hybrid group).

Ginkgolide B binds to GPX4 and FSP1 to alleviate cerebral ischemia/reperfusion injury in rats.

Ischemia/reperfusion (I/R) injury can increase the anomalous permeability of the blood-brain barrier and the risk of hemorrhagic conversion. Ginkgolide B (Gin B) has been recognized for its neuroprotective properties in stroke treatment. This study aimed to analyze the association of Gin B with GPX4 and FSP1 in cerebral I/R injury treatment.

Screening and identification of evaluation indicators of low phosphorus tolerant germplasm in Gleditsia sinensis Lam.

This study aims to explore the low phosphorus (P) tolerance of saplings from different Gleditsia sinensis Lam. families. It also seeks to screen for Gleditsia sinensis families with strong low P tolerance and identify key indicators for evaluating their tolerance.

Mettl3-Mediated m6A Modification is Essential for Visual Function and Retinal Photoreceptor Survival.

Purpose: N6-methyladenosine (m6A) modification, one of the most common epigenetic modifications in eukaryotic mRNA, has been shown to play a role in the development and function of the mammalian nervous system by regulating the biological fate of mRNA. METTL3, the catalytically active component of the m6A methyltransferase complex, has been shown to be essential in development of in the retina. However, its role in the mature retina remains elusive.

aPKC/Par3/Par6 polarity complexes regulate podocyte motility and crescent formation in the progression of ANCA-associated vasculitis.

Objectives: Podocyte bridging may be a key initial event occurring early in crescent formation. This study aims to probe the underlying mechanism of atypical protein kinase C (aPKC)/protease-activated receptor 3(Par3)/Par6 polarity complexes on podocyte motility and crescent formation during the progression of antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV).

Methods: The effects of anti-TNF-α monoclonal antibody (mAb) on the crescent formation, localization and expression of aPKC/Par3/Par6 polarity complexes, and activities of small GTPases (Rho/Rac1/Cdc42) were explored in an AAV mouse model.

A comparative study on leaf anatomy and photosynthetic characteristics of different growth stages of .

The rare and endangered wild plant, , has been listed as a second-level key protected plant in China. Currently, its habitat is severely damaged, and the population has dramatically declined, necessitating urgent intervention for protection. In this study, the aim was to explore the correlations and differences from the perspectives of photosynthetic characteristics and leaf structure, providing scientific references for conservation and cultivation.

Living plant-assisted recycling of nano gold into Murray porous carbon electrode materials.

Based on the enrichment potential of living plants for nanoparticles, this paper develops a new strategy to utilize Murray's law in plants to remove various shapes of gold nanoparticles and, , convert them into Murray porous carbon. The inherent Murray network serves as an optimized hierarchical design and ensures the mechanical stability of the material, and Murray's law is employed to achieve uniform dispersion of nanoparticles , facilitating the preparation of metal nanoparticle-supported carbon materials.