Establishment and Verification of Risk Prediction Model for Adverse Outcomes After Hip Arthroplasty in Elderly Patients.

Background: Adverse outcomes after hip arthroplasty in elderly patients are frequently observed; however, most existing studies concentrate on single complications. Comprehensive predictive models for a wider range of adverse outcomes remain insufficient. This study explores this issue and proposes new approaches for clinical practice.

Experience of older adults using smart devices and mHealth apps in nursing homes in Chinese megacities: A descriptive qualitative study.

Objective: This study aims to explore the experiences of older adults in nursing homes located in megacities of China regarding the use of digital health tools. By examining their perceptions, challenges, and benefits, the study seeks to provide insights into how digital health technologies can be effectively integrated into elderly care in urban settings, ultimately contributing to improved health outcomes and quality of life for this population.

Methods: This study followed the Consolidated Criteria for Reporting Qualitative Research guidelines.

Nitrosarchaeum haohaiensis sp. Nov. CL1: Isolation and Characterisation of a Novel Ammonia-Oxidising Archaeon From Aquatic Environments.

Following a 3.5-year enrichment cultivation period, a novel ammonia-oxidising archaeon (AOA), designated strain CL1, was isolated from Yangshan Harbour (East China Sea). Strain CL1 demonstrates a maximum ammonia tolerance of up to 10 mM.

Live birth rates in IVF patients with and without polycystic ovary syndrome according to serum anti-Müllerian hormone concentrations.

Research Question: Is there a relationship between serum anti-Müllerian hormone (AMH) concentration and live birth rate (LBR) in patients with and without polycystic ovary syndrome (PCOS)?

Design: This retrospective study included 4631 patients aged 20-35 years who underwent their first frozen embryo transfer cycle at the First Affiliated Hospital of Zhengzhou University from June 2016 to June 2021. Participants were categorized as having or not having PCOS (PCOS group, n = 1428, and NPCOS group, n = 3203, respectively). In the two groups, AMH was categorized into subgroups 1, 2 and 3 using the 25th and 75th percentiles as cut-off lines.

Construction of disability risk prediction model for the elderly based on machine learning.

The study aimed to develop a predictive model using machine learning algorithms, providing healthcare professionals with a novel tool for assessing disability risk in older adults. Data from the 2018 and 2020 waves of the China Health and Retirement Longitudinal Study were utilized, including 3,172 participants aged 65 years and older with no baseline disability. In this study, five machine learning algorithms were employed to construct risk assessment and prediction models for disability in older adults.

A novel technique with cool-tip radiofrequency ablation for selective fetal reduction in complicated monochorionic twin.

Objective: To invent a novel method for selective fetal reduction in monochorionic (MC) twin using cool-tip radiofrequency ablation (RFA) and analysis the perinatal outcome.

Material And Methods: Complicated MC twins including twin-to twin transfusion syndrome (TTTS), selective fetal growth restriction (sFGR) and twin reverse arterial perfusion sequence (TRAP) were enrolled from 2020 to 2024. All cases were indicated for selective fetal reduction due to expected poor outcome.

Intrinsic Capacity and Instrumental Activity of Daily Living Among Community Dwelling Elderly in China: The Mediating Effect of Two-Way Social Support and Sense of Coherence.

Objectives: To explore the influence of intrinsic capacity (IC) on instrumental activities of daily living (IADL) among community-dwelling elderly and to analyze the chain-mediated effects of two-way social support, and sense of coherence (SOC).

Design: This study was a cross-sectional research design. Participants were recruited from September 2023 to December 2023 from Hangzhou City, Zhejiang Province, China, using a multistage sampling method.

Hernandezine acts as a CDK4 suppressor inhibiting tumor growth by the CDK4/PKM2/NRF2 axis in colon cancer.

Background: The cyclin-dependent kinase 4 (CDK4) interacts with its canonical and non-canonical substrates modulating the cell cycle in tumor cells. However, the potential substrates and the beyond-cell-cycle-regulated functions of CDK4 in colon cancer (CC) are still unknown. Hernandezine (HER) is previously verified to induce G0/G1 phase arrest and autophagic cell death in human cancer cells, which implies that HER might target G0/G1 phase-related proteins, including CDK4.

Construction of an Exercise Intervention Program for Patients with Sarcopenic Obesity: A Delphi Method Study.

Purpose: Construct an exercise intervention program for patients with sarcopenic obesity.

Material And Methods: Based on the COM-B theoretical model and evidence-based principles, the program was constructed using qualitative methods of literature analysis and Delphi method. The Delphi panel consisted of 15 experts from the fields of clinical medicine, rehabilitation medicine, medical technology, and nursing.

Sample multiplexing for retinal single-cell RNA-sequencing.

Rare cell populations can be challenging to characterize using microfluidic single-cell RNA sequencing (scRNA-seq) platforms. Typically, the population of interest must be enriched and pooled from multiple biological specimens for efficient collection. However, these practices preclude the resolution of sample origin together with phenotypic data and are problematic in experiments in which biological or technical variation is expected to be high (e.

Pregnancy outcomes in infertile patients with endometrial hyperplasia with or without atypia undergoing fertilization: the early-follicular long protocol is superior to midluteal long protocol.

Background: Although fertilization (IVF) in infertile patients with endometrial hyperplasia is common after drug treatment, the pregnancy outcomes are often unsatisfactory. Till date, no studies have reported the outcome of patients with endometrial hyperplasia treated using early-follicular long (EL) protocol and midluteal long (ML) protocol.

Objective: To evaluate the pregnancy outcomes and disease prognosis of patients with endometrial hyperplasia with or without atypia undergoing IVF treatment with EL protocol or ML protocol.

Forty-nine metagenomic-assembled genomes from an aquatic virome expand by 45 potential new families and the newly uncovered Gossevirus of .

Twenty complete genomes (29-63 kb) and 29 genomes with an estimated completeness of over 90 % (30-90 kb) were identified for novel dsDNA viruses in the Yangshan Harbor metavirome. These newly discovered viruses contribute to the expansion of viral taxonomy by introducing 46 potential new families. Except for one virus, all others belong to the class .

Extraction-free, immuno-RPA-CRISPR/Cas13a-based one-pot detection of glypican-3 directly from extracellular vesicles.

Background: Glypican-3 (GPC3) is a heparan sulfate proteoglycan (HSPG) that binds to the cell membrane via glycosylphosphatidylinositol (GPI). It is not found in healthy adult liver but is overexpressed in human hepatocellular carcinoma (HCC). The protein marker GPC3 on extracellular vesicles (GPC3 EVs) is also useful for HCC detection.

Relationship of environmental exposure temperature and temperature extremes on sperm DNA fragmentation index in men with different BMI values and the indirect effect of DNA fragmentation index on semen parameters.

Prior studies have established a significant correlation between the DNA fragmentation index (DFI) and infertility. Additionally, certain investigations suggest that environmental exposure may serve as an etiological factor impacting semen quality. This study aimed to explore the impact of season, ambient temperature, and weather extremes on the DFI of sperm, along with other relevant parameters.

S-1 and 5-Fluorouracil-related adverse events in patients with advanced gastric cancer: A meta-analysis.

Objective: To assess S-1 and 5-fluorouracil (5-FU)-related adverse events in patients with advanced gastric cancer and provide focused health care approaches to improve patient compliance and quality of survival.

Methods: The PubMed, Web of Science, Medline, Cochrane Library, EMbase, SinoMed, Wan Fang Data, CNKI, and VIP databases were searched, and relevant literature was screened from the database construction date through March 31, 2023. Review Manager 5.

Effects of non-pharmacological interventions on patients with sarcopenic obesity: A meta-analysis.

Objective: Systematic evaluation of the improvement effect of non-pharmacological intervention on patients with sarcopenic obesity.

Methods: Wanfang, VIP, China National Knowledge Infrastructure (CNKI), SinoMed, Web of Science, Cochrane Library, PubMed, and Embase databases were searched systematically for randomized controlled trials (RCTS) and experimental studies of non-pharmacological interventions on patients with sarcopenic obesity. The retrieval period was from the establishment of the databases to October 31, 2022.

Diversity, Relationship, and Distribution of Virophages and Large Algal Viruses in Global Ocean Viromes.

Virophages are a group of small double-stranded DNA viruses that replicate and proliferate with the help of the viral factory of large host viruses. They are widely distributed in aquatic environments but are more abundant in freshwater ecosystems. Here, we mined the Global Ocean Viromes 2.

Potential Role of Lysine Acetylation and Autophagy in Brown Film Formation and Postripening of Lentinula edodes Mycelium.

Lentinula edodes is one of the most widely cultivated edible mushrooms in the world. When cultivated in sawdust, the surface mycelium of needs a long postripening stage wherein it forms a brown film (BF) by secreting and accumulating pigments. BF formation is critical for the high quality and yield of fruiting bodies.

Shen-Qi-Jiang-Tang granule ameliorates diabetic nephropathy via modulating tumor necrosis factor signaling pathway.

Ethnopharmacological Relevance: Shen-Qi-Jiang-Tang granule (SQJTG), a classic traditional Chinese medicine (TCM) prescription, has been widely used in clinical for diabetes, especially type Ⅱ diabetes. Previous anti-diabetic studies stumbled across that SQJTG has a potential kidney protective effect on diabetic nephropathy (DN). However, the protective mechanism of SQJTG on DN still needs to be explored.

The circHMGCS1-miR-205-5p-ErBB3 axis mediated the Sanggenon C-induced anti-proliferation effects on human prostate cancer.

Prostate cancer (PCa) is associated with a high incidence and metastasis rate globally, resulting in an unsatisfactory prognosis and a huge economic burden due to the current deficient of therapeutic strategies. As the most abundant component of Cortex Mori, Sanggenon C (SC) is well known to possess bioactivities in tumors, but its mechanism is poorly understood. Consequently, we attempted to investigate whether SC could modulate circular RNA(s) levels and hence anti-PCa development.

Gut microbiota dysbiosis as an inflammaging condition that regulates obesity-related retinopathy and nephropathy.

Diabetes-specific microvascular disease is a leading cause of blindness, renal failure and nerve damage. Epidemiological data demonstrated that the high morbidity of T2DM occurs as a result of obesity and gradually develops into serious complications. To date, the mechanisms that underlie this observation are still ill-defined.

EDTA Combined with C-Pilot Files and Microultrasound for Root Canal Calcification: Dredging Effect and Safety Analysis.

Objective: This paper discussed the dredging effect and safety of ethylenediaminetetraacetic acid (EDTA) combined with C-pilot files and microultrasound (mUS) on root canal calcification.

Methods: From October 2021 to January 2022, 132 patients with calcified root canals treated in our hospital were selected as the research subjects. Among them, 64 cases who received EDTA combined with C-pilot Files and mUS plus ultrasonic instruments to dredge calcified root canals were regarded as the research group (RG), and another 68 cases given ultrasonic instruments plus C-pilot files were regarded as the control group (CG).

Is early-follicular long-acting GnRH agonist protocol an alternative for patients with polycystic ovary syndrome undergoing in vitro fertilization?

Background: We aimed to compare the clinical and perinatal outcomes of patients with polycystic ovary syndrome (PCOS) undergoing in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) treatment with either an early-follicular long-acting gonadotropin-releasing hormone agonist (GnRH-a) long protocol (EFLL) or a midluteal short-acting GnRH-a long protocol (MLSL).

Methods: This single-center, retrospective study, included patients with PCOS who underwent IVF/ICSI from January 2013 to June 2019 at the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. Patients underwent either MLSL (1179 cycles) or EFLL (2390 cycles).