Single-cell transcriptomic atlas of blood and lung from mice infected with SARS-CoV-2 revealing distinct virulence characteristics between prototype and Omicron BA.1 strain.

The markedly reduced pathogenicity of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Omicron variant in comparison to earlier strains has raised critical questions regarding its underlying mechanisms. To elucidate the host immune responses driving these differences, we performed single-cell transcriptomic profiling of lung and blood samples from human angiotensin-converting enzyme 2 (hACE2) transgenic mice infected with either the SARS-CoV-2 prototype strain or the Omicron BA.1 variant at 5 days post-inoculation.

Dual-Functional Antimicrobial and Anticoagulant Coatings: Synergistic Mechanisms, Research Advances, and Translational Challenges.

The adhesion of biological components such as bacteria, proteins, and platelets on material surfaces triggers biofilm formation and thrombus generation, which leads to severe infections and embolism risks in blood-contacting medical devices. Conventional blood-contacting coatings predominantly focus on singular antibacterial or anticoagulant functions, while clinical demands urgently require surfaces with synergistic "antibacterial-anticoagulant" efficacy to address complex biofouling challenges. Recent advancements in dual-functional antibacterial-anticoagulant coatings have achieved notable progress.

Immune imprinting blunts omicron pathogenicity fluctuations and highlights the essential role of cellular immunity in SARS-CoV-2 infection.

The emergence of Omicron variants challenged early SARS-CoV-2 vaccination and infection-induced immunity, leading to frequent breakthrough infections. While Omicron is generally considered less pathogenic, our retrospective clinical data and multi-species animal models revealed that subvariants BF.7 and XBB.

Ultrasound-Assisted for Super-Rapid and High-Efficient Adsorption and Desorption.

Adsorption-desorption technology is the basis of numerous environmental processes and industries in modern society. Undoubtedly, improving adsorption rates and renewability of sorbents can positively impact productivity. It is herein showed that poly(vinylidene fluoride-co-hexafluoropropylene) (PVDF-HFP) presented remarkable adsorption kinetics and regeneration efficiency under ultrasound.

Dynamic Molecular Changes in Brain, Lung, and Heart of Hamsters Infected With SARS-CoV-2: Insights From a Severe and Recovery Phase Model.

The Global pandemic of coronavirus disease 2019 was initiated by the emergence of severe acute respiratory syndrome coronavirus 2. In addition to conventional pulmonary lesions, a range of neurological injury symptoms have been identified in clinical practice, but the aetiology of neurological disorders linked to SARS-CoV-2 infection remains poorly understood. Syrian hamsters, which are highly susceptible to SARS-CoV-2 infection, exhibit a disease phenotype similar to that observed in human COVID-19 patients.

Research on Collision Access Method for Satellite Internet of Things Based on Bayliss Window Function.

Satellite Internet of Things (IoT) terminals face design constraints regarding low power consumption and light control. These constraints pose a significant collision risk when utilizing traditional random-access protocols, making it challenging to meet the system throughput requirements. Auxiliary beam schemes based on conventional beam formation suffer from the problem of the auxiliary beam shape being limited by the fixed directional map.

Seasonality and Vertical Structure of Microbial Communities in Alpine Wetlands.

The soil microbial community plays a crucial role in the elemental cycling and energy flow within wetland ecosystems. The temporal dynamics and spatial distribution of soil microbial communities are central topics in ecology. While numerous studies have focused on wetland microbial community structures at low altitudes, microbial diversity across seasons and depths and their environmental determinants remain poorly understudied.

Hierarchical Resource Management for Mega-LEO Satellite Constellation.

The mega-low Earth orbit (LEO) satellite constellation is pivotal for the future of satellite Internet and 6G networks. In the mega-LEO satellite constellation system (MLSCS), which is the spatial distribution of satellites, global users, and their services, along with the utilization of global spectrum resources, significantly impacts resource allocation and scheduling. This paper addresses the challenge of effectively allocating system resources based on service and resource distribution, particularly in hotspot areas where user demand is concentrated, to enhance resource utilization efficiency.

The Development of Animal Models for Respiratory Syncytial Virus (RSV) Infection and Enhanced RSV Disease.

The development of immunoprophylactic products against respiratory syncytial virus (RSV) has resulted in notable advancements, leading to an increased demand for preclinical experiments and placing greater demands on animal models. Nevertheless, the field of RSV research continues to face the challenge of a lack of ideal animal models. Despite the demonstration of efficacy in animal studies, numerous RSV vaccine candidates have been unsuccessful in clinical trials, primarily due to the lack of suitable animal models.

Rapid virus inactivation by nanoparticles-embedded photodynamic surfaces.

The persistent threat of viral epidemics poses significant risks to human health, highlighting the urgent need for antiviral surfaces to mitigate viral transmission through bioaerosols and surface contamination. However, there is still a scarcity of readily accessible antiviral coatings to address this critical concern. In this study, we demonstrate that photodynamic nanoparticle-embedded surfaces can swiftly inactivate both enveloped and non-enveloped viruses.

Predictive factors for intrathoracic anastomotic leakage and postoperative mortality after esophageal cancer resection.

Background: Esophageal cancer is currently one of the high-risk malignant tumors worldwide, posing a serious threat to human health. This study aimed to analyse the causes of postoperative mortality and intrathoracic anastomotic leakage(IAL) after esophagectomy.

Methods: A retrospective analysis was conducted on 172 patients with esophageal cancer resection and focused on the preoperative and postoperative indicators.

Elevational distribution patterns and drivers factors of fungal community diversity at different soil depths in the var. smithii forests on Sygera Mountains, southeastern Tibet, China.

Introduction: Soil fungal communities play a crucial role in maintaining the ecological functions of alpine forest soil ecosystems. However, it is currently unclear how the distribution patterns of fungal communities in different soil layers of alpine forests will change along the elevational gradients.

Material And Methods: Therefore, Illumina MiSeq sequencing technology was employed to investigate fungal communities in three soil layers (0-10, 10-20, and 20-30 cm) along an elevational gradient (3500 m to 4300 m) at Sygera Mountains, located in Bayi District, Nyingchi City, Tibet.

Review of energy self-circulation systems integrating biogas utilization with Powerfuels production in global livestock industry.

Energy self-circulation systems, defined as energy systems incorporating the recycling utilization of waste biomass, have been proposed to reduce greenhouse gases emissions from livestock sector. In this study, a comprehensive review of the situation and challenges of biogas utilization in the livestock industry was provided. Moreover, two technological routes were proposed for a circular livestock system combined with Powerfuels production (CP-CLS), starting from biogas combustion for power generation and steam reforming to the sustainable development path of synthesizing, storing, and utilizing Powerfuels.

The Milk of Cows Immunized with Trivalent Inactivated Vaccines Provides Broad-Spectrum Passive Protection against Hand, Foot, and Mouth Disease in Neonatal Mice.

Hand, foot, and mouth disease (HFMD) is a contagious viral infection predominantly affecting infants and young children, caused by multiple enteroviruses, including Enterovirus 71 (EV71), Coxsackievirus A16 (CA16), Coxsackievirus A10 (CA10), and Coxsackievirus A6 (CA6). The high pathogenicity of HFMD has garnered significant attention. Currently, there is no specific treatment or broad-spectrum preventive measure available for HFMD, and existing monovalent vaccines have limited impact on the overall incidence or prevalence of the disease.

Ba-doping introduced piezoelectricity and efficient Ultrasound-Triggered bactericidal activity of brookite TiO nanorods.

Exploring highly efficient ultrasound-triggered catalysts is pivotal for various areas. Herein, we presented that Ba doped brookite TiO nanorod (TiO: Ba) with polarization-induced charge separation is a candidate. The replacement of Ba for Ti not only induced significant lattice distortion to induce polarization but also created oxygen vacancy defects for facilitating the charge separation, leading to high-efficiency reactive oxygen species (ROS) evolution in the piezo-catalytic processes.

Comparison of the pathogenicity and neutrophil and monocyte response between SARS-CoV-2 prototype and Omicron BA.1 in a lethal mouse model.

Background: SARS-CoV-2, first identified in late 2019, has given rise to numerous variants of concern (VOCs), posing a significant threat to human health. The emergence of Omicron BA.1.

An Indocyanine Green-Based Nanocluster for Imaging Orthodox Endometriosis Lesions with Negative Contrast.

Indocyanine green (ICG), as the sole near-infrared dye FDA-approved, is limited in biomedical applications because of its poor photostability, lack of targeting, and rapid removal in vivo. Herein, we presented a nanoformulation of poly-l-lysine-indocyanine green-hyaluronic acid (PIH) and demonstrated that it can image orthodox endometriosis (EM) lesions with a negative contrast. The PIH nanocluster, with an average diameter of approximately 200 nm, exhibited improved fluorescence photostability and antioxidant ability compared to free ICG.

Narrowband Internet of Things via Low Earth Orbit Satellite Networks: An Efficient Coverage Enhancement Mechanism Based on Stochastic Geometry Approach.

With the development of IoT technology and 5G massive machine-type communication, the 3GPP standardization body considered as viable the integration of Narrowband Internet of Things (NB-IoT) in low Earth orbit (LEO) satellite-based architectures. However, the presence of the LEO satellite channel comes up with new challenges for the NB-IoT random access procedures and coverage enhancement mechanism. In this paper, an Adaptive Coverage Enhancement (ACE) method is proposed to meet the requirement of random access parameter configurations for diverse applications.

Pathogenicity and landscape of differential gene expression in mice orally infected with clinical coxsackievirus A6 (CA6).

Hand, foot, and mouth disease (HFMD) is caused by more than 20 pathogenic enteroviruses belonging to the Picornaviridae family and genus. Since the introduction of the enterovirus-71 (EV71) vaccine in 2016, the number of HFMD cases caused by EV71 has decreased. However, cases of infections caused by other enteroviruses, such as coxsackievirus A6 (CA6) and coxsackievirus A10, have been increasing accordingly.

Nighttime Image Stitching Method Based on Image Decomposition Enhancement.

Image stitching technology realizes alignment and fusion of a series of images with common pixel areas taken from different viewpoints of the same scene to produce a wide field of view panoramic image with natural structure. The night environment is one of the important scenes of human life, and the night image stitching technology has more urgent practical significance in the fields of security monitoring and intelligent driving at night. Due to the influence of artificial light sources at night, the brightness of the image is unevenly distributed and there are a large number of dark light areas, but often these dark light areas have rich structural information.

Networks of mineral-associated organic matter fractions in forest ecosystems.

Mineral-associated organic matter (MAOM), the largest soil carbon pool, is formed through a series of organo-mineral interaction mechanisms. However, different organo-mineral fractions relevant to specific stabilization mechanisms and their response to environmental variables are poorly understood, which hinders accurate prediction of MAOM preservation under climate change. We applied sequential chemical extraction to separate MAOM into different organo-mineral fractions.

pH: A core node of interaction networks among soil organo-mineral fractions.

Mineral-associated organic matter (MAOM) is the largest soil organic carbon (OC) pool with the longest turnover. MAOM is expected to have relatively little sensitivity to climate change due to mineral protection, but its persistence involves several organo-mineral fractions. The uncertainty in the response of specific organo-mineral fractions to climate change hampers the reliability of predictions of MAOM preservation in the future.

An Asynchronous Collision-Tolerant ACRDA Scheme Based on Satellite-Selection Collaboration-Beamforming for LEO Satellite IoT Networks.

In this paper, an asynchronous collision-tolerant ACRDA scheme based on satellite-selection collaboration-beamforming (SC-ACRDA) is proposed to solve the avalanche effect caused by packet collision under random access (RA) high load in the low earth orbit (LEO) satellite Internet of Things (IoT) networks. A non-convex optimization problem is formulated to realize the satellite selection problem in multi-satellite collaboration-beamforming. To solve this problem, we employ the Charnes-Cooper transformation to transform a convex optimization problem.

Effects of Different Nanoparticles on Microbes.

Nanoparticles widely exist in nature and may be formed through inorganic or organic pathways, exhibiting unique physical and chemical properties different from those of bulk materials. However, little is known about the potential consequences of nanomaterials on microbes in natural environments. Herein, we investigated the interactions between microbes and nanoparticles by performing experiments on the inhibition effects of gold, ludox and laponite nanoparticles on in liquid Luria-Bertani (LB) medium at different nanoparticle concentrations.

Indoor Positioning on Smartphones Using Built-In Sensors and Visual Images.

With the rapid development of mobile Internet technology, localization using visual image information has become a hot problem in the field of indoor localization research, which is not affected by signal multipath and fading and can achieve high accuracy localization in indoor areas with complex electromagnetic environments. However, in practical applications, position estimation using visual images is easily influenced by the user's photo pose. In this paper, we propose a multiple-sensor-assisted visual localization method in which the method constructs a machine learning classifier using multiple smart sensors for pedestrian pose estimation, which improves the retrieval efficiency and localization accuracy.