Elastin-like polypeptide and triclosan-modified PCL membrane provides aseptic protection in tissue regeneration.

Tissue regeneration is a spatiotemporally ordered multidimensional process involving hierarchical structures, vascularization, and metabolic-immune properties, while injury often triggers structural disorganization, inflammation, metabolic dysfunction, and mechanical impairment. Based on these findings, we designed a functionalized polycaprolactone (PCL) porous scaffold loaded with biocompatible elastin and the antibacterial drug triclosan, which specifically inhibits the growth of methicillin-resistant (MRSA). Using a bio-mimetic mussel adhesion approach, a polydopamine coating was formed on the surface of electrospun PCL membranes to provide grafting sites for elastin-like polypeptides (ELP), attaching elastin peptides and triclosan to the PCL surface.

Dual-Responsive Fluorescent Sensor for Monitoring ALP and SO in Cholestatic Liver Injury Evaluation and Drug Screening.

Cholestatic liver injury (CLI) is a pathological condition characterized by impaired bile flow and elevated levels of oxidative stress. Its clinical manifestations are often asymptomatic in the early stage, while prolonged CLI may progress to liver failure without timely intervention. However, current diagnostic and therapeutic approaches for CLI are limited in both effectiveness and availability, highlighting the critical need for reliable diagnostic modalities and innovative therapeutic agents.

Upregulation of Protein O-GlcNAcylation Levels Promotes Zebrafish Fin Regeneration.

As one of the most important posttranslational modifications, glycosylation participates in various cellular activities in organisms and is closely associated with many pathogeneses. It has been reported that glycosylation affects the liver, spinal cord, and heart tissue regeneration. The zebrafish fin has become a valuable model due to its high regenerative capacity.

A bilayer scaffold of collagen and nanohydroxyapatite promotes osteochondral defect in rabbit knee joints.

Aims: A large number of surgical operations are available to treat osteochondral defects of the knee. However, the knee joint arthroplasty materials cannot completely mimic the articular cartilage and subchondral bone, which may bring some obvious side effects. Thus, this study proposed a biocompatible osteochondral repair material prepared from a double-layer scaffold of collagen and nanohydroxyapatite (CHA), consisting of collagen hydrogel as the upper layer of the scaffold, and the composite of CHA as the lower layer of the scaffold.

DDX17-Mediated Upregulation of CXCL8 Promotes Hepatocellular Carcinoma Progression Co-activating β-catenin/NF-κB Complex.

Hepatocellular carcinoma (HCC) is a well-known inflammation-related cancer, that accounts for fifth most prevalent neoplasm and the third major driver of cancer associated fatality globally. Accumulating evidence has elucidated that C-X-C motif chemokine ligands (CXCLs) are aberrantly upregulated in HCC and are involved in inflammation-induced hepatocarcinogenesis and metastasis. Herein, we identified a novel function of DEAD-box RNA helicase 17 (DDX17) as an oncogenic factor via transactivating CXCL8 in HCC.

Development of 17-4 PH Stainless Steel for Low-Power Selective Laser Sintering.

Selective laser sintering (SLS) is one of the prominent methods of polymer additive manufacturing (AM). A low-power laser source is used to directly melt and sinter polymer material into the desired shape. This study focuses on the utilization of the low-power laser SLS system to successfully manufacture metallic components through the development of a metal-polymer composite material.

A Recombinant Human Collagen and RADA-16 Fusion Protein Promotes Hemostasis and Rapid Wound Healing.

In this study, we designed a fusion protein, rhCR, by combining human collagen with the self-assembling peptide RADA-16 using genetic engineering technology. The rhCR protein was successfully expressed in . The rhCR can self-assemble into a three-dimensional nanofiber network under physiological conditions.

FoxO is required for neoblast differentiation during planarian regeneration.

Stem cells are of great importance in the maintenance and regeneration of tissues, with Forkhead box O (FoxO) proteins emerging as pivotal regulators of their functions. However, the precise impact of FoxO proteins on stem cell behavior within regenerative environments remains ambiguous. Planarians, renowned for their abundance of adult stem cells (neoblasts), serve as an excellent model for investigating the dynamics of stem cells during regeneration.

The association between chronotype and social anxiety among Chinese university students: a moderated mediation analysis of loneliness and perceived social support.

Background: Social anxiety has been a burning problem among contemporary college students in China. Increasing evidence suggests that individual circadian typology-chronotype may play an important role in the development of social anxiety. However, little research has focused directly on examining the association between chronotype and social anxiety, and less is known about the mediating and moderating mechanisms underlying this relationship.

Circadian rhythm regulates the function of immune cells and participates in the development of tumors.

Circadian rhythms are present in almost all cells and play a crucial role in regulating various biological processes. Maintaining a stable circadian rhythm is essential for overall health. Disruption of this rhythm can alter the expression of clock genes and cancer-related genes, and affect many metabolic pathways and factors, thereby affecting the function of the immune system and contributing to the occurrence and progression of tumors.

Periodic static compression of micro-strain pattern regulates endochondral bone formation.

Developmental engineering based on endochondral ossification has been proposed as a potential strategy for repairing of critical bone defects. Bone development is driven by growth plate-mediated endochondral ossification. Under physiological conditions, growth plate chondrocytes undergo compressive forces characterized by micro-mechanics, but the regulatory effect of micro-mechanical loading on endochondral bone formation has not been investigated.

Forkhead box O proteins: steering the course of stem cell fate.

Stem cells are pivotal players in the intricate dance of embryonic development, tissue maintenance, and regeneration. Their behavior is delicately balanced between maintaining their pluripotency and differentiating as needed. Disruptions in this balance can lead to a spectrum of diseases, underscoring the importance of unraveling the complex molecular mechanisms that govern stem cell fate.

Facile synthesis of a rod-like Ni/TiO/C nanocomposite for enhanced electromagnetic wave absorption.

In this study, we utilized a simple calcination method to prepare a Ni/TiO/C composite, which was synchronously grown from magnetic, semiconductor, and conductive materials. XRD, SEM, Raman, and XPS characterization methods were used to analyze the crystal structure, graphitization degree, morphology size, and valence state of Ni/TiO/C, and its electromagnetic wave absorption performance was tested. It was revealed that rod-like Ni/TiO/C had good electromagnetic wave absorption performance at a thickness of 1-5.

High-sensitivity humidity and temperature sensor by cascading a polyimide coated long period fiber grating and a polydimethylsiloxane wrapped Mach-Zehnder interferometer.

Two different structures, long period fiber grating (LPFG) and a Mach-Zehnder interferometer (MZI), were cascaded to fabricate a sensor for sensing humidity and temperature simultaneously. Due to the humidity sensitive characteristic of polyimide (PI) and the temperature sensitive characteristic of polydimethylsiloxane (PDMS), the LPFG was coated with PI and the MZI was wrapped in PDMS to improve the humidity and temperature sensitivities, respectively. Humidity and temperature experiments and the stability and repeatability of the proposed sensors were performed.

Highly sensitive magnetic field sensors based on FeO nanorods grown on the surface of a tapered few mode fiber.

A magnetic field (MF) sensor with a stable structure and high sensitivity has been proposed and experimentally verified. We used the water bath method to produce a layer of FeO nanorods on a tapered few mode fiber (FMF) surface to form a Mach-Zehnder interferometer (MZI). The experiment found that the nanostructure produced on the surface of FMF were particularly stable and firm.

Nano-Bio Interactions: Biofilm-Targeted Antibacterial Nanomaterials.

Biofilm is a spatially organized community formed by the accumulation of both microorganisms and their secretions, leading to persistent and chronic infections because of high resistance toward conventional antibiotics. In view of the tunable physicochemical properties and the related unique biological behavior (e.g.

The treatment of tuberculosis in the upper thoracic spine using the small incision technique through the third rib.

Background: The complex anatomical structure of the upper thoracic spine makes it challenging to achieve surgical exposure, resulting in significant surgical risks and difficulties. Posterior surgery alone fails to adequately address and reconstruct upper thoracic lesions due to limited exposure. While the anterior approach offers advantages in fully exposing the anterior thoracic lesions, the surgical procedure itself is highly intricate.

TATA-box-binding protein promotes hepatocellular carcinoma metastasis through epithelial-mesenchymal transition.

Background: HCC characterizes malignant metastasis with high incidence and recurrence. Thus, it is pivotal to discover the mechanisms of HCC metastasis. TATA-box-binding protein (TBP), a general transcriptional factor (TF), couples with activators and chromatin remodelers to sustain the transcriptional activity of target genes.

Redox Dysregulation in the Tumor Microenvironment Contributes to Cancer Metastasis.

Redox dysregulation under pathological conditions results in excessive reactive oxygen species (ROS) accumulation, leading to oxidative stress and cellular oxidative damage. ROS function as a double-edged sword to modulate various types of cancer development and survival. Emerging evidence has underlined that ROS impact the behavior of both cancer cells and tumor-associated stromal cells in the tumor microenvironment (TME), and these cells have developed complex systems to adapt to high ROS environments during cancer progression.

Impact of GTF2H1 and RAD54L2 polymorphisms on the risk of lung cancer in the Chinese Han population.

Background: Repair pathway genes play an important role in the development of lung cancer. The study aimed to assess the correlation between single nucleotide polymorphisms (SNPs) in DNA repair gene (GTF2H1 and RAD54L2) and the risk of lung cancer.

Methods: Five SNPs in GTF2H1 and four SNPs in RAD54L2 in 506 patients with lung cancer and 510 age-and gender-matched healthy controls were genotyped via the Agena MassARRAY platform.

Palladium-catalyzed oxidative Heck reaction of non-activated alkenes directed by fluorinated alcohol.

A novel, Pd-catalyzed oxidative Heck reaction of non-activated alkenes synergistically directed by bifunctional groups has been developed firstly by using O as a green oxidant, yielding the oxidative Heck products with excellent yields in a regio- and stereoselective manner. This bifunctional synergistic activation mechanism was demonstrated by experimental analysis and detailed computational studies, wherein the hydroxyl group directs the migratory insertion of the alkenes and the trifluoromethyl group facilitates the subsequent β-H elimination and reductive elimination. Moreover, a pesticidal active compound was synthesized using the bifunctional synergistically directed C-H arylation as the key step, which demonstrated its synthetic utility.

The Stimulation of Macrophages by Systematical Administration of GM-CSF Can Accelerate Adult Wound Healing Process.

Skin wound repair remains a major challenge in clinical care, and various strategies have been employed to improve the repair process. Recently, it has been reported that macrophages are important for the regeneration of various tissues and organs. However, their influence on wound repair is unclear.

Uniparental parthenogenetic embryonic stem cell derivatives adaptable for bone and cartilage regeneration.

Cells with the desired phenotype and number are critical for regenerative medicine and tissue engineering. Uniparental parthenogenetic embryonic stem cells (pESCs) share fundamental properties with embryonic stem cells. This study aims to determine the viability of pESC-based tissue engineering for bone and cartilage reconstruction.

Novel Fluorescence Probe toward Cu Based on Fluorescein Derivatives and Its Bioimaging in Cells.

Copper is an important trace element that plays a crucial role in various physiological and biochemical processes in the body. The level of copper content is significantly related to many diseases, so it is very important to establish effective and sensitive methods for copper detection in vitro and vivo. Copper-selective probes have attracted considerable interest in environmental testing and life-process research, but fewer investigations have focused on the luminescence mechanism and bioimaging for Cu detection.