Trojan Horse-Inspired Biomimetic Lipoprotein Nanocarrier for Noninvasive Anti-VEGF Therapy of Ocular Fundus Neovascularization.

Pathological neovascularization is a major cause of vision loss in various ischemic and inflammatory ocular fundus diseases, with current anti-VEGF therapies requiring invasive and frequent intravitreal injections. In this study, a Trojan horse-inspired biomimetic lipoprotein nanocarrier (A-PA-αCPP) is developed for noninvasive anti-VEGF therapy via topical administration. Utilizing phosphatidic acid (PA), specifically 1,2-dioleoyl-sn-glycero-3-phosphate (DOPA), as the sole phospholipid, the nanocarrier enables electrostatic tethering of aflibercept, with a cell-penetrating peptide (CPP)-functionalized apoA1 mimetic peptide (αCPP) incorporated through self-assembly.

Deep learning and pathomics analyses predict prognosis of high-grade gliomas.

Objective: Utilizing pathomics to analyze high-grade gliomas and provide prognostic insights.

Methods: Regions of Interest (ROIs) in tumor areas were identified in whole-slide images (WSI). Tumor patches underwent cropping, white space removal, and normalization.

Effect of cooking condiments on swelling and starch-gluten matrix structural/molecular changes of cooked noodles.

Swelling and texture deterioration of cooked noodles are significant challenges hindering the growth of the takeout noodle market. This study investigated the swelling behavior and structural/molecular changes in the starch-gluten matrix of cooked noodles upon common condiments addition. Oil treatment significantly increased swelling by 56.

PPP1R3G inhibition impairs OPCs differentiation and myelination in aged mice.

Age-related cognitive decline is closely linked to white matter integrity, yet the molecular regulators of myelination during aging remain poorly defined. Protein phosphatase 1 regulatory subunit 3G (PPP1R3G) is implicated in cellular metabolism, but its role in brain aging is unknown. We assessed PPP1R3G expression in the corpus callosum of aging mice (1-18 months) and stratified aged mice into High/Low-PPP1R3G groups.

Effects of Organic Acidic Products from Discharge-Induced Decomposition of the FRP Matrix on ECR Glass Fibers in Composite Insulators.

This study investigates the degradation mechanisms of fiber-reinforced polymer (FRP) matrices in composite insulators under partial discharge (PD) conditions. The degradation products may further cause deterioration of the electrical and chemical resistance (ECR) glass fibers. Using pyrolysis-gas chromatography-mass spectrometry (PY-GC-MS) and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS), the thermal degradation gas and liquid products of the degraded FRP matrix were analyzed, revealing the presence of organic acids.

First Report of Infection in Pods of Four-Seeded Vetch and Its Relationships with Plants.

species are of great value in ecological restoration, soil improvement, and the development of a forage resource. In 2024, a novel pod disease affecting four-seeded vetches () emerged in Rongchang District, China, leading to severe yield loss. After obtaining the main pathogenic strain, FVS1, through the tissue isolation method, which was verified according to Koch's postulates, and by combining morphological characteristics with multigene phylogenetic analysis, FVS1 was identified as .

A prognostic model for head and neck squamous cell carcinoma based on radiotherapy sensitivity insights from nasopharyngeal carcinoma.

Background: Radioresistance significantly impairs treatment efficacy and prognostic outcomes in head and neck squamous cell carcinoma (HNSCC). This study aimed to identify radiotherapy sensitivity-related genes and construct a prognostic model for HNSCC, incorporating insights from nasopharyngeal carcinoma (NPC) as a related subtype.

Methods: Differentially expressed genes (DEGs) associated with radiotherapy response were identified using the GSE48501 dataset, primarily derived from NPC.

Lipoprotein-Based Nanocatalyst Enables Targeted Treatment of APAP-Induced Liver Injury via Enhanced Macropinocytosis.

Drug-induced liver injury (DILI), predominantly caused by acetaminophen (APAP) overdose, is characterized by excessive reactive oxygen species (ROS) production and subsequent hepatocyte necrosis. Although N-acetylcysteine (NAC) remains the only approved treatment, its effectiveness is limited by a narrow therapeutic time window and reduced efficacy in advanced cases. To address these limitations, an innovative therapeutic approach is developed utilizing ceria's antioxidant properties.

Relationship between 21 Medications and Negative Emotions: A Mendelian Randomization Analysis in Asian Populations.

Objective: Negative emotional states, such as nervousness, anxiety, depression, and tension, exert profound detrimental effects on an individual's quality of life and overall health. Although certain widely prescribed medications have been observed to modulate these emotional states, the existing body of research in this domain remains insufficient. To address this gap, Mendelian randomization (MR) methodologies, leveraging large-scale datasets, were employed to investigate the causal relationships between 21 commonly utilized medications and the manifestation of negative emotions.

The Value of Quantitative Ultrasound Elastography in the Assessment of Non-Alcoholic Fatty Liver Disease in Children.

Objective: This preliminary investigation aimed to assess the value of two elastography techniques, sound touch elastography (STE) and sound touch quantification (STQ), in measuring liver stiffness in children with non-fatty versus fatty livers.

Methods: This study used a case-control design. The STE and STQ were used to measure and compare liver stiffness in 121 children with fatty livers and 251 children with non-fatty livers, respectively.

Impact of thyroglobulin changes on clinical outcomes of differentiated thyroid cancer with biochemical incomplete response.

Purpose: Partial patients with biochemical incomplete response (BIR) after initial therapy for differentiated thyroid cancer (DTC) may progress to structural recurrence during follow-up. For better-individualized care, this study analyzed predictors of structural recurrence in patients with BIR after initial radioactive iodine therapy (RAIT).

Methods: A retrospective study was conducted on 4,263 patients with DTC who underwent thyroidectomy and initial RAIT between June 2009 and August 2023.

Radiomic analysis based on machine learning of multi-sequences MR to assess early treatment response in locally advanced nasopharyngeal carcinoma.

ObjectiveThe prediction of early response in locally advanced nasopharyngeal carcinoma (LA-NPC) after concurrent chemoradiotherapy (CCRT) is important for determining the need for timely consolidation therapy. We developed a radiomic analysis of multi-sequences MR based on machine learning (ML) to assess early response in LA-NPC after CCRT.MethodsThis study retrospectively enrolled 104 LA-NPC patients, randomly divided into training (70%) and test (30%) cohorts.

Direct Transformation of Bio-Based Furfural into γ-Valerolactone over SOH-Functionalized P─O─Hf Hybrid Catalysts.

γ-Valerolactone (GVL) has great application potential in organic synthesis and biofuels, while its high-efficiency production from easily available bio-based feedstock is challenging. In this work, SOH-functionalized P-O-Hf hybrid catalysts GPCS-Hf(n:m) were facilely prepared by solvothermal treatment of glucose-containing organic precursors (GPC) and Hf in a mass ratio of n:m. The acid strength and Lewis/Brønsted acid sites of GPCS-Hf(n:m) could be modulated by tuning the GPC/Hf ratio.

Bidirectional Mendelian Randomization Analysis to Study the Relationship Between Human Skin Microbiota and Radiation-Induced Skin Toxicity.

Radiation-induced skin toxicity, resulting from ionizing or nonionizing radiation, is a common skin disorder. However, the underlying relationship between skin microbiota and radiation-induced skin toxicity remains largely unexplored. Herein, we uncover the microbiota-skin interaction based on a genome-wide association study (GWAS) featuring 150 skin microbiota and three types of skin microenvironment.

The role of mitophagy-related genes in prognosis and immunotherapy of cutaneous melanoma: a comprehensive analysis based on single-cell RNA sequencing and machine learning.

Mitophagy, the selective degradation of mitochondria by autophagy, plays a crucial role in cancer progression and therapy response. This study aims to elucidate the role of mitophagy-related genes (MRGs) in cutaneous melanoma (CM) through single-cell RNA sequencing (scRNA-seq) and machine learning approaches, ultimately developing a predictive model for patient prognosis. The scRNA-seq data, bulk transcriptomic data, and clinical data of CM were obtained from publicly available databases.

Customized design of host-independent T7 expression system (HITES) for a broad host range.

Efficient methods and universal DNA elements are eagerly required for the expression of proteins and the production of target chemicals in synthetic biology and metabolic engineering. This paper develops a customized-design approach by utilizing the host-independent T7 expression system (HITES), which facilitates the rational design and rapid construction of T7 expression systems. Firstly, the EL (Upper-limit value of initial enzyme activity) value is discovered to play a pivotal factor in the successful construction of the T7 expression system, different host strains exhibit varying EL values, and this study presents a method to measure the EL values.

A nomogram for individualized prediction for cervical lymph node metastasis of papillary thyroid carcinoma.

Background: Cervical lymph node metastasis in papillary thyroid carcinoma plays a crucial role in the development of surgical strategy for thyroid patients. The aim of this study was to determine the predictors of cervical lymph node metastasis based on ultrasound features of papillary thyroid carcinoma, and to develop and validate nomogram to help predict cervical lymph node metastasis.

Methods: Patients who underwent thyroid ultrasound examination in Department of Ultrasonography of The First Affiliated Hospital of Nanjing Medical University between January 1, 2021 and October 31, 2021 were selected.

Polyphenol-Based Self-Assembled Nanomedicine for a Three-Pronged Approach to Reversing Tumor Immunosuppression.

The challenges of multi-pathway immune resistance and systemic toxicity caused by the direct injection of immune checkpoint inhibitors are critical factors that compromise the effectiveness of clinical immune checkpoint blockade therapy. In this context, natural polyphenols have been employed as the primary component to construct a targeted and acid-responsive PD-L1 antibody (αPD-L1) delivery nanoplatform. This platform incorporates garcinol, an inhibitor of the Nuclear Factor Kappa-B (NF-κB) signaling pathway, to regulate pro-tumor immune escape cytokines and regulatory T cells.

Case report: Characterizing of free-floating pigmented vitreous cyst using swept-source optical coherence tomography.

Aim: A free-floating vitreous cyst is a rare eye disease. This study aimed to find diagnostic imaging methods and imaging features for vitreous cysts.

Methods: This article presents a case report along with a literature review of published cases of vitreous cysts.

Anemarrhena asphodeloides Bunge total saponins lower lipid via modulating MAOA activity to enhance defense mechanisms in mice and C. elegans.

Ethnopharmacological Relevance: Within Anemarrhena asphodeloides Bunge (AAB), the pivotal bioactive constituents are identified as Anemarrhena asphodeloides Bunge total saponins (ABS). In traditional pharmacology, ABS has exhibited notable anti-inflammatory, hypoglycemic, and cardioprotective properties. Despite these observed effects, the specific protective mechanisms of ABS against metabolic diseases and improving the endocrine system remain largely uncharted.

Tumor habitat-based MRI features assessing early response in locally advanced nasopharyngeal carcinoma.

Objective: The early response to concurrent chemoradiotherapy in patients with locally advanced nasopharyngeal carcinoma (LA-NPC) is closely correlated with prognosis. In this study, we aimed to predict early response using a combined model that combines sub-regional radiomics features from multi-sequence MRI with clinically relevant factors.

Methods: A total of 104 patients with LA-NPC were randomly divided into training and test cohorts at a ratio of 3:1.

The WUSCHEL-related homeobox transcription factor negatively regulates fruit spine morphogenesis in cucumber ( L.).

Cucumber ( L.) is a widely cultivated crop with rich germplasm resources, holding significant nutritional value. It also serves as an important model for studying epidermal cell fate and sex determination.

A Network Meta-Analysis of the Systemic Therapies in Unresectable Head and Neck Squamous Cell Carcinoma.

Unlabelled: The current standard treatment for locally advanced squamous cell carcinoma of the head and neck (LASCCHN) comprises concurrent radiotherapy (CRT) alongside platinum-based chemotherapy. However, innovative therapeutic alternatives are being evaluated in phase II/III randomized trials. This study employed a Bayesian network meta-analysis (NMA) using fixed effects to provide both direct and indirect comparisons of all existing treatment modalities for unresectable LASCCHN.