Mitochondrial Complex Member VdNuo1 Recruits Superoxide Dismutases VdSOD2/4 to Maintain Superoxide Anion Homeostasis During Pathogenesis in Verticillium dahliae.

During oxidative phosphorylation, the leaked electrons generate superoxide anions to attack the mitochondrial inner membrane and impair mitochondrial activity. Three superoxide dismutases (SODs) are secreted to degrade host superoxide anions in Verticillium dahliae. However, the roles of mitochondrial SODs (mtSODs) in superoxide anion detoxification and in virulence are unknown in this fungus.

Identification of In Vivo Components in Shiduqing Capsule Using High-Resolution Mass Spectrometry and Network Pharmacology-Based Analysis of Its Therapeutic Mechanisms Against Eczema.

Rationale: Shiduqing Capsule, a clinically widely used Chinese patent medicine, is indicated for skin itching, dryness, and desquamation caused by blood deficiency with wind-dryness syndrome. However, the tissue distribution characteristics of its active components in vivo and its pharmacological mechanisms in treating eczema remain unclear. This study aims to identify the bioactive components of Shiduqing Capsule, characterize their in vivo tissue distribution patterns, and subsequently elucidate the underlying therapeutic mechanisms against eczema based on these findings.

A ferroptosis-related risk model for non-coding RNA AC002331.1 in colon cancer: construction via competing endogenous RNA network analysis.

Background: Colon cancer, a globally prevalent malignancy with high mortality, involves lncRNA regulation, ferroptosis pathway abnormalities, and gut microbiota dysbiosis. Ferroptosis-related gene models may aid prognostic evaluation, while microbiota metabolites modulate ferroptosis in contexts like ulcerative colitis.

Methods: Using the GEO dataset (GSE97300), we screened differentially expressed lncRNAs (e.

Post-marketing safety analysis of blonanserin: a retrospective pharmacovigilance study using the Japanese adverse drug event report database (2008-2024).

Blonanserin, an atypical antipsychotic approved in Japan for the treatment of schizophrenia, exhibits a unique receptor affinity profile and a reportedly favorable safety profile; however, comprehensive large-scale real-world data on its adverse drug events (ADEs) remain limited. We conducted a retrospective pharmacovigilance study using the Japanese Adverse Drug Event Report (JADER) database from 2008 to 2024, focusing on ADEs where blonanserin was identified as the primary suspect drug. Safety signals were detected via disproportionality analyses employing both frequentist and Bayesian approaches and prioritized according to clinical relevance.

Mechanistic insights into the renoprotective effects of artesunate in cisplatin-induced acute kidney injury.

Acute kidney injury (AKI) is a severe clinical syndrome marked by rapid renal function decline. Cisplatin (Cis) is a commonly used chemotherapy agent; however, its clinical application has been restricted due to its nephrotoxicity. Artesunate (ART), derived from Artemisia annua, has potent anti-inflammatory and antioxidant properties.

Effects of Bt Rice Straw Extract on Seed Germination and Plant Growth of Pakchoi: Novel Variables of Cropping System.

The incorporation of (Bt) rice straw into fields may influence the growth of subsequent crops, but its ecological risks for winter vegetables remain largely unreported. Investigating the effects of Bt rice straw extracts on the seed germination and plant growth of pakchoi ( L. ssp.

Investigation into neuroleptic malignant syndrome triggered by atypical antipsychotics: Insights from FDA adverse event reporting system database.

Objective: Neuroleptic malignant syndrome (NMS) is a rare but potentially life-threatening reaction associated with dopamine antagonists, particularly typical antipsychotics. Despite the relatively low incidence of NMS linked to atypical antipsychotics (AAPs), there is a notable lack of extensive real-world studies investigating this condition.

Methods: This study utilizes data from the FDA Adverse Event Reporting System (FAERS) from 2004 to 2024, employing disproportionality analysis to explore the association between fourteen commonly prescribed AAPs and NMS.

Sophora compounds against non-small cell lung cancer: Research status and mechanisms.

Background: Non-small cell lung cancer (NSCLC) is the most prevalent form of lung cancer, characterized by dysregulated signaling pathways. Many Sophora compounds exhibit potential anti-NSCLC properties. However, the research status, particularly regarding the underlying mechanisms, remains fragmented.

Thermal-activated Polydopamine bilayer film exhibits dual-mode synergistic antibacterial properties for enhanced salmon preservation.

Foodborne pathogens threaten food safety and human health, prompting the need for innovative preservation solutions. Polydopamine endowed with photothermal properties represents a novel material for the inhibition of foodborne pathogens. This study develops a bilayer antibacterial film using polydopamine-berberine nanoparticles (PDA-BBR), leveraging dual-mode synergistic antibacterial effects through chemical and photothermal mechanisms.

Emodin ameliorates dopaminergic neuron loss in the MPP induced parkinson's disease model: significant inhibition of ferroptosis by activating UQCRC1 protein.

Emodin, a naturally occurring compound derived from anthraquinone, demonstrates notable efficacy in combating oxidative stress, protecting neural tissues, and inhibiting malignant cell proliferation. This bioactive phytochemical has garnered significant scientific attention due to its multifaceted therapeutic potential, particularly in the context of oncological interventions and neurodegenerative disorder management. The neuroprotective capacity of emodin in preventing dopaminergic neuronal demise through ferroptosis modulation requires further exploration.

Bacteriostatic Activity and Resistance Mechanism of Extract Against in Pepper.

The destructive bacterial wilt disease caused by leads to substantial losses in pepper production worldwide. Plant-derived pesticides exhibit advantages of high efficiency and broad spectrum when compared to traditional chemical pesticides. and 'Tai Jiao' No.

Elucidating the mechanism of the first Chinese herbal formula Shuangxia Decoction to alleviate insomnia using multi-omics technologies.

Background: Shuangxia Decoction (SXD), evolved from " Banxia Shumi Decoction", is composed of Pinellia ternata (Thunb.) Makino and Prunella vulgarisl. SXD has been used to treat insomnia and is considered the first traditional Chinese herbal formula developed specifically for the treatment of insomnia.

Exploration of the mechanism of tetramethoxyflavone in treating osteoarthritis based on network pharmacology and molecular docking.

Objectives: This study aimed to explore the potential mechanisms of TMF (5,7,3',4'-tetramethoxyflavone) in treating osteoarthritis (OA) using network pharmacology and molecular docking.

Materials And Methods: Databases including SwissTargetPrediction, BATMAN-TCM, PharmMapper, TargetNet, SuperPred, and SEA were utilized to screen the targets of TMF. "OA" was used as the disease keyword to predict OA-related genes through GeneCards, Therapeutic Target Database, PharmGKB, Online Mendelian Inheritance in Man, and Comparative Toxicogenomics Database.

Adverse drug events associated with metreleptin administration: a real-world pharmacovigilance study from 2014 to 2024 using the FAERS database.

Background: This study analyzed adverse drug event (ADE) signals associated with metreleptin using the FDA Adverse Event Reporting System (FAERS) to provide insights for safe clinical use.

Research Design And Methods: Data from 1 January 2014, to 31 March 2024, were extracted. Signal intensity for adverse events was assessed using reporting odds ratio (ROR), Medicines and Healthcare Products Regulatory Agency (MHRA), Bayesian Confidence Propagation Neural Network (BCPNN), and Multi-item Gamma Poisson Shrinker (MGPS) methods.

To Reveal the Potential Mechanism of Quercetin against NSCLC Based on Network Pharmacology and Experimental Validation.

Purpose: This study aimed to initially clarify the potential mechanism of quercetin in the treatment of non-small cell lung cancer (NSCLC) based on network pharmacology, molecular docking and in vitro experiments.

Method: TCMSP, SwissTargetPrediction, TCMIP, STITCH, and ETCM databases were applied to obtain the targets of quercetin. NSCLC-related targets were retrieved from GeneCards, OMIM, PharmGKB, TTD, and NCBI databases.

Integration of high-resolution mass spectrometry technology with molecular network analysis and systems biology techniques to elucidate the active ingredients and mechanisms of Shiduqing capsules.

Rationale: Shiduqing Capsules, a well-known Chinese patent medicine, are widely used clinically for the treatment of pruritus. However, to date, there is a lack of research on its pharmacological substances and mechanisms of action.

Methods: In the current study, the chemical components of Shiduqing Capsules were identified using UHPLC-QE-Orbitrap-MS technology.

Identification of active ingredients in Naomaitai capsules using high-resolution mass spectrometry unite molecular network analysis and prediction of their action mechanisms.

Rationale: Although Naomaitai capsule (NMC) is widely used in clinical practice and has a good curative effect for cerebral infarction, its material basis and mechanism of action remain unclear.

Methods: In this study, ultra-high-performance liquid chromatography (UHPLC) coupled with quadrupole Orbitrap MS technology was used to analyse the in vivo and in vitro components of NMC, and the Global Natural Products Social Molecular Networking website was used to further analyse the components of NMC. Next, systems biology approaches were employed to investigate the mechanism of action of NMC.

Development and Application of a Physiologically Based Pharmacokinetic Model for Elagolix in the Adult and Adolescent Population.

Introduction: Endometriosis, a common and distressing gynecological condition, affects fertility and causes pain, is often managed with medications such as Elagolix. The present study aimed to construct a physiologically based pharmacokinetic (PBPK) model for elagolix to predict its pharmacokinetics in different populations, including those with special conditions, to enhance treatment strategies for endometriosis.

Methods: The PBPK model was optimized using observational data based on the oral administration of elagolix in a healthy Chinese population under fasting conditions.

Integrating metabolomics and bioinformatics to reveal the mechanism of Epimedium-induced liver injury.

Epimedium is a traditional Chinese medicine with a wide range of clinical applications; however, there have been numerous reports of adverse reactions in recent years. The most common side effect of Epimedium is liver injury. In this study, the liquid chromatography-mass spectrometry (LC-MS) method has been established to study the components of Epimedium and to identify the components absorbed into the blood of rats.

Ultrahigh-performance liquid chromatography Q-Exactive-Orbitrap mass spectrometry and molecular network analysis alongside network pharmacology to elucidate the active constituents and mechanism of action of Huahong tablet in treating pelvic inflammatory disease.

Rationale: Huahong tablet, a commonly used clinical Chinese patent medicine, shows good efficacy in treating pelvic inflammation and other gynaecological infectious diseases. However, the specific composition of Huahong tablets, which are complex herbal formulations, remains unclear. Therefore, this study aims to identify the active compounds and targets of Huahong tablets and investigate their mechanism of action in pelvic inflammatory diseases.