Cu(II)-catalyzed synthesis of 3-functionalized benzofurans sequential nucleophilic cyclization and C-C bond activation.

A domino Cu-catalyzed nucleophilic cyclization and C-C bond activation to access various 3-acylated benzofurans has been developed. The generated benzofuranylcopper intermediate was coupled with cyclopropenone, wherein the ring-opening of cyclopropenone with phenol was effectively suppressed. This strategy features excellent substrate scope, good functional group tolerance, and high atomic economy.

High-throughput discrimination of chlorogenic acid isomers in Lonicerae Japonicae Flos by U-shaped mobility analyzer-mass spectrometer.

Chlorogenic acids (CGAs), bioactive polyphenols with structural similarities and labile characteristics, pose analytical challenges for isomer differentiation. This study introduces a rapid U-shaped mobility analyzer-mass spectrometry (UMA-MS) method for high-resolution characterization of 13 CGAs, including five isomeric groups. The technique achieved complete separation within 2 s, with a total analysis time of 2 min per sample.

Diagnostic performance of Node-RADS on MRI for a standardized assessment of lymph node metastasis in ovarian cancer.

Objective: The Node-RADS score was proposed and provided a standardized, comprehensive assessment of lymph nodes (LNs), accounting for both size and configuration criteria. This study aimed to evaluate the diagnostic performance of the Node-RADS in LN metastasis of ovarian cancer (OC).

Methods: From December 2018 to April 2023, 81 OC patients who underwent MRI and debulking surgery were included.

ZmMYB155 is involved in starch synthesis and basal endosperm transfer layer development in maize.

ZmMYB155 acts as an important regulatory factor in starch synthesis and BETL development in maize. Starch synthesis is a critical process determining both the grain quality and quantity of a crop. The basal endosperm transfer layer (BETL) plays a crucial role in nutrient transport and defense against pathogens.

Identification of energy metabolism and m6A-related gene clusters in Kashin-Beck disease based on bioinformatics analysis.

Kashin-Beck disease (KBD) is a chronic and debilitating osteoarthropathy predominantly affecting populations in certain endemic regions. Despite extensive research, the underlying mechanisms of KBD remain poorly understood. Here we analyzed gene expression profiles from KBD patients and healthy controls, identifying 16 differentially expressed genes.

Glaesserella parasuis infection triggers endoplasmic reticulum stress-mediated pyroptosis via PERK/eIF2α/ATF4 axis and metabolic reprogramming in porcine alveolar macrophages.

Glaesserella parasuis, the causative agent of Glässer's disease in swine, triggers severe systemic inflammation; however, the molecular mechanisms underpinning its pathogenesis remain incompletely understood. This study investigated the cellular and metabolic responses of porcine alveolar macrophage 3D4/21 cells to G. parasuis infection.

deletion inhibits -mediated inflammatory responses in porcine alveolar macrophages.

(GPS) infection causes severe inflammatory disorder, resulting in lung injury. is an NAD-dependent deacetylase known to regulate inflammatory responses, but its role in GPS infection remains unclear. Here we found that GPS infection increased expression and induced inflammatory responses.

D-RADA16 self-assembling peptide microneedle patches alleviate osteoarthritis inflammation by inhibiting mtDNA leakage-mediated cGAS-STING pathway.

Mitochondrial DNA (mtDNA) leakage and its activated cGAS-STING signaling pathway play a critical role in chronic inflammation of osteoarthritis (OA). However, how to effectively suppress mtDNA leakage and maintain joint homeostasis remains a huge challenge. This study proposes a synergistic strategy that combines the bioactive, extracellular matrix (ECM)-mimicking properties of D-RADA16 self-assembling peptides with the minimally invasive, localized delivery capabilities of a dissolvable microneedle patch (MNP).

Impact of cardiac phase selection on computational fluid dynamics analysis.

Background: The selection of cardiac phase in coronary computed tomography angiography (CCTA) may affect computational fluid dynamics (CFD)-derived hemodynamic metrics; however, this influence is not well-quantified, particularly in anomalous coronary anatomies. This study aims to evaluate how cardiac phase selection impacts CFD outcomes in normal and anomalous coronary arteries.

Methods: Multiphase CCTA datasets were analyzed from 40 patients: 30 with systolic and diastolic reconstructions (10 inter-arterial anomalous right coronary artery [ARCA], 10 myocardial bridging [MB], 10 normal) and 10 additional normals with mid-diastolic and diastolic reconstructions.

Porcine SCD1 Regulates Lipid Droplet Number via CLSTN3B in PK15 Cells.

Fat deposition plays a key role in determining porcine meat quality traits, with lipid droplets serving as critical organelles for lipid storage in adipose tissue. Inhibiting lipid droplet biogenesis disrupts the lipid storage capacity of adipocytes. The Stearoyl-CoA Desaturase () family is crucial in regulating polyunsaturated fatty acid/monounsaturated fatty acid (PUFA/MUFA) composition, while its role in lipid droplet formation remains unclear.

The Crucial Role of Oxygen Evolution Reaction in Electrocatalytic Oxidative C─C Bond Cleavage in Lignin Biomass Valorization.

The electrocatalytic oxidative cleavage of the C─C bond is highly effective for converting lignin biomass into high-value-added aromatic chemicals. However, the oxygen evolution reaction (OER) at applied oxidative potential usually competes in the aqueous environment. In this study, we found that OER does not entirely hinder the cleavage process but rather acts as a synergistic catalytic step.

mpMRI-based habitat analysis for predicting prognoses in patients with high-grade serous ovarian cancer: a multicenter study.

Purpose: To evaluate the value of multiparametric MRI (mpMRI)-based habitat analysis for predicting prognoses in patients with high-grade serous ovarian cancer (HGSOC), and to develop combined models by integrating habitat analysis with clinical predictors.

Methods: This retrospective study included 503 HGSOC patients from four centers. A K-means algorithm was used to identify voxel clusters and generate habitats on mpMRI.

A multi-modal model integrating MRI habitat and clinicopathology to predict platinum sensitivity in patients with high-grade serous ovarian cancer: a diagnostic study.

Background: Platinum resistance of high-grade serous ovarian cancer (HGSOC) cannot currently be recognized by specific molecular biomarkers. We aimed to compare the predictive capacity of various models integrating MRI habitat, whole slide images (WSIs), and clinical parameters to predict platinum sensitivity in HGSOC patients.

Methods: A retrospective study involving 998 eligible patients from four hospitals was conducted.

Chlorine evolution characteristics during lab-scale combustion of MSW pyrolysis char obtained from 200 t/d pyrolysis demonstration plant.

Pyrolysis is an effective method for treating municipal solid waste (MSW). Chlorine in MSW causes the production of dioxins, chlorine gas (Cl), hydrogen chloride (HCl) and some other chlorine-containing compounds, and these chlorine-containing compounds are also very harmful to the environment and human health. This study investigates chlorine evolution during the combustion of chlorine-rich MSW pyrolysis char obtained from a 200 t/d MSW pyrolysis demonstration (DEMO) project under industrial conditions.

MRI assessment of sacral injury location and analysis of influencing factors after high-intensity focused ultrasound ablation for patients with uterine fibroids.

Purpose: Exploration of the location of sacral injuries following ultrasound-guided high-intensity focused ultrasound (USgHIFU) ablation for uterine fibroids and analysis of its influencing factors.

Methods: A retrospective analysis was conducted on 663 patients with uterine fibroids treated by USgHIFU ablation. Patients with vertebral injuries were identified based on postoperative MRI images, with specific locations of the injuries documented.

Foundation model-driven multimodal prognostic prediction in patients undergoing primary surgery for high-grade serous ovarian cancer.

High-grade serous ovarian cancer (HGSOC) presents challenges in prognostic prediction. This study aimed to develop a universal foundation model-driven multimodal model (FoMu model) to assess the prognosis of HGSOC patients. We conducted a retrospective cohort study involving 712 eligible patients across four centers, collecting clinical, MRI, and hematoxylin and eosin (H&E)-stained whole slide images (WSIs) data.

Transcriptomic and Lipidomic Characteristics of Subcutaneous Fat Deposition in Small-Sized Meat Ducks.

Subcutaneous fat deposition is associated with ducks' meat quality and the methods used to cook them. However, the reasons underlying the differences in the lipid deposition of small-sized Wuqin10 meat ducks remain unclear. In the present study, to elucidate the metabolic mechanisms of lipid deposition, we comprehensively analyzed the transcriptomics and lipidomics of subcutaneous fat in Wuqin10 meat ducks with different subcutaneous thicknesses with six replicates.

PDE4D inhibition ameliorates cardiac hypertrophy and heart failure by activating mitophagy.

Cyclic adenosine monophosphate (cAMP) plays a major role in normal and pathologic signaling in the heart. Phosphodiesterase 4 (PDE4) is a major PDE degrading cAMP in the heart. There are inconsistencies concerning the roles of the PDE4 isoforms 4B and 4D in regulation of cardiac function.

Recycling excavated soil waste into low-carbon alkali-activated materials.

Rapid urbanization generates billions of tons of excavated soil waste annually from underground developments, presenting significant sustainability challenges. The objective of this study was to develop a sustainable recycling method for soil waste. Soil waste was calcined to transform its kaolin content into metakaolin.

Integrative Transcriptomics and Proteomics Analysis Reveals 's Role in Lipid Metabolism.

Abnormalities in lipid metabolism and endoplasmic reticulum (ER) stress are strongly associated with the development of a multitude of pathological conditions, including nonalcoholic fatty liver disease (NAFLD), diabetes mellitus, and obesity. Previous studies have indicated a potential connection between thyroid hormone responsive ( and lipid metabolism and that ER stress may participate in the synthesis of key regulators of adipogenesis. However, the specific mechanisms remain to be investigated.

Innovative dual-benefit recycling and sustainable management of municipal solid waste incineration fly ash via ultra-high performance concrete.

Millions of tons of municipal solid waste incineration fly ash are generated worldwide each year. Currently, landfilling is the primary disposal method, consuming vast land resources and posing significant risks of soil and groundwater contamination due to the high concentration of hazardous heavy metals. Thus, this study investigated the potential of recycling hazardous municipal solid waste incineration fly ash into non-hazardous ultra-high performance concrete for sustainable development.

Multi-point fiber optic laser-ultrasonic transducer based on long-period fiber grating.

For ultrasonic non-destructive testing, the development of miniaturized, embeddable, and distributed ultrasonic transducers has always been a hot research topic. In light of this, a novel fiber optic laser-ultrasonic transducer, employing the long-period fiber grating (LPFG), was introduced in this study to facilitate the implementation of embedded and distributed applications. LPFG couples the light transmitted in the optical fiber into the photoacoustic conversion material.

Deoxynivalenol-Induced Spleen Toxicity in Mice: Inflammation, Endoplasmic Reticulum Stress, Macrophage Polarization, and the Dysregulation of LncRNA Expression.

The spleen is a primary target of deoxynivalenol (DON) toxicity, but its underlying molecular mechanisms remain unclear. This study investigates the effects of DON on inflammation, splenic macrophage polarization, endoplasmic reticulum (ER) stress, and transcriptome changes (mRNA and lncRNAs) in mouse spleen. We found that DON exposure at doses of 2.

Reshaped Local and Systemic Immune Responses Triggered by a Biomimetic Multifunctional Nanoplatform Coordinating Multi-Pathways for Cancer Therapy.

Immunotherapy has fundamentally transformed the clinical cancer treatment landscape; however, achieving intricate and multifaceted modulation of the immune systems remains challenging. Here, a multipathway coordination of immunogenic cell death (ICD), autophagy, and indoleamine 2,3-dioxygenase-1 (IDO1) was achieved by a biomimetic nano-immunomodulator assembled from a chemotherapeutic agent (doxorubicin, DOX), small interfering RNA (siRNA) molecules targeting IDO1 (siIDO1), and the zeolitic imidazolate framework-8 (ZIF-8). After being camouflaged with a macrophage membrane, the biomimetic nanosystem, named mRDZ, enriched in tumors, which allowed synergistic actions of its components within tumor cells.