PRMT5 encourages cell migration and metastasis of tongue squamous cell carcinoma through methylating ΔNp63α.

Tongue squamous cell carcinoma (TSCC) is a common oral malignancy prone to metastasis, whose underlying mechanism remains obscure. Here, we report the oncogenic roles of protein arginine methyltransferase 5 (PRMT5) in TSCC via inhibiting transcription factor ΔNp63α. We found that PRMT5 physically interacts with ΔNp63α, resulting in impairment of ΔNp63α-mediated transcriptional regulation.

Cyclin-dependent kinase 1 (CDK1) in cancers: from upstream regulation to downstream substrates and therapeutic inhibitors.

Cyclin-dependent kinase 1 (CDK1) is a pivotal regulator of cell cycle progression. Mounting documents indicate the complicated roles of this kinase in various malignancies. Here, we roundly overview the regulation of CDK1 in malignancies, and find that CDK1 is widely upregulated in diverse cancers at either expression or enzymatic activity levels.

Alternative splicing mediates immune-metabolic reprogramming associated with disease resistance in Apostichopus japonicus during Vibrio splendidus infection.

Alternative splicing (AS) is an evolutionarily conserved post-transcriptional mechanism that substantially expands transcriptomic and proteomic diversity in eukaryotes. However, its global regulatory landscape under pathogenic stress remains largely unexplored in non-model invertebrates. In this study, we conducted an in-depth analysis of alternative splicing responses in Apostichopus japonicus under Vibrio splendidus infection by integrating long-read transcriptomic sequencing with the SUPPA2 framework.

Protective Effects of Serum Containing Tibetan Medicine Jiangtangle on Mitochondrial Function in RIN-m5F Pancreatic β-Cells.

β-cell failure caused by mitochondrial dysfunction is the core feature of type 2 diabetes mellitus (T2DM). Tibetan medicine Jiangtangle, composed of Berberis kansuensis, Curcumaelongae Rhizoma, Malvae Fructus, Tribuli Fructus, Thlaspi arvense L., and Phyllanthus emblica L.

The value of triglyceride-glucose index-related indices in evaluating migraine: perspectives from multi-centre cross-sectional studies and machine learning models.

Background: This study employed representative data from the U.S. and China to delve into the correlation among migraine prevalence, the triglyceride‒glucose index, a marker of insulin resistance, and the composite indicator of obesity.

RNA-Seq Analysis Revealed the Virulence Regulatory Network Mediated by the Ferric Uptake Regulator (Fur) in Pathogenesis Induced by .

The uptake and utilization of iron by bacteria must be strictly controlled. The ferric uptake regulator (Fur) is a global transcription factor widely present in bacteria that can perceive cellular iron levels and adjust the expressions of various genes accordingly. Our earlier research demonstrated that the knockdown of the gene in significantly reduced its lethality to .

N6-methyladenosine-dependent upregulation of lnc-28509 increases apoptosis and suppresses bacterial infection in sea cucumber.

In recent years, lncRNAs (long non-coding RNAs) as well as m6A (N6-methyladenosine) modification have been found to play important roles during pathogens infection induced innate immune response. However, the relationship between the lncRNAs and m6A modification remains unclear. Here, we show that the lncRNA (lnc-28509) from sea cucumber Apostichopus japonicus was significantly induced in response to Vibrio splendidus infection.

The E3 ligase TRIM21 promotes progression of pancreatic ductal adenocarcinoma by down-regulating TAp63α and derepressing .

Pancreatic ductal adenocarcinoma (PDAC) is an aggressive tumor and frequently has mutations in the transcription factor p53. TAp63α is a member of the p53 protein family that is generally tumor suppressive in various other p53-mutant or p53-deficient cancers. Here, we found that TAp63α inhibited cell proliferation, epithelial-mesenchymal transition (EMT), and migration in several p53-mutant PDAC cell lines.

Vibrio splendidus type VI secretion system hemolysin-coregulated protein inhibits the inflammatory response of Apostichopus japonicus by interacting with major yolk protein 1.

Consistent with inflammation being an important host defense mechanism, bacterial pathogens have evolved strategies to evade or paralyze the inflammation system. Research indicates that the type VI secretion system (T6SS) is closely associated with the pathogenicity of bacteria. However, it remains unclear whether T6SS protein regulates coelomocyte inflammation during Vibrio splendidus infection and what the underlying molecular mechanisms are.

Identification and characterization of disease resistance-related SNPs using transcriptome analyses in sea cucumber Apostichopus japonicus.

Apostichopus japonicus is a commercially significant aquaculture species. However, in recent years, frequent outbreaks of skin ulcer syndrome (SUS) caused by Vibrio splendidus have resulted in substantial economic losses for the A. japonicus industry.

Comparative study of wear properties of CAD/CAM PEEK materials, resin ceramics and dental enamel under simulated chewing conditions.

Objectives: To compare the impact-sliding wear properties of CAD/CAM polyetheretherketone (PEEK) materials and resin ceramics with dental enamel, and to investigate the corresponding wear mechanisms.

Methods: Six CAD/CAM polymer materials were assessed in comparison with human tooth enamel. Hardness, modulus and roughness values of each group were measured, prior to wear testing in a chewing simulator (ball-on-disc design, 49 N).

A novel double Ig interleukin-1 receptor-related molecule from Apostichopus japonicus alleviates Vibrio splendidus-induced inflammation.

In vertebrates, the single immunoglobulin (Ig) interleukin-1 receptor-related molecule, SIGIRR, plays a role in controlling inflammatory responses. Its invertebrate homologous double Ig interleukin-1 receptor-related molecule, DIGIRR, is little known. We report here the cloning of a novel DIGIRR homologue in Apostichopus japonicus, named AjDIGIRR, using rapid amplification of cDNA ends (RACE).

Guanylate-binding proteins protect the sea cucumbers Apostichopus japonicus against infection of Vibrio splendidus.

Guanylate-binding proteins (GBPs) are interferon-induced innate immune effector molecules belonging to the GTPase superfamily and are crucial components of cell-autonomous immunity. To date, most research on GBPs has focused on mammals, with limited studies in marine invertebrates. In this study, we identified three GBP genes from the sea cucumber Apostichopus japonicus, designated as AjGBP1, AjGBP2, and AjGBP3.

Molecular characterization and immunological functions of the antimicrobial peptide theromacin from razor clam Sinonovacula constricta.

Antimicrobial peptides are natural immune effector molecules that protect the host from pathogenic microorganisms and play crucial roles in the innate immune system. Herein, the functions of theromacin from the razor clam Sinonovacula constricta (ScTM) were identified. Multiple sequence alignment revealed that the structure of ScTM was similar to that of a theromacin homolog with a signal peptide and conserved Macin domain.

Potential roles of the melatonin system in the promotion of ovarian estradiol secretion in the sea cucumber, Apostichopus japonicus.

Melatonin (MT), synthesized from tryptophan, modulates reproduction through its interaction with melatonin receptors (MTNRs). While its functions in vertebrates are well established, the MT system remains poorly understood in echinoderms. To elucidate MT synthesis regulation, we examined AjAsmt, a pivotal enzyme in MT biosynthesis, to determine its tissue-specific distribution in Apostichopus japonicus.

Lytic coelomocyte death is tuned by cleavage but not phosphorylation of MLKL in echinoderms.

Lytic cell death including necroptosis and pyroptosis is induced by mixed lineage kinase domain-like protein (MLKL) phosphorylation and inflammatory caspase specific cleavage Gasdermins in higher mammals, respectively. In this study, we identified a novel MLKL homolog containing a tetrapeptide recognition motif (14-LVAD-17) of inflammatory caspase from Apostichopus japonicus,which was absent of Gasdermins member by genome screening. Functional analysis revealed that AjMLKL was involved in the regulation of Vibrio splendidus AJ01 infection induced lytic coelomocyte death in a cleavage-dependent manner, but not through RIPK3-dependent phosphorylation as mammals.

Eeukaryotic-like Sppsk1 from Vibrio splendidus AJ01 mediates phagosome escape via inhibiting phagosome acidification and maturation.

The intracellular pathogen has evolved sophisticated mechanisms to evade host immune defenses by secreting different virulence factors. In our previous study, the eukaryotic factor STPKLRR was identified from the intracellular pathogen Vibrio splendidus AJ01 and shown to facilitate promote AJ01 internalization by mediating actin-dependent coelomocytes phagocytosis. However, the molecular mechanisms underlying AJ01'escaped from the phagosome remained largely unclear.

Evolutionary and functional insights into Fibrinogen-related protein (FREP) dynamics in sea cucumbers.

The Fibrinogen-related protein (FREP) gene family is a cornerstone of immune defense in metazoans, yet its evolution and functional specialization in invertebrates, particularly sea cucumbers, remain underexplored. By analyzing genomic data from 1004 species, this study reveals a significant and lineage-specific expansion of FREP genes in sea cucumbers. These genes are categorized into single-domain FREPs (sFREPs) and multi-domain FREPs (mFREPs), with the latter containing unique TIL domains associated with immune defense.

Age-specific and sex-specific associations of visceral adipose tissue with metabolic health status and cardiovascular disease risk.

Background: Visceral adipose tissue (VAT) is known to play a role in the development of metabolic and cardiovascular disease (CVD). However, the age- and sex-specific associations between VAT and these diseases remain unclear.

Methods: In this cross-sectional study, 1,150 participants (39.

Autophagy mediated by ROS-AKT-FoxO pathway is required for intestinal regeneration in echinoderms.

Autophagy is essential for maintaining material balance and energy circulation and plays a critical role as a regulatory mechanism in tissue regeneration. However, current studies primarily describe this phenotype, with limited exploration of its molecular mechanisms. In this study, we provided the first evidence that autophagy is required for intestinal regeneration in Apostichopus japonicus and identified a previously unrecognized regulatory mechanism involved in this process.

ΔNp63α promotes radioresistance in esophageal squamous cell carcinoma through the PLEC-KEAP1-NRF2 feedback loop.

Esophageal squamous cell carcinoma (ESCC) is one of the most aggressive cancers and is highly prevalent in China, exhibiting resistance to current treatments. ΔNP63α, the main isoform of p63, is frequently amplified in ESCC and contributes to therapeutic resistance, although the molecular mechanisms remain unknown. Here, we report that ΔNP63α is highly expressed in ESCC and is associated with radioresistance by reducing ROS level.

Pathogen-induced apoptosis in echinoderms: A review.

Echinoderms possess unique biological traits that make them valuable models in immunology, regeneration, and developmental biology studies. As a class rich in active substances with significant nutritional and medicinal value, echinoderms face threats from marine pathogens, including bacteria, viruses, fungi, protozoa, and parasites, which have caused substantial economic losses in echinoderm aquaculture. Echinoderms counteract pathogen invasion through innate immunity and programmed cell death, in particular, with apoptosis being essential for eliminating infected or damaged cells and maintaining homeostasis in many echinoderm cell types.

Growth stage-related capsular polysaccharide translocon Wza in Vibrio splendidus modifies phage vB_VspM_VS2 susceptibility.

Bacteria at different growth stages usually coordinate capsular polysaccharide (CPS) formation and may affect their susceptibility to phage. In this study, we evaluated the infection efficacy of phage vB_VspM_VS2 in V. splendidus AJ01 at different growth stages and explored the role of growth stage-related CPS translocon Wza in the susceptibility of V.