Uncovering the resistome and mobilome across different types of ready-to-eat fermented foods.

Antimicrobial resistance in food poses a significant threat to public health, and the persistence of antibiotic resistance genes (ARGs) in ready-to-eat fermented foods (RTE-FFs) is a growing concern. However, information on the diversity, origins, and transferability of ARGs in RTE-FFs is limited. This study investigated the distribution of ARGs and mobile genetic elements (MGEs) in four types of RTE-FFs: soybean, dairy, meat, and vegetable products.

Network pharmacology and experimental validation to elucidate the pharmacological mechanisms of OATF against kidney stones.

Introduction: (Blume) Miq. (OA) is widely used in folk medicine to treat kidney stones (KS). Its total flavonoids (OATF) are the primary active constituents responsible for its therapeutic effects.

Effects of Licorice Stem and Leaf Forage on Growth and Physiology of Hotan Sheep.

This study explored the impact of licorice stem and leaf forage in the diet of meat sheep on their growth performance, physiological parameters, immunity, and gut microbiota. Conducted in the Hotan region of Xinjiang, the experiment involved 40 healthy local Hotan sheep (5-6 months old) that were randomly assigned to four groups, with both corn stalks and corn grain partially substituted at varying levels (30%, 40%, and 50%) by licorice stems and leaves for 80 days. Daily feed intake and refusal were recorded to assess feeding behavior and efficiency, while body weight was measured every 20 days to monitor growth performance.

Development of an Asymmetric Alginate Hydrogel Loaded with S-Nitrosoglutathione and Its Application in Chronic Wound Healing.

Nitric oxide (NO) is an endogenous signaling molecule that plays a critical role in wound healing. However, the gaseous nature, short half-life, and low stability of NO present challenges for its clinical application. To address these issues, this study introduces an innovative S-nitrosoglutathione (GSNO)-loaded asymmetric alginate (SA) hydrogel (GSNO-SA) as a novel solution for treating infected chronic wounds.

Silver nanoparticles derived from Streptomyces sp. YJD18 with multifunctional biomedical applications.

A sustainable and energy-efficient approach was developed for the biosynthesis of silver nanoparticles (AgNPs) using a cell-free supernatant derived from Streptomyces sp. strain YJD18, an actinomycete isolated from saline soil. Optimized synthesis conditions, including pH 10 and a biomass concentration of 5 g/100 mL, facilitated the rapid formation of AgNPs.

A Novel Benzoxazole-Functionalized Conjugated Mesoporous Polymer for Highly Sensitive and Selective Detection of Fe and Fe.

Conjugated micro/mesoporous polymers (CMPs) have emerged as versatile platforms for designing advanced functional materials. In this study, a novel benzoxazole-functionalized CMP, TFPA-DBD, was rationally synthesized, featuring a spherical morphology, well-defined mesoporous structure and robust thermal/chemical stability. When dispersed in DMF, TFPA-DBD exhibits a selective "turn-off" fluorescence response toward Fe and Fe ions, achieving low detection limits (LOD) of 0.

BRD4 regulates mA of ESPL1 mRNA interaction with ALKBH5 to modulate breast cancer progression.

The interaction between mA-methylated RNA and chromatin modification remains largely unknown. We found that targeted inhibition of bromodomain-containing protein 4 (BRD4) by siRNA or its inhibitor (JQ1) significantly decreases mRNA mA levels and suppresses the malignancy of breast cancer (BC) cells increased expression of demethylase AlkB homolog 5 (ALKBH5). Mechanistically, inhibition of BRD4 increases the mRNA stability of ALKBH5 enhanced binding between its 3' untranslated regions (3'UTRs) with RNA-binding protein RALY.

fermentation characteristics of polysaccharide from and its effects on type 2 diabetes mellitus gut microbiota.

Background: Increasing evidence has shown a close relation between the pathogenesis of type 2 diabetes mellitus (T2DM), which is a global health problem with multifactorial etiopathogenesis, and gut microbiota.

Methods: During fermentation of (known as Xuanshen) polysaccharide (SNP) by T2DM gut microbiota, effects of SNP on the gas content, production of short-chain fatty acids (SCFAs), metabolite profile and microbiota composition were studied.

Results: Analysis of chemical compositions indicates that the total sugar content of SNP was found to be as high as 87.

MicroRNA-214-3p Delivered by Bone Marrow Mesenchymal Stem Cells-Secreted Exosomes Affects Oxidative Stress in Alzheimer's Disease Rats by Targeting CD151.

Objective: This study probed the effect of targeted regulation of CD151 by microRNA-214-3p (miR-214-3p) delivered by bone marrow mesenchymal stem cells-secreted exosomes (BMSCs-exo) on oxidative stress and apoptosis of neurons in Alzheimer's disease (AD).

Methods: Rat BMSCs were isolated, from which MSCs-exo were extracted and identified. The AD rat model was established and injected with MSC-exo suspension.

Preparation of Quercetin/Copper Nanoparticles and Their Preservation Performance on Grapes.

Deterioration in fruits represent a significant challenge to food safety, which has prompted our investigation into sustainable fruit preservation technologies. This paper presents the synthesis of quercetin/copper nanoparticles (QC NPs) and their application in the preservation of grapes. The QC NPs, prepared through quercetin/copper complexation, exhibited stability with a particle size of 79.

Magnolia essential oil: a preliminary exploration of chemical composition and its antimicrobial and antioxidant potential.

In this study, the chemical composition of Magnolia essential oil (MEO) was analyzed using gas chromatography-mass spectrometry (GC-MS). The results indicated that terpenoids were the primary constituents, with the main components being 1,8-cineole (44.87%), (+)-citronellal (6.

Microglia specific Csf1r haploinsufficiency induces depressive-like behaviors by promoting NLRP6/caspase-1 signaling in mice.

Depression is an early clinical manifestation of adult-onset leukoencephalopathy with axonal spheroids and pigmented glia (ALSP), although the underlying molecular mechanisms remain poorly elucidated. The objective of this study was to investigate the mechanisms underpinning depressive behavior in the context of ALSP, utilizing microglial-specific Csf1r haploinsufficient mice. Our findings indicate that these mice exhibited depressive-like behaviors, as well as microglial hyper-ramification and aberrant synaptic pruning capacity.

Inhibiting Staphylococcus aureus Virulence Factors: Advances in Traditional Chinese Medicines and Active Compounds.

Staphylococcus aureus is one of the most prevalent antibiotic-resistant bacteria, characterized by high morbidity and mortality. The pathogenicity of S. aureus relies on the production of multiple virulence factors.

Internet addiction and its association with quality of life in college students: a network perspective.

Background: Internet addiction (IA), especially in young people, has gained increasing attention in recent years. This study examined the prevalence of IA and its associated factors, relationship with quality of life (QoL) and network structure among college students.

Methods: A cross-sectional study was conducted between September and December 2023 in China.

Huang-Lian-Jie-Du decoction alleviates cognitive deficits in Alzheimer's disease model 5xFAD mice by inhibiting Trem2/Dap12 signaling pathway.

Background: Alzheimer's disease (AD) is a progressive neurodegenerative disorder predominantly affecting the elderly population. It is characterized by cognitive deficits associated with the accumulation of amyloid-beta plaques and neurofibrillary tangles. Huang-Lian-Jie-Du (HLJD) decoction, recognized as a representative formulation with heat-clearing and detoxification effects, has been demonstrated to be effective in treating AD.

MRAgent: an LLM-based automated agent for causal knowledge discovery in disease via Mendelian randomization.

Understanding causality in medical research is essential for developing effective interventions and diagnostic tools. Mendelian Randomization (MR) is a pivotal method for inferring causality through genetic data. However, MR analysis often requires pre-identification of exposure-outcome pairs from clinical experience or literature, which can be challenging to obtain.

2D Benzothiophene-Based Covalent Organic Frameworks for Efficient Iodine Capture.

The leakage of iodine and its radioactive isotopes poses a major threat to ecosystems and human health, emphasizing the need for effective and recyclable iodine capture materials. Covalent organic frameworks (COFs), characterized by high surface areas, tunable pore sizes, and excellent chemical stability, are ideal candidates for adsorption applications. Herein, two novel COFs (JUC-700 and JUC-701) are designed and synthesized based on benzotrithiophene (BTT).

Recent insights on the impact of SWELL1 on metabolic syndromes.

SWELL1 is a key component of the volume-regulated anion channel (VRAC) and participates in cell volume regulation as an ion channel plasma membrane protein. While early studies focused on its role in immune cell development and tumor progression, recent studies have revealed that SWELL1 plays an important role in metabolic diseases. Studies have shown that SWELL1 is extensively involved in physiological processes in peripheral metabolic tissues, including adipocyte hypertrophy, skeletal muscle volume regulation, insulin secretion, and hepatic lipid metabolism through interactions with the insulin signaling pathway.

The effect of electric field microenvironment on the exosome secretion of PC12 cells by chitosan carbon dots.

When neurons are sufficiently stimulated, they generate electrical signals with rapid voltage changes, and information is transferred between neurons through neurites. Chitosan carbon dots (CS CDs) possess unique optical properties, excellent biocompatibility, and superior electron transfer capabilities, making them promising materials for nerve tissue repair and replacement. Studies have shown that exosome (EXO) secretion by PC12 cells and neurite formation are critical for neural tissue repair.

Boosting work engagement through leader tolerance: the chain mediation role of perceived organizational support and organizational identification.

Introduction: To enhance competitiveness, numerous organizations have introduced control and penalty systems to manage employee work errors. However, these systems have often backfired, negatively impacting employees' emotions and behaviors. Recognizing the critical role of leadership in error management, this study examines how leaders' tolerance of their followers' mistakes influences employees' work engagement, drawing on Affective Events Theory (AET).

Effects of simulated digestion and prebiotics properties of polysaccharides extracted from based on different pilot processes.

Recent studies have highlighted the prebiotic potential of natural plant polysaccharides, demonstrating their role in promoting beneficial gut microbiota and improving health. However, research on the digestive properties and prebiotic activities of (IRPs) remains limited. This study investigated fresh as the research object.

Gender difference in prevalence and network structure of subclinical Hikikomori and depression among college students.

Background: Subclinical Hikikomori and depression are common among college students, yet gender differences in their prevalence and interrelationships are under-explored. This study evaluated gender differences in prevalence and symptom networks of these disturbances.

Methods: A large-scale, multi-center study was conducted across Xinjiang, Qinghai, and Guangdong provinces, China between September and December 2023.

DSS-YOLO: an improved lightweight real-time fire detection model based on YOLOv8.

Fire disasters pose significant risks to human life, economic development, and social stability. The early stages of a fire, often characterized by small flames, diffuse smoke, and obstructed objects, can lead to challenges such as missed detections and poor real-time performance. To address these issues, we propose a DSS-YOLO model based on an improved YOLOv8n architecture, designed to enhance the recognition accuracy of obscured objects and small targets while reducing computational overhead.

Enhanced electrochemical detection of dopamine and uric acid using Au@Ni-MOF and employing 2D structure DFT simulation.

The accurate and expeditious detection of minute biomolecules within human body fluids holds paramount significance in the advancement of novel electrode materials. In this research, a novel non-enzyme electrochemical sensor was constructed. It was founded on Au@Ni-MOF (Ni(CHCO)) hybrids, with Ni(II) (nickel acetate) serving as the precursor.

Preparation and adsorption properties of microsphere geopolymers derived from calcium carbide slag and fly ash.

In this work, using fly ash and calcium carbide slag as mixed precursors, hydrogen peroxide and sodium dodecyl sulfate as mixed foaming agents, porous microsphere geopolymer was prepared by suspension curing technology to remove Cu (II) from aqueous solution. Because the Ca (OH) in calcium carbide slag effectively improves the pozzolana activity of fly ash, more C-S-H gel is produced in the geopolymerization process, and the foaming process makes the material have larger specific surface area and better adsorption properties. The results show that the specific surface area of the material is 63.