Trophoblast cell response to periodontal pathogens unveils oxidative stress networks between periodontitis and pre-eclampsia: Mendelian randomization, transcriptomic analysis, and cellular experimental validation.

Background: Periodontitis has been associated with an increased risk of pre-eclampsia, but the underlying mechanisms and causal relationship remain unclear. Oxidative stress has been implicated in both conditions, suggesting a potential mechanistic link.

Objectives: To investigate the causal relationship between periodontitis and pre-eclampsia and elucidate shared molecular pathways, with a focus on oxidative stress-related mechanisms.

Study on the regulation of gastric cancer cell apoptosis by LACTB through mitochondrial autophagy pathway.

This study aimed to determine whether β-lactamase-like protein (Lactamase-β, LACTB) influences apoptosis in gastric cancer cells by modulating mitochondrial autophagy through the PTEN-induced putative kinase 1 (PINK1) or Parkin pathway. Firstly, the expression level of LACTB in gastric cancer tissues was detected by immunohistochemistry, and the survival data of patients were used to explore the relationship between LACTB expression level and patient prognosis. Secondly, LACTB overexpression (+ LACTB) and knockdown (sh-LACTB) AGS gastric cancer cell lines were constructed; flow cytometry and other experiments were used to detect the effect of LACTB on AGS cell apoptosis; Western Blot was used to detect the expression of PINK1/Parkin mitochondrial autophagy pathway-related proteins and lysosome-related proteins in + LACTB and sh-LACTB gastric cancer cells; kits and electron microscopy were used to detect changes in the number of reactive oxygen species (ROS) and autophagosomes.

Dual-quenching sensing system from Cu-MOFs/SOx to near-infrared electrochemiluminescence silver nanosheets for sensitive analysis of CEA.

In this study, a highly sensitive biosensor for the detection of carcinoembryonic antigen (CEA) was developed by integrating the excellent properties of a near-infrared electrochemiluminescence (NIR ECL) substance and a double ECL signal quencher. Silver nanosheets (Ag-Cys) exhibiting NIR electrochemiluminescence were synthesized via a simple chemical reaction using AgNO and l-cysteine (L-Cys) as precursors. The incorporation of L-Cys not only reduces photochemical damage but also preserved the antigen-binding activity, facilitating the construction of a high-performance immunosensor.

Electrochemiluminescence sensor based on Ag/ZnO nanomaterial-enhanced GPTMS/FeCdS@FeInS for sensitive analysis of CD44.

Incorporating an additional metal ion into binary metal sulfides to adjust the band gap and carrier mobility, thereby forming ternary metal sulfides, has significant potential in the field of electrochemical luminescence. This research represents the first application of FeCdS@FeInS as an innovative electrochemiluminescence (ECL) emitter. To further enhance the ECL signal, ZIF-8-derived Ag-doped ZnO nanocomposites were engineered as co-reaction accelerators, leveraging their exceptional catalytic activity to enhance the FeCdS@FeInS/KSO system through efficient generation of SO˙ radicals.

Self-Generative Singlet Oxygen (O)-Initiated Chemical Modification of Nuclear DNAs Combats Tumor Drug Resistance.

Multidrug resistance (MDR) is one of the major problems in cancer treatment. Overcoming MDR to achieve effective cancer treatment remains a huge challenge. Here, we proposed a self-generative singlet oxygen (O)-initiated chemical modification of nuclear DNAs (SiCMoND) approach to kill multidrug-resistant tumor synergizing with chemotherapy.

Survival Outcomes and Prognostic Factors in Follicular Lymphoma-Grade 3: A Study Based on the SEER Database.

ObjectivesFollicular lymphoma-grade 3 is an aggressive subtype of Follicular lymphoma with a higher recurrence risk and poorer survival outcomes compared to lower-grade Follicular lymphoma. Existing prognostic models often lack accuracy due to disease heterogeneity and insufficient integration of demographic and treatment variables. This study aimed to identify independent prognostic factors and develop a nomogram for predicting OS in Follicular lymphoma-grade 3 patients using the SEER database.

Molecular screening to track ceftriaxone-resistant FC428-like strains' dissemination in four provinces of China, 2019 to 2021.

BackgroundThe global dissemination of ceftriaxone-resistant FC428-like strains poses a public health concern. To assess and follow their spread, establishing effective antimicrobial resistance (AMR) surveillance systems is essential.AimThis study aimed to track ceftriaxone-resistant FC428-like strains in parts of China, using a molecular screening tool.

Identification of the regulatory role of SsMYBS25-4 in salt stress from MYB-related transcription factors in sugarcane (Saccharum spontaneum).

Sugarcane is a highly valued crop known for its significant production of sugar and biomass. MYB transcription factors (TFs) are critical regulators in plant growth and stress tolerance, but MYB-related genes, an atypical subset of the MYB family, remain less explored. In this study, we identified 119 MYB-related genes in the genome of wild sugarcane (S.

A Phase I/IIa Clinical Trial to Evaluate Safety and Adrenal Uptake of Para-Chloro-2-[F]Fluoroethyletomidate in Healthy Volunteers and Patients with Primary Aldosteronism.

Primary aldosteronism (PA) is a common, potentially reversible, cause of hypertension. Distinguishing unilateral from bilateral PA is critical when deciding who should be offered surgery (unilateral adrenalectomy). Recent studies have shown that PET/CT with [C]metomidate can accurately identify unilateral PA, with localization of the causative aldosterone-producing adenoma (APA).

Cross-sectional study of proteomic differences between moderate and severe psoriasis.

Although an ongoing understanding of psoriasis vulgaris (PV) pathogenesis, little is known about the proteomic differences between moderate and severe psoriasis. In this cross-sectional study, we evaluated the proteomic differences between moderate and severe psoriasis using data-independent acquisition mass spectrometry (DIA-MS). 173 differentially expressed proteins (DEPs) were significantly differentially expressed between the two groups.

C1 Posterior Arch Screws for the Additional Reinforcement of Upper Cervical Spine Fixation: Surgical Technique and Preliminary Case Series.

Objective: This study aims to evaluate the clinical outcomes of using C1 posterior arch screws (PASs) combined with C2 translaminar screws as an adjunct for reinforcing upper cervical spine fixation.

Methods: A retrospective analysis was conducted on 4 male patients who underwent surgery involving C1 PASs and C2 translaminar screws between January 2022 and February 2024. Surgical technique involved the insertion of standard C1 lateral mass screws and C2 pedicle screws, followed by the placement of C1 PASs and C2 translaminar screws for additional fixation.

Research Progress on the Posterior Midline Lumbar Spinous Process-Splitting Approach.

The traditional posterior median approach laminectomy is widely used for lumbar decompression. However, the bilateral dissection of paraspinal muscles during this procedure often leads to postoperative muscle atrophy, chronic low back pain, and other complications. The posterior midline spinous process-splitting approach (SPSA) offers a significant advantage over the traditional approach by minimizing damage to the paraspinal muscles.

The performance of a novel diagnostic criteria for neurosyphilis in HIV-negative patients.

Diagnosing neurosyphilis in clinical settings poses significant challenges due to the absence of highly efficient diagnostic criteria. Our objective was to enhance the existing diagnostic criteria and assess their sensitivity and specificity for identifying neurosyphilis in HIV-negative patients. We conducted a retrospective review of patient records from a cross-sectional study carried out between December 2019 and May 2023.

Brain tissue oxygen pressure combined with intracranial pressure monitoring may improve clinical outcomes for patients with severe traumatic brain injury: a systemic review and meta-analysis.

Background: Although the optimization of brain oxygenation is thought to improve the prognosis, the effect of brain tissue oxygen pressure (PbtO2) for patients with severe traumatic brain injury (STBI) remains controversial. Therefore, the present study aimed to determine whether adding PbtO2 to intracranial pressure (ICP) monitoring improves clinical outcomes for patients with STBI.

Methods: PubMed, Embase, Scopus and Cochrane Library were searched for eligible trials from their respective inception through April 10th, 2024.

Role of gut microbiota and metabolomics in the lipid-lowering efficacy of statins among Chinese patients with coronary heart disease and hypercholesterolemia.

Background: Statins, being the primary pharmacological intervention for hypercholesterolemia, exhibit a notable degree of interpatient variability in their effectiveness, which may be associated with gut microbiota. This study sought to identify the biomarkers for evaluating differences in statin efficacy.

Methods: A quasi case-control study was conducted among participants with hypercholesterolemia and coronary heart disease taking rosuvastatin essential.

Association Between Serine Concentration and Coronary Heart Disease: A Case-Control Study.

Purpose: Early identification of new residual risk factors for coronary heart disease (CHD) is warranted. In this study, we aim to investigate the association between the serine concentration, an important amino acid in one-carbon metabolism, and CHD in Chinese hospitalized patients.

Patients And Methods: This case-control study included 428 case-control pairs comprising patients with CHD with a maximum coronary artery stenosis degree of >70% and controls with stenosis of <30%.

The application of nanomaterials in tumor therapy based on the regulation of mechanical properties.

Mechanical properties, as crucial physical properties, have a significant impact on the occurrence, development, and metastasis of tumors. Regulating the mechanical properties of tumors to enhance their sensitivity to radiotherapy and chemotherapy has become an important strategy in the field of cancer treatment. Over the past few decades, nanomaterials have made remarkable progress in cancer therapy, either based on their intrinsic properties or as drug delivery carriers.

Analysis of molecular epidemiological characteristics and antimicrobial susceptibility of vancomycin-resistant and linezolid-resistant Enterococcus in China.

Background: This study investigates the distribution and characteristics of linezolid and vancomycin susceptibilities among Enterococcus faecalis (E. faecalis) and Enterococcus faecium (E. faecium) and explores the underlying resistance mechanisms.

Weakly Supervised Pose Estimation of Surgical Instrument from a Single Endoscopic Image.

Instrument pose estimation is a key demand in computer-aided surgery, and its main challenges lie in two aspects: Firstly, the difficulty of obtaining stable corresponding image feature points due to the instruments' high refraction and complicated background, and secondly, the lack of labeled pose data. This study aims to tackle the pose estimation problem of surgical instruments in the current endoscope system using a single endoscopic image. More specifically, a weakly supervised method based on the instrument's image segmentation contour is proposed, with the effective assistance of synthesized endoscopic images.

Insertion sequence transposition activates antimycobacteriophage immunity through an -silenced lipid metabolism gene island.

Insertion sequences (ISs) exist widely in bacterial genomes, but their roles in the evolution of bacterial antiphage defense remain to be clarified. Here, we report that, under the pressure of phage infection, the IS transposition of into the gene can occur at high frequencies, which endows the mutant mycobacterium with a broad-spectrum antiphage ability. Lsr2 functions as a negative regulator and directly silences expression of a gene island composed of 11 lipid metabolism-related genes.

Electrochemiluminescence quenching effect of CuO towards flower-like ferric ion-doped g-CN and its application for Cyfra21-1 immunosensing.

In this article, ferric ion-doped floral graphite carbon nitride (Fe-CN-3, energy donor) was used to construct the substrate of the immunosensor and copper oxide nanocubes (CuO, energy acceptor) were taken as an efficient ECL quenching probe. A sandwich quench electrochemiluminescence (ECL) immunosensor for soluble cytokeratin 19 fragment (Cyfra21-1) detection was preliminarily developed based on a novel resonant energy transfer donor-acceptor pair. Fe-CN-3, a carbon nitride that combines the advantages of metal ion doping as well as morphology modulation, is used in ECL luminophores to provide more excellent ECL performance, which makes a significant contribution to the application and development of carbon nitride in the field of ECL biosensors.