Evaluating the role of Kasai portoenterostomy in biliary atresia older than 90 days.

Purpose: Biliary atresia (BA) patients presenting after 90 days of age face contentious treatment decisions between Kasai portoenterostomy and direct liver transplantation. This study evaluated outcomes of Kasai portoenterostomy in older BA patients to inform therapeutic decision-making.

Methods: A retrospective multicenter study analyzed 32 BA patients who underwent Kasai portoenterostomy beyond 90 days of age.

Multi-omics integrated analysis to generate transcriptome and protome profiles of resveratrol improving Paraquat-induced acute lung injury.

Paraquat (PQ), a widely used non-selective herbicide, poses significant health risks, with as little as 30 mL potentially causing fatal poisoning due to acute lung injury (ALI). Despite frequent cases, effective treatments for PQ poisoning are limited, highlighting the need for in-depth investigation into the mechanisms underlying PQ-induced ALI. Resveratrol was identified as a therapeutic agent based on the key differentially regulated pathways.

Association between gut microbiota and allergic rhinitis: a systematic review and meta-analysis.

Background: Many studies have shown that allergic rhinitis (AR) is closely related to intestinal flora, and probiotics are effective in treatment. However, the results of human observational studies on the correlation between intestinal flora and AR have been contradictory. The aim of this study was to determine the relationship between gut microbiota and allergic rhinitis and to provide a clinical reference.

Identification of novel metabolism-related biomarkers of Kawasaki disease by integrating single-cell RNA sequencing analysis and machine learning algorithms.

Background: The bile acid metabolism (BAM) and fatty acid metabolism (FAM) have been implicated in Kawasaki disease (KD), but their precise mechanisms remain unclear. Identifying signature cells and genes related to BAM and FAM could offer a deeper understanding of their role in the pathogenesis of KD.

Method: We analyzed the public single-cell RNA sequencing (scRNA-seq) dataset GSE1687323 to characterize the immune cell-type landscape in KD.

Genomic analysis and therapeutic efficacy evaluation of bacteriophage PK2420 for pneumonia caused by hypervirulent (K20 serotype).

Hypervirulent (hvKp) strains are increasingly recognized for their aggressive nature, which leads to severe clinical outcomes. The emergence of multidrug-resistant strains constitutes a substantial challenge for clinical management. Phage therapy offers a potential solution to the antibiotic resistance crisis.

Parallel comparison of T cell and B cell subpopulations of adenoid hypertrophy and tonsil hypertrophy of children.

The adenoids and tonsils are important immune organs of the nasopharynx that often become hypertrophic in childhood because of recurrent pathogen infection. However, the differences in the immune microenvironment of adenoid hypertrophy (AH) and tonsil hypertrophy (TH) are unclear. Here, we show the epidemiological characteristics and peripheral blood cell indices of 1209 pediatric patients (1-15 years old) diagnosed with AH, and find that AH is often accompanied by TH and characterized by specific changes in immune cell types.

Risk factors for non-response to initial IVIG plus methylprednisolone therapy in children with Kawasaki disease.

Objectives: IVIG plus corticosteroids is recommended as the initial intensified therapy for high-risk IVIG-resistant Kawasaki disease (KD) patients. However, some patients still require additional rescue therapy despite this treatment. This study aimed to identify risk factors associated with non-response to initial IVIG plus methylprednisolone therapy in high-risk refractory KD patients.

Intrahepatic ductoplasty effectively corrects the ductal dilatation in Todani type IV-A choledochal cyst in children.

Primary ductal stricture and intrahepatic duct dilatation are characteristic features in Todani type IV-A choledochal cysts (CDC) and necessitate thorough evaluation and management during surgical treatment. This study aimed to present our experience with ductoplasties for type IV-A CDCs with primary ductal strictures. Between June 2015 and June 2022, 54 patients were reviewed.

Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissue.

Introduction: This study explores the effects of moderate-intensity exercise on protein lactylation in mouse muscle tissue metabolism.

Methods: Healthy adult mice running for 6 weeks as an exercise model and sedentary mice as the control were used to perform transcriptomic, proteomic, lactylation-proteomic, and metabolomic analyses. Correlation analysis between transcriptome and proteome and between proteome and metabolome was also conducted.

Transcriptome analysis of liver injury of fatty liver disease induced by ALDH2 deficiency.

Aldehyde dehydrogenase 2 (Aldh2) Glu504Lys mutation, common in East Asians, is linked to various alcohol-related pathologies, notably fatty liver disease. Recent findings suggest that high ethanol-producing Klebsiella pneumoniae(HiAlc Kpn) exacerbates liver injury in non-alcoholic fatty liver disease (NAFLD). Our study investigated the combined effects of Aldh2 deficiency and HiAlc Kpn on NAFLD liver injury, transcriptome analyses to unearth potential mechanisms and therapeutic targets.

Effects of moderate intensity exercise on liver metabolism in mice based on multi-omics analysis.

Physical exercise is beneficial to keep physical and mental health. The molecular mechanisms underlying exercise are still worth exploring. The healthy adult mice after six weeks of moderate-intensity exercise (experimental group) and sedentary mice (control group) were used to perform transcriptomic, proteomic, lactylation modification, and metabolomics analysis.

Single-cell RNA sequencing uncovers molecular mechanisms of intravenous immunoglobulin plus methylprednisolone in Kawasaki disease: attenuated monocyte-driven inflammation and improved NK cell cytotoxicity.

Introduction: Intravenous immunoglobulin (IVIG) plus methylprednisolone as initial intensive therapy or additional therapy in Kawasaki disease (KD) has been used in clinical practice. However, its molecular and cellular mechanism is unclear.

Methods: We performed single-cell analysis on 14 peripheral blood mononuclear cell (PBMC) samples obtained from 7 KD patients who received either IVIG monotherapy or IVIG plus methylprednisolone therapy.

Progression prediction of coronary artery lesions by echocardiography-based ultrasomics analysis in Kawasaki disease.

Background: Echocardiography-based ultrasomics analysis aids Kawasaki disease (KD) diagnosis but its role in predicting coronary artery lesions (CALs) progression remains unknown. We aimed to develop and validate a predictive model combining echocardiogram-based ultrasomics with clinical parameters for CALs progression in KD.

Methods: Total 371 KD patients with CALs at baseline were enrolled from a retrospective cohort (cohort 1, n = 316) and a prospective cohort (cohort 2, n = 55).

Single-Incision Retroperitoneal Laparoscopic Resection of Adrenal Tumors in Children.

Background: We describe our experience with single-incision retroperitoneal laparoscopic (SIRL) for resection of adrenal tumors in pediatric patients and discuss the technique's clinical value.

Methods: We retrospectively analyzed clinical data of 27 pediatric patients who underwent SIRL between January 2020 and September 2023. Patients with tumors >5 cm in size and those requiring vascular skeletonization surgery or extensive lymph node dissection were excluded.

Relationships of serum MMP-7 and clinical characteristics in choledochal cyst children.

Background: Matrix metalloproteinase-7 (MMP-7) is associated with biliary injury. This study aimed to evaluate the relationships of serum MMP-7 with clinical characteristics in choledochal cysts (CDC) children.

Methods: Between June 2020 and July 2022, we conducted a prospective study of CDCs who underwent one-stage definitive operation at our center.

Development of a Recombinase-Aided Amplification Assay for the Rapid Detection of .

() was first discovered in Japan in 2009 and has since spread worldwide. It exhibits strong transmission ability, high multidrug resistance, blood infectivity, and mortality rates. Traditional diagnostic techniques for have shortcomings, leading to difficulty in its timely diagnosis and identification.

Multiplexing of TMT labeling reveals folate-deficient diet-specific proteome changes in NTDs.

In the early stage of embryonic development, the neural tube (NT) cannot be closed properly due to some complex factors, including environmental factors, genetic factors, and the relationship between various factors, leading to the occurrence of neural tube defects (NTDs). In this study, we induced a mouse model of NTDs by feeding mice with a low-folate diet and intraperitoneally injecting them with 1.5 mg/kg methotrexate on E7.

Acetylome analyses provide novel insights into the effects of chronic intermittent hypoxia on hippocampus-dependent cognitive impairment.

Introduction: Chronic intermittent hypoxia (CIH) can negatively affect hippocampal function through various molecular mechanisms. Protein acetylation, a frequently occurring modification, plays crucial roles in synaptic plasticity and cognitive processes. However, the global protein acetylation induced by CIH in the hippocampus and its specific effects on hippocampal function and behavior remain poorly understood.

Single-Cell Transcriptome Reveals Potential Mechanisms for Coronary Artery Lesions in Kawasaki Disease.

Background: Coronary artery lesions (CALs) are the most common and major complication of Kawasaki disease (KD) in developed countries. However, the underlying immunologic mechanisms of CAL development in KD remain unclear.

Methods: Here, we conducted single-cell transcriptome analyses of 212 210 peripheral blood mononuclear cells collected from a cross-sectional cohort of 16 children, including 4 patients with KD with CALs, 5 patients with KD without CALs, 4 healthy controls, and 3 febrile controls.

Risk factors and an early predictive model for Kawasaki disease shock syndrome in Chinese children.

Background: Kawasaki disease shock syndrome (KDSS), though rare, has increased risk for cardiovascular complications. Early diagnosis is crucial to improve the prognosis of KDSS patients. Our study aimed to identify risk factors and construct a predictive model for KDSS.

Development of a prediction model for progression of coronary artery lesions in Kawasaki disease.

Backgrounds: This study aimed to identify risk factors for the progression of coronary artery lesions (CALs) in children with Kawasaki disease (KD) and to develop a nomogram prediction model.

Methods: This is a retrospective case-control study in which the participants were categorized into three groups based on the changes of the maximum Z score (Zmax) of coronary arteries at the 1-month follow-up compared with the baseline Zmax: CALs-progressed, CALs-improved, and CALs-unchanged.

Results: Of total 387 patients, 65 (27%), 319 (73%), and 3 (0.