[Study on the mechanism of electroacupuncture in improving olfactory function in rats with allergic rhinitis-induced olfactory dysfunction through inhibition of inflammatory response].

Objectives: To observe the effects of electroacupuncture (EA) on olfactory function, olfactory epithelial cell apoptosis, olfactory marker protein (OMP), cysteinyl aspartate-specific protease-3 (Caspase-3), and the expression of inflammatory factors in rats with allergic rhinitis (AR)-induced olfactory dysfunction, so as to explore the underlying mechanism by which EA improves olfactory function in AR-induced olfactory dysfunction.

Methods: SD rats were randomly divided into control, model and EA groups, with 8 rats in each group. AR-induced olfactory dysfunction rats model was established using ovalbumin sensitization.

Factors Influencing Adherence to Dietary Interventions Among Patients with Gestational Diabetes Mellitus in China: A Qualitative Study Based on the COM-B Model.

Objective: Gestational diabetes mellitus (GDM) is a frequent complication during pregnancy, which can cause many adverse effects on mother and baby. Although existing literatures have found that patients with gestational diabetes can benefit from adhering to dietary interventions, many pregnant women exhibit low dietary intervention compliance, leading to unsatisfactory blood glucose control. The qualitative research study aimed to identify the influencing factors of dietary intervention compliance among pregnant women with GDM using the capability, opportunity, motivation, and behavior (COM-B) model.

Nutritional management adherence via an ePRO platform in patients with cancer: a machine learning model study.

Background: Electronic patient-reported outcome (ePRO) systems have significant potential for providing individualized and continuous nutritional management for patients with cancer. However, adherence to ePRO-guided nutritional interventions varies significantly, and the key factors associated with adherence remain poorly understood. This study aimed to assess adherence to nutritional targets via ePRO platforms and identify predictors influencing adherence to optimize management strategies.

Combining promiscuous enzymes to construct a platform cell factory which yields flavonol 3-O-galactoside 6-O-acetates.

Promiscuous enzymes are distributed widely in nature. These promiscuous enzymes recognize multiple substrates and yield a variety of metabolites. Hence, the enzyme promiscuity is usually avoided to improve the specificity of metabolites.

Acupoint injection and electrical stimulation for postoperative nausea and vomiting: A randomized clinical trial.

Objective: To assess the efficacy and safety of dexamethasone injection into the Neiguan acupoint combined with transcutaneous electrical acupoint stimulation (TEAS) on postoperative nausea and vomiting (PONV) in patients undergoing gynaecological laparoscopic surgery.

Study Design: This trial included 160 patients aged between 18 and 65 years who underwent gynaecological laparoscopic surgery. Participants were assigned at random to one of four groups - the acupoint injection (AI) + TEAS group, AI group, TEAS group or control group - in a 1:1:1:1 ratio.

Biosynthesis of a -Acetylated Tricyclic Carbazole with Antithrombotic Activity.

Tricyclic carbazoles are significant pharmacophores. Herein, heterologous expression of the carbazole-3,4-quinone () biosynthetic pathway in the chassis host J1074 yielded a previously chemosynthesized orthoquinone carbazole () and three new -acetylated carbazoles (-). Their structures were established by a combination of HR-ESI-MS, NMR, and X-ray crystallographic analysis.

Anti-Inflammatory α-Pyrone Polyethers Generated by Interrupting the Enolized 1,3-Diketone Formation in the Polyketide Synthases of Calcium-Ionophore Ionomycin.

Carboxyl polyethers from have diverse bioactivities, being biosynthesized by type-I polyketide synthetases. Of them, ionomycin, the commercial calcium ionophore, bears a unique enolized 1,3-diketone moiety. Herein, mutagenetic analysis of ionomycin biosynthesis revealed a rare dehydrogenase gene J putatively involved in enol moiety formation.

[Acupoint injection inhibiting abnormal secretion of mucin and inflammatory reaction of nasal mucosa in rats with allergic rhinitis through the p38 mitogen-activated protein kinase pathway].

Objectives: To observe the effects of acupoint injection on the expression of p38 mitogen-activated protein kinase (MAPK), phosphorylated (p)-p38 MAPK, mucin 5 subtype AC (MUC5AC) in the nasal mucosa, and serum inflammatory factors of rats with allergic rhinitis, so as to explore the mechanism of acupoint injection in improving inflammatory reactions in the nasal mucosa of rats with allergic rhinitis.

Methods: SD rats were randomly divided into the normal group, model group, acupoint injection group, and non-acupoint group, with 7 rats in each group. The allergic rhinitis rat model was established using ovalbumin sensitization.

[Multidimensional supportive psychological intervention in clinical practice teaching of andrological nursing].

Objective: To examine the application of a novel pedagogical approach multidimensional supportive psychological intervention (MSPI) in the clinical practice teaching of andrological nursing care.

Methods: Using the Hamilton Depression Scale (HAMD), we assessed the psychology of 100 nursing interns about to enter clinical practice in the Department of Andrology from December 2021 to December 2022. We equally randomized the subjects into an experimental and a control group, the former receiving MSPI and the latter trained on the conventional teaching model without any psychological support intervention.

Marcytoglobosins A and B, Cytochalasans From a Marine Sponge Associated Chaetomium globosum 162105 Fungus.

Two new cytochalasans, marcytoglobosins A (1) and B (2) were isolated from the marine sponge associated fungus Chaetomium globosum 162105, along with six known compounds (3-8). The complete structures of two new compounds were determined based on 1D/2D NMR and HR-MS spectroscopic analyses coupled with ECD calculations. All eight isolates were evaluated for their antibacterial activity.

Meroterpenoids from Marine Sponge sp. and Their Anticancer Activity against Human Colorectal Cancer Cells.

Two new meroterpenoids, hyrtamide A () and hyrfarnediol A (), along with two known ones, 3-farnesyl-4-hydroxybenzoic acid methyl ester () and dictyoceratin C (), were isolated from a South China Sea sponge sp. Their structures were elucidated by NMR and MS data. Compounds - exhibited weak cytotoxicity against human colorectal cancer cells (HCT-116), showing IC values of 41.

Mutagenetic analysis of the biosynthetic pathway of tetramate bripiodionen bearing 3-(2H-pyran-2-ylidene)pyrrolidine-2,4-dione skeleton.

Background: Natural tetramates are a family of hybrid polyketides bearing tetramic acid (pyrrolidine-2,4-dione) moiety exhibiting a broad range of bioactivities. Biosynthesis of tetramates in microorganisms is normally directed by hybrid polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) machineries, which form the tetramic acid ring by recruiting trans- or cis-acting thioesterase-like Dieckmann cyclase in bacteria. There are a group of tetramates with unique skeleton of 3-(2H-pyran-2-ylidene)pyrrolidine-2,4-dione, which remain to be investigated for their biosynthetic logics.

Characteristics and literature review of ETV6::ABL1 fusion gene-positive acute myeloid leukemia.

Objective: To describe the features of ETV6::ABL1 AML as well as the clinical treatment and outcomes.

Methods: Clinical data were collected from three patients diagnosed with ETV6::ABL1 AML at Hebei Yanda Lu Daopei Hospital and Beijing Lu Daopei Hospital. Their clinical and laboratory features were analyzed, and the treatment process and outcomes were described.

The novel HLA-B*40:555 allele identified by Sanger dideoxy nucleotide sequencing in a Chinese individual.

HLA-B*40:555 differs from HLA-B*40:01:02:01 by one nucleotide in exon 3.

Inhibition of PPP1R15A alleviates osteoporosis via suppressing RANKL-induced osteoclastogenesis.

Osteoporosis results from overactivation of osteoclasts. There are currently few drug options for treatment of this disease. Since the successful development of allosteric inhibitors, phosphatases have become attractive therapeutic targets.

Exploring 3-O-glycosylations of 20(R)-dammarane ginsenosides and the catalytic mechanism underlying the stereoselectivity with the combined assistance of AlphaFold 2 and molecular docking.

Glycosylation at C3-OH is the favorable modification for pharmaceutical activities and diversity expansion of 20(R)-dammarane ginsenosides. The 3-O-glycosylation, exclusively occurring in 20(R)-PPD ginsenosides, has never been achieved in 20(R)-PPT ginsenosides. Herein, 3-O-glycosylation of 20(R)-PPT enabled by a glycosyltransferase (GT) OsSGT2 was achieved with the combined assistance of AlphaFold 2 and molecular docking.

Ovariectomy induces hyperalgesia accompanied by upregulated estrogen receptor α and protein kinase B in the rat spinal cord.

Hormone supplementation is one of the common therapies for menopause-related disorders. Among different tools, the ovariectomy (OVX) rodents are widely accepted as an appropriate menopausal pain model. Our previous study has showed that OVX produces a significant pain facilitation in both acute pain and tonic pain, however, the underlying mechanisms remain unclear.

[Effect of catgut implantation on macrophage CD68, tumor necrosis factor-α and interleukin-1β in "Zusanli" (ST 36) region of rats].

Objective: To observe the expression of local macrophages and related cytokines tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) after catgut implantation in "Zusanli"(ST 36) in rats, so as to explore its underlying mechanisms in inducing therapeutic effect.

Methods: A total of 110 male SD rats were randomly divided into blank control group (=10), catgut embedding (CE) group (=50), and sham CE group (=50). The CE and sham CE groups were randomly divided into 8 h, 3 d, 7 d, 14 d and 21 d subgroups after the intervention (=10 in each time point group).

LC-MS-Guided Isolation of Cyanogripeptides A-C, Cyclolipopeptides with β-Methyl-Leucine Residues, from an LHW52806.

Cyanogripeptides A-C (-), three new cyclolipopeptides with unusual β-methyl-leucine residues, were identified from an LHW52806 using an LC-MS-guided strategy. The structures of compounds - were elucidated by 1D/2D NMR, HR-MS/MS, and the advanced Marfey's method. The absolute configuration of the β-methyl-leucine residue was determined by a combination of stereoselective biosynthesis of (2,3)-β-methyl-leucine, racemization to its epimer (2,3)-β-methyl-leucine, and the advanced Marfey's method.

Comparative Characterization of the Ginsenosides from Six Herbal Extracts and Their Rat Gut Microbial Metabolites by Advanced Liquid Chromatography-Mass Spectrometry Approaches.

Ginseng extracts are extensively used as raw materials for food supplements and herbal medicines. This study aimed to characterize ginsenosides obtained from six plant extracts (, red ginseng, , , , and var. ) and compared them with their metabolic profiles mediated by rat intestinal microbiota.

[Acupoint injection ameliorates Th1/Th2 imbalance through Toll-like receptor 4/activator protein-1 signal pathway and improves inflammatory response in rats with allergic rhinitis].

Objective: To observe the effect of acupoint injection on serum T helper (Th)1/Th2 related cytokines, and the expression levels of Toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88) and activator protein-1 (AP-1) of nasal mucosa in allergic rhinitis (AR) rats, so as to explore its mechanism underlying improvement of AR.

Methods: Thirty-two SD rats were randomly divided into normal, model, non-acupoint injection and acupoint injection groups (=8 in each group). The AR model was established by ovalbumin sensitization.