Asymmetric Coordination Strategy of Cu Single-Atom Catalyst for Robust all-pH Oxygen Reduction Reaction.

Although Cu-N-C single-atom catalysts (SACs) is proven to be a potential substitute for oxygen reduction reaction (ORR), the rigid coordination structure of Cu-N active sites hampers mass transfer and electron transport during the ORR process, limiting their catalytic activity. In this study, an asymmetric coordination strategy (Cu-N-C/Cl) is implemented by doping chlorine, which subtly modulates the electronic structure of the Cu-N coordination environment in two dimensions. The Cu-N-C/Cl electrocatalysts with the optimized electronic structure exhibit outstanding ORR activity across all pH ranges with the half-wave potentials of 0.

Microdroplet-Engineered Skeletal Muscle Organoids from Primary Tissue Recapitulate Parental Physiology with High Reproducibility.

Achieving high maturity and functionality in in vitro skeletal muscle models is essential for advancing our understanding of muscle biology, disease mechanisms, and drug discovery. However, current models struggle to fully recapitulate key features such as sarcomere structure, muscle fiber composition, and contractile function while also ensuring consistency and rapid production. Adult stem cells residing in muscle tissue are known for their powerful regenerative potential, yet tissue-derived skeletal muscle organoids have not been established.

Altering the Symmetry of Fe-N-C by Axial Cl-Mediation for High-Performance Zinc-Air Batteries.

Fe-N-C catalyst is acknowledged as a promising alternative for the state-of-the-art Pt/C in oxygen reduction reaction (ORR) toward cutting-edge electrochemical energy conversion/storage applications. Herein, a "Cl-mediation" strategy is proposed on Fe-N-C for modulating the catalyst's electronic structure toward achieving remarkable ORR activity. By coordinating axial Cl atoms to iron phthalocyanine (FePc) molecules on carbon nanotubes (CNTs) matrix, a Cl-modulated Fe-N-C (FePc-Cl-CNTs) catalyst is synthesized.

Icariin inhibits hyperglycemia-induced cell death in penile cavernous tissue and improves erectile function in type 1 diabetic rats.

Background: Hyperglycemia can cause endothelial cell (EC) and smooth muscle cell (SMC) death in the penile cavernous tissue of rats and lead to erectile dysfunction (ED).

Objectives: To investigate the proportions of apoptotic, pyroptotic, and ferroptotic cells among ECs and SMCs in the penile cavernous tissue of type 1 diabetic (T1DM) rats and the mechanism by which icariin (ICA) improves the erectile function of T1DM rats.

Methods: A total of 24 9-week-old Sprague-Dawley (SD) rats were randomly divided into 4 groups ( = 6): control group, control + ICA group, diabetic mellitus (DM) group, and DM + ICA group.

Co-Motif-Engineered RuO Nanosheets for Robust and Efficient Acidic Oxygen Evolution.

The development of efficient and reliable acidic oxygen evolution reaction (OER) electrocatalysts represents a crucial step in the process of water electrolysis. RuO, a benchmark OER catalyst, suffers from limited large-scale applicability due to its tendency toward the less stable lattice oxygen mechanism (LOM). This work reports the synthesis of Co-doped RuO nanosheets with a unique porous morphology composed of interconnected grains via a facile molten salt method.

Bridging the organoid translational gap: integrating standardization and micropatterning for drug screening in clinical and pharmaceutical medicine.

Synthetic organ models such as organoids and organ-on-a-chip have been receiving recognition from administrative agencies. Despite the proven success of organoids in predicting drug efficacy on laboratory scales, their translational advances have not fully satisfied the expectations for both clinical implementation and commercial applications. The transition from laboratory settings to clinical applications continues to encounter challenges.

Identification of Nicotinic Acetylcholine Receptor for N-Acetylcysteine to Rescue Nicotine-induced Injury Using Beating Cilia in Primary Tissue Derived Airway Organoids.

Smoking is one of the major contributors to airway injuries. N-acetylcysteine (NAC) has been proposed as a treatment or preventive measure for such injuries. However, the exact nature of the smoking-induced injury and the protective mechanism of NAC are not yet fully understood.

Chronotoxici-Plate Containing Droplet-Engineered Rhythmic Liver Organoids for Drug Toxicity Evaluation.

The circadian clock coordinates the daily rhythmicity of biological processes, and its dysregulation is associated with various human diseases. Despite the direct targeting of rhythmic genes by many prevalent and World Health Organization (WHO) essential drugs, traditional approaches can't satisfy the need of explore multi-timepoint drug administration strategies across a wide range of drugs. Here, droplet-engineered primary liver organoids (DPLOs) are generated with rhythmic characteristics in 4 days, and developed Chronotoxici-plate as an in vitro high-throughput automated rhythmic tool for chronotherapy assessment within 7 days.

Circadian modulation of glucose utilization via CRY1-mediated repression of Pdk1 expression.

Life adapts to daily environmental changes through circadian rhythms, exhibiting spontaneous oscillations of biological processes. These daily functional oscillations must match the metabolic requirements responding to the time of the day. We focus on the molecular mechanism of how the circadian clock regulates glucose, the primary resource for energy production and other biosynthetic pathways.

Synthetic Biohybrids of Red Blood Cells and Cascaded-Enzymes@ Metal-Organic Frameworks for Hyperuricemia Treatment.

Hyperuricemia, caused by an imbalance between the rates of production and excretion of uric acid (UA), may greatly increase the mortality rates in patients with cardiovascular and cerebrovascular diseases. Herein, for fast-acting and long-lasting hyperuricemia treatment, armored red blood cell (RBC) biohybrids, integrated RBCs with proximal, cascaded-enzymes of urate oxidase (UOX) and catalase (CAT) encapsulated within ZIF-8 framework-based nanoparticles, have been fabricated based on a super-assembly approach. Each component is crucial for hyperuricemia treatment: 1) RBCs significantly increase the circulation time of nanoparticles; 2) ZIF-8 nanoparticles-based superstructure greatly enhances RBCs resistance against external stressors while preserving native RBC properties (such as oxygen carrying capability); 3) the ZIF-8 scaffold protects the encapsulated enzymes from enzymatic degradation; 4) no physical barrier exists for urate diffusion, and thus allow fast degradation of UA in blood and neutralizes the toxic by-product H O .

Surfactant-Mediated Crystalline Structure Evolution Enabling the Ultrafast Green Synthesis of Bismuth-MOF in Aqueous Condition.

Green synthesis of stable metal-organic frameworks (MOFs) with permanent and highly ordered porosity at room temperature without needing toxic and harmful solvents and long-term high-temperature reactions is crucial for sustainable production. Herein, a rapid and environmentally friendly synthesis strategy is reported to synthesize the complex topological bismuth-based-MOFs (Bi-MOFs), [Bi(CHO)(HO)] (denoted CAU-17), in water under ambient conditions by surfactant-mediated sonochemical approach, which could also be applicable to other MOFs. This strategy explores using cetyltrimethylammonium bromide (CTAB) amphiphilic molecules as structure-inducing agents to control the removal of non-coordinated water (dehydration) and enhance the degree of deprotonation of the ligands, thereby regulating the coordination and crystallization in aqueous solutions.

Versatile Multi-Wavelength Light-Responsive Metal-Organic Frameworks Micromotor through Porphyrin Metalation for Water Sterilization.

Traditional metal-organic frameworks (MOFs) based micro/nanomotors (MOFtors) can achieve three-dimensional (3D) motion mainly depending on noble metal (e.g., Pt), toxic fuels (e.

Microfluidic droplet encapsulation-guided organoid growth promotes parental tumor phenotype recapitulation.

Patient-derived organoids are gaining incremental popularity in both basic sciences and translational applications toward precision medicine and revolutionized drug discovery. However, for tumor organoids, challenges remain in low rates of organoid growth and tumor cell purity, that is, recapitulation of tumor phenotypes in constructed organoids. Here, we report a method of microfluidic droplet encapsulation that provides structural guidance for tumor cell growth and organization, where they develop into tumor organoids with high purity and high rates of modeling success, as compared to the classical organoid modeling method, that is, non-engineered organoids.

Light-driven MOF-based micromotors with self-floating characteristics for water sterilization.

Three-dimensional motion (especially in the -axis direction) of metal-organic frameworks (MOFs)-based micromotors (MOFtors) is essential but still in its infancy. Herein, we propose a simple strategy for designing light-driven MOFtors that move in the -axis direction and efficiently kill (). The as-prepared polypyrrole nanoparticles (PPy NPs) with excellent photothermal properties are combined with ZIF-8 through a simple encapsulation method, resulting in multi-wavelength photothermally-responsive MOFtors (PPy/ZIF-8).

The uncovered biases and errors in clinical determination of bone age by using deep learning models.

Objectives: To evaluate AI biases and errors in estimating bone age (BA) by comparing AI and radiologists' clinical determinations of BA.

Methods: We established three deep learning models from a Chinese private dataset (CHNm), an American public dataset (USAm), and a joint dataset combining the above two datasets (JOIm). The test data CHNt (n = 1246) were labeled by ten senior pediatric radiologists.

Large-Scale Self-Assembly of MOFs Colloidosomes for Bubble-Propelled Micromotors and Stirring-Free Environmental Remediation.

The design of MOF-based micromotors (MOFtors) is still challenging and with limited approaches, especially for the MOF nanoparticles (NPs). Herein, we report a universal and straightforward strategy to efficiently self-assembly MOF NPs into robust MOFtors for enhanced organic- or heavy-metal-ion-contaminants remediation without mechanical stirring. Based on the transient Pickering emulsion method, Fe O @NH -UiO-66 (Fe-UiO) NPs are rapidly self-assembled into Fe O @NH -UiO-66 colloidosomes (Fe-UiOSomes) on a large scale, and the formation mechanism is systematically studied.

Droplet-engineered organoids recapitulate parental tissue transcriptome with inter-organoid homogeneity and inter-tumor cell heterogeneity.

Organoids are expected to function as effective human organ models for precision cancer studies and drug development. Currently, primary tissue-derived organoids, termed non-engineered organoids (NEOs), are produced by manual pipetting or liquid handling that compromises organoid-organoid homogeneity and organoid-tissue consistency. Droplet-based microfluidics enables automated organoid production with high organoid-organoid homogeneity, organoid-tissue consistency, and a significantly improved production spectrum.

Laser-based bioprinting for multilayer cell patterning in tissue engineering and cancer research.

3D printing, or additive manufacturing, is a process for patterning functional materials based on the digital 3D model. A bioink that contains cells, growth factors, and biomaterials are utilized for assisting cells to develop into tissues and organs. As a promising technique in regenerative medicine, many kinds of bioprinting platforms have been utilized, including extrusion-based bioprinting, inkjet bioprinting, and laser-based bioprinting.

Amplitude of low-frequency fluctuation may be an early predictor of delayed motor development due to neonatal hyperbilirubinemia: a fMRI study.

Background: Acute bilirubin encephalopathy or kernicterus is the worst consequence of brain damage caused by the elevation of total unbound serum bilirubin (TSB) in neonates. The present study aimed to visualize the characteristic brain regions of neonates with hyperbilirubinemia (HB) using functional magnetic resonance imaging (fMRI) and to measure the amplitude of low-frequency fluctuation (ALFF) values.

Methods: This was a prospective cohort study, which included newborns with HB who were hospitalized at the Children's Hospital of Fudan University.

Altered striatum centered brain structures in SHANK3 deficient Chinese children with genotype and phenotype profiling.

SHANK3 deficiency represents one of the most replicated monogenic risk factors for autism spectrum disorder (ASD) and SHANK3 caused ASD presents a unique opportunity to understand the underlying neuropathological mechanisms of ASD. In this study, genetic tests, comprehensive clinical and neurobehavioral evaluations, as well as multimodal structural MRI using voxel-based morphometry (VBM) and tract-based spatial statistics (TBSS) were conducted in SHANK3 group (N = 14 with SHANK3 defects), ASD controls (N = 26 with idiopathic ASD without SHANK3 defects) and typically developing (TD) controls (N = 32). Phenotypically, we reported several new features in Chinese SHANK3 deficient children including anteverted nares, sensory stimulation seeking, dental abnormalities and hematological problems.

CoO@CdS Hollow Spheres Derived from ZIF-67 with a High Phenol and Dye Photodegradation Activity.

The CoO@CdS double-layered hollow spheres were first prepared by the template-removal method with the assistance of the ZIF-67 material; the structure has been proved by transmission electron microscopy (TEM). The CoO@CdS hollow spheres calcinated at 400 °C exhibited the highest photodegradation activity. Nearly 90% phenol was degraded after 2 h of visible-light irradiation.

Application of Robot-Assisted Frameless Stereoelectroencephalography Based on Multimodal Image Guidance in Pediatric Refractory Epilepsy: Experience of a Pediatric Center in a Developing Country.

Objective: To introduce the application of robot-assisted frameless stereoelectroencephalography (SEEG) based on multimodal image fusion technology in pediatric refractory epilepsy in a pediatric center from a developing country.

Methods: We retrospectively evaluated pediatric patients with drug-resistant epilepsy who underwent SEEG monitoring at the Children's Hospital of Fudan University from July 2014 to August 2017. Application of multimodal image fusion technology in SEEG was described in detail.

DeepVolume: Brain Structure and Spatial Connection-Aware Network for Brain MRI Super-Resolution.

Thin-section magnetic resonance imaging (MRI) can provide higher resolution anatomical structures and more precise clinical information than thick-section images. However, thin-section MRI is not always available due to the imaging cost issue. In multicenter retrospective studies, a large number of data are often in thick-section manner with different section thickness.

Clinical Features, Treatment, and Outcomes Among Chinese Children With Anti-methyl-D-aspartate Receptor (Anti-NMDAR) Encephalitis.

Anti-N-methyl-D-aspartate receptor (anti-NMDAR) encephalitis is the most common form of autoimmune encephalitis in pediatric patients. In this study, we aimed to investigate the clinical features and long-term outcomes of pediatric patients with anti-NMDAR encephalitis in China. We conducted a retrospective study of children (age range: 0-18 years) with anti-NMDAR encephalitis treated at Children's Hospital of Fudan University between July 2015 and November 2018.

Clinical features of postinfectious bronchiolitis obliterans in children undergoing long-term nebulization treatment.

Background: Limited data are available in relation to the clinical features of PIBO undergoing prolonged nebulization treatment with budesonide, terbutaline and ipratropium bromide. This retrospective study aimed to outline the features of clinical, high-resolution computed tomography (HRCT) and pulmonary function test (PFT) of PIBO, undergoing maintenance therapy utilizing a triple nebulization treatment and to determine the factors associated with prognosis.

Methods: Children diagnosed with PIBO were followed up between April 2014 and March 2017.