Pacific Oyster-Derived Alcohol Dehydrogenase Activating Peptide Leu-Gln-Pro-Pro-Arg Ameliorates Alcoholic Liver Disease by Enhancing Alcohol Metabolism and Suppressing Mitochondrial-Mediated Apoptosis.

Alcohol dehydrogenase (ADH) plays an important role in improving alcohol metabolism. Bioactive peptides, as functional factors with low toxicity and easy absorption, have attracted increasing attention in the intervention of alcoholic liver disease (ALD). This study investigated the effects of LQPPR, an ADH-activating peptide from Pacific oysters, on ALD and further elucidated its mechanism.

Insights into volatile substance changes of tilapia fillets before and after steaming and boiling during refrigeration based on metabolomics.

This study investigated the key volatile substances using SPME-GC-MS and metabolites using LC-MS, as tilapia fillets before and after steaming and boiling during refrigeration. Results indicated untreated, steamed, and boiled tilapia fillets post-refrigeration exhibited 15, 22, and 23 key volatile substances, respectively, primarily aldehydes, alcohols, and ketones. 97, 70, and 67 differential metabolites were identified by PLS-DA, respectively.

Pulp Residues Polysaccharide Alleviates High-Fat Diet-Induced Obesity by Modulating Intestinal Mucus Secretion and Glycosylation.

Although pulp residues polysaccharides (HPPP) have shown potential in improving metabolic disorders and intestinal barrier function, the mechanism by which they exert their effects through regulating O-glycosylation modifications in the mucus layer remains unclear. Therefore, this study established a HFD-induced obese colitis mouse model ( = 5 per group) and combined nano-capillary liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) technology to quantitatively analyze the dynamic changes in O-glycosylation. Additionally, through quantitative O-glycosylation proteomics and whole-proteome analysis, we identified 155 specifically altered O-glycosylation sites in colon tissue, with the glycosylation modification level of the MUC2 core protein increased by approximately 2.

Supramolecular Co-Assembled Fmoc-FRGDF/Hyaluronic Acid Hydrogel for Quercetin Delivery: Multifunctional Bioactive Platform.

During food processing and storage, traditional protein-based delivery systems encounter significant challenges in maintaining the structural and functional integrity of bioactive compounds, primarily due to their temporal instability. In this study, a nanocomposite hydrogel was prepared through the co-assembly of a self-assembling peptide, 9-Fluorenylmethoxycarbonyl-phenylalanine-arginine-glycine-aspartic acid-phenylalanine (Fmoc-FRGDF), and hyaluronic acid (HA). The stability of this hydrogel as a quercetin (Que) delivery carrier was systematically investigated.

A selenium-enriched glycosaminoglycan from sturgeon cartilage: characterization and anti-metabolic syndrome potential.

This study develops a novel selenium-enriched chondroitin sulfate (CSSE) from selenium-rich sturgeon cartilage that has potent multitarget activity against high-carbohydrate/high-fat diet-induced metabolic syndrome. Structural analyses (NMR/MALDI-TOF MS) confirmed Se-O-SO₃ covalent bonds (δ 78.5 ppm in C NMR) and a molecular weight of 16.

Anserine alleviates atherosclerosis in ApoE mice by regulating lipid metabolism.

Background: Anserine is a bioactive and nutritional food supplement, widely used in the food health supplement, athletic nutrition, and medical fields. The aim was to investigate the mechanism of anserine in alleviating atherosclerosis. ApoE mice were fed a high-fat diet and orally administered anserine at doses of 30, 60, and 120 mg kg d for 16 weeks.

pH modulates grass carp myofibrillar protein-aldehyde interactions: Molecular mechanistic insights.

The interactions between grass carp myofibrillar proteins (MPs) and selected aldehydes under different pH conditions were analyzed. The results showed that the binding capacity of MPs to the aldehydes followed the order: nonanal > octanal > hexanal, and their binding capacity increased with rising pH (5-9). MPs-aldehyde interactions exhibited static and dynamic quenching, predominantly mediated by hydrophobic forces, with pH exerting no effect on the quenching mode.

Insights into the comprehensive structural characterization of fish oil-modified starch and its influence on surimi product quality.

To address the quality degradation in surimi products due to lipid loss, the oil-modified starch made by amylose corn starch and fish oil was used to investigate the comprehensive structural characterization of fish oil-modified starch and its influence on surimi product quality. The results indicated that hydrothermal treatment temperature played a key role in the successful preparation of fish oil modified starch. At a modification temperature of 90 °C, the CI of fish oil-modified starch reached a maximum of 59.

Insights into the Mechanism Underpinning Composite Molecular Docking During the Self-Assembly of Fucoidan Biopolymers with Peptide Nanofibrils.

Composite hydrogels with improved mechanical and chemical properties can be formed by non-covalently decorating the nanofibrillar structures formed by the self-assembly of peptides with fucoidan. Nevertheless, the precise interactions, and the electrochemical and thermodynamic stability of these composite materials have not been determined. Here, we present a thermodynamic analysis of the interacting forces that drive the formation of a composite fucoidan/9-fluorenylmethoxycarbonyl-phenylalanine-arginine-glycine-aspartic acid-phenylalanine (Fmoc-FRGDF) hydrogel.

Effect of water redistribution on the rheological behavior of MP-PSs and 3D printability of surimi: An investigation using LF-NMR.

This study explored the effect of interaction between starch and water on 3D printing adaptability of surimi, a topic that has received limited attention in previous research. The 3D printability of surimi-starch systems was examined under controlled moisture conditions, and notable enhancements in printing quality were observed when starch concentrations were adjusted to 8-12 % (with a fixed water content of 70 %) or reduced to 4 % with a corresponding increase in water to 80 %. To gain further insights, we investigated myofibrillar protein-potato starch blend system and manipulated the proportions of the constituents and water content, using LF-NMR technology to analyze the competitive adsorption of water molecules among the components.

Environmental factors and blueberry anthocyanin-induced conformational changes modulate the interaction between myofibrillar proteins and fishy compounds and their mechanism, specifically aldehydes and alcohols.

This study investigated the impact of blueberry anthocyanin (BA) on the interaction between tilapia myofibrillar protein (MP) and fishy compounds (hexanal, octanal, nonanal, trans-2-nonenal, and 1-octen-3-ol). Results indicated that at a protein concentration of 5 mg/mL and fishy compounds at 5 μg/mL, MP effectively adsorbed these compounds at 4 °C, pH 7.0, and 0.

Mitigating Effect of Taurine Combined with Corona Dormancy on Oxidative Stress in Under Low-Temperature Stress.

The purpose of the present work was to establish the transportation conditions for keeping alive by means of corona-induced dormancy in combination with taurine. It also investigated the synergistic regulatory effects on oxidative stress mitigation and immune function during low-temperature conditions and clarified the underlying mechanism. The dormancy pretreatment induced by pulsed direct current could reduce the accumulation of reactive oxygen species in fish under hypothermal and water-restricted conditions and significantly enhance the environmental adaptability of .

A Comparative Study on Physicochemical Properties and Biological Activities of Polysaccharides from Buds Obtained by Different Methods.

In this study, the polysaccharides of buds (CTBPs) were extracted by hot water, ultrasound, alkali solution, and acid solution, and the four kinds of extracted polysaccharides were denoted Hw, Ultra, Al, and Ac. Then, the Hw were degraded by ultrasound, an alkali solution, and an acid solution, and the three resultant kinds of polysaccharides were denoted Ultra-Post-proc, Al-Post-proc, and Ac-Post-proc. The study comprehensively compared and analyzed the physical and chemical characteristics, structural properties, and in vitro activities of each polysaccharide.

Investigating the relationship between fish oil oleogelation and gelation characteristics: Comparison of structure and function in pork and golden pompano (Trachinotus ovatus) egg box-like protein network.

Oleogels can replace animal fat and improve quality in gelled products. The effect of their structure characteristics on gelation mechanism was investigated from the multi-scale, and comparison of spatial structure and function from pork and golden pompano (Trachinotus ovatus) proteins was clarified. The results showed that increasing beeswax concentration facilitated molecular assembly and network structure, thus improving oil/water-holding capacity in fish and pork protein gels.

Effect of dense phase carbon dioxide on the digestive properties of shrimp surimi gels: Insight from digestive kinetic.

Understanding the protein digestion rate and the release of nutrients in the gastrointestinal tract is critical to designing novel food products with enhanced functionalities. The digestion characteristics of untreated shrimp surimi and surimi gels induced by water-bath heating (WB) and different dense phase carbon dioxide (DPCD) treatment (L, M, and H crosslink degree) groups were studied. With the increasing crosslink, the protein digestibility increased and the average particle size decreased.

Dietary polysaccharides from dragon fruit pomace, a co-product of the fruit processing industry, exhibit therapeutic potential in high-fat diet-induced metabolic disorders.

The increasing prevalence of metabolic disorders, often associated with high-fat diets (HFD), highlights the need for new therapeutic approaches, especially from natural sources. Dragon fruit pomace, a by-product of the fruit processing industry, is rich in polysaccharides with potential health benefits. This study investigates the effects of dragon fruit pomace-derived polysaccharides (PDPS) in alleviating HFD-induced metabolic dysfunction.

Effect of high-voltage electrostatic field salting on the quality of salt-reduced Yi ye Cheng golden pomfret.

This study investigates the effect of high-voltage electrostatic field (HVEF) salting on the quality of salt-reduced Yi Ye Cheng golden pomfret. Results showed that the salt content increased with the voltage ( < 0.05).

The regulation of cassava nanostarch structure treated with malt endogenous enzymes and adsorption property for anthocyanins.

The enzymatic preparation of nanostarch exhibits specific and efficient. However, commercial amylases face issues of expensive and time-consuming extraction, which limit their application. This study obtained cassava nanostarch (MCS) from Cassava starch (CS) treated with malt endogenous amylase, followed by structural characterization and property exploration.

Quercetin-functionalized nanomaterials: Innovative therapeutic avenues for Alzheimer's disease management.

Alzheimer's Disease (AD) is a major global health challenge, largely due to its complex pathology and the limited effectiveness of existing treatments. Quercetin, a bioactive compound belonging to the flavonoid class, its promising antioxidant, anti-inflammatory, and neuroprotective effects in addressing AD. However, its therapeutic potential is hindered by challenges such as low bioavailability, instability, and restricted permeability across the blood-brain barrier (BBB).

Synergistic action of multiple degumming-related enzymes secreted by Bacillus subtilis XW-18: Decisive factor for driving the bio-degumming process of raw pineapple leaves.

Degumming, a process of removing gummy substances surrounding fiber, plays a crucial role in preparing plant fibers. This study clearly clarified that the multiple degumming enzymes by Bacillus subtilis XW-18 acted as a decisive factor for driving bio-degumming process of raw pineapple leaves. Firstly, PCR analysis verified that B.

Effects of Ultrasound-Assisted Treatment on Physicochemical Properties and Biological Activities of Polysaccharides from .

The aim of this study was to investigate the effect of ultrasonic treatment on the physicochemical properties and bioactivities of polysaccharides from samples (SPs) extracted with different solvents. The alkali-assisted extraction of polysaccharide (SPA), acid-assisted extraction of polysaccharides from (SPB), and hot water extraction of polysaccharides (SPCs) were perofrmed on . Ultrasonic treatment was performed with the SPA, SPB, and SPC in turn, and named USPA, USPB, and UPSC, respectively.

Modulation of gel characteristics in surimi-curdlan gel system by different valence metal cations: Mechanical properties, ionic distribution and microstructure visualization.

The valence state of metal cations is a potential factor in regulating the structural properties of surimi gels. In this study, Surimi-Curdlan dual network (SCDN) gel was prepared by NaCl, KCl, CaCl and MgCl, combined with the concept of dual network hydrogel. The effects of different valence metal cations on mechanical properties, water state, microstructure, protein structure and composition of SCDN gel were studied.

Amelioration of arsenic-induced hepatic injury via sulfated glycosaminoglycan from swim bladder: Modulation of Nrf2 pathway and amino acid metabolism.

Arsenic, a known environmental pollutant with a carcinogenic risk, is associated with chronic liver toxicity. Prebiotic regulation represents an emerging dietary strategy to alleviate arsenic-induced hepatotoxicity; however, research in this area remains limited. This study employed sulfated swim bladder glycosaminoglycan (SBSG), a potential prebiotic, to assess its efficacy in mitigating arsenic-induced liver injury.

A UHPLC-QE-MS-based metabolomics approach for the evaluation of fermented lipase by an engineered .

Using an engineered to produce lipase and can easily achieve high-level expression. The investigation of biochemical processes during lipase fermentation, approached from a metabolomics perspective, will yield novel insights into the efficient secretion of recombinant proteins. In this study, the lipase batch fermentation was carried out first with enzyme activity of 36.

The influence of pH-ultrasonic-induced myofibrillar protein conformation of Penaeus vannamei (Litopenaeus vannamei) on emulsification and digestion characteristics of fish oil oleogel-based emulsions.

pH-induced and ultrasound treatment can both adjust spatial conformation to improve the interfacial stability, and fish oil oleogel could be used to enhance oil binding capacity. The relationship between stabilization mechanism and lipid digestion was revealed, considering the protein conformation and interfacial characteristics. The results showed that pH-ultrasonic-induced myofibrillar proteins (MPs) at pH 7.