An ELISA-like sensitive and visual detection system targeting based on CRISPR/Cas12a and DNAzyme.

is the causative agent of plague, a human disease with potentially devastating consequences. Here, we developed an enzyme-linked immunosorbent assay-like visual detection method based on clustered regularly interspaced short palindromic repeats (CRISPR) detection and DNAzyme for the cost-effective and highly sensitive detection of . A novel specific gene sequence (CH57_3927) was screened for the detection target of .

Contributions of different host species to the natural transmission of severe fever with thrombocytopenia syndrome virus in China.

Severe fever with thrombocytopenia syndrome (SFTS) is a tick-borne emerging infectious disease with a reported mortality rate of up to 30% in hospitalized patients. The causative agent, SFTS virus (SFTSV) is maintained in nature through a transmission cycle involving animal hosts and ticks. Therefore, effective control of SFTS in nature environments necessitates a comprehensive understanding of the tick-host circulation patterns that sustain viral persistence.

Benefits of public awareness in mitigating cystic echinococcosis risk in Western China: A climate and socio-economic perspective.

Background: The prevalence of cystic echinococcosis (CE), a widespread zoonotic disease, imposes a significant public health burden, especially in western China. However, under the background of global change, how to meet the challenge of the future risk of CE remains unclear. As global climate change, land use changes, and socio-economic factors continue to progress, the spread and intensity of CE may potentially worsen, making it crucial to assess and mitigate future risks.

Mapping HIV-1 RNA structure, homodimers, long-range interactions and persistent domains by HiCapR.

Human Immunodeficiency Virus type 1 (HIV-1) RNA genome organization remains a critical knowledge gap in understanding its replication cycle. To address this, we developed HiCapR, a psoralen crosslinking-based RNA proximity ligation method coupled with post-library hybridization, enabling high-resolution mapping of RNA-RNA interactions across the HIV-1 genome. This approach confirmed canonical structural motifs, including stem-loop architectures in the 5'-untranslated region (5'-UTR) and Rev Response Element (RRE), as well as dimerization sites within the 5'-UTR critical for viral packaging.

Babesia microti (Babesiidae, Piroplasmida) infection in a Chinese traveler returning from the United States of America.

Background: Human babesiosis, caused by intraerythrocytic protozoa of the genus Babesia (Piroplasmida, Babesiidae), is a globally emerging zoonosis transmitted primarily through Ixodes spp. ticks. Babesia microti, which is endemic particularly in the northeastern and midwestern United States, accounts for the majority of globally reported human cases.

Knowledge, attitudes, and practices toward ticks and tick-borne diseases: a cross-sectional study in Rwanda.

Background: Ticks and tick-borne diseases pose significant global risks to humans and animals, leading to economic losses and health threats. This study aimed to assess the knowledge, attitudes, and practices toward ticks and tick-borne diseases among adults in Rwanda.

Methods: The sample size was determined using a single-population proportion formula.

Geospatial and modelling analyses reveal diverse tick and tick-associated microbes in the East African Community.

Background: The continuous geographic expansion of ticks and the emergence of tick-borne diseases have raised tremendous global public health concerns, particularly in the East African Community (EAC). This study aimed to investigate the distribution of ticks and tick-associated microbes and to predict the potential extension of dominant tick species in the EAC.

Methods: Data were collected from literature reviews and related websites and analyzed using ArcGIS to generate maps showing the geographical distribution of ticks and associated microbes.

ANG-Modified Liposomes Coloaded With α-Melittin and Resveratrol Induce Apoptosis and Pyroptosis in Glioblastoma Cells by Impeding Wnt/β-Catenin Signaling.

Main Problem: Glioblastoma (GB) is one of the most prevalent and devastating types of brain cancer for which efficient treatments are currently lacking because of limitations such as antitumor efficacy, brain delivery, tumor selectivity, and drug resistance. A promising strategy to overcome these obstacles is developing anticancer agents that can be delivered to GB tissues to inhibit tumors with low toxicity to normal brain tissue.

Methods: We developed liposomes encapsulating resveratrol (RES), a polyphenolic compound, and α-melittin (α-MEL), which is composed of melittin conjugated with an amphiphilic α-helical peptide at its N-terminus.

Characteristics and Antibacterial Activity of Staphylococcus aureus Phage-Derived Endolysin LysP4.

The rise in multidrug resistance and strong biofilm-forming ability of Staphylococcus aureus has led to significant public health concerns. Phage or phage-derived components, such as depolymerase or endolysin, have been considered as potential alternatives to antibiotics for combating antibiotic-resistant bacterial infections. In this study, we cloned and expressed a Staphylococcus aureus phage endolysin, LysP4, and identified its lytic activity.

Aromatic Molecular Compatibility Attenuates Influenza Virus-Induced Acute Lung Injury via the Lung-Gut Axis and Lipid Droplet Modulation.

Acute lung injury (ALI) is a major cause of death in patients with various viral pneumonias. Our team previously identified four volatile compounds from aromatic Chinese medicines. Based on molecular compatibility theory, we defined their combination as aromatic molecular compatibility (AC), though its therapeutic effects and underlying mechanisms remain unclear.

Molecular Mechanism of Aflatoxin B Synthesis Related AfVerB Regulating the Development, AFB Biosyntheis and Virulence of Mainly Through Its CYP Domain.

and its secondary metabolites aflatoxins pose a significant threat to the health of humans, animals, and plants. Therefore, there is an urgent need to control contamination. AfverB plays a key role in the aflatoxin gene cluster; however, its function and mechanism in fungal development and virulence remain poorly understood.

Spatiotemporal distribution and influencing factors of enteric fever in China: a cluster analysis based on data from 2001 to 2020.

Background: Enteric fever primarily affects the southwestern and central regions of China. Although the overall incidence rate has declined, certain areas have seen an increase in cases, necessitating further investigation into their geographic distribution, clustering areas, and potential influencing factors.

Methods: City-level data from 2001 to 2020 were analyzed.

Not for nothing, microplastics can (potentially) reduce the risk of mosquito-to-human transmission of arboviruses.

The impact of microplastic pollution has emerged as a significant global ecological concern. Various organisms have exhibited alterations in behavior or metabolic activities following exposure to microplastics (MPs). Mosquitoes, as crucial disease vectors, are particularly susceptible MPs exposure in the environment.

Development of a neutralization assay and bioluminescent imaging mouse model for Dehong virus (DEHV) using a pseudovirus system.

Dehong virus (DEHV) is an emerging filamentous virus of considerable interest. However, research involving DEHV remains limited, and no suitable models exist to investigate its pathogenicity or transmission. In this study, we developed an neutralization assay to detect DEHV-neutralizing antibodies, as well as an bioluminescent imaging mouse model based on a pseudovirus system.

Transcriptome analysis of midgut and salivary gland post-Zika virus infection.

This study aimed to investigate the transcriptomic changes in the midgut and salivary glands of mosquitoes infected with Zika virus (ZIKV), in order to explore the molecular mechanisms underlying the interaction between the virus and the mosquito vector. from Jiegao (JG) and Mengding (MD) in China were experimentally infected with ZIKV, and the midgut and salivary gland tissues were collected at 2-, 4- and 6 days post-infection (dpi). High-throughput sequencing was performed to analyze the transcriptomic changes between ZIKV-infected and non-infected .

Virome diversity and potential sharing of wild mammals in a biodiversity hotspot, Yunnan, China.

Background: Small mammals, including rodents, shrews and moonrats are widespread and serve as natural reservoirs for many viral pathogens. However, the composition and distribution of wild animal viromes remain poorly understood. At least 10,000 virus species have the ability to infect humans, but the vast majority are circulating silently in wild mammals.

Towards the first synthetic eukaryotic cell.

With the rapid advance in synthetic biology and the expanding field of synthetic genomics, the realization of a redesigned yeast genome has become an achievable milestone. Multiple eukaryotic chromosomes, meticulously designed and synthesized, are now being systematically integrated to create an entirely synthetic eukaryotic cell. This comprehensive review examines the fundamental design principles and construction strategies, highlighting critical technological breakthroughs in pursuing the first synthetic eukaryotic cell.

Cyberbiosecurity: Advancements in DNA-based information security.

Synthetic biology is a crucial component of the "cyber-biological revolution" in this new industrial revolution. Owing to breakthroughs in synthetic biology, deoxyribonucleic acid (DNA), the storehouse of hereditary material in biological systems, can now be used as a medium for storage (synthesis) and reading (sequencing) of information. However, integrating synthetic biology with computerization has also caused cyberbiosecurity concerns, encompassing biosecurity and information security issues.

Assessing the global dengue burden: Incidence, mortality, and disability trends over three decades.

Background: Dengue, the fastest-spreading vector-borne disease (VBD), significantly burdens global health systems. This study analyzed the trends in the global burden of dengue from 1990 to 2021, utilizing data from the Global Burden of Diseases, Injuries, and Risk Factors Study 2021 (GBD 2021).

Methodology/principal Findings: We retrieved data from GBD 2021 regarding dengue, including the number of incidences and age-standardized incidence rate (ASIR), the number of deaths and age-standardized death rate (ASDR), disability-adjusted life-years (DALYs) and age-standardized DALYs.

Development and evaluation of the digital PCR-based method for clinical monitoring of viral loads during severe fever with thrombocytopenia syndrome virus infection.

Background: Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) represents a novel bunyavirus that poses significant public health challenges. As a key prognostic indicator of clinical outcome, the viral load determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is relatively inaccurate and incomparable across different studies. Digital PCR (dPCR) has recently proved to be a more ideal tool for viral load assessment.

Transient receptor potential vanilloid-1 (TRPV1) is a mediator of noise-induced neural damage in zebrafish and mice.

Sound pollution (noise) is an increasing environmental concern, particularly associated with neurological and neurobehavioral abnormalities. However, the molecular mechanisms underlying noise-induced neural damage remain unclear. In this study, we conducted transcriptional profiling of zebrafish to investigate the mechanisms underlying acoustic stimulation (1,000 Hz, 130 dB).

Estimated size of the total genome and protein space of viruses.

Recent metagenomic studies have identified a vast number of viruses. However, the systematic assessment of the true genetic diversity of the whole virus community on our planet remains to be investigated. Here, we explored the genome and protein space of viruses by simulating the process of virus discovery in viral metagenomic studies.

Near-Full-Length Genomic Characterization of Two Novel HIV-1 Unique Recombinants (CRF01_AE/CRF07_BC) and (CRF01_AE/CRF68_01B) in Shijiazhuang, Hebei Province, China.

Heterosexual transmission (HETE) represents the predominant method of transmission for the human immunodeficiency virus type 1 (HIV-1) in Shijiazhuang, Hebei Province, China. The number of circulating recombinant forms (CRFs) and unique recombinant forms (URFs) continues to increase in this region. In the present study, two novel URFs (TFH010919 and TFH010944) were identified, both derived from HETEs in the Shijiazhuang area.

Role of CCL2/CCR2 axis in pulmonary fibrosis induced by respiratory viruses.

Respiratory virus infection is an important cause of both community acquired pneumonia and hospital-acquired pneumonia. Various respiratory viruses, including influenza virus, avian influenza virus, respiratory syncytial virus (RSV), SARS-CoV, MERS-CoV, and SARS-CoV-2, result in severe fibrosis sequelae after the acute phase. Since the COVID-19 pandemic, respiratory virus infection, as an important cause of pulmonary fibrosis, has attracted increasing attention around the world.