Verteporfin Mitigates Isoproterenol-Induced Myocardial Hypertrophy by Attenuating IL-6/STAT3 in Cardiac Fibroblasts.

Yes-associated protein (YAP) is a major downstream nuclear coactivator of the Hippo pathway and is activated during myocardial hypertrophy. Verteporfin, a YAP inhibitor, may serve as a potential treatment for myocardial hypertrophy. This study was aimed at exploring the role and underlying mechanisms of verteporfin in isoproterenol (ISO)-induced myocardial hypertrophy both in vivo and in vitro.

Alteration in conformation and emulsibility of lactoferrin-low methoxyl pectin complexes induced by non-covalent interactions with rosmarinic acid.

Protein-polysaccharide-phenolic acids ternary complexes exhibit unique structural and functional properties, yet the impact of phenolic acids on protein-polysaccharide complexes remains underexplored. This study investigates the non-covalently regulatory effect of rosmarinic acid (RA) on lactoferrin (LF)-low methoxyl pectin (LMP) complexes under various pH conditions. The alterations in particle size and zeta potential are pH-dependent and multi-spectral analysis indicates that the addition of RA leads to a more ordered protein structure.

Enhanced thermal stability of lactoferrin through electrostatic complexation with low-methoxyl pectin.

Lactoferrin (LF) possesses diverse bioactivities, but its application in food systems is limited due to environmental sensitivity and heat-induced denaturation. To improve its thermal stability, electrostatic complexes of LF with low-methoxyl pectin (LMP) were prepared, and their heat resistance was systematically investigated. Based on titration curves, soluble LF-LMP complexes at various mass ratios of 6:1, 4:1, and 2:1 (pH 6 and 7) were created.

Thyroid dysfunction and pulmonary arterial hypertension: A bidirectional mendelian randomization study.

Objective: Thyroid dysfunction can cause several cardiovascular diseases. Studies have suggested a link between thyroid dysfunction and an increased risk of Pulmonary Arterial Hypertension (PAH). However, the causal association between this link is unknown.

The activation of catecholamine neurons in the rostral ventrolateral medulla drives ventricular remodeling after myocardial ischemia/reperfusion injury.

Catecholamine neurons in the rostral ventrolateral medulla (RVLM) have long been recognized as a crucial neuronal population involved in cardiovascular regulation. However, its function and related circuits in ventricular remodeling after myocardial ischemia/reperfusion injury (MIRI) remain unclear. In this study, we investigated the potential role of RVLM catecholaminergic neurons and the underlying mechanisms that drive MIRI and the development of post-MIRI ventricular remodeling.

Partially substituting whey protein by rice protein hydrolysate to fabricate emulsions: Physicochemical properties, stability and digestion behavior.

The partial substitution of whey protein with plant-derived proteins in the formulation of new products has garnered significant attention. This study investigated emulsions stabilized by varying blends of whey protein concentrate (WPC) and rice protein hydrolysate (RPH) in mass ratios ranging from 100:0-0:100, focusing on their physicochemical properties, stability, and digestive characteristics. RPH was obtained via trypsin hydrolysis, achieving a solubility of up to 92.

BAG2 releases SAMD4B upon sensing of arginine deficiency to promote tumor cell survival.

Cells possess numerous metabolite sensors that detect essential nutrients for growth, with many directly binding to metabolites and responding to their levels. Given the vital role of arginine in various physiological and pathological processes, we hypothesized that there may be undiscovered sensors that detect arginine deficiency. Through a series of unbiased screening strategies in human cancer cell line models, we identified Bcl2-associated athanogene (BAG) family molecular chaperone regulator 2 (BAG2) as an arginine sensor, which could directly bind to arginine at the glutamine residue 167 (Q167).

Fabrication, characterization, and application in 3D printing of high internal phase emulsion stabilized by alkaline thermal treated pea protein and pectin complex.

Alkaline thermally treated pea protein and pectin complex was utilized to create high internal phase emulsions (HIPE) for extrusion-based 3D printing. Pea protein heated at pH 10 and 100 °C was optimized and chosen based on criteria such as solubility, surface hydrophobicity, particle size, and surface charge. The most promising ink for 3D printing was selected from HIPE stabilized by complexes containing 2 % heated pea protein with varying pectin concentrations (0.

Fabrication and characterization of glucono-δ-lactone-induced cold-set emulsion gels stabilized by a whey and pea protein mixture at various mixing ratios for the delivery of curcumin.

In this study, glucono-δ-lactone-induced cold-set emulsion gels stabilized by whey protein (WPI) and pea protein (PPI) at various mass ratios were formulated, characterized, and applied. Compared with WPI-stabilized emulsion, zeta potential of emulsion decreased but droplet size, apparent viscosity, and modulus increased with increasing PPI. Differences in the properties of emulsions led to differences in the structure and properties of gels formed.

A Novel Homozygous Nonsense Mutation in TACSTD2 Gene Causes Gelatinous Drop-like Corneal Dystrophy in a Chinese Consanguineous Family: A Case Report and Literature Review.

Purpose: To report a case of gelatinous drop-like corneal dystrophy (GDLD) caused by a novel homozygous tumor-associated calcium signal transducer 2 ( TACSTD2 ) gene mutation and summarize the correlations of clinical phenotypes and genotypes in patients with GDLD.

Methods: We analyzed the clinical characteristics and treatment outcomes of a 42-year-old man with GDLD. The patient with GDLD received multiple keratoplasties, and histopathological staining of the corneal sections was performed.

Influence of maternal lipid levels on adverse pregnancy outcomes in women with gestational diabetes mellitus.

Objective: This study aimed to investigate the effect of mid-pregnancy lipid levels on adverse outcomes in women with gestational diabetes mellitus (GDM) under adequate glycemic control. Whether this effect is independent of factors such as blood glucose was also analyzed.

Methods: We retrospectively analyzed 1,001 women with normal glucose tolerance (NGT) and 1,078 women with GDM under adequate glycemic control from 2015 to 2024.

Circular RNA CDYL facilitates hepatocellular carcinoma stemness and PD-L1 exosomes-mediated immunotherapy resistance via stabilizing hornerin protein by blocking synoviolin 1-mediated ubiquitination.

Despite the revolutionary progress in cancer immunotherapy, only a minority of hepatocellular carcinoma (HCC) patients respond to immune checkpoint inhibitors (ICIs). In this study, we found that the oncogenic circular RNA Circ-CDYL in HCC influences the efficacy of immunotherapy and the stemness characteristics of HCC cells by interacting with the hornerin (HRNR) protein. The degraded anti-PD-L1 immunotherapy responses induced by Circ-CDYL and HRNR were confirmed by peripheral blood mononuclear cells (PBMCs) killing assays in HCC cells, patient-derived organoids, and humanized immune system mouse models.

Partially substituting cheese base with plant proteins to produce processed cheese: Textural, rheological, microstructural and sensory properties.

The increasing demand for plant-based dairy alternatives, driven by sustainability and health concerns, has driven the development of hybrid processed cheeses (PC) that incorporate plant proteins. This study investigates the effects of replacing 5% of the dairy component (Mozzarella cheese base) with soy protein isolate (SPI), pea protein isolate (PPI), or mung bean protein isolate (MBPI) on the textural, rheological, microstructural, sensory properties of hybrid PC. Textural analysis revealed that hybrid PC with SPI exhibited the highest firmness and chewiness, attributed to a dense and cohesive protein network.

Effect of moderate hyperbilirubinemia on an infant's brain: a quantitative susceptibility mapping and H-MRS study.

Objective: The effects of moderate neonatal hyperbilirubinemia (NHB) remain unknown. The aim of this work was to investigate whether moderate NHB has an impact on an infant's brain and explore the relationship between brain magnetic susceptibility, brain metabolites, and biochemical tests in moderate NHB using quantitative susceptibility mapping (QSM) and magnetic resonance spectroscopy (MRS).

Materials And Methods: In total, 28 term babies with moderate levels of blood bilirubin were enrolled in the NHB group, and 16 term infants were enrolled in the control group.

Genome-Wide Identification and Expression Analysis of CrRLK1-like Gene Family in Potatoes ( L.) and Its Role in PAMP-Triggered Immunity.

Background: The receptor-like kinase 1-like (CrRLK1L) subfamily, a specialized group within receptor-like kinases (RLKs), was initially identified in cell cultures. CrRLK1L plays an important role in the growth, development and stress response of plants. Although CrRLK1L genes have been characterized across multiple plant species, their biological and genetic functions in potatoes () remains poorly elucidated.

Yersiniabactin produced by promotes intestinal inflammation through lipid peroxidation and ferroptosis.

(), a major foodborne pathogen, poses significant risks to public health by causing gastrointestinal diseases. Among its virulence factors, Yersiniabactin (Ybt), a siderophore, plays a crucial role in iron acquisition and enhancing intestinal colonization. Despite previous studies highlighting -Ybt's involvement in inflammation, its exact mechanisms remain unclear.

Escherichia coli HPI-induced duodenitis through ubiquitin regulation of the TLR4/NF-κB pathway.

Background: The Highly Pathogenic Island (HPI) found in Yersinia pestis can be horizontally transferred to E. coli, enhancing its virulence and pathogenicity. Ubiquitin (Ub) acts as an activator of the NF-κB pathway and plays a critical role in the inflammatory response.

Formation of whey protein, pectin, and chlorogenic acid ternary complexes and their application in emulsions.

Physicochemical properties, stability, and digestive behavior of lycopene-loaded emulsions prepared by ternary complexes fabricated with different mixing sequences based on whey protein isolate (WPI), high methoxyl pectin (HMP), and chlorogenic acid (CA) were investigated. Spectroscopic and molecular docking analyses confirmed the non-covalent interactions among the compounds within the ternary complexes, as well as the conformational changes in the protein induced by the mixing sequence. The interfacial tension (6.

Effects of mixing ratio on physicochemical, structural properties and application in lycopene-loaded emulsions of blends of whey protein and pea protein.

Physicochemical, structural properties and application in lycopene-loaded emulsions of blends of whey protein isolate (WPI) and pea protein isolate (PPI) at varying mass ratios (100/0, 75/25, 50/50, 25/75, 0/100) were investigated. Data indicated that the mass ratios affected the physical, chemical and storage stability of the emulsion by influencing the particle size, zeta-potential, surface hydrophobicity, free sulfhydryl content, and secondary structure of the blends. Particularly, emulsion with a mixing ratio of 75/25 exhibited superior physical stability against salt concentrations (200 and 500 mM), better chemical stability against UV light and heat, and maintained stability over a 30-day storage period.

Development and Validation of a Prediction Model for Thyroid Dysfunction in Patients During Immunotherapy.

Objective: This study was designed to develop and validate a predictive model for assessing the risk of thyroid toxicity following treatment with immune checkpoint inhibitors.

Methods: A retrospective analysis was conducted on a cohort of 586 patients diagnosed with malignant tumors who received programmed cell death 1 (PD-1)/programmed death-ligand 1 (PD-L1) inhibitors. The patients were randomly divided into training and validation cohorts in a 7:3 ratio.

Physicochemical Properties, Stability, and Functionality of Non-Covalent Ternary Complexes Fabricated with Pea Protein, Hyaluronic Acid and Chlorogenic Acid.

The aim of this study was to prepare and characterize stable non-covalent ternary complexes based on pea protein (PP, 0.5%), hyaluronic acid (HA, 0.125%), and chlorogenic acid (CA, 0~0.

Effect of SCN1Aand SCN2A gene polymorphisms on the efficacy of valproic acid treatment in Chinese children with epilepsy.

Objective: Epilepsy patients exhibit considerable differences in their response to sodium valproate (VPA) therapy, a phenomenon that might be attributed to individual genetic variances. The role of genetic variations, specifically in sodium channels encoded by SCN1A and SCN2A genes, in influencing the effectiveness of VPA in treating epilepsy is still debated. This research focuses on examining the impact of these genetic polymorphisms on the efficacy of VPA therapy among pediatric epilepsy patients in China.