POU6F1 promote lumbar motor circuit reorganization following spinal cord injury.

Spinal cord injury (SCI) causes irreversible motor deficits due to disrupted lumbar circuitry. However, transcriptional mechanisms in distal lumbar circuits are poorly understood. We identify POU6F1 as a critical transcriptional regulator in spinal lumbar segment (SLS, L3-L5) motor circuit regeneration.

Associations of Rare Variants in the Gene with Bipolar Disorder in Chinese Population.

Purpose: This pioneering study aimed to explore the associations between the A-kinase anchoring protein 11 () gene and bipolar disorder (BD) in a Chinese population. We sought to replicate findings from European populations regarding ultra-rare protein-truncating variants (PTVs) within exon 8 of and identify any novel rare mutations linked to Chinese BD patients.

Methods: We conducted a case-control association study, including a cohort of 284 Chinese BD patients, with the control group comprising 10,588 individuals from the China Metabolic Analytics Project (ChinaMAP) database.

Research of text paraphrase generation based on self-contrastive learning.

The goal of this study is to improve the quality and diversity of text paraphrase generation, a critical task in Natural Language Generation (NLG) that requires producing semantically equivalent sentences with varied structures and expressions. Existing approaches often fail to generate paraphrases that are both high-quality and diverse, limiting their applicability in tasks such as machine translation, dialogue systems, and automated content rewriting. To address this gap, we introduce two self-contrastive learning models designed to enhance paraphrase generation: the Contrastive Generative Adversarial Network (ContraGAN) for supervised learning and the Contrastive Model with Metrics (ContraMetrics) for unsupervised learning.

Isorhynchophylline Mitigates Bone Loss in OVX Mice by Modulating Inflammatory Responses, Oxidative Stress, Gut Microbiota Composition and SCFAs Production.

Osteoporosis (OP) is a systemic skeletal disorder characterized by reduced bone mass and deteriorated bone architecture. Isorhynchophylline (IRN), an active alkaloid derived from the plant , is known for its significant anti-inflammatory and antioxidant properties. However, its potential role in mitigating bone loss has not been investigated.

Identification of In Vivo Components in Shiduqing Capsule Using High-Resolution Mass Spectrometry and Network Pharmacology-Based Analysis of Its Therapeutic Mechanisms Against Eczema.

Rationale: Shiduqing Capsule, a clinically widely used Chinese patent medicine, is indicated for skin itching, dryness, and desquamation caused by blood deficiency with wind-dryness syndrome. However, the tissue distribution characteristics of its active components in vivo and its pharmacological mechanisms in treating eczema remain unclear. This study aims to identify the bioactive components of Shiduqing Capsule, characterize their in vivo tissue distribution patterns, and subsequently elucidate the underlying therapeutic mechanisms against eczema based on these findings.

Design principles for social-ecological research at the landscape scale applied to western Rwanda.

Place-based social-ecological systems research provides major opportunities to advance sustainability and often involves large, interdisciplinary groups. Researchers adopt various methodologies when studying landscapes, gathering a wide array of data such as socioeconomic information from households, ecological data from specific areas, and qualitative insights from interviews. To integrate these varied methods, we propose identifying social-ecological research units as shared anchor points for data collection across teams.

Nitro-oleic acid activation of endothelial PPARγ signaling pathway alleviates neurovascular injury and improves functional outcomes in ischemic stroke.

Nitro-oleic acid (OA-NO) is an endogenous peroxisome proliferator-activated receptor-γ (PPARγ) ligand and can activate this receptor under both physiological and pathological conditions. In this study, we explore the role and molecular mechanisms of OA-NO in maintaining blood-brain barrier (BBB) integrity and enhancing neurovascular function during ischemic stroke, with a particular emphasis on the activation of endothelial PPARγ signaling pathways. Endothelial cell-selective PPARγ conditional knockout (EC-PPARγ cKO) and wild-type (WT) mice underwent 1 h middle cerebral artery occlusion (MCAO) with 1-7 days of reperfusion.

Discovery of 7-azaindole-3-acrylamide inhibitors of inflammasomes/IL-1β for the treatment of inflammatory bowel disease.

Currently, a significant proportion of patients with inflammatory bowel disease (IBD) fail to respond to conventional drug therapies such as immunosuppressants and biologic agents. IL-1 signaling blockade is a promising therapeutic strategy for these unresponsive IBD patients. In this study, we identified a novel anti-NLRP3/ IL-1β inhibitor, the 7-azaindole analogue Y19, which exhibits an IC value of 1.

A multicenter randomized controlled trial of intrapleural perfusion of methotrexate-loaded tumor cell-derived microparticles combined with systemic therapy for malignant pleural effusion.

This study evaluated the efficacy and safety of intrapleural perfusion with methotrexate-loaded tumor cell-derived microparticles (MTX-TMPs) combined with systemic therapy (ST) in patients with malignant pleural effusion (MPE) secondary to lung or breast cancer. In this multicenter, randomized, open-label trial, 102 patients were assigned 1:1 to receive either MTX-TMPs intrapleural perfusion (50 mL daily for 4 days) plus ST (cohort 1) or interleukin-2 (IL-2) intrapleural perfusion (50 mL every 3 days for three sessions) plus ST (cohort 2). The objective response rate (ORR) and disease control rate (DCR) of pleural effusion were evaluated in 91 patients (50 in cohort 1, 41 in cohort 2).

Ovarian cancer after breast cancer in women with a BRCA1 or BRCA2 pathogenic variant.

Objective: Inherited BRCA1/2 pathogenic variants (mutations) confer high lifetime risks of breast and ovarian cancers. Estimating the cumulative ovarian cancer risk following a breast cancer diagnosis in these women will help guide decisions regarding preventive salpingo-oophorectomy.

Methods: Women carrying a BRCA1 or BRCA2 mutation were followed after breast cancer and completed follow-up questionnaires every two years.

Discovery of novel 8-hydroxyquinoline derivatives as NLRP3 inflammasome inhibitors with therapeutic potential for inflammatory bowel disease.

The activation of the NLRP3 inflammasome occurs through two distinct phases-priming and activation-and plays a significant role in various diseases including inflammatory conditions, cancer, and autoimmune disorders. Recent studies have highlighted its critical involvement in the development and progression of inflammatory bowel disease (IBD). In this study, we identified a novel 8-hydroxyquinoline derivative, compound 10, as a potent inhibitor of NLRP3 inflammasome activation.

MdPOB1L Positively Regulates Disease Resistance Against Botryosphaeria dothidea by Manipulating MdNPR1 Protein Stability in Malus domestica.

E3 ubiquitin ligase is a key component of the ubiquitin-proteasome system, which is deeply involved in multiple aspects of plant growth and development, including in plant defence responses. POZ/BTB containing-protein1 (POB1) is a type of BTB-BACK domain-containing E3 ligase, which was previously reported to be a negative regulator of defence responses in multiple plant species. In this report, we identified MdPOB1-like (MdPOB1L) as a positive regulator in defence responses against Botryosphaeria dothidea by manipulating protein stability of MdNPR1, a master regulator in salicylic acid (SA) signalling pathway, in apple (Malus domestica).

Real-world single-center data analysis of dual immunotherapy in advanced NSCLC: Efficacy, survival, and adverse events.

We collected 96 patients who received dual immunotherapy between January 2019 and June 2024, in a 1:1:1 ratio based on PD-L1 expression levels of negative (<1%), medium (1% ≤ PD-L1 ≤ 49%), and high (PD-L1 ≥ 50%). This study evaluates the efficacy and safety of dual immunotherapy in patients with advanced NSCLC, stratified by PD-L1 expression levels. Among the 96 patients, the objective response rate (ORR) was 37.

Breaking the toxicity-efficacy tradeoff: Discovery of isoxazoline derivative as candidate for green insecticide.

Commercially available insecticides generally suffer from high toxicity to bees. The inherent toxicity-efficacy trade-off in traditional insecticides has become a major bottleneck constraining the advancement of green pesticide innovation. In this study, a series of novel triazole amide isoxazoline derivatives were designed and synthesized using DP-9 as the lead compound.

Cultivating Fully Biomass Pressure Sensors for Gait Analysis.

Incorporating biomass (e.g., bacterial cellulose, BC) pressure-sensing structures into plantar monitoring technologies could effectively defend users against insecure wear and inaccurate detection, while their capability in sensing pressure continuously when subjected to large pressure is hindered by their natural two-dimensional structure.

Epigenetic factors associated with peri-implantitis: a review.

Peri-implant diseases are characterized by the resorption of hard tissue and the inflammation of soft tissue. Epigenetics refers to alterations in the expression of genes that are not encoded in the DNA sequence, influencing diverse physiological activities, including immune response, inflammation, and bone metabolism. Epigenetic modifications can lead to tissue-specific gene expression variations among individuals and may initiate or exacerbate inflammation and disease predisposition.

Prevalence and predictive importance for short-term clinical outcomes of GLIM-defined malnutrition among adult inpatients: A nationwide cross-sectional study.

Background & Aims: Malnutrition is a significant health issue in hospital settings, with persistent diagnostic challenges. Despite the Global Leadership Initiative on Malnutrition (GLIM) criteria providing a standardised approach, nationwide validation and implementation remain limited. We aimed to estimate the national prevalence of GLIM-defined malnutrition among Chinese adult inpatients and assess the predictive value of the GLIM criteria for short-term clinical outcomes.

Trends and emerging frontiers of photodynamic therapy for non-melanoma skin cancer (1979-October 2024): a bibliometric analysis.

Introduction: Photodynamic therapy (PDT) is a non-invasive treatment modality for non-melanoma skin cancer (NMSC). This study aims to use bibliometric analysis to explore the research trends and development in the field of PDT for NMSC, identifying key hotspots and emerging directions.

Methods: This study conducted a comprehensive literature search in the Web of Science Core Collection database and applied bibliometric analysis and visualization using tools such as VOSviewer, CiteSpace, and the R package "bibliometrix".

The Mechanism of Micrografting With Salt-Tolerant Rootstock in Improving the Salt Tolerance of Scion in Vitis vinifera.

Grapevine (Vitis vinifera L.) is highly sensitive to soil salinization, which severely restricts its cultivation in salt-affected areas. In this study, "Pinot Noir" (V.

Ethanol extract of seeds alleviates HGHFD/STZ-induced nonalcoholic fatty liver disease in diabetic rats by modulating oxidative stress, inflammation, and lipid accumulation.

Objectives: To investigate the ameliorative effects of ethanol extract (EGK) on type 2 diabetes mellitus (T2DM) combined with nonalcoholic fatty liver disease (NAFLD) and to explore its underlying mechanisms.

Materials And Methods: , a T2DM rat model was established using HGHFD/STZ. , HepG2 cells were induced with FFA to create a model of lipid accumulation.

Bioactive expression of eukaryotic cytochrome P450 ferulate-5-hydroxylase in Escherichia coli for sustainable synthesis of antioxidant 5-hydroxyferulic acid.

The eukaryotic cytochrome P450 ferulate-5-hydroxylase (F5H), a membrane-bound protein, plays a critical role in lignin synthesis involved in the biosynthesis of 5-Hydroxyferulic acid (5-HFA) from ferulic acid, with 5-HFA offering enhanced antioxidant properties. However, there is challenging to engineer recombinants for 5-HFA synthesis by expressing F5H in Escherichia coli. In this work, we co-expressed F5H derived from Arabidopsis thaliana (AtF5H) and NADPH-dependent cytochrome P450 reductase (CPR) in E.

Comparison of efficacy and safety of a proposed biosimilar QL1206 with reference denosumab in patients with bone metastasis from breast cancer: A subgroup analysis of a randomized, double-blinded phase III study.

Objective: To evaluate the efficacy and safety of QL1206 (a denosumab biosimilar to Xgeva®) in breast cancer patients with bone metastasis (BM) through subgroup analysis of a randomized, double-blind phase III trial (No. NCT04550949).

Methods: This subgroup analysis included patients with BM from breast cancer enrolled in a phase III trial.

Stillbirth increase long-term health risks of diabetes, CVD, CVD mortality, and all-cause mortality.

Background: Fewer studies on association of single stillbirth exposure with long-term health outcomes in women. In this study of cohort data from UK Biobank, we aim to explore the association of stillbirth with the risk of diabetes, cardiovascular disease (CVD), all-cause mortality, CVD mortality, ischemic heart disease (IHD), myocardial infarction (MI), heart failure (HF), and stroke.

Methods: We initially included 502,366 participants aged 40-69 years.

Resveratrol ameliorates early retinal neurodegeneration in diabetic retinopathy via microRNA-29b/specificity protein 1/apoptosis pathway by enhancing autophagy.

Purpose: The aim of this study was to investigate the improved effect of resveratrol (RSV) on early retinal neurodegeneration in diabetic retinopathy (DR) via regulating autophagy/ microRNA-29b (miR-29b)/ specificity protein 1 (SP1)/ apoptosis pathway.

Methods: Retinal tissues from normal rats and diabetic rats after 12 weeks of intervention, and retinal Müller cells (RMCs) from normal and high glucose (HG) cultures, with or without rapamycin (RAPA), 3-methyladenine (3-MA), and RSV treatments, were used. Retinal neurofunctional changes were measured by electroretinogram.