The Mediating Effect of Psychological Flexibility in the Relationship Between Two-Way Social Support and Rehabilitation Exercise Adherence in Patients With Total Knee Arthroplasty.

Objective: This study aims to investigate the impact of two-way social support on rehabilitation exercise compliance among patients undergoing total knee arthroplasty and examine psychological flexibility's mediating role.

Design: Cross-sectional study.

Methods: A convenience sample of 266 total knee arthroplasty patients was recruited from the orthopaedic department of a tertiary hospital in Guiyang, Guizhou Province, between November 12, 2024, and March 20, 2025.

Technical innovations of signal amplification in fluorescence in situ hybridization.

The in situ hybridization approach is used to detect nucleotide sequences in cells, tissue slices, as well as even three-dimensional organs and organisms. This approach is based on the complementary binding of nucleotide probes and unique DNA or RNA target sequences. These probes can be labeled with fluorescent, radioactive or antigen molecules.

Prenatal Exome Sequencing for Fetal Macrocephaly: A Large Prospective Observational Cohort Study.

Objective: To assess the diagnostic utility of exome sequencing (ES) in macrocephalic fetuses.

Methods: Fetuses with macrocephaly (head circumference (HC) ≥ +2 SD) and negative chromosomal microarray results were included, who had available trio-ES data. Molecular diagnoses were systematically analyzed.

Association Between Electrical Conductivity and Global Impedance Measured by EIT with Muscle Mass Scores in Sarcopenia.

Electrical impedance tomography (EIT) imaging is used for quantitative assessment of muscle mass and function in elderly with sarcopenia. However, the differences in EIT-derived parameters between patients with and without sarcopenia and the correlation between parameters and muscle function scores are unclear. Thirty-one sarcopenic and healthy subjects were recruited in this work, and maximal handgrip strength (MHG), maximal isometric voluntary contraction (MVC), and SARC-F scale of all individuals were used for muscle function scoring.

Cortico-muscular Information Transfer Based on fNIRS and sEMG During Gait Walking in the Elderly and Young Adults.

Decreased gait ability occurs in the aging process, increasing the risk of falls and reducing quality of life. Gait control relies on cortical and muscle activation and cortico-muscular information transmission, but the impact of aging on cortical and muscle information transmission during gait is currently unclear. This study recruited 12 young adults and 12 elderly subjects, and simultaneously acquired cortical hemodynamic oxyhemoglobin concentration (HbO) and gastrocnemius surface electromyography (sEMG) information during walking, and based on transfer entropy (TE) to estimate cortico-muscular information transfer.

Quantitative Analysis of the Gait Variability and Asymmetry Using Inertial Measurement Unit During Dual-task Gait.

Well-established connections exist between increased gait variability and greater fall likelihood in human movement. Therefore, the assessment of gait symmetry can facilitate clinicians' decision-making in the treatment and evaluation of the progression of several diseases. This study proposed a workflow that used two self-developed inertial sensing measurement units (IMU) to collect the lower limb gait data of five subjects and then extracted the gait characteristic parameters to quantify the running process during a single-task condition and a dual-task condition, which requires additional cognitive loads (counting numbers backward).

The essential role of sphingolipids in TRPC5 ion channel localization and functionality within lipid rafts.

Sphingolipids are critical components of cellular membranes that play a pivotal role in modulating ion channel function by forming lipid rafts that stabilize and localize these channels. These lipids regulate membrane fluidity and protein-lipid interactions, directly influencing ion channel activity, trafficking, and signaling pathways essential for maintaining cellular homeostasis. Despite their fundamental role, the impact of sphingolipids on ion channel functionality, particularly within the nervous system, remains insufficiently understood.

A novel multi-level 3D pose estimation framework for gait detection of Parkinson's disease using monocular video.

Introduction: Parkinson's disease (PD) is characterized by muscle stiffness, bradykinesia, and balance disorders, significantly impairing the quality of life for affected patients. While motion pose estimation and gait analysis can aid in early diagnosis and timely intervention, clinical practice currently lacks objective and accurate tools for gait analysis.

Methods: This study proposes a multi-level 3D pose estimation framework for PD patients, integrating monocular video with Transformer and Graph Convolutional Network (GCN) techniques.

First Reported Case of Hemoglobin H Disease Caused by the Rare α-Globin Gene Mutation ( c.244delT) in a Chinese Family.

Microcytosis of red cells and mild anemia are common in thalassemia carriers but those phenotypes are not specific. It is really a challenge for clinical interpretation of those variants. Co-segregation with disease in affected family members or specific phenotypes such as the abnormal Hb H are very helpful to assess the pathogenicity of rare variants.

MPV17 Prevents Myocardial Ferroptosis and Ischemic Cardiac Injury through Maintaining SLC25A10-Mediated Mitochondrial Glutathione Import.

Ferroptosis is a recently identified iron-dependent programmed cell death with lipid peroxide accumulation and condensation and compaction of mitochondria. A recent study indicated that ferroptosis plays a pivotal role in ischemic cardiac injury with the mechanisms remain largely unknown. This study demonstrates that when an iron overload occurs in the ischemia/reperfusion cardiac tissues, which initiates myocardial ferroptosis, the expression levels of mitochondrial inner membrane protein MPV17 are reduced.

[MPV17 inhibits iron overload-induced ferroptosis of splenic CD3 T cells in mice by blocking ERK pathway].

Objective This work aimed to explore the effect of iron overload on splenic injury and the role of MPV17 in the ferroptosis of splenic CD3 T cells from mice subjected to iron overload. Methods Mice were randomly divided into normal diet group, high-iron diet group, high-iron diet combined with Fer-1 treatment group, and high-iron diet combined with adenovirus harboring MPV17 injection group, with 5 mice in each group. After treatment for 8 weeks, mice spleens were harvested and fixed; Histological section and HE staining were performed to observe the structures of the spleens; Cell death of CD3 T cells was detected by propidium iodide (PI) staining; The lipid peroxidation levels were detected by C11 BODIPY581/591 staining; The mRNA levels of Solute carrier family 7 member 11 (SLC7A11) and prostaglandin-endoperoxide synthase 2 (PTGS2) were detected by qPCR assays; The macrophage phenotype-switching (M1/M2) were detected by flow cytometry; The levels of TNF-α, IL-1β and IL-6 were measured by ELISA assays.

Variant spectrum of and in hemophilia patients from southern China and 26 novel variants.

Hemophilia, an X-linked recessive disorder, is characterized by spontaneous or trauma-induced prolonged bleeding. It is classified as hemophilia A when caused by variants in the gene, and hemophilia B when caused by variants. Few studies have described hemophilia variants in the Chinese population.

Iprodione induces hepatotoxicity in zebrafish by mediating ROS generation and upregulating p53 signalling pathway.

Iprodione is an effective and broad-spectrum fungicide commonly used for early disease control in fruit trees and vegetables. Due to rainfall, iprodione often finds its way into water bodies, posing toxicity risks to non-target organisms and potentially entering the human food chain. However, there is limited information available regarding the developmental toxicity of iprodione specifically on the liver in existing literature.

Mechanosensitive channels TMEM63A and TMEM63B mediate lung inflation-induced surfactant secretion.

Pulmonary surfactant is a lipoprotein complex lining the alveolar surface to decrease the surface tension and facilitate inspiration. Surfactant deficiency is often seen in premature infants and in children and adults with respiratory distress syndrome. Mechanical stretch of alveolar type 2 epithelial (AT2) cells during lung expansion is the primary physiological factor that stimulates surfactant secretion; however, it is unclear whether there is a mechanosensor dedicated to this process.

TMEM63 mechanosensitive ion channels: Activation mechanisms, biological functions and human genetic disorders.

The transmembrane 63 (TMEM63) family of proteins are originally identified as homologs of the osmosensitive calcium-permeable (OSCA) channels in plants. Mechanosensitivity of OSCA and TMEM63 proteins are recently demonstrated in addition to their proposed activation mechanism by hyper/hypo-osmolarity. TMEM63 proteins exist in all animals, with a single member in Drosophila (TMEM63) and three members in mammals (TMEM63 A/B/C).

To Be or Not to Be an Ion Channel: Cryo-EM Structures Have a Say.

Ion channels are the second largest class of drug targets after G protein-coupled receptors. In addition to well-recognized ones like voltage-gated Na/K/Ca channels in the heart and neurons, novel ion channels are continuously discovered in both excitable and non-excitable cells and demonstrated to play important roles in many physiological processes and diseases such as developmental disorders, neurodegenerative diseases, and cancer. However, in the field of ion channel discovery, there are an unignorable number of published studies that are unsolid and misleading.

Ca Influx through TRPC Channels Is Regulated by Homocysteine-Copper Complexes.

An elevated level of circulating homocysteine (Hcy) has been regarded as an independent risk factor for cardiovascular disease; however, the clinical benefit of Hcy lowering-therapy is not satisfying. To explore potential unrevealed mechanisms, we investigated the roles of Ca influx through TRPC channels and regulation by Hcy-copper complexes. Using primary cultured human aortic endothelial cells and HEK-293 T-REx cells with inducible TRPC gene expression, we found that Hcy increased the Ca influx in vascular endothelial cells through the activation of TRPC4 and TRPC5.