Differential expression of microRNA-3127-5p and its molecular mechanisms in polycystic ovary syndrome.

Introduction: The present study was undertaken to elucidate the expression status and molecular mechanism underlying microRNA-3127-5p (miR-3127-5p) in polycystic ovary syndrome (PCOS).

Material And Methods: A total of 50 PCOS and 50 non-PCOS patients were recruited as research subjects. Quantitative real-time polymerase chain reaction was employed to assess the relative abundances of miR-3127-5p in serum, cumulus cells (CCs), and granulosa cells (GCs) from PCOS patients.

Integrin factor (FAM27E3), as a metastatic marker of papillary thyroid carcinoma, through the p53 signaling pathway promoting lymph node metastasis.

Background: Papillary thyroid carcinoma (PTC) has a lymph node metastasis rate of 20%-90%. This study aims to explore the role and regulatory mechanism of the FAM27E3 in PTC lymph node metastasis.

Method: The seven thyroid cancer (THCA) datasets from the Gene Expression Omnibus (GEO) were combined as the training group, while the PTC dataset served as the testing group.

Association Between Digital Isolation and Sleep Disorders in Older Adults: Cross-Sectional and Longitudinal Study Using National Health and Aging Trends Study (NHATS) Data.

Background: As digital technology becomes increasingly embedded in daily life, digital isolation among older adults has become more pronounced. This isolation may restrict access to health information and social support, potentially leading to poorer sleep quality. However, most existing studies on digital isolation and sleep disorders were cross-sectional, lacking longitudinal evidence to establish causality.

Comparative Efficacy and Safety of Feng's Technique versus Radiofrequency Denervation for Cervical and Lumbar Facet Joint Pain: A Retrospective Study.

Background: Facet joint-mediated pain represents a major etiology of chronic spinal pain, posing persistent challenges in clinical management. We propose a novel technique-Feng's technique-which provides a novel and effective strategy for the treatment of facet joint-related pain.

Methods: This retrospective study analyzed 221 patients with cervical and lumbar facet joint pain treated between May 2020 and December 2021.

Non-invasive prediction of NSCLC immunotherapy efficacy and tumor microenvironment through unsupervised machine learning-driven CT Radiomic subtypes: a multi-cohort study.

Background: Radiomics analyzes quantitative features from medical images to reveal tumor heterogeneity, offering new insights for diagnosis, prognosis, and treatment prediction. This study explored radiomics based biomarkers to predict immunotherapy response and its association with the tumor microenvironment in non-small cell lung cancer (NSCLC) using unsupervised machine learning models derived from CT imaging.

Materials And Methods: This study included 1539 NSCLC patients from seven independent cohorts.

Ultrasound-guided peripheral nerve radiofrequency ablation for craniofacial postherpetic neuralgia: efficacy and safety in a retrospective cohort.

Background: Craniofacial postherpetic neuralgia (PHN), though relatively uncommon, presents significant clinical challenges through chronic headache and facial pain manifestations. This study aimed to assess the efficacy of ultrasound-guided peripheral nerve radiofrequency ablation (RFA) for craniofacial zoster-related pain.

Methods: A total of 91 patients with cephalofacial PHN admitted to the Renmin Hospital of Wuhan University from January 2023 and December 2024 were retrospectively analyzed.

Radiomics-Based Unsupervised Clustering Identifies Subtypes Associated With Prognosis and Immune Microenvironment in Clear Cell Renal Cell Carcinoma: A Multicenter Study.

Clear cell renal cell carcinoma (ccRCC) exhibits marked clinical heterogeneity, limiting the prognostic accuracy of traditional staging. We developed an unsupervised radiomics-based subtyping system integrating multi-omics data to decode tumor biology and improve risk stratification. Analyzing five cohorts (n = 1700, including surgical cohorts and an advanced ccRCC cohort receiving combined tyrosine kinase inhibitor and immunotherapy [T-I] treatment), we extracted 1834 CT radiomic features, applying consensus clustering to a discovery cohort (n = 748) and validating across centers.

Recompensation after transjugular intrahepatic portosystemic shunt reduces mortality risk: A long-term follow-up study.

Objective: This study aims to analyze the occurrence of recompensation after transjugular intrahepatic portosystemic shunt (TIPS) for patients with cirrhosis and portal hypertension and its impact on mortality risk.

Methods: The clinical data of 621 cirrhotic patients who underwent TIPS creation were retrospectively analyzed. Patients were categorized into a recompensation group (n = 126) and a non-recompensation group (n = 495).

Association between serum and red blood cell folate concentrations and urinary phthalate metabolite concentrations in US adults: evidence from a large population-based study.

Background: Studies have suggested that folate may mitigate the impact of exposure to environmental chemicals. We aimed to explore the relationship between blood folate biomarker concentrations and urine phthalate metabolites.

Methods: Based on data from the National Health and Nutrition Examination Survey spanning 2005 to 2016, 8,218 participants with measurements of folate biomarkers in blood and phthalates exposure in urine were included.

Cadmium induces autophagy via IRE1 signaling pathway activated by Ca in GC-2spd cells.

Cadmium (Cd), an environmental toxicant, accumulates in the human body and damages the male reproductive system. To investigate the molecular mechanisms underlying Cd-induced reproductive toxicity, we used GC-2spd cells and treated them with CdCl. Additionally, we added 2-APB (an inhibitor of the IP3R) and STF-083010 (an inhibitor of IRE1) to investigate whether they could ameliorate Cd-induced reproductive toxicity.

The predictive function of miR-122-5p and its action mechanism by regulating PKM2 in metabolic syndrome.

Background: Obesity will cause metabolic syndrome (Mets) easily, and its pathogenesis is not completely clear.

Aim: To probe into the predictive value of miR-122-5p and its regulatory mechanism in Mets.

Method: The predictive effect of miR-122-5p on Mets was evaluated by constructing a Receiver Operating Characteristic (ROC) curve.

Ultrasound combined with DSA-guided pulsed radiofrequency for perineal herpes zoster pain management: clinical outcomes and complications.

Background: Herpes zoster (HZ) in the perineal area is a rare disease. There are limited treatment options for this disease. This study aimed to assess the efficacy of ultrasound combined with digital subtraction angiography (DSA)-guided pulsed radiofrequency (PRF) for perineal herpes zoster-related pain.

Construction and Evaluation of a Predictive Model for Grassroots Nurses' Risk Perception of "Internet + Nursing Services": A Multicenter Cross-Sectional Study.

Purpose: The development of "Internet + nursing services" can effectively solve the problem of population aging, and grassroots nurses are the primary providers of such services in rural areas. This study aimed to analyze the factors affecting grassroots nurses' risk perception of "Internet + nursing services" and construct a predictive model.

Patients And Methods: A multicenter cross-sectional study of 2220 nurses from 27 secondary hospitals and 36 community health centers in Hubei Province was conducted from August to December 2023 using a multi-stage cluster sampling method.

Exploring the Impact of Adequate Energy Supply on Nutrition, Immunity, and Inflammation in Elderly Patients with Chronic Obstructive Pulmonary Disease.

Background: Chronic Obstructive Pulmonary Disease (COPD) progression in the elderly is notably influenced by nutritional, immune, and inflammatory status. This study aimed to investigate the impact of adequate energy supply on these indicators in COPD patients.

Methods: COPD patients meeting specific criteria were recruited and categorized into energy-adequate and energy-deficient groups based on their energy supply.

Effects of time-restricted eating on intrahepatic fat and metabolic health among patients with nonalcoholic fatty liver disease.

Objective: The study's objective was to explore whether early time-restricted eating (eTRE) and late time-restricted eating (lTRE) have different impacts on intrahepatic fat and metabolic health among patients with nonalcoholic fatty liver disease (NAFLD).

Methods: This is an 8-week, randomized, parallel-arm, open-label trial. Forty eligible patients were randomly assigned to eTRE (eating between 8:00 a.