RING-Between-RING-Type E3 Ligase Ariadne-Like Protein 8 Negatively Regulates Plant Virus Infection by Targeting a Viral Movement Protein.

The ubiquitin-proteasome system is a highly conserved machinery that plays a crucial role in plant defense against viruses. However, the number of E3 ligases targeting viral proteins remains limited. Although RING-between-RING (RBR)-type E3 ligases are evolutionarily conserved across organisms, their functions in plant responses to biotic stress remain largely unknown.

3D-Printed Extracellular Matrix-Mimicking Hydrogels Derived from Multiple Tissues for Integrated Construction of Complex Organs.

Due to the lack of suitable donors and concerns about immune rejection after transplantation, the demand for artificial organs among patients is increasing. Extracellular matrix-mimicking hydrogels provide excellent prospects for overcoming the limitations of current artificial organ construction methods. Here, a set of extracellular matrix-mimicking hydrogels derived from multiple animal tissues is described, which meets the regeneration needs of multiple tissues after xenotransplantation.

Huaier overcomes tumor-induced immunosuppression in colorectal cancer by activating MHC I and CD8+ T cells.

Background: Colorectal cancer (CRC) remains a leading cause of cancer-related mortality, primarily due to its immunosuppressive microenvironment that facilitates immune evasion and resistance to therapy. Although the traditional Chinese medicine Trametes robiniophila Murr (Huaier) has been shown to exert anti-tumor effects, its immunomodulatory potential in CRC and mechanistic interaction with immune checkpoints have not been investigated.

Results: Treatment with Huaier significantly improved overall survival in CRC patients (HR=0.

Prognostic and therapeutic implications related to glycosylation profiles of cancer-associated fibroblasts in colorectal cancer: insights from single-cell and bulk transcriptomics.

Glycosylation, a common post-translational modification of proteins, plays a role in numerous biological processes. However, the role of glycosylation in colorectal cancer (CRC) remains incompletely understood. In this study, we identified that CAFs exhibited the highest glycosylation levels in CRC.

Tumor-Stroma Ratio Is an Independent Prognostic Factor for Distant Metastasis in Squamous Cell Lung Cancer Following Resection.

Cancer distant metastasis is one of the main causes of cancer progression and difficulty in treatment (Rossi et al., 2020). This abstract aims to summarize the significance of tumor-stroma ratio (TSR) as a prognostic factor in the development of distant metastasis in squamous cell lung cancer (SQCLC) patients.

Addressing Challenges in Fluid Flow-Induced Cell Membrane Oscillation by Nanopipettes.

The mechanical properties of cell membranes are crucial to regulating cell morphology and behavior. Nanopipette-sensing has become distinctively appealing for the measurement of cell membrane mechanical properties due to its label-free operation and precise targeting. Recent progress has involved precise control of the movement and fluid flow of the nanopipet, which can induce cell membrane oscillation.

Nonlinear Optics in Low-Dimensional Nanomaterials.

Nonlinear optics describes the study of optical phenomena related to the interaction between intense light fields and matter [...

Reticulate allopolyploidy and subsequent dysploidy drive evolution and diversification in the cotton family.

Polyploidy and subsequent post-polyploid diploidization (PPD) are key drivers of plant genome evolution, yet their contributions to evolutionary success remain debated. Here, we analyze the Malvaceae family as an exemplary system for elucidating the evolutionary role of polyploidy and PPD in angiosperms, leveraging 11 high-quality chromosome-scale genomes from all nine subfamilies, including newly sequenced, near telomere-to-telomere assemblies from four of these subfamilies. Our findings reveal a complex reticulate paleoallopolyploidy history early in the diversification of the Malvadendrina clade, characterized by multiple rounds of species radiation punctuated by ancient allotetraploidization (Mal-β) and allodecaploidization (Mal-α) events around the Cretaceous-Paleogene (K-Pg) boundary.

Observation of Intrinsic and LED Light-Enhanced Memristor Performance in In-Plane Ferroelectric NbOI.

Two-dimensional (2D) layered ferroelectrics, as an emerging area of research, have attracted extensive attention, while memristors based on 2D ferroelectric materials are yet to be fully explored, thereby limiting their applications in modern nanoelectronics. In this work, we report the observation of intrinsic memristive behavior in a recently discovered 2D in-plane ferroelectric material, NbOI, and the giant enhancement of the memristive performance by using light-emission diode (LED) visible light. The results show that NbOI devices exhibit an intrinsically strong memristive response with a current on/off ratio of up to 10 and stable switching cycles, which is largely independent of the back-gate voltage.

Effects of active cycle of breathing techniques combined with external diaphragm pacing on respiratory function recovery in severe stroke patients with tracheotomy.

Background: Stroke causes a variety of dysfunctions, and thus the rehabilitation of such patients is important. The respiratory rehabilitation is necessary for stroke patients undergoing tracheotomy. The aim of this study was to provide evidences for a new rehabilitation program of the combination of active cycle of breathing technique (ACBT) with external diaphragm pacing (EDP) for the improvement of respiratory rehabilitation in severe stroke patients with tracheotomy.

Development and validation of a clinical risk prediction model for chronic sinusitis with nasal polyps: a retrospective analysis.

Object: This study aims to explore risk factors associated with chronic sinusitis with nasal polyps (CRSwNP) by analyzing the clinical pathophysiological factors in chronic sinusitis (CRS) patients.

Methods: General information of 206 CRS patients was collected along with Visual Analog Scale (VAS), snot score, Lund Kennedy and Lund Mackay assessments. Univariate analysis was performed to select variables for multivariate regression.

Ferroptosis-disulfidptosis-related CHMP6 is a clinico-immune target in colorectal cancer.

Background: Ferroptosis and disulfidptosis are newly discovered forms of regulated cell death that play critical roles in cancer progression, metabolism, and immune evasion. However, their interplay and combined influence on colorectal cancer (CRC) progression remain insufficiently understood.

Methods: We developed a ferroptosis-disulfidptosis-related gene (FDRG) score using machine-learning algorithms to analyze gene modifications associated with these pathways in CRC, utilizing data from the TCGA and GEO databases.

Inclined multilayer solar evaporator for directional and refined salt crystallization from practical industrial wastewater.

Interfacial solar evaporation with directional salt crystallization offers a sustainable approach for the mineral recovery. However, its application to real industrial wastewater is hindered by co-precipitate of organic pollutants, leading to crystal contamination. In this study, we present a rationally designed solar evaporator featuring dual functionality of directional crystallization and organic contaminants adsorption.

QTL detection and candidate gene identification for prostrate growth habit in interspecific crosses of wild chrysanthemum ( × ).

The prostrate growth habit is an important ornamental trait in ground-cover chrysanthemum, offering high aesthetic value, strong lodging resistance, and excellent landscape greening capability. However, the genetic basis underlying this trait in chrysanthemum remains largely unclear. In this study, we utilized the prostrate-type (tetraploid), the erect-type (tetraploid), and their 199 F hybrid progenies to construct a high-density genetic linkage map through genotyping-by-sequencing.

Predictive value of the pretreatment serum sialic acid/total protein ratio for bone metastases in newly diagnosed prostate cancer patients: development of a nomogram model.

Background: Bone is a common site of distant metastasis, and its metastasis is one of the major causes of death in prostate cancer (PCa) patients. Currently, there is a lack of effective predictive methods. This study aimed to evaluate the potential of pretreatment serum sialic acid/total protein (SA/TP) ratio as a biomarker for predicting PCa and its associated bone metastases.

AcABI5a integrates abscisic acid signaling to developmentally modulate fruit ascorbic acid biosynthesis in kiwifruit.

Consumers value highly the nutritional content and flavor of fresh fruits, which are influenced by endogenous plant hormones. However, the molecular mechanisms governing the hormonal regulation of essential nutrients such as ascorbic acid (AsA) in fruit are still unclear. This study investigates the regulation of AsA synthesis in kiwifruit by the transcription factor AcABI5a, which is involved in mediating the abscisic acid (ABA) signal.

Engineered Anti-Senescence Trachea With Post-Transplanted Regenerative Homeostasis.

Post-transplanted dysfunction remains the key unsolved challenge to construct bioengineered complex organs. Herein, by analyzing the bioengineered trachea, it is found that multiple tissue senescences occur, and poor endogenous cellular contact and exogenous immune microenvironment dysregulation are identified as two crucial factors during senescence. Therefore, a Hebe engineered trachea (Hebe-ET) with a ring-ring structure and dual anti-senescence designs is proposed for maintaining regenerative homeostasis after transplantation.

Natural variation of AcEGY3 mediates chloroplastic ROS homeostasis to confer kiwifruit thermotolerance.

The genus of Actinidia consists of the popular kiwifruits consuming worldwide. Most kiwifruit species are naturally distributed in warm and moist environments. So, they are vulnerable to high levels of heat stress.

Myelin oligodendrocyte glycoprotein antibody-associated cerebral cortical encephalitis: a case report highlighting diagnostic challenges and therapeutic implications.

Myelin oligodendrocyte glycoprotein (MOG) antibody-associated disease (MOGAD) encompasses a spectrum of inflammatory demyelinating disorders of the central nervous system (CNS). Recognized clinical phenotypes include optic neuritis (ON), transverse myelitis (TM), acute disseminated encephalomyelitis (ADEM), brainstem encephalitis, aseptic meningitis, cortical encephalitis, demyelinating pseudotumor, and cranial nerve involvement. MOG antibody-associated cerebral cortical encephalitis (MOG-CCE) represents a rare but clinically significant subtype, often misdiagnosed due to heterogeneous clinical and neuroimaging features overlapping with other CNS disorders.

Advances in understanding multifunctionality of Barley stripe mosaic virus γb protein.

Plant viruses usually encode versatile but limited number of proteins to reshape the cellular microenvironment, suppress or co-opt host pathways and proteins for their own benefit. Barley stripe mosaic virus (BSMV, Hordeivirus hordei) is a positive single-stranded RNA virus that infects both monocots and dicots. Among its seven encoded proteins, the γb protein exhibits remarkable multifunctionality despite being the smallest one.

Transgene- and tissue culture-free heritable genome editing using RNA virus-based delivery in wheat.

CRISPR-Cas genome editing technology is a cutting-edge strategy for crop breeding. However, the delivery of genome-editing reagents remains to be a technological bottleneck in monocot plants. Here we engineered barley yellow striate mosaic virus (BYSMV) into a negative-strand RNA virus-based vector system for delivery of both Cas9 and single guide RNA to achieve heritable gene editing in different wheat cultivars.

Nonlinear Imaging Detection of Organ Fibrosis in Minute Samples for Early Stage Utilizing Dual-Channel Two-Photon and Second-Harmonic Excitation.

Histopathological staining remains the fibrosis diagnostic gold standard yet suffers from staining artifacts and variability. Nonlinear optical techniques (e.g.

Plasmonic Nanoparticle Monolayer-Decorated Nanopipette Enabling in Situ Monitoring of Single-Cell Redox Metabolism.

The in-depth study of how single cells respond to redox perturbations is pivotal for predicting cell fate decisions. Surface-enhanced Raman spectroscopy (SERS)-active nanopipettes hold promise for single-cell analysis, yet challenges remain in achieving both signal sensitivity and reproducibility. Here, a plasmonic nanoparticle monolayer-decorated nanopipette has been developed, which serves as a biomimetic endoscope to monitor single-cell redox metabolism.