A ratiometric fluorescent aptamer assay based on RCA amplification mult-G-quadruplex.

Ratiometric assay with dual signals can effectively avoid false positives of single signals. Therefore, a dual-signal assay was designed to detect Aflatoxin B1 (AFB1) using the affinity of the aptamers to the target as well as the binding force between the DNA strands. The first fluorescent signal was obtained by binding AFB1 to aptamer to shed the complementary sequence with the FAM (6-carboxyfluorescein).

Genomic alterations in the YAP/TAZ pathway are associated with stem cell-like castration-resistant prostate cancer.

Unlabelled: Castration-resistant prostate cancer (CRPC) is an aggressive disease exhibiting multiple epigenomic subtypes: androgen receptor-dependent CRPC-AR, and lineage plastic subtypes CRPC-SCL (stem cell-like), CRPC-WNT (Wnt-dependent), and CRPC-NE (neuroendocrine). By transcriptomic profiling of tissue, and whole-genome sequencing (WGS) of tissue and cell-free DNA (cfDNA) from 500 samples, we relate genomic variants with epigenomic state. We find lineage plasticity is associated with higher epigenomic and genomic heterogeneity.

The Use of Capsaicin in the Treatment of Swallowing Disorders.

Swallowing disorders are a common clinical issue that significantly impacts patients' quality of life and nutritional intake. Recent years have seen a growing interest in capsaicin, a natural compound, due to its potential neuroprotective effects and its ability to stimulate the swallowing reflex. This review summarizes the research progress on the application of capsaicin in the treatment of swallowing disorders, detailing its mechanisms of action, clinical applications, and future research directions.

A weighted bag of visual words model for predicting fetal growth restriction at an early stage.

Purpose: Fetal growth restriction (FGR) is a significant concern for clinicians and pregnant women, as it is associated with increased fetal and neonatal mortality and morbidity. Although ultrasound has been the gold standard for many years to define FGR, it remains less than ideal for early detection of FGR. Placental dysfunction is a key factor in the development of FGR.

A portable fluorescent aptamer sensor for rapid quantitative detection of Hg.

Mercury(II) ions (Hg) are heavy metal ions that can cause human poisoning and can lead to death in severe cases. Therefore, establishing a rapid quantitative detection method for Hg is important for protecting human health. In this study, a portable hydrogel-based fluorescent aptasensor was developed for the rapid and quantitative detection of Hg.

Highly Accurate Occupational Pneumoconiosis Staging via Dark Channel Prior-Inspired Lesion Area Enhancement.

Occupational pneumoconiosis (OP) staging is the core for OP diagnosis. It is essentially an image classification task concerning patients' lung condition by analyzing their chest X-ray. To perform artificial intelligence-assisted OP staging, the chest X-ray film representational learning and classification are commonly adopted, where a convolutional neural network (CNN) has proven to be very efficient.

Loteprednol etabonate alleviates NLRP3 inflammasome-associated inflammatory diseases in mice by suppressing the transcription of IL-1β.

Excessive activation of the NLRP3 inflammasome leads to cellular inflammation and tissue damage. Finding an inhibitor of its activation is urgent need for NLRP3 inflammasome-associated inflammatory diseases. In this study, we identified Loteprednol etabonate (LE), a well-known anti-inflammatory drug for ocular conditions, as a potent inhibitor of NLRP3 inflammasome activation through screening an FDA-approved drug library.

Discussion on the mechanism of HER2 resistance in esophagogastric junction and gastric cancer in the era of immunotherapy.

Human epidermal growth factor receptor 2 (HER2) is a critical biomarker and therapeutic target in gastric/gastroesophageal junction (G/GEJ) cancers, despite the initial success of HER2-targeted therapies, such as trastuzumab, resistance to these drugs has emerged as a major impediment to effective long-term treatment. This review examines the mechanisms of drug resistance in HER2-positive G/GEJ cancer, the primary mechanisms of resistance explored include alterations in the HER2 receptor itself, such as mutations and changes in expression levels, as well as downstream signaling pathways, and interactions with the tumor microenvironment (TME). Furthermore, the review discusses the Novel therapeutic approaches, including the use of antibody-drug conjugates (ADCs) and combination therapies are assessed for their potential to enhance outcomes.

Clinical Values of Serum Platelet-Activating Factor in Hypertensive Disorders Complicating Pregnancy.

Introduction: Serum platelet-activating factor (PAF) was proven to be associated with gestational hypertension. However, the predictive value of serum PAF at early pregnancy for the occurrence and outcomes of hypertensive disorders complicating pregnancy (HDCP) remained unclear.

Methods: The demographic and clinical characteristics of patients were compared among the different subgroups.

Effects of magnesium sulfate combined with labetalol on vascular endothelial function and pregnancy outcomes in pregnant women with preeclampsia.

Objective: To investigate the effects of magnesium sulfate combined with labetalol treatment on vascular endothelial function and pregnancy outcomes in pregnant women with preeclampsia (PE).

Methods: In this retrospective study, clinical data was analyzed from 262 PE parturients who were treated in Xi'an High-Tech Hospital from January 2022 to February 2024. They were divided into the experimental group (138 cases) and the control group (124 cases) based on the treatment plan.

SARS-CoV-2 Nsp7 plays a role in cognitive dysfunction by impairing synaptic plasticity.

It has been reported that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection can result in long-term neurological symptoms such as cognitive dysfunction, however the specific mechanisms underlying this phenomenon remain unclear. Initially, we confirmed a reduction in the level of synaptic proteins in SH-SY5Y neurons following SARS-CoV-2 infection. SARS-CoV-2 Nsps are crucial for the efficient replication of the virus and play important roles in the interaction between virus and host cell.

Accurate pneumoconiosis staging via deep texture encoding and discriminative representation learning.

Accurate pneumoconiosis staging is key to early intervention and treatment planning for pneumoconiosis patients. The staging process relies on assessing the profusion level of small opacities, which are dispersed throughout the entire lung field and manifest as fine textures. While conventional convolutional neural networks (CNNs) have achieved significant success in tasks such as image classification and object recognition, they are less effective for classifying fine-grained medical images due to the need for global, orderless feature representation.

A systematic review of gastritis as an immune-related adverse event in clinical interventions.

Immune checkpoint inhibitors (ICIs) are crucial in cancer treatment, and the associated immune-related adverse events (irAEs) have garnered significant attention, yet reports on associated immune related gastritis are limited. The diagnosis of immune related gastritis remains predominantly exclusionary, meanwhile its management diverges significantly from that of conventional gastritis. Current guidelines lack standardized grading criteria, and substantial data from large-scale, tertiary clinical studies are absent, therefore we conducted a systematic review of Medline, Web of Science, and Embase databases, identifying 31 articles from 2017 to December 31, 2023, involving 258 patients.

Texture-Based Classification of Fetal Growth Restriction From Intrauterine Neurosonographic Image.

Objective: Fetal growth restriction (FGR) is a condition where fetuses fail to reach their genetic potential for growth, posing a significant health challenge for newborns. The aim of this research was to explore the efficacy of texture-based analysis of neurosonographic images in identifying FGR in fetuses, which may provide a promising tool for early assessment of FGR.

Methods: A retrospective analysis collected 100 intrauterine neurosonographic images from 50 FGR and 50 gestational age-appropriate fetuses.

Predictive value of near-term prediction models for severe immune-related adverse events in malignant tumor PD-1 inhibitor therapy.

Immune-related adverse events (irAEs) impact outcomes, with most research focusing on early prediction (baseline data), rather than near-term prediction (one cycle before the occurrence of irAEs and the current cycle). We aimed to explore the near-term predictive value of neutrophil/lymphocyte ratio (NLR), platelet/lymphocyte ratio (PLR), absolute eosinophil count (AEC) for severe irAEs induced by PD-1 inhibitors. Data were collected from tumor patients treated with PD-1 inhibitors.

Oncological characteristics, treatments and prognostic outcomes in MMR-deficient colorectal cancer.

Colorectal cancer (CRC) ranks as the third most prevalent cancer globally. It's recognized that the molecular subtype of CRC, characterized by mismatch repair deficiency (dMMR) or microsatellite instability-high (MSI-H), plays a critical role in determining appropriate treatment strategies. This review examines the current molecular classifications, focusing on dMMR/MSI-H CRC and its subtypes: Lynch syndrome (LS), Lynch-like syndrome (LLS), and sporadic cases.

ViPRA-Haplo: De Novo Reconstruction of Viral Populations Using Paired End Sequencing Data.

We present ViPRA-Haplo, a de novo strain-specific assembly workflow for reconstructing viral haplotypes in a viral population from paired-end next generation sequencing (NGS) data. The proposed Viral Path Reconstruction Algorithm (ViPRA) generates a subset of paths from a De Bruijn graph of reads using the pairing information of reads. The paths generated by ViPRA are an over-estimation of the true contigs.

The association between prepregnancy dietary fatty acids and risk of gestational diabetes mellitus: A prospective cohort study.

Background & Aims: Epidemiologic studies have examined the association between dietary fatty acids and type 2 diabetes risk in general populations. Evidence regarding their associations with gestational diabetes mellitus (GDM) risk remains limited. This study aimed to evaluate prepregnancy fatty acids intake in relation to GDM risk.

Correction: Sun et al. Ergosterol Isolated from Suppresses LPS-Induced Neuroinflammatory Responses in Microglia Cells and ICR Mice. 2023, , 2406.

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A Generalized Nesterov-Accelerated Second-Order Latent Factor Model for High-Dimensional and Incomplete Data.

High-dimensional and incomplete (HDI) data are frequently encountered in big date-related applications for describing restricted observed interactions among large node sets. How to perform accurate and efficient representation learning on such HDI data is a hot yet thorny issue. A latent factor (LF) model has proven to be efficient in addressing it.

Anti-PD-1 immunotherapy combined with stereotactic body radiation therapy and GM-CSF for the treatment of advanced malignant PEComa: A case report.

Background: Perivascular epithelioid cell neoplasm (PEComa) is a rare mesenchymal tumour. Due to its low incidence, a standard treatment regimen for PEComa has not yet been established. Radiotherapy has a synergistic effect with PD-1 inhibitors and GM-CSF.