Single-cell sequencing uncovers disrupted stromal-macrophage communication as a driver of intrauterine adhesion progression.

Intrauterine adhesions (IUA), characterized by endometrial fibrosis, pose a serious threat to women's reproductive health, yet their molecular mechanisms remain poorly understood. Here, we use single-cell RNA sequencing (scRNA-seq) to profile 139,395 single cells from nine individuals in the proliferative phase. We identify seven stromal and five macrophage subsets, revealing increased immune cell infiltration and a profibrotic shift in macrophage states.

25-hydroxyvitamin D3 exerts a protective effect on coronary artery lesions in a mouse model of Kawasaki disease by inhibiting JAK1/STAT3 and TLR4 pathways.

Background: Kawasaki disease (KD) is an acute vasculitis in children, and the resultant inflammatory process can lead to coronary artery aneurysms. The study aimed to investigate the role of 25-hydroxyvitamin D (25-OH-D), a stable circulating form of vitamin D, in KD mouse models.

Methods: The KD mouse model was established through intraperitoneal injection of 500 μg cell wall extract (LCWE).

A novel EDA variant that causes X-linked hypohidrotic ectodermal dysplasia in a Chinese family.

Background: Hypohidrotic ectodermal dysplasia (HED) is a rare genetic disorder that affects the development of the skin, hair, nails, teeth, and sweat glands. Due to its rarity, there are currently few methods that can be applied to facilitate its diagnosis during the prenatal period. Although a prenatal ultrasonographic examination will detect early signs of the disease, there are few reports on specific prenatal ultrasonographic features of ectodermal dysplasia.

[Invasive resulting in severe tracheal stenosis:a case report].

Invasive (ITBA) is a relatively rare manifestation of invasive pulmonary aspergillosis(IPA), which usually occurrs in immunocompromised patients and with a fatal outcome. Here, we reported a 37-year-old immunocompetent male patient with psoriasis who was hospitalized for dyspnea. Bronchoscopy revealed pseudomembranous tracheobronchitis, and ITBA was subsequently confirmed pathologically.

Phospholipase Cε1 activates Rap1 signaling pathway to promote proliferation, EMT and angiogenesis of choriocarcinoma cells.

Background: Choriocarcinoma is a rare yet highly malignant gestational trophoblastic tumor, and its molecular mechanisms remain largely unclear. This study reveals the critical role of phospholipase Cε1 (PLCE1) in the malignant progression of choriocarcinoma and its epigenetic regulatory mechanisms.

Methods: The GSE88873 dataset was analyzed to assess PLCE1 expression in choriocarcinoma.

Combined nomogram for differentiating adrenal pheochromocytoma from large-diameter lipid-poor adenoma using multiphase CT radiomics and clinico-radiological features.

Background And Objective: Adrenal incidentalomas (AIs) are predominantly adrenal adenomas (80%), with a smaller proportion (7%) being pheochromocytomas(PHEO). Adenomas are typically non-functional tumors managed through observation or medication, with some cases requiring surgical removal, which is generally safe. In contrast, PHEO secrete catecholamines, causing severe blood pressure fluctuations, making surgical resection the only treatment option.

Real-world single-center data analysis of dual immunotherapy in advanced NSCLC: Efficacy, survival, and adverse events.

We collected 96 patients who received dual immunotherapy between January 2019 and June 2024, in a 1:1:1 ratio based on PD-L1 expression levels of negative (<1%), medium (1% ≤ PD-L1 ≤ 49%), and high (PD-L1 ≥ 50%). This study evaluates the efficacy and safety of dual immunotherapy in patients with advanced NSCLC, stratified by PD-L1 expression levels. Among the 96 patients, the objective response rate (ORR) was 37.

ABCA4 regulates mitophagy in lung adenocarcinoma progression via AMPK pathway by modulating TMSB4X.

Background: Lung adenocarcinoma (LUAD), the most common subtype of lung cancer, has an unfavorable prognosis. ABCA4 has been identified as an oncogene in multiple malignancies, but its specific mechanisms in LUAD remain poorly understood. This study aims to explore the function of ABCA4 in LUAD with a focus on mitophagy.

Quantifying the Trajectory of Percutaneous Endoscopic Lumbar Discectomy in 3D Lumbar Models Based on Automated MR Image Segmentation-A Cross-Sectional Study.

Objective: Creating a 3D lumbar model and planning a personalized puncture trajectory has an advantage in establishing the working channel for percutaneous endoscopic lumbar discectomy (PELD). However, existing 3D lumbar models, which seldom include lumbar nerves and dural sac reconstructions, primarily depend on CT images for preoperative trajectory planning. Therefore, our study aims to further investigate the relationship between different virtual working channels and the 3D lumbar model, which includes automated MR image segmentation of lumbar bone, nerves, and dural sac at the L4/L5 level.

Mechanisms of cell senescence and apoptosis in cyclophosphamide-induced premature ovarian failure in rats.

Background: Premature ovarian failure (POF) is a clinical condition characterized by a diminished ovarian reserve occurring before the age of 40, significantly affecting female reproductive health. However, its exact pathogenesis remains unclear. This research aimed to examine the mechanisms of cyclophosphamide (CTX)-induced senescence and apoptosis in the ovarian and cerebral cortex tissues of rats to provide insights into delaying aging and protecting female reproductive health.

TSC2-mutated perivascular epithelioid cell tumor with partial response to mTOR inhibition: a case report and literature review.

Perivascular epithelioid cell tumors (PEComas), rare mesenchymal neoplasms with heterogeneous behavior, are molecularly characterized by TSC2 inactivation driving mammalian target of rapamycin (mTOR) pathway activation. We present a typical case of a 63-year-old female with metastatic high-grade PEComa featuring a TSC2 mutation (68.57% VAF) and elevated tumor mutational burden (19.

Mapping the Cross-species Brain Connectivity Atlas and Hemispheric Asymmetry of the Temporal Pole in Humans and Macaques.

The temporal pole (TP), one of the most expanded cortical regions in humans relative to other primates, plays a crucial role in human language processing. It is also one of the most structurally and functionally asymmetric regions. However, whether the functional architecture of the TP is shared by humans and macaques is an open question.

Inhibition of GPR75 Alleviates Lipid Metabolism by Activating the AMPK-SIRT1 Signaling Pathway In Vitro and In Vivo.

The pathogenesis of non-alcoholic fatty liver disease (NAFLD) is primarily driven by excessive lipid accumulation and metabolic dysregulation, necessitating a comprehensive investigation into the underlying mechanisms. This study employed an in vitro model, wherein Huh7 cells were induced with a palmitic acid/oleic acid mixture, and an in vivo model involving the provision of a high-fat diet to SD rats for six weeks. Employing techniques such as oil red O staining, immunofluorescence, and Western blotting, we examined lipid synthesis, metabolism, and the associated molecular pathways.

CEBPB activates NRF2 to regulate the MAPK pathway through DUSP1 to promote proliferation and antioxidant capacity in ovarian cancer cells.

NRF2, a crucial antioxidant transcription factor in ovarian cancer (OC), is closely associated with CEBPB activation. However, the regulatory mechanism of NRF2 by CEBPB in OC remains poorly understood. In this study, we systematically evaluated the malignant behavior of SKOV3 and A2780 cells through comprehensive approaches, including CCK-8 kits, clone formation assays, and flow cytometry analysis.

Impact of gut microbiota on endometriosis: linking physical injury to mental health.

Endometriosis is widely recognized as a chronic, inflammatory, and heterogeneous condition that can manifest in various anatomical locations within females. It is marked by estrogen-driven cycles of bleeding, cell proliferation, and fibrosis involving ectopic endometrial glands and stroma cells located outside the uterus. The limited understanding of its etiology and complex pathogenesis has created obstacles in achieving early diagnosis and developing effective treatments with minimal side effects.

Development of a KRP-based pH-responsive drug delivery system for solid tumors.

Background: Cell-penetrating peptides (CPPs) are considered ideal carriers for the delivery of drugs through the cell membrane barrier, with enhanced permeation and retention effects. These peptides can increase the bioavailability of drugs and reduce their side effects. In this study, we developed a polypeptide called "cell-penetrating peptide (KRP)", linked it with doxorubicin (DOX), and created a pH-responsive triggered drug delivery system called "KRP-Hyd-DOX".

Diagnostic performance of magnetic resonance imaging for lateral pelvic lymph node metastasis in patients with rectal carcinoma: a meta-analysis and systematic review.

Purpose: Accurate identification of lateral pelvic lymph node (LPLN) metastasis is imperative for guiding LPLN dissection to reduce local recurrence in patients with rectal carcinoma. This meta-analysis aimed to investigate the diagnostic performance of magnetic resonance imaging (MRI) for LPLN metastasis in patients with rectal carcinoma.

Methods: Embase, PubMed, Web of Science, and the Cochrane Library were searched to identify studies related to the diagnostic performance of MRI for LPLN metastasis in patients with rectal carcinoma through June 2024.

Kasumi-1 exosome plays a major T-cell immune evasion role in TP53-type acute leukemia.

Background: The treatment and prognosis for TP53-mutant acute leukemia (AL) are notably unfavorable. Tumor-derived exosomes are participating in tumorigenesis and immunomodulation. Our objective was to characterize the exosome-mediated immune landscape in TP53-mutant AL.

Irisin regulates integrin αvβ5/FAK/ERK to inhibit neutrophil extracellular traps formation and reduce pancreatic beta-cells pyroptosis in type 2 diabetes mellitus.

Background: Dysregulation of neutrophil extracellular traps (NETs), through overproduction or poor clearance, can lead to tissue damage and is linked to inflammatory conditions like type 2 diabetes mellitus (T2DM). Irisin, a hormone believed to modulate energy metabolism and enhance insulin sensitivity, holds promise as a potential treatment for T2DM. This study aims to investigate the role of Irisin in alleviating β-cell pyroptosis by inhibiting NETs formation and elucidating the underlying molecular mechanisms.

Targeting the METTL3/YTHDF3/m6A/PGK1 axis to combat Choriocarcinoma progression.

Choriocarcinoma (CC) is a highly aggressive trophoblastic tumor characterized by rapid progression and early metastasis. Glycolysis, a crucial metabolic pathway in cancer, is essential for tumor growth, yet its specific role in CC is not well understood. Phosphoglycerate kinase 1 (PGK1), an important enzyme in glycolysis, along with N6-methyladenosine (m6A) RNA methylation, a common modification of mRNA, are both associated with tumorigenesis.

Topography-guided subconjunctival oblique limbus incisions (SCOLI) in manual cataract surgery for astigmatism correction.

Purpose: To assess the safety and efficacy of two SCOLI designs (Yang's I/II) for astigmatism correction in manual cataract surgery and define incision size-astigmatism relationships.

Methods: A total of 72 eyes from 64 cataract patients who accept manual cataract extraction surgery via a SCOLI technique. The two different types of incision: Yang's I and II incisions were applied in this study.

Clinical features and risk factors for severe macrolide-resistant mycoplasma pneumoniae pneumonia induced by 23 S rRNA A2063G mutation: a retrospective observational study.

Objectives: This study aimed to investigate the clinical characteristics and risk factors of severe macrolide-resistant Mycoplasma pneumoniae pneumonia (MRMP) induced by the 23 S rRNA A2063G mutation.

Methods: Clinical data were collected from 526 pediatric patients diagnosed with Mycoplasma pneumoniae pneumonia (MPP) at Kunming Children's Hospital, representing a single-center cohort study conducted between October 2023 and February 2024. Among them, 483 cases (91.

The Evolutionary Trajectory and Prognostic Value of GITR+ Tregs Reprogramed by Tumor-Intrinsic PD-1/c-MET Signaling in Pancreatic Cancer.

Tumor-intrinsic programmed cell death 1 (PD-1) has been shown to activate the mesenchymal epithelial transition factor (MET) pathway via its phosphorylation in pancreatic ductal adenocarcinoma (PDAC). However, the immunoregulatory consequences of MET activation remain poorly understood. Herein, a significant positive correlation between phosphorylated MET (p-MET) and tumor-intrinsic PD-1 is verified, both of which are independently associated with adverse prognosis.

Case Report: Two children with factor XII deficiency caused by novel compound heterozygous variants.

Background: Factor XII (FXII) deficiency (OMIM 234000) is a rare hereditary coagulation disorder caused by pathogenic variants within the gene. It causes prolonged activated partial thromboplastin time without bleeding diathesis. Most patients have no obvious clinical symptoms, so the disease is difficult to be detected.

D-dimer predicts the response of patients with gastric cancer to first-line immunotherapy combined with chemotherapy.

Background: Immune checkpoint inhibitors (ICIs) combined with chemotherapy have emerged as a new choice for advanced and metastatic gastric cancer (GC) patients. Due to the lack of unified and effective predictive biomarkers, there is an urgent need to find accurate biomarkers. The aim of our study was to explore the prognostic value of the pretreatment D-dimer levels on the effect of immunotherapy combined with chemotherapy in advanced and metastatic GC patients.