Complete genome sequence of SJ-1, a marine bacterium capable of degrading -chloro--xylenol.

We report the complete genome sequence of SJ-1, the first marine-derived bacterial strain capable of degrading the antibacterial agent -chloro--xylenol. The genome consists of one chromosome (5,438,782 bp) and three plasmids (197,992 bp, 59,967 bp, and 41,343 bp), with an average GC content of 70.4%.

Deciphering the enzymatic responses, composition, network complexity, and functional degraders of the marine sediment bacterial community in response to 2-methyl-4-isothiazoline-3-one exposure.

2-Methyl-4-isothiazoline-3-one (MIT) is a widely employed antimicrobial agent frequently detected in environments; however, current documentation regarding its impacts on microbial communities and biodegradation processes remains limited. Herein, marine sediment systems were established to investigate the MIT impacts under tiered concentrations (CK, 0 μg/L; LC, 5 μg/L; MC, 50 μg/L; HC 5 mg/L). The extracellular polymeric substance contents increased in the LC and MC groups, but not in the HC group, after one day of exposure.

Elucidating the metabolic pathway and initial degradation gene for -chloro--xylenol biodegradation in DMU114.

Unlabelled: The antimicrobial agent -chloro--xylenol (PCMX), an emerging environmental pollutant, poses ecological risks; however, its biodegradation mechanisms remain unresolved. Here, we elucidate the metabolic pathway and functional genes involved in the initial catabolic step of PCMX in a newly isolated bacterium, DMU114. Pure-culture and synthetic consortium assays confirmed the pivotal role of in PCMX degradation, despite its relatively low abundance in the PCMX-enriched consortium.

Sowing date optimization mitigates cadmium accumulation in rice-ratoon rice cropping systems.

Background: Cadmium (Cd) contamination in rice has become a global issue. However, limited information exists regarding the impact of sowing dates on Cd accumulation in ratoon rice (RR) and its associated health risks. This study involved a two-season field experiment on rice-ratoon rice, which was conducted on a mildly Cd-contaminated soil in southern China.

Molecular mechanism of drug inhibition of URAT1.

Hyperuricemia, characterized by elevated serum urate levels, is a key factor in the pathogenesis of gout. URAT1 is essential for renal urate reabsorption and has emerged as a critical therapeutic target for managing hyperuricemia. However, the precise transport mechanism and the inhibitory effects of uricosuric drugs on URAT1 remain unclear.

Deciphering the intricate microbial responses and potential degraders of p-chloro-m-xylenol in marine sediments.

The widespread use of p-chloro-m-xylenol (PCMX) as a broad-spectrum antimicrobial agent raises concerns about its ecological risks in ecosystems. While prior studies focused on activated sludge systems, the impacts of PCMX on marine systems remain unknown. Here, we systematically investigated the responses of marine sediment communities to PCMX (0.

Synthesis of isoluminol derivatives with a terminal carboxyl group for protein labelling.

-(4-Aminobutyl)--ethylisoluminol (ABEI) as an efficient label is widely used in automated immunoassays, yet the labelling process of ABEI onto biomacromolecules like proteins is not straightforward as the transformation of an amino group into a carboxyl group is needed. We report the synthesis of isoluminol derivatives with a terminal carboxyl group for protein labelling. And also, an -hydroxysuccinimide active ester of carboxylic acid as a direct protein labelling reagent was isolated with high purity.

Strategic attenuation of Cd accumulation in rice through stage-specific flooding: Synergistic coordination of rhizospheric Cd bioavailability, microbial communities, and iron plaque speciation.

Continuous flooding (CF) effectively mitigates rice cadmium (Cd) contamination but raises concerns about water scarcity and greenhouse gas emissions, limiting sustainability. Therefore, there is a need for water management strategies that can reduce Cd accumulation in rice without the substantial resource demands of CF. Field trials conducted in Cd-contaminated paddy fields in southern China compared eight water management strategies, including continuous drainage (CD), stage-specific flooding, and CF, to identify alternatives for reducing rice Cd accumulation.

Demand analysis of transitional care for patients undergoing minimally invasive cardiac interventions with AI-driven solutions: a mixed-methods approach.

Aims: Minimally invasive cardiac intervention (MICI) patients remain at high risk of readmission and mortality during their post-discharge phase, with 30-day readmission rates of up to 10%. Although technological innovations, especially AI-driven solutions, hold promise for improving outcomes, there is a pressing need to clarify the full spectrum of patient demands during the transition from hospital to home. This study aimed to systematically identify these demands to guide the development of AI-driven solutions that reduce readmission rates and improve clinical outcomes.

AutoCOPD-A novel and practical machine learning model for COPD detection using whole-lung inspiratory quantitative CT measurements: a retrospective, multicenter study.

Background: The rate of diagnosis for chronic obstructive pulmonary disease (COPD) is low worldwide. Quantitative computed tomography (QCT) parameters add value to quantify alterations in airway and lung parenchyma for COPD. This study aimed to assess the performance of QCT features in COPD detection using a whole-lung inspiratory CT model.

Parameter-sensitive life cycle assessment of sludge incineration technologies integrating energy balance model.

Incineration is vital for safe sewage sludge treatment and resource recovery in China, using methods like mono-incineration (INC), and co-incineration in coal plant (CINP), cement kiln (CINC), and municipal solid waste incineration plant (CINM). Existing studies suffer from poor inventory quality and inaccurate quantification. To overcome these challenges, this study integrates the energy balance model with life cycle assessment to evaluate key system parameters, quantify co-incineration disturbances, and assess environmental impacts.

Deciphering the treatment performance, microbial community responses, and behavior of antibiotic resistance genes in anaerobic sequencing batch reactors under graphene exposure.

Graphene has garnered significant attention due to its unique and remarkable properties. The widespread application of graphene materials in numerous fields inevitably leads to their release into the environment. This study examines the long-term impacts of graphene on anaerobic sequencing batch reactors.

Binding mechanism and antagonism of the vesicular acetylcholine transporter VAChT.

The vesicular acetylcholine transporter (VAChT) has a pivotal role in packaging and transporting acetylcholine for exocytotic release, serving as a vital component of cholinergic neurotransmission. Dysregulation of its function can result in neurological disorders. It also serves as a target for developing radiotracers to quantify cholinergic neuron deficits in neurodegenerative conditions.

Responses of microbial communities in coastal sediments exposed to triclocarban and triclosan.

Triclocarban (TCC) and triclosan (TCS) are applied in a wide range of pharmaceutical and personal care products to prevent or reduce bacterial growth. Due to their extensive application, they are frequently detected in marine environments. In this study, marine sediment systems exposed to different concentrations of TCC and TCS were established to evaluate their effects on microbial communities.

Indigenized Characterization Factors for Health Damage Due to Ambient PM in Life Cycle Impact Assessment in China.

Life cycle assessment (LCA) is a broadly used method for quantifying environmental impacts, and life cycle impact assessment (LCIA) is an important step as well as a major source of uncertainties in LCA. Characterization factors (CFs) are pivotal elements in LCIA models. In China, the health loss due to ambient PM is an important aspect of LCIA results, which, however, is generally assessed by adopting CFs developed by global models and there remains a need to integrate localized considerations and the latest information for more precise applications in China.

Impacts of PFOS, PFOA and their alternatives on the gut, intestinal barriers and gut-organ axis.

With the restricted use of perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA), a number of alternatives to PFOS and PFOA have attracted great interest. Most of the alternatives are still characterized by persistence, bioaccumulation, and a variety of toxicity. Due to the production and use of these substances, they can be detected in the atmosphere, soil and water body.

The impact on Cd bioavailability and accumulation in rice (Oryza sativa L.) induced by dry direct-seeding cultivation method in field-scale experiments.

Dry direct-seeded rice cultivation has gained popularity and expanded its cultivated area due to reduced labor requirements and water consumption. However, the impact of this cultivation method on cadmium (Cd) bioavailability in soil and the accumulation levels in grains remains uncertain. Field experiments were conducted in acidic soils at two locations in southern China to compare rice varieties and evaluate the dry direct-seeding method alongside the wet direct-seeding and traditional transplanting methods.

Co-culture of benzalkonium chloride promotes the biofilm formation and decreases the antibiotic susceptibility of a strain.

Benzalkonium chloride (BAC) is a disinfectant with broad-spectrum antibacterial properties, yet despite its widespread use and detection in the environment, the effects of BAC exposure on microorganisms remain poorly documented. Herein, the impacts of BAC on a strain Jade-X were systematically investigated. The results demonstrated that the minimum inhibitory concentration (MIC) of BAC against strain Jade-X was 64 mg L.

A deep insight into ferroptosis in lung disease: facts and perspectives.

In the last decade, ferroptosis has received much attention from the scientific research community. It differs from other modes of cell death at the morphological, biochemical, and genetic levels. Ferroptosis is mainly characterized by non-apoptotic iron-dependent cell death caused by iron-dependent lipid peroxide excess and is accompanied by abnormal iron metabolism and oxidative stress.

Biodegradation of chloroxylenol by an aerobic enrichment consortium and a newly identified Rhodococcus strain.

Chloroxylenol is a commonly used antimicrobial agent in antibacterial and disinfection products, which has been detected in various environments, such as wastewater treatment plants, rivers, seawater, and even drinking water, with concentrations ranging from ng/L to mg/L. However, the biodegradation of chloroxylenol received limited attention with only sporadic reports available so far. In this study, an efficient chloroxylenol-degrading consortium, which could degrade 20 mg/L chloroxylenol within two days, was obtained after five months of enrichment.

Unraveling the signaling roles of indole in an opportunistic pathogen Pseudomonas aeruginosa strain Jade-X.

Pseudomonas aeruginosa, which can produce several toxins and form biofilm, is listed among the priority pathogens. Indole is a ubiquitous aromatic pollutant and signaling molecule produced by tryptophanase in bacteria. Herein, the impacts of indole on a newly isolated P.

Multicity comparative assessment and optimized management path of sustainability of the economy-energy-environment system: A case study of core cities in China's three major economic circles.

Coordinated and stable development of economy-energy-environment (3E) systems represents a long-term strategy for the sustainable development of humankind. Following the research idea of "indicator system construction-3E system evaluation-obstacles identification-optimization management," this article innovatively constructs a multiangle and comparable methodology system for evaluation and optimized management of the 3E system and considers the core cities of three economic circles in China as cases for empirical research. The results show that all the coordination degree levels were of good or high quality, which was at the highest level in the country.

Sulfate radical-based advanced oxidation process effects on tire wear particles aging and ecotoxicity.

Tire wear particles (TWPs) are widely distributed in natural water and pose as major pollutants in aquatic environments. In this study, heat-activated persulfate (HPT) and ultraviolet-activated persulfate treatments (UPT) were employed to investigate the influence of sulfate radical (SO•)-based advanced oxidation process (SAOPs) on TWP physicochemical properties and to clarify their ecotoxic effects in laboratory-level studies. Results showed that the specific surface areas of TWPs increased after UPT but decreased after HPT.

Is solar heating an appropriate choice in rural areas of northern China? Evidence from numerical simulation and life cycle assessment.

Solar heating is generally regarded as a clean and low-carbon heating method, while its high initial investment hinders its promotion in economically underdeveloped areas. With the implementation of the clean heating policy and the proposal of the carbon neutralization target, rural bulk coal heating in northern China is restricted. The Chinese government proposes to widely adopt solar heating to meet the heating demands of rural residents.