Recent Advances in Technologies for Preserving Fresh-Cut Fruits and Vegetables.

Rapid economic growth and changing consumer patterns have made fresh-cut fruits and vegetables household staples because of their high nutritional value, their role in reducing the risk of illnesses and other health problems, and convenience. However, fresh-cut produce is susceptible to the rapid deterioration of sensory quality, nutrient loss, foodborne pathogens contamination, and spoilage caused by microbial growth, which can lead to consumer health risks. Thus, there is an urgent need to improve preservation methods, to increase the shelf life of fresh-cut produce.

Genetic influence of the brain on epigenetic age acceleration: evidence of a large-scale genetic correlation study.

The relationship between the brain and aging remains unclear. Our objective is to explore the causal connections between brain structure,gene expression, and traits associated with aging. Mendelian randomization(MR) analysis was conducted to explore the associations between brain structures and aging-related traits including GrimAge acceleration(GrimAA), PhenoAge acceleration (PhenoAA), HannumAge acceleration(HannumAA), HorvathAge acceleration(HorvathAA), and leukocyte telomere length(LTL).

A human intestinal organoid derived from fetal human colon cells model for studying enteroviral pathogenesis.

Human enteroviruses, including enterovirus 71 (EV71), cause hand, foot, and mouth disease (HFMD) and may lead to severe neurological diseases in infants. Enteroviruses first infect the gastrointestinal tract and then spread to the main organs, such as the liver, lungs, heart, and brain. Human intestinal organoids (HIOs) provide a physiologically relevant model for studying enterovirus infections.

Comparative Metagenomics Reveals Microbial Diversity and Biogeochemical Drivers in Deep-Sea Sediments of the Marcus-Wake and Magellan Seamounts.

Seamounts are distributed globally across the oceans and are generally considered oases of biomass abundance as well as hotspots of species richness. Diverse microbial communities are essential for biogeochemical cycling, yet their functional partitioning among seamounts with geographic features remains poorly investigated. Through metagenomic sequencing and genome-resolved analysis, we revealed that Proteobacteria (33.

YY1-glycolytic axis promotes high glucose-induced cancer stemness in endometrial cancer: A multi-omics guided therapeutic strategy.

Abnormal glucose metabolism in patients with diabetes mellitus or hyperglycaemia is a key factor leading to poor prognosis of endometrial cancer (EC) and warrants further exploration of its underlying mechanisms. In this study, we employed an integrative multiomics approach, including proteomics, transcriptomics, nontargeted metabolomics and single-cell metabolomics, and revealed that high glucose promotes EC cell stemness, thereby promoting tumour progression and reducing the sensitivity of EC cells to cisplatin (platinum-Pt). Mechanistically, there is a significant association between enhanced stemness and increased glycolytic activity in EC cells, and the transcription factor YY1 was found to be a key regulator of PDK1, CD133, and CD44 under high-glucose conditions, with YY1 binding to their promoter regions.

A Multicenter Randomized Controlled Study on Pharmacokinetic-Guided Vancomycin Use in Children With Severe Infections.

This study is a multicenter, randomized controlled prospective trial aimed at evaluating the effects of two vancomycin pharmacokinetics/pharmacodynamics (PK/PD) parameters on clinical outcomes in children with different severe infections: trough concentration (C) and the area under the curve (AUC/MIC). From January 2023 to December 2024, 472 pediatric patients from seven hospitals in Southwest China were included in the present study. These patients were randomly assigned to the AUC/MIC group or the C group.

Fabric-based composite membrane for simultaneous emulsion separation and self-powered real-time monitoring.

Membrane separation technology is widely used in emulsion separation due to its high efficiency, energy saving, and environmental protection. However, Membrane contamination issues are not identified on time, which affects separation efficiency and the overall process. Real-time monitoring of the membrane separation process is essential for timely detection of membrane performance degradation and cost savings.

M6A-mediated uc003pes.1 stability regulates proliferation and migration of human coronary artery smooth muscle cells via scaffolding STAT1 and USP10.

Background: The uc003pes.1 has been identified as a potential diagnostic biomarker for coronary artery disease (CAD) and is associated with the risk of CAD. However, the precise function and intricate mechanism of uc003pes.

Long non-coding RNA LINC02499 regulates the expression of CYP3A5 in human liver cells.

Cytochrome P450 3A5 (CYP3A5) is a critical drug-metabolizing enzyme in human hepatocytes, playing a key role in the metabolism of various clinically relevant drugs, with considerable variability in gene expression across individuals. Long non-coding RNAs (lncRNAs) are likely to be important regulators within the CYP3A5 regulatory network in the liver. In this study, we employed a computational approach to estimate Sobol's sensitivity indices (SSI) under generalized linear models, applied to liver RNA expression microarray data (GTEx v8).

Clinical features of benign paroxysmal positional vertigo in the elderly.

Objectives: The typical age of benign paroxysmal positional vertigo (BPPV) is between 50 and 60 years. With the development of diagnostic techniques and the growth of the elderly, the number of elderly patients has been on the rise gradually. This study compared the clinical characteristics, treatments, and prognoses with patients.

The Tetracycline-Inducible/CRISPR-Cas9 System is an Efficient Tool for Studying Gene Function in Phytophthora sojae.

The present study presents a novel approach combining a tetracycline-inducible system (Tet-On) and CRISPR-Cas9 techniques to investigate the function of two essential genes in Phytophthora sojae. We constructed a donor vector in which the reverse tetracycline transactivator (rtTA) is driven by an oomycete promoter. Additionally, it contains a fused TetR binding site and the minimum oomycete promoter, as well as 1000-bp homologous arms of the promoter upstream and downstream sequences.

A study of the impact of DIP payment reform on coronary heart disease hospitalization costs and equity.

Background: To control the growth of healthcare costs, the Chinese government introduced a diagnosis-intervention package (DIP)-based health insurance payment reform. This study evaluated the impact of the DIP policy on hospitalization costs, Length of Hospital Stay, and Out-of-Pocket Ratio for patients with coronary heart disease (CHD).

Methods: Hospitalization claims data from 2020 to 2023 in City S, central China, were selected and analyzed using interrupted time series (ITS), covering 264 hospitals with January 2022 as the intervention point.

Physical and psychological health of medical students: A structural equation modeling approach.

Objective: This study aims to expand the application of behavioral immune system theory during public health emergencies and explore potential strategies to avoid "health poverty" in the post-pandemic era, focusing on Chinese medical students.

Methods: A comprehensive conceptual model was developed based on behavioral immune system theory. Data were collected through a cross-sectional survey conducted between September 2020 and June 2021, involving 1015 medical students in China.

Two novel polyvalent phages: a promising approach for cross-order pathogen control in aquaculture.

Bacteriophages represent a promising alternative to antibiotics for controlling bacterial pathogens. However, phage application is often hindered by its narrow host range in preventing diseases caused by multiple unknown pathogens. While broad-host-range phages capable of cross-genus or cross-order infections, offer significant advantages in addressing this challenge, they are rarely isolated.

Cellulase hyperproduction Trichoderma reesei mutant strain BB8 for the bioextraction and bioconversion of ginsenoside from Panax notoginseng.

Ginsenoside is an important bioactive substance from Panax notoginseng. However, the extraction efficiency of ginsenoside is very low. Here, we constructed a Trichoderma reesei mutant strain BB8 from T.

Early pulmonary rehabilitation recommended decision-making behavior experience among pediatric intensive care unit medical staff: a qualitative study.

Background: To understand the reasons for hindering and promoting the recommended decision-making behaviors for early pulmonary rehabilitation of PICU medical staff, and to provide a basis for developing corresponding management plans.

Methods: Based on the Capability, Opportunity, Motivation-Behavior (COM-B) model and Theoretical Domains Framework (TDF), interview outlines were developed. A descriptive qualitative research method was used, and a purposive sampling method was employed to select medical staff from the intensive care unit of a tertiary children's hospital in Nanjing from September to December 2023 for semi-structured interviews.

Enhanced thermotolerance via overexpression of a stromal ascorbate peroxidase in Nannochloropsis oceanica.

Overexpression of the heat-responsive ascorbate peroxidase gene NoAPX2430 improves thermotolerance and growth of Nannochloropsis oceanica, offering potential strategies for enhanced resilience in plant cells. Microalgae are promising for industrial lipid production but face challenges from high-temperature (HT) stress. This study focused on improving thermotolerance in Nannochloropsis oceanica by studying and modifying the heat-responsive ascorbate peroxidase gene, NoAPX2430.

Spatiotemporal dynamics and driving factors of human resources for health in traditional Chinese medicine in China.

The uneven distribution of human resources for health (HRH) in China, particularly within the realm of traditional Chinese medicine (TCM), has long posed a significant challenge. Although prior studies have examined regional disparities in overall HRH, limited research has specifically addressed the spatial and temporal dynamics of HRH in TCM and their underlying determinants. This study employs a multiscale geographically weighted regression (MGWR) model to explore the spatial and temporal dynamics of HRH in TCM across 31 Chinese provinces from 2008 to 2021.

Finite element analysis of vaginal tape at different positions for the treatment of arcus tendineus fascia pelvis damage-induced stress urinary incontinence.

Background: The purpose of this study was to use the finite element analysis method to simulate sling surgeries for arcus tendineus fascia pelvis damage-induced stress urinary incontinence: tension-free vaginal tape and tension-free vaginal tape - obturator, to evaluate their therapeutic effects at different positions of the urethra, and to judge the occurrence of complications through the sling-urethra interaction force which is difficult to obtain clinically.

Methods: Pelvic geometric model was constructed based on the MRI of a woman without pelvic floor diseases. The constraints between arcus tendineus fascia pelvis and the lateral wall of the vagina were removed to simulate arcus tendineus fascia pelvis rupture.

Impact of Diagnosis-Intervention Packet (DIP) reforms on inpatient services for low-income populations in central China: A multi-stage interrupted time-series analysis.

As global healthcare costs continue to rise, concerns about health equity have become increasingly prominent. In response, China introduced the Diagnosis-Intervention Packet (DIP) reform in 2021 to optimize healthcare resource allocation and control costs. While the reform has been widely discussed in terms of its overall cost-control effects, its heterogeneous impact on low-income populations, especially across different hospital tiers, remains unclear.

Impact of diagnosis-intervention packet payment on the consistency of hospitalization expenses across different medical insurance schemes in China.

Background: The Diagnosis-Intervention Packet Payment (DIP) system is regarded as a localized cost-control strategy in China. It aims to improve healthcare efficiency, curb the growth of medical expenses, and optimize the allocation of medical resources among diverse groups.

Objective: This study aims to assess the impact of DIP payment reforms on differential changes in patients' hospitalization expense and to explore the degree of concentration of hospitalization expense for patients with different insurance schemes undergoing treatment for typical diseases, with a view to providing policy recommendations for improving the medical insurance system.

A wearable self-aid microneedle chip based on actively transdermal delivery of epinephrine.

Epinephrine is important for first aid and usually applied via injection, which is painful and problematic in operation, thereby making it difficult to self-delivery. In this study, a method to actively transdermal delivery of epinephrine is proposed based on incorporating microneedles with iontophoresis, and then a wearable device is fabricated for rapid and controllable self-delivery of epinephrine. The device consists of a hydrogel microneedle array, a conductive drug delivery hydrogel, iontophoresis electrodes, and an encapsulated cartridge with a spring and self-locking micro-mechanism.

Wide-linearity flexible OFET biosensors for wearable biomarkers detection.

Flexible organic field-effect transistor (OFET) sensors, which leverage conjugated π-bonds in organic semiconductor layers to facilitate rapid charge transfer and enhance sensing sensitivity, offer significant advantages for detecting low concentrations of biomarkers in wearable biomedical electronics, such as glucose monitoring for diabetes. However, conventional OFET sensors suffer from a narrow linear range due to limitations in threshold voltage and saturation current. Therefore, a common problem in the field of the OFET-based biomarker sensing is that the narrow linear range of these sensors fails to meet detection requirements.

Perioperative nivolumab and chemotherapy in locally advanced squamous cell carcinoma of the oesophagus: a randomized multicentre phase 2 study with circulating tumor DNA dynamics monitoring.

Background: Although neoadjuvant chemotherapy and immunotherapy show promise in treating oesophageal squamous cell carcinoma (OSCC), long-term survival data are limited. This randomized, multicenter phase 2 study evaluated the efficacy of perioperative Nivolumab with chemotherapy, followed by surgery and adjuvant immunotherapy, in patients with locally advanced resectable OSCC, and explored the prognostic role of circulating tumor DNA (ctDNA) status.

Methods: In this trial, participants recruited from five centers were randomly assigned in a 2:1 ratio to receive either perioperative Nivolumab or a placebo in addition to chemotherapy (cisplatin and paclitaxel), followed by minimally invasive esophagectomy.

Improving lower-extremity artery depiction and diagnostic confidence using dual-energy technique and popliteal artery monitoring in dual-low dose CT angiography.

To assess the utility of dual-energy CT scanning (DECTs) with popliteal artery (PA) monitoring in dual low-dose (radiation and contrast) lower-extremity CT angiography (LE-CTA). 135 patients undergoing LE-CTA were prospectively included and divided into three groups of 45 each. Group-A: conventional scanning, 105 mL of contrast, abdominal aorta monitoring; Group-B: low-dose DECTs, 95 mL of contrast, AA monitoring; Group-C: low-dose DECTs, 85 mL of contrast, PA monitoring.