Dual-functional DNA nanoribbon-templated copper nanoclusters synergistically activate NRF2/HO-1 pathway for synergistic oxidative stress mitigation.

Employing antioxidant nanozymes to eliminate reactive oxygen species (ROS) is a promising strategy for alleviating oxidative stress. However, most current nanozymes struggle to balance catalytic efficacy with biosafety, limiting their clinical applicability. In this study, we introduce a novel platform: DNA nanoribbon-templated copper nanoclusters (DNR/Cu NCs), which harness dual antioxidative mechanisms (direct ROS scavenging and activation of nuclear factor erythroid 2-related factor 2 (NRF2)/heme oxygenase-1 (HO-1) pathway) to synergistically mitigate oxidative stress.

Ultrasensitive Nanofluidic Detection of 17β-Estradiol in Natural Water by DNA Circuit-Mediated Hyperbranched DNA Nanowire Dual-Signal Amplification.

The pervasive detection of trace 17β-estradiol (E2) in aquatic ecosystems necessitates innovative analytical platforms capable of ultrahigh sensitivity and field applicability. Herein, we report a nanofluidic biosensor integrating polydopamine-functionalized graphene oxide (PDA/GO) membranes with an entropy-driven DNA circuit and hyperbranched DNA nanowires (HDW) for femtomolar-level E2 quantification. Leveraging E2-specific aptamer recognition, the system triggers an entropy-driven DNA circuit and subsequent hierarchical assembly of guanine quadruplex (G4)-enriched HDW nanostructures on nanochannel surfaces, amplifying interfacial electronegativity through phosphate backbone accumulation.

Cascade Catalytic Iron Phosphate Nanozyme-Driven Signal Amplification in S-Scheme AgBr/La-BiOBr-OV for Sensitive Dual-Channel Microfluidic PEC Detection of CA15-3 and CA125.

An innovative dual-channel microfluidic photoelectrochemical (PEC) immunosensor was constructed for simultaneous determination of carbohydrate antigen 15-3 (CA15-3) and cancer antigen 125 (CA125). Herein, AgBr-sensitized La-doped BiOBr with surface oxygen vacancies (AgBr/La-BiOBr-OV) was synthesized as a photoactive material to provide a stable photocurrent. Constructing an S-scheme heterojunction with AgBr and BiOBr facilitates the effective separation of photogenerated carriers.

A novel AuPt@CeVO bifunctional nanozyme with strong cascade SOD/POD-like catalytic activity enhances microfluidic photoelectrochemical signal amplification for ultrasensitive neuron-specific enolase detection.

A newly designed and highly effective cascade difunctional nanozyme, AuPt@CeVO, has been developed to function as a microfluidic photoelectrochemical (PEC) signal label, enabling the highly sensitive identification of bioproteins. This study utilized Z-scheme CuI/BiOI photoactive nanocomposites by applying CuI onto the BiOI nanoarray's surface, which serves as a sensor matrix to obtain an improved and stable photocurrent. The nanoarray arrangement in BiOI greatly improves the consistency and durability of the sensing platform.

Detection of Matrix Metalloproteinase 2 by a Polypeptide Cleavable Split-Type Sensor Based on Atomically Precise Bimetallic Nanocluster AuAg(MSA).

In this study, a split-type electrochemiluminescence (ECL) sensor based on polypeptide cleavage was proposed for the sensitive detection of matrix metalloproteinase 2 (MMP 2). The bimetallic nanoclusters were protected by mercaptosuccinic acid (MSA), named AuAg(MSA), serving as a signal probe. Owing to the synergistic effect of the bimetal composition, the clusters exhibited enhanced ECL properties.

Highly Electroactive ZIF-67-Derived NiCo-LDH Nanocages as an Efficient Coreaction Accelerator for Amplifying the Electrochemiluminescence of Graphdiyne-Based Quantum Dot-Functionalized ZIF-8 in the Biomarker Immunoassay.

The exploitation of innovative electrochemiluminescence (ECL) luminophores with anticipated performance and the design of an effective sensing strategy have become breakthrough research in the fields of ECL immunoassays. Herein, graphdiyne-based quantum dot (GDYQD)-modified zeolitic imidazolate framework-8 (GDYQDs/ZIF-8) was utilized as an ECL luminophore, whereas zeolitic imidazolate framework-67 (ZIF-67)-derived NiCo-layered double hydroxide (NiCo-LDH) modified by AuPd nanoparticles (NPs) (NiCo-LDH@AuPd) was employed as a coreaction accelerator for the construction of an immunosensing strategy. The GDYQDs, as a derivative of graphdiyne, possessed excellent biological properties owing to their active units and abundant surface defects, attracting considerable attention in the ECL field.

Dual-quenching sensing system from Cu-MOFs/SOx to near-infrared electrochemiluminescence silver nanosheets for sensitive analysis of CEA.

In this study, a highly sensitive biosensor for the detection of carcinoembryonic antigen (CEA) was developed by integrating the excellent properties of a near-infrared electrochemiluminescence (NIR ECL) substance and a double ECL signal quencher. Silver nanosheets (Ag-Cys) exhibiting NIR electrochemiluminescence were synthesized via a simple chemical reaction using AgNO and l-cysteine (L-Cys) as precursors. The incorporation of L-Cys not only reduces photochemical damage but also preserved the antigen-binding activity, facilitating the construction of a high-performance immunosensor.

Electrochemiluminescence sensor based on Ag/ZnO nanomaterial-enhanced GPTMS/FeCdS@FeInS for sensitive analysis of CD44.

Incorporating an additional metal ion into binary metal sulfides to adjust the band gap and carrier mobility, thereby forming ternary metal sulfides, has significant potential in the field of electrochemical luminescence. This research represents the first application of FeCdS@FeInS as an innovative electrochemiluminescence (ECL) emitter. To further enhance the ECL signal, ZIF-8-derived Ag-doped ZnO nanocomposites were engineered as co-reaction accelerators, leveraging their exceptional catalytic activity to enhance the FeCdS@FeInS/KSO system through efficient generation of SO˙ radicals.

Development of Zn-Based Tower-like Photoelectrochemical Sensor Array for Sandwich-Type Immunosensing Analysis of Tau.

A novel tower-like photoelectrochemical (PEC) sensor array, incorporating a self-calibration strategy, has been developed. In this study, Zn foil was folded into a quadrangular prism, upon which ZnO nanorods were grown in situ via a hydrothermal process. Subsequently, these ZnO nanorods were sensitized with narrow bandgap CdSe nanospheres.

Interface self-shelling effect-mediated photoinduced carrier transport and multiplexed signal amplification mechanism in self-powered photoelectrochemical biosensing.

In the realm of biomedical diagnostics, the development of sensitive and specific detection methods for cancer biomarkers is of paramount importance. Herein, we report on the design and implementation of a self-powered photoelectrochemical (PEC) sensor that harnesses amplified photocathode signals for the deterioration of carbohydrate antigen 125 (CA125) associated with ovarian cancer. This self-powered sensing platform integrates CuO/CuSnS heterojunction and ZnInS sensitized TiO with flower-like structure as photocathode and photoanode.

Smartphone-Controlled Portable Photoelectrochemical Biosensor Exploiting Chirality-Resolved Ratiometric Sensing Strategy: A Proof of Concept.

Integrating ratiometric photoelectrochemical (PEC) strategy with a smartphone-based portable biosensor to build a bioanalysis device is usually subject to large-volume space-resolved facilities and is picky about the polarity or excitation wavelength of the photoactive materials. Herein, a chirality-resolved ratiometric portable PEC biosensor on a platform rich in crystalline-amorphous interfaces addresses this issue. Concretely, the crystalline BiWO bulk phase/amorphous BiOBr surfaces with BiS nanoparticles attached (a-BWO-s) platform and the paired photoactive markers with specific chirality FeO@Ag-l-(and d-)histidine are integrated on the portable biosensor.

Mesostructured Silica Xerogel-Encapsulated Gold Nanoclusters as an Electrochemiluminescence Emitter Combined with a DNA Walker Amplification Strategy for Detection of Ochratoxin A.

Gold nanoclusters (AuNCs) exhibit unique optical properties and satisfactory biocompatibility, enabling them as highly promising electrochemiluminescence (ECL) emitters. However, the low ECL efficiency, resulting from the free movement of ligands, significantly hinders the development of AuNCs in the sensing field. In this work, polyelectrolytes were utilized to encapsulate AuNCs within the mesostructured silica xerogel, forming mesostructured silica xerogel-encapsulated gold nanoclusters (AuNCs@MSX).

A self-powered PFC sensing platform via integrating aptamer-modified photoanode and Z-scheme signal amplifying photocathode for microcystin-LR detection.

Background: Microcystin-LR (MC-LR) is a hepatotoxin produced by cyanobacteria, which is biologically toxic, prevalent, and has serious effects on human health. Therefore, it is urgent to develop miniaturized and cost-efficient methods for MC-LR monitoring. Photocatalytic fuel cell (PFC) sensing has already been tipped as a promising approach for facile, on-site detection in environmental mediators.

Inverse Oxide/Alloy-Structured Nanozymes with NIR-Triggered Enzymatic Cascade Regulation of ROS Homeostasis for Efficient Wound Healing.

The precise spatiotemporal control of reactive oxygen species (ROS) generation and scavenging remains pivotal for infected wound healing. However, conventional nanozymes fail to adaptively regulate ROS dynamics across inflammatory and proliferative phases. A near-infrared (NIR)-activated inverse oxide/alloy-structured nanozyme (CoFe/ZnO@C) is developed, featuring enzymatic cascade activities to tune ROS homeostasis through synergistic chemodynamic (CDT), photodynamic (PDT), and photothermal (PTT) therapies.

Multivalent Redox Reversible Conversion-Enhanced Electrochemiluminescence Strategy for Progesterone Detection.

The performance of electrochemiluminescence (ECL) sensing platforms, especially the sensitivity, relies on an efficient signal enhancement mechanism and presents a critical challenge in developing novel strategies simultaneously. In this work, we reported a multivalent redox reversible conversion-enhanced ECL strategy based on the redox reactions of two pairs of multivalent metal elements on the electrode surface. TiO-loaded Eu(OH) (Eu(OH)@TiO) was introduced as a dual-enhanced coreactant accelerator to catalyze the oxidation process of tripropylamine (TPrA), thus improving the reaction rate at the sensing interface and the ECL strength of the luminophore.

Advanced Dual-Mode Microfluidic Sensing Platform Based on Amphiphilic Polymer-Capped Perovskite Nanozymes Induced Photoelectrochemical Signal Amplification and Fluorescence Emission.

A novel dual-mode microfluidic sensing platform integrating photoelectrochemical (PEC) and fluorescence (FL) sensors was developed for the sensitive monitoring of heart fatty acid binding protein (h-FABP). First, BiVO/AgInS (BVAIS) composites with excellent photoelectric activity were synthesized as sensing matrices. The BVAIS heterojunction with a well-matched internal energy level structure provided a stable photocurrent.

Aggregation-Induced Electrochemiluminescence of Silica-Confined Tetraphenylethylene with Pd Nanocube-Loaded CoO Nanosheets as a Coreaction Accelerator for Sensitive Bioanalysis.

Aggregation-induced electrochemiluminescence (AIECL) provides a new approach for the development of novel electrochemiluminescence (ECL) strategies. Herein, a biosensor was constructed by incorporating 1,1,2,2-tetra(4-carboxylphenyl)ethylene (HTCPE) into a mesoporous silica nanosphere (MSN) to obtain a highly organized AIECL luminophore of (MSN-HTCPE) for signal antibody (Ab) labeling and using Pd nanocube (NC)-loaded CoO nanosheets (NSs) (PdNCs/CoONSs) as a novel coreaction accelerator. The confinement of HTCPE molecules in the MSN restricted the intramolecular rotation and thus enhanced the radiation transition of HTCPE.

Hexagonal Prism-Shaped AIE-Active MOFs as Coreactant-Free Electrochemiluminescence Luminophores Coupled with Hollow CuO@Pd Heterostructures as Efficient Quenching Probes for Sensitive Biosensing.

For most self-luminous metal-organic framework (MOF)-involved electrochemiluminescence (ECL) systems, the integration of exogenous coreactants is indispensable to promote ECL efficiency. However, the introduction of a coreactant into an electrolyte would result in poor stability, thereby inevitably affecting analytical accuracy. Herein, by employing aggregation-induced emission luminogens as ligands, we first synthesized one hexagonal prism-shaped MOF that displays robust and steady ECL signal without an exogenous coreactant.

Dye-sensitized NiO photocathode sensor based on signal-sensitive change strategy for MC-LR detection.

A novel photoelectrochemical (PEC) sensor for the detection of microcystic toxins (MC-LR) was developed on the basis of signal-sensitive change strategy. NiO nanoarray as a basic photoactive material was grown directly on the ITO glass electrode via calcination after hydrothermal reaction, while dye N719 was used to sensitize the electrode for enhancing visible light absorption, and the first signal-on stage was obtained. In the meantime, p-type CuO was applied as the signal probe attached to probe DNA (DNA) to improve the sensitivity, and the second "signal-on" stage appeared because of its synergistic effect with NiO nanoarrays.

Analysis of okadaic acid using electrochemiluminescence imaging on microfluidic biosensing chip.

The sensitivity and specificity of electrochemiluminescence (ECL)-based biosensor directly rely on the property of luminophor, the type of sensing carriers and the effectiveness of signal amplification used in the sensor design, which poses a major challenge to manage these elements simultaneously. In this work, an aggregation-induced electrochemiluminescence (AIECL) microfluidic sensing chip using 4',4″,4‴,4‴'-(ethene-1,1,2,2-tetrayl)tetrabiphenyl-4-carboxylic acid (TPE)-derived hafnium-based metal-organic framework (Hf-MOF) as emitter was developed. An easily overlooked marine pollutant, okadaic acid (OA) with different concentrations ranging from 5.

Self-Assembled Perylene Diimide (PDI) Nanowire Sensitized InO@MgInS S-Scheme Heterojunction as Photoelectrochemical Biosensing Platform for the Detection of CA15-3.

Inorganic/organic heterojunctions show promising applications as high-performance sensing platforms for photoelectrochemical (PEC) immunosensors. This work reports constructing a PEC biosensor for CA15-3 based on a self-assembled perylene diimide (PDI) nanowire sensitized InO@MgInS S-scheme heterojunction platform. P-type semiconductor CuO nanoparticles were designed as a signal burst source and were used as immunoassay labels.

1,1,2,2-Tetra(4-carboxylphenyl)ethylene-Based Metal-Organic Gel as Aggregation-Induced Electrochemiluminescence Emitter for the Detection of Aflatoxin B1 Based on Nanosurface Energy Transfer.

In this Letter, a sensitive DNA sensing platform was developed using an indium-ion-coordinated 1,1,2,2-tetra(4-carboxylphenyl)ethylene (TPE) metal-organic gel (In-MOG) as an aggregation-induced electrochemiluminescence (AIECL) emitter and nanosurface energy transfer (NSET) as an efficient quenching strategy for detecting aflatoxin B1 (AFB1), the most dangerous food toxin. The coordination occurred in indium ions, and carboxyl groups restricted the internal rotation and vibration of TPE molecules, forcing them to release photons via radiative transitions. The quenchers of microfluidic-produced gold nanoparticles were embedded in a long-tailed triangular DNA structure, where the quenching phenomenon aligned with the theory of ECL-NSET under the overlap of spectra and appropriate donor-acceptor spacing.