High-quantum Yield Carbon Dots as Selective Fluorescence Turn-off Probe for Bromate Detection in Food Samples Via Catalytic Oxidation Mechanism.

In this study, nitrogen-doped carbon dots (N-CDs) were successfully synthesized through a facile one-step hydrothermal approach using honeysuckle and ethylenediamine (EDA) as carbon and nitrogen sources, respectively. The obtained N-CDs displayed intense blue fluorescence emission at 420 nm upon excitation at 350 nm, along with an exceptional fluorescence quantum yield of 60.53%.

Nanobody-based and aggregate quantum microspheres-integrated fluorescence immunochromatographic test strip for highly sensitive detection of aflatoxin B.

Aflatoxin B (AFB) is a highly toxic and potent carcinogenic mycotoxin that poses a significant threat to food safety. Conventional immunochromatographic test strips (ICTS) often exhibit insufficient specificity and sensitivity. To address these limitations, a novel fluorescent ICTS (AQMs@G8-ICTS) was developed by integrating aggregated quantum microspheres (AQMs) with the high-affinity nanobody G8.

Contribution of dietary methionine to gut health and its related diseases: implications for precision nutrition.

Gut health is intricately linked to energy homeostasis, stress resistance, inflammation, and longevity. Methionine (Met) intake significantly influences gut health, with both supplementation and restriction showing distinct effects. While appropriate Met supplementation offers benefits, excessive intake can be harmful, whereas Met restriction appears to improve overall health of the body, especially the gut.

Enhancing wheat bran quality: Comparing the effects of conventional thermal processing and microwave-assisted hydrolysis.

Wheat bran is a source of bioactive compounds at a low cost, but the cell wall matrix notably limits the accessibility of bran bioactive compounds. This study investigated the effect of hot air oven treatment (C-HAO), microwave treatment (C-MWO), and microwave-assisted hydrolysis (HY-MW) on the quality attributes of wheat bran (WB) and explored its digestibility during in vitro digestion. Conventional treatments yielded the highest omega-6/omega-3 ratio, while the HY-MW process at 70 °C demonstrated the lowest and the best ratio.

Dietary methionine restriction ameliorates atherosclerosis by remodeling the gut microbiota in apolipoprotein E-knockout mice.

Dietary methionine restriction (MR) has been shown to reduce the risk of atherosclerosis, but the specific regulatory effects and mechanisms remain unclear. This research intends to investigate the effects of MR on atherosclerosis in apolipoprotein E-knockout (ApoE) mice fed a high-fat, high-cholesterol, high-choline diet and their mechanisms. ApoE mice were fed a normal diet (0.

A portable fluorescent aptamer sensor for rapid quantitative detection of Hg.

Mercury(II) ions (Hg) are heavy metal ions that can cause human poisoning and can lead to death in severe cases. Therefore, establishing a rapid quantitative detection method for Hg is important for protecting human health. In this study, a portable hydrogel-based fluorescent aptasensor was developed for the rapid and quantitative detection of Hg.

Removal of Mycotoxins in Food by Emerging Porous Materials: Advances, Mechanisms and Prospects.

Mycotoxins are toxic secondary metabolites produced by various toxigenic fungi and are widely present in food and agricultural products, posing serious threats to food safety and human health. Effective removal of mycotoxin contamination from food remains a critical challenge in ensuring food security. Emerging porous materials (PMs) have recently gained significant attention in mycotoxin removal due to their unique properties.

Preparation of a 1,4-Diazabicyclo[2.2.2]octane sulfonate betaine zwitterionic stationary phase and comparative evaluation of its separation performance in hydrophilic interaction chromatography.

Background: Hydrophilic interaction liquid chromatography (HILIC) offers an alternative approach for efficient separation of polar molecules on polar stationary phases. The development of hydrophilic stationary phases with novel zwitterionic structures represents a promising approach to improving the efficiency of separations and accommodating the analysis of polar compounds in complex samples.

Results: This work details the preparation and thorough evaluation of the novel zwitterionic stationary phase, Sil-DBO-PS, composed of a 1,4-diazabicyclo[2.

Revealing the Characteristics and Correlations Among Microbial Communities, Functional Genes, and Vital Metabolites Through Metagenomics in Henan Mung Bean Sour.

Henan mung bean sour (HMBS) is the raw material for mung bean sour noodles (MBSNs), a traditional fermented food. To investigate the characteristic flavor compounds, we have detected the content of free amino acids (FAAs) and key metabolites including organic acids, sugars, and alcohols. The results revealed that the content associated with umami, sweetness, and bitterness (TVA > 1) showed significant differences.

Kinetic Fluorimetric Determination of Iridium Based on the Catalytic Oxidation of Doped Carbon Dots.

In this study, multi-element doped carbon dots (DXM-CDs) were synthesized via a one-step hydrothermal method, utilizing dexamethasone sodium phosphate (DXM) injection as a precursor. The synthesized DXM-CDs exhibited ultraviolet fluorescence emission at 380 nm, with a fluorescence quantum yield (QY) of 19.7%.

Research progress of photoelectrochemical sensors in food detection.

Food is the basic of the people, security is the basic of the food. As the quality of life improves, food safety has emerged as a global concern, making the development of simple, rapid, and efficient food safety detection methods critically important. Photoelectrochemical (PEC) sensors are a novel class of sensors developed in recent years that integrate photoelectric technology with biosensing.

Effective extraction and sensitive detection of aflatoxins in wheat by polymeric ionic liquid-based magnetic adsorbent with multiple interactions.

In this study, polymeric ionic liquids (PILs) modified magnetic nanoparticles were used to extract four aflatoxins (AFs) from wheat samples, providing a convenient and cost-effective sample preparation technique for AFs detection in the complex food matrix. The extraction parameters were optimized in terms of the amount of adsorbent, adsorption time, type and volume of desorption solvent, desorption time, and salt addition. The results showed that the proposed method had high sensitivity, good repeatability, and wide linear range.

Iodine crystallization-promoted efficient capture of iodine by nonporous covalent organic polymer.

The development of efficient iodine capture methods is essential for the safe utilization of nuclear energy. Here, a nonporous triazine-phenylenediamine covalent organic polymer (TA-PDA COP) with abundant nitrogen atoms and a π-conjugated system was used to capture iodine. The as-prepared adsorbent exhibited an iodine uptake of 5.

Structural characterization and antioxidant activity of a polysaccharide produced by CGMCC 6882 from waste tobacco leaves.

Due to the restricts of treatment methods and high costs, waste tobacco materials are usually discarded, which leads to resources waste and environmental pollution. Present work used waste tobacco leaves as carbon source to produce polysaccharide (CGP-TL) by CGMCC 6882 via submerged fermentation. CGP-TL yield was 1.

Effects of microbial fermentation on the anti-inflammatory activity of Chinese yam polysaccharides.

In this study, Chinese yam polysaccharides (CYPs) were fermented using M616, and changes in the chemical composition, structure, and anti-inflammatory activity of CYPs before and after fermentation were investigated. The carbohydrate content of M616-fermented CYP (CYP-LP) increased from 71.03% ± 2.

Applying hollow octahedron PtNPs/Pd-CuO nanozyme and highly conductive AuPtNPs/Ni-Co NCs to colorimetric -electrochemical dual-mode aptasensor for AFB1 detection.

Background: Aflatoxin B1 (AFB1) is a secondary metabolite produced by Aspergillus flavus and Aspergillus parasiticus. This toxin is highly carcinogenic and toxic, posing a serious threat to human and animal health. AFB1 primarily enters the human body through contaminated food, particularly peanuts, corn, nuts, and wheat.

Glutathione-functionalized covalent organic frameworks@silica as a hydrophilic-hydrophobic balanced mixed-mode stationary phase for highly efficient separation of compounds with a wide range of polarity.

Background: Covalent organic frameworks (COFs) are a highly promising stationary phase for high-performance liquid chromatography (HPLC), but the separation of polar compounds is limited by their low hydrophilicity. Therefore, it is crucial to develop novel COFs-based stationary phases with balanced hydrophilicity-hydrophobicity for the efficient separation of different polar compounds.

Results: In this paper, glutathione (GSH)-functionalized COFs@silica microspheres (GSH-COFs@SiO) were synthesized via a two-step, post-synthesis modification strategy.

Label-free ratiometric fluorescence detection of Pb structure-specific fluorescent dyes and dual signal amplification.

Lead ions (Pb) are a widely distributed and highly toxic heavy metal pollutant, which seriously threatens the environment, economy and human safety. Here, a label-free ratiometric fluorescent biosensor was constructed for Pb detection using DNAzyme-driven target cycling and exonuclease III (Exo III)-mediated DNA cycling as a dual signal amplification strategy. The SYBR Green I (SGI) and -methyl mesoporphyrin IX (NMM) used in this study are characterized by low cost, storage resistance, and short preparation time compared with conventional signaling probes labeled with fluorescent groups.

Triple-helix molecular-switch-actuated rolling circle amplification and catalytic hairpin assembly multistage signal amplified fluorescent aptasensor for detection of aflatoxin B1.

Background: Mycotoxins, a class of secondary metabolites produced by molds, are widely distributed in nature and are very common in food contamination. Aflatoxin B1 (AFB1) is a highly stable natural mycotoxin, and many agricultural products are easily contaminated by AFB1, it is important to establish a sensitive and efficient AFB1 detection method for food safety. The fluorescence aptamer sensor has shown satisfactory performance in AFB1 detection, but most of the fluorescence aptasensors are not sensitive enough, so improving the sensitivity of the aptasensor becomes the focus of this work.

Epicatechin and β-glucan from whole highland barley grain ameliorates hyperlipidemia associated with attenuating intestinal barrier dysfunction and modulating gut microbiota in high-fat-diet-fed mice.

Hyperlipidemia is associated with intestinal barrier dysfunction and gut microbiota dysbiosis. Here, we aimed at investigating whether epicatechin (EC) and β-glucan (BG) from whole highland barley grain alleviated hyperlipidemia associated with ameliorating intestinal barrier dysfunction and modulating gut microbiota dysbiosis in high-fat-diet-induced mice. It was observed that EC and BG significantly improved serum lipid disorders and up-regulated expression of PPARα protein and genes.

Procyanidin B1 and -coumaric acid from whole highland barley ameliorated HFD-induced impaired glucose tolerance small intestinal barrier and hepatic glucose metabolism.

Highland barley is a natural source for the development of phenolic compounds that exhibit potential in preventing type 2 diabetes, which is important for the agricultural and industrial utilization of highland barley. However, very few studies have focused on their effect on small intestinal absorption and barrier dysfunction, as well as the direct target for the modulation of hepatic glucose metabolism. In this study, procyanidin B1 (PB) and -coumaric acid (CA) isolated from highland barley supplementation in impaired glucose tolerance (IGT) mice significantly increased lactase-phlorizin hydrolase (LPH), sulfotransferase 1A1 (SULT1A1), UDP glucuronosyltransferase 1A (UGT1A) families and sodium-dependent glucose transporter 1 (SGLT1) expression in the small intestine of IGT mice, indicating beneficial effects on polyphenol deglycosylation and transportation.

Characterization, Mechanism, and Application of Dipeptidyl Peptidase III: An Aflatoxin B-Degrading Enzyme from HNGD-TM15.

Aflatoxin B is a notorious mycotoxin with mutagenicity and carcinogenicity, posing a serious hazard to human and animal health. In this study, an AFB-degrading dipeptidyl-peptidase III mining from HNGD-TM15 (ADPP III) with a molecular weight of 79 kDa was identified. ADPP III exhibited optimal activity toward AFB at 40 °C and pH 7.

Heat-moisture treatment of freshly harvested high-amylose maize kernels improves its starch thermal stability and enzymatic resistance.

The objective of this work was to study the effects of heat-moisture treatment (HMT) of freshly harvested mature high-amylose maize (HAM) kernels on its starch structure, properties, and digestibility. Freshly harvested HAM kernels were sealed in Pyrex glass bottles and treated at 80 °C, 100 °C, or 120 °C. HMT of HAM kernels had no impact on its starch X-ray diffraction pattern but increased the relative crystallinity.

Improvement of antibacterial activity of polysaccharides via chemical modification: A review.

Antibiotic residue and bacterial resistance induced by antibiotic abuse have seriously threatened food safety and human healthiness. Thus, the development and application of safe, high-efficiency, and environmentally friendly antibiotic alternatives are urgently necessary. Apart from antitumor, antivirus, anti-inflammatory, gut microbiota regulation, immunity improvement, and growth promotion activities, polysaccharides also have antibacterial activity, but such activity is relatively low, which cannot satisfy the requirements of food preservation, clinical sterilization, livestock feeding, and agricultural cultivation.