Ammonia-induced dispersion and proliferation of Actinobacillus pleuropneumoniae biofilms.

Biofilm is a common status of bacteria persistent inside the host. Its dispersion can cause reactivation of bacteria, leading to disease outbreak. This study investigated ammonia's effects on Actinobacillus pleuropneumoniae, an important porcine respiratory pathogen.

Outcome of adding percutaneous transhepatic cholangial drainage to DEB-TACE in patients with unresectable cholangiocarcinoma with obstructive jaundice: comparison with sole DEB-TACE.

Background: Intrahepatic cholangiocarcinoma (ICC) with obstructive jaundice presents a therapeutic challenge, as most patients are ineligible for surgery. While FOLFOX chemotherapy offers limited survival benefits, drug-eluting bead transarterial chemoembolization (DEB-TACE) combined with percutaneous transhepatic cholangial drainage (PTCD) may improve outcomes by addressing both tumor burden and biliary obstruction.

Objective: To evaluate the efficacy and safety of DEB-TACE + PTCD versus DEB-TACE alone in unresectable ICC patients with obstructive jaundice.

Mobile phone dependency and subclinical depressive-anxiety symptom co-occurrence in college students: a cross-lagged panel network analysis.

Background: With the widespread use of smartphones among college students, issues related to smartphone dependence have become increasingly prevalent. Existing research has shown that subclinical anxiety and depression often co-occur and are closely associated with smartphone dependence. This study employed a cross-lagged panel network approach to explore the dynamic interplay between smartphone dependence and the co-occurrence of subclinical anxiety and depressive symptoms among college students.

Characterization and formation process analysis of peach fruit woolliness under low-temperature storage.

Woolliness in peach fruit during low-temperature storage considerably reduces quality, primarily owing to the formation of pectin gel. This study systematically analysed texture, water distribution, cell wall microstructure, and pectin gel formation during peach storage. Peaches stored at 5 °C exhibited increased firmness, membrane permeability, and water binding capacity than those at 10 °C.

Establishment and Verification of Risk Prediction Model for Adverse Outcomes After Hip Arthroplasty in Elderly Patients.

Background: Adverse outcomes after hip arthroplasty in elderly patients are frequently observed; however, most existing studies concentrate on single complications. Comprehensive predictive models for a wider range of adverse outcomes remain insufficient. This study explores this issue and proposes new approaches for clinical practice.

Experience of older adults using smart devices and mHealth apps in nursing homes in Chinese megacities: A descriptive qualitative study.

Objective: This study aims to explore the experiences of older adults in nursing homes located in megacities of China regarding the use of digital health tools. By examining their perceptions, challenges, and benefits, the study seeks to provide insights into how digital health technologies can be effectively integrated into elderly care in urban settings, ultimately contributing to improved health outcomes and quality of life for this population.

Methods: This study followed the Consolidated Criteria for Reporting Qualitative Research guidelines.

Multitask Deep Learning for Automated Segmentation and Prognostic Stratification of Endometrial Cancer via Biparametric MRI.

Background: Endometrial cancer (EC) is a common gynecologic malignancy; accurate assessment of key prognostic factors is important for treatment planning.

Purpose: To develop a deep learning (DL) framework based on biparametric MRI for automated segmentation and multitask classification of EC key prognostic factors, including grade, stage, histological subtype, lymphovascular space invasion (LVSI), and deep myometrial invasion (DMI).

Study Type: Retrospective.

Resveratrol reduces the activation of NLRP3 inflammasomes in rheumatoid arthritis through SIRT1 and ITGB αβ, especially in patients with high expression of ACPA.

Background: Rheumatoid arthritis (RA) is a chronic autoimmune disease with synovitis as the main pathological change. Previous research has demonstrated that the NLRP3 inflammasome is hyperactivated in RA patients, with its potential mechanism possibly linked to anti-citrullinated protein antibodies (ACPA). However, there are no RA treatment drugs specifically targeting NLRP3 inflammasome in clinical practice currently.

Added value of 3D fast-field-echo (FRACTURE) sequences for cervical spondylosis diagnosis: a prospective multi-reader non-inferiority study.

Objectives: To assess the potential of fast field echo resembling a CT using restricted echo-spacing (FRACTURE) sequence to enhance conventional MRI in detecting bone abnormalities of cervical spondylosis.

Materials And Methods: 137 consecutive patients with cervical spondylosis who underwent clinically indicated paired CT and MRI within 2 weeks between January and June 2024. After routine MRI, the 3D-FRACTURE sequences were performed.

In-feed provision of binding proteins sustains piglet gut health and mitigates ETEC-induced post-weaning diarrhea.

Background: Post-weaning diarrhea (PWD) in piglets, often caused by F4 enterotoxigenic Escherichia coli (ETEC), poses significant challenges in pig production. Traditional solutions like antibiotics and zinc oxide face increasing restrictions due to growing concerns over antibiotic resistance and environmental sustainability. This study investigates the application of bivalent heavy chain variable domain (VH) constructs (BL1.

Integrated analysis of methylomic and transcriptomic profiles in fetal mouse hypothalamus in response to maternal gestational exposure to arsenic.

Background: Gestational exposure to arsenic poses significant risks to fetal development by crossing the placental barrier and disrupting key physiological systems. Although animal studies suggest arsenic impairs hypothalamic development and neuroendocrine function, the precise molecular mechanisms remain poorly understood.

Methods: Pregnant ICR mice were divided into control and arsenic-exposed groups, with the exposed group receiving 4 mg/L of As₂O₃ in deionized water during gestation.

Streptococcus suis AdcA interacts with factor H and inhibits C3b deposition on the bacteria to participate in complement evasion.

Streptococcus suis is an important zoonotic pathogen. It can escape from the host complement attack through various strategies. In this study, the possible complement C3b interacted proteins of S.

ADL and Cognitive Function in Chinese Elderly: Mediating Role of Social Participation and Moderating Role of Intergenerational Support.

Purpose: This study aimed to explore the relationship between activities of daily living (ADL) and cognitive function in Chinese elderly, and to analyze in depth the mediating and moderating roles of social participation and children's intergenerational support in this relationship, in order to provide a reference basis for delaying cognitive decline in the elderly.

Patients And Methods: Based on data from the 2020 wave of China Health and Retirement Longitudinal Survey (CHARLS 2020), this study included 5612 subjects aged 60 years and older, and extracted main variables such as demographic information, ADL, social participation, cognitive function, and children's intergenerational support. Descriptive statistics and Pearson's correlation analysis in SPSS 27.

Construction of disability risk prediction model for the elderly based on machine learning.

The study aimed to develop a predictive model using machine learning algorithms, providing healthcare professionals with a novel tool for assessing disability risk in older adults. Data from the 2018 and 2020 waves of the China Health and Retirement Longitudinal Study were utilized, including 3,172 participants aged 65 years and older with no baseline disability. In this study, five machine learning algorithms were employed to construct risk assessment and prediction models for disability in older adults.

Construction and Validation of a Risk Prediction Model for Acute Gastrointestinal Injury in Non-ICU Elderly Critically Ill Patients.

Background: Acute gastrointestinal injury (AGI) has a relatively high prevalence among elderly critically ill patients in non-intensive care units (non-ICUs), and significantly influences their clinical outcomes. Therefore, it is important to identify people at risk for AGI and take preventive measures as early as possible.

Objective: We aimed to construct and validate a risk prediction model for AGI in non-ICU elderly critically ill patients.

The experience and need of patients with cauda equina syndrome caused by lumbar disc herniation: a phenomenological qualitative study.

Objective: Cauda equina syndrome (CES) is an emergency disease that can lead to serious sequelae. The purpose of this study was to deeply understand the experience and need of patients with CES caused by lumbar disc herniation (LDH).

Methods: Sixteen patients diagnosed as CES caused by LDH were enrolled.

Microdroplet Cascade Catalysis for Highly Selective Production of Propylene Glycol under Ambient Conditions.

Conventional propylene glycol (PG) production relies on an energy-intensive two-step thermocatalytic process, contributing significantly to CO emissions. A sustainable alternative under ambient conditions remains elusive, hindered by challenges in selectivity and energy efficiency. Here, we present a cascade catalysis strategy for efficient and selective PG production within water microdroplets under ambient conditions.

Effect of moderate hyperbilirubinemia on an infant's brain: a quantitative susceptibility mapping and H-MRS study.

Objective: The effects of moderate neonatal hyperbilirubinemia (NHB) remain unknown. The aim of this work was to investigate whether moderate NHB has an impact on an infant's brain and explore the relationship between brain magnetic susceptibility, brain metabolites, and biochemical tests in moderate NHB using quantitative susceptibility mapping (QSM) and magnetic resonance spectroscopy (MRS).

Materials And Methods: In total, 28 term babies with moderate levels of blood bilirubin were enrolled in the NHB group, and 16 term infants were enrolled in the control group.

Quantification of the Proton Density Fat Fraction and Iron Content: A Comparative Study Between 3.0 T and 5.0 T MRI.

Background: Proton density fat fraction (PDFF) and R2* are noninvasive MRI biomarkers for quantifying fat and iron in abdominal organs. While 3.0 T MRI is widely used clinically, 5.

Nat10-mediated N4-acetylcytidine modification enhances Nfatc1 translation to exacerbate osteoclastogenesis in postmenopausal osteoporosis.

Increased differentiation or activity of osteoclasts is the key pathogenic factor of postmenopausal osteoporosis (PMOP). N4-acetylcytidine (ac4C) modification, catalyzed by Nat10, is a novel posttranscriptional mRNA modification related to many diseases. However, its impact on regulating osteoclast activation in PMOP remains uncertain.

Single-cell multi-omics analysis identifies SPP1 macrophages as key drivers of ferroptosis-mediated fibrosis in ligamentum flavum hypertrophy.

Background: Ligamentum flavum hypertrophy (LFH) is a primary contributor to lumbar spinal stenosis. However, a thorough understanding of the cellular and molecular mechanisms driving LFH fibrotic progression remains incomplete.

Methods: Single-cell RNA sequencing (scRNA-seq) was performed to construct the single-cell map of human ligamentum flavum (LF) samples.

The Role of mRNA Modifications in Bone Diseases.

As a type of epigenetic modifications, mRNA modifications regulate the metabolism of mRNAs, thereby influencing gene expression. Previous studies have indicated that dysregulation of mRNA modifications is closely associated with the occurrence and progression of bone diseases (BDs). In this study, we first introduced the dynamic regulatory processes of five major mRNA modifications and their effects on the nucleus export, stability, and translation of mRNAs.

Screening of Anti-Hair Loss Plant Raw Materials Based on Reverse Network Pharmacology and Experimental Validation.

Hair loss is one of the skin conditions that can affect people's mental health. Plant raw material extracts are of great interest due to their safety. In this study, we utilize reverse network pharmacology to screen for key targets of the Wnt/β-catenin signaling pathway and the TGFβ/BMP signaling pathway, as well as key differential lipids, for plant raw materials selection.

Comprehensive analysis of the pituitary tumor-transforming gene (PTTG) family in lung adenocarcinoma: diagnostic, prognostic, and therapeutic implications.

Objectives: Lung adenocarcinoma (LUAD) is a prevalent form of non-small cell lung cancer with high morbidity and mortality rates. Identifying molecular markers and therapeutic targets is crucial for improving LUAD diagnosis and treatment. Pituitary tumor-transforming gene (PTTG) family members, PTTG1, PTTG2, and PTTG3P, have been linked to several malignancies; however, it is unclear how these genes relate to LUAD.