Targeted soluble epoxide hydrolase inhibits M1 macrophage polarization to improve cartilage injury in temporomandibular joint osteoarthritis.

Background: Macrophage immunomodulation has emerged as a novel intervention and therapeutic strategy for temporomandibular joint osteoarthritis (TMJOA), potentially serving as a key approach for reducing synovial inflammation and promoting cartilage repair. The soluble epoxide hydrolase inhibitor (sEHi), TPPU, has shown potential therapeutic effects against inflammatory diseases and osteogenesis by elevating endogenous Epoxyeicosatrienoic acids (EETs). However, it remains largely unknown whether TPPU can reduce inflammation and cartilage degradation in the TMJOA.

Spatiotemporal patterns and propagation of meteorological and hydrological drought in a humid basin of Southeast China.

Droughts have caused great damage due to climate change and intensified human activities. Quantifying the propagation law of meteorological drought (MD) to hydrological drought (HD) is essential for drought warning, defense and adaption. In this study, based on the standardized precipitation evapotranspiration index (SPEI) and standardized runoff index (SRI), we analyzed the spatiotemporal characteristics and propagation patterns of MD and HD 1975-2020 in Minjiang River Basin (MRB) located in Southeast China.

Melatonin as an Alleviator in Decabromodiphenyl Ether-Induced Aberrant Hippocampal Neurogenesis and Synaptogenesis: The Role of Wnt7a.

Developmental exposure to polybrominated diphenyl ethers (PBDEs), which are commonly used as flame retardants, results in irreversible cognitive impairments. Postnatal hippocampal neurogenesis, which occurs in the subgranular zone (SGZ) of the dentate gyrus, is critical for neuronal circuits and plasticity. Wnt7a-Frizzled5 (FZD5) is essential for both neurogenesis and synapse formation; moreover, Wnt signaling participates in PBDE neurotoxicity and also contributes to the neuroprotective effects of melatonin.

SH3-containing guanine nucleotide exchange factor (SGEF) ameliorates pressure overload induced cardiac hypertrophy via enhancing EGFR-NRF2 mediated ferroptosis inhibition.

SH3-containing guanine nucleotide exchange factor (SGEF) is reportedly associated with tumorigenesis. However, the role of SGEF in cardiovascular diseases such as cardiac hypertrophy has not been elucidated. Here, we used a pressure overload-induced cardiac hypertrophy mouse model to explore the role and underlying mechanism of SGEF in pathological cardiac hypertrophy.

Identification of oligonucleotide drug impurities using heart-cutting two-dimensional liquid chromatography-tandem mass spectrometry (2D-LC-MS/MS).

Background: Oligonucleotide-based drugs have gained significant attention as a promising class of therapeutics due to their precise ability to regulate gene expression. However, the presence of impurities in these drugs can compromise their safety, efficacy, and stability. Therefore, the identification and detailed analysis of these impurities are crucial.

Physical Aspects, Phytochemical Profiles, and Nutritional Properties of Lemon () Slices Under Different Drying Technologies.

Dried lemon slices (LSs) have become increasingly popular as a healthful beverage when infused in hot water. This study examined the effects of freeze drying (FD), hot air drying (HAD), heat pump drying (HPD), and far-infrared drying (FID) on the quality of dried LSs and their brewed beverages. The results show that FD-LSs and their corresponding beverages have the most appealing appearance and maximum levels of ascorbic acid (2.

Self-splicing RNA circularization facilitated by intact group I and II introns.

Circular RNA (circRNA) has gained significant attention in RNA therapeutics due to its enhanced stability and protein-coding potential. In this study, we present two in vitro RNA circularization techniques, namely Permuted Intron-Exon through Trans-splicing (PIET) and Complete self-splicing Intron for RNA Circularization (CIRC). PIET leverages the second step of group I intron splicing, offering an alternative circularization strategy.

Prognosis of Pediatric Dilated Cardiomyopathy: Nomogram and Risk Score Models for Predicting Death/Heart Transplantation.

This study aimed to develop a predictive model to assess risk factors and prognoses in pediatric patients with dilated cardiomyopathy (DCM). A total of 233 pediatric patients with DCM who were hospitalized between January 2019 and June 2024 were enrolled. The children were followed up and categorized into two groups: the death/heart transplantation (D/HT) group and the non-D/HT group.

Dual-mode sensing of Staphylococcus aureus in food via fluorescent Apt@Fe/Zr MOF with peroxidase-like activity.

Staphylococcus aureus, a prevalent foodborne pathogenic bacterium, poses a significant threat to human health. Herein, a dual-mode sensing method is proposed for detecting S. aureus in food using aptamer-modified Fe/Zr metal organic framework (Apt@Fe/Zr MOF).

Periodontitis-induced neuroinflammation triggers IFITM3-Aβ axis to cause alzheimer's disease-like pathology and cognitive decline.

Background: Periodontitis is a risk factor linked to Alzheimer's disease (AD), and characterized by amyloid-beta (Aβ) pathology. Mounting evidence suggests a contributory role of periodontitis in the onset and progression of AD. Type I interferons are upregulated in Porphyromonas gingivalis (Pg)-induced periodontitis in murine models.

Occludin Acts as a Dynein Adaptor Regulating Permeability and Collateral Angiogenesis.

Previous studies of the tight junction protein occludin (OCLN) suggest that multiple phosphorylation sites on the carboxy-terminal domain contribute a regulatory role in vascular barrier properties. However, gene deletion studies failed to identify a clear functional role for OCLN, despite multiple phenotypic alterations. Importantly, previous studies targeting exon 3 allowed expression of a splice variant starting at exon 4, (isoform 4) that expresses the full carboxy-terminal tail.

Cell-free miRNA-150-5p serves as a biomarker and regulator of PROM-related preterm labor by targeting chorionic ADAM19.

The prediction and treatment of spontaneous preterm labor (sPTL) are critical challenges due to obscure etiology and lack of highly specific and sensitive testing methods. Here, we extended the application of noninvasive prenatal testing into the field of sPTL prediction using high-throughput small RNA sequencing to screen the potential biomarkers for sPTL in maternal peripheral blood. We found that hsa-miR-150-5p and hsa-miR-512-3p decreased in the patients affected by sPTL, compared with either term labor or term not labor patients.

Regulation of partial endothelial-to-mesenchymal transition by circATXN1 in ischemic diseases.

Ischemic injury induces a partial mesenchymal shift in endothelial cells (ECs), contributing to impaired vascular regeneration. However, the molecular regulators of this transitional state remain poorly defined. To address this, we performed circular RNA profiling of endothelial cells under ischemic-like conditions and identified a marked upregulation of a circular RNA, named circATXN1.

Prediction of the 70-gene signature (MammaPrint) high versus low risk by nomograms among axillary lymph node positive (LN+) and negative (LN-) Chinese breast cancer patients, a retrospective study.

Background: Luminal-type breast cancer (BC) was characterized as hormonal receptor positive human epidermal receptor 2 negative (HR+/HER2-). The 70-gene signature (70-GS, MammaPrint) test is recommended for assessing recurrence risk and guiding adjuvant chemotherapy decisions for Luminal-type BC. This study updates previously established nomogram models to predict binary 70-GS risk for lymph node positive (LN+) and lymph node negative (LN-) luminal-type BC patients.

One-year longitudinal association between changes in aortic regional morphology and muscle mass in cancer.

Cancer confers the risk for cardiovascular diseases. This longitudinal study aimed to explore the relationship between changes in aortic morphology and muscle mass in cancer. One hundred patients with cancer who underwent thoracoabdominal enhanced computed tomography (CT) at baseline and 1-year follow-up were retrospectively included.

Molecular Mechanisms of var. Against Gastric Cancer: Metabolite Analysis, Signaling Pathways, and Protein Targets.

Ginseng ( var. ) is a traditional medicinal plant with anticancer properties. We aimed to assess the biological activity, potential targets, and molecular mechanisms of var.

[Genetic analysis of two fetuses with Mosaic variegated aneuploidy syndrome caused by compound heterozygous variants in BUB1B and its upstream regulatory elements and a literature Review].

Objective: To explore the genetic etiology of two fetuses with Mosaic variegated aneuploidy syndrome (MVA) in a pedigree.

Methods: A 30-year-old pregnant woman, who presented at the Center for Medical Genetics and Prenatal Diagnosis of Shandong Maternal and Child Health Care Hospital on November 16, 2023, was enrolled. Clinical data of the pedigree were collected, and peripheral blood samples from the parents and amniotic fluid samples from the two fetuses were obtained for genomic DNA extraction.

Measuring Managerial, Collegial, and Organizational Aspects Associated With Digital Health Competence in Healthcare Professionals: Validation of the Chinese Version of the DigiComInf Instrument.

Managerial, collegial, and organizational influences have been recognized as critical factors for developing digital health competence among healthcare professionals, but there is currently a lack of validated Chinese instruments to evaluate these factors. To culturally adapt and validate the Chinese version of the DigiComInf (aspects associated with digital health competence) instrument among Chinese healthcare professionals. The DigiComInf instrument was translated into Chinese following the established guidelines.

An Antagonism Between Ethylene Signaling and DNA Methylation Orchestrates the Progression of Leaf Senescence in Non-heading Chinese Cabbage.

Leaf senescence significantly impacts both the yield and quality of non-heading Chinese cabbage (NHCC). Despite its importance, the molecular mechanisms underlying the initiation and progression of leaf senescence in NHCC remain poorly understood. Here, transcriptome changes are first profiled, and found that ethylene (ET) and salicylic acid (SA) signaling pathways are activated during leaf senescence.

Validation of Chinese version of the familiar tools use test for assessing limb apraxia in stroke patients.

Objective: To validate the Familiar Tools Use Test (FTT) of the Diagnostic Instrument for Limb Apraxia-Short Version in Chinese stroke patients.

Methods: Participants were conveniently enrolled from a neurology ward in a tertiary hospital in Guangzhou, China, between April 2023 and September 2023. Internal consistency, test-retest reliability, inter-rater reliability, dimensionality, convergent validity, and divergent validity were examined.

Lewis Base-Catalyzed DYKAT of Racemic Chlorosilanes En Route to Si-Stereogenic Silazanes.

A dynamic kinetic asymmetric transformation (DYKAT) of racemic chlorosilanes into Si-stereogenic silazanes has been developed. The chiral isothiourea catalyst ()-benzotetramizole facilitates silylamination with sulfonamides, yielding ()-silazanes with ≥99:1 after one recrystallization. The approach is distinct from the conventional desymmetrization strategy using dihydrosilanes, expanding the synthetic toolbox for Si-stereogenic silazanes.

Indolepropionic acid modulates the immune response in allergic rhinitis through the AKT/CEBPB/IL‑10 signaling pathway.

Allergic rhinitis (AR) is a common inflammatory disorder of the nasal mucosa, usually triggered by environmental allergens. Indolepropionic acid (IPA) can influence immune responses; however, the specific mechanisms underlying the effects of IPA on immune regulation in AR remain largely unexplored. In the present study, an experimental mouse model of AR was established by sensitizing and exposing the mice to allergens, followed by the administration of IPA via gavage.