Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Purpose: Periodontitis-associated bacteria, such as Porphyromonas gingivalis and Fusobacterium nucleatum, are closely linked to the risk of oral squamous cell carcinoma (OSCC). Emerging studies have indicated that another common periodontal pathogen, Prevotella intermedia (P. intermedia), is enriched in OSCC and could affect the occurrence and progression of OSCC. Our aim is to determine the effects of P. intermedia on the progression of OSCC and the role of antibiotics in reversing these effects.
Methods: In this study, a murine xenograft model of OSCC was established, and the mice were injected intratumorally with PBS (control group), P. intermedia (P.i group), or P. intermedia combined with an antibiotic cocktail administration (P.i + ABX group), respectively. The effects of P. intermedia and ABX administration on xenograft tumor growth, invasion, angiogenesis, and metastasis were investigated by tumor volume measurement and histopathological examination. Enzyme-linked immunosorbent assay (ELISA) was used to investigate the changes in serum cytokine levels. Immunohistochemistry (IHC) was adopted to analyze the alterations in the levels of inflammatory cytokines and infiltrated immune cells in OSCC tissues of xenograft tumors. Transcriptome sequencing and analysis were conducted to determine differential expression genes among various groups.
Results: Compared with the control treatment, P. intermedia treatment significantly promoted tumor growth, invasion, angiogenesis, and metastasis, markedly affected the levels of inflammatory cytokines, and markedly altered M2 macrophages and regulatory T cells (Tregs) infiltration in the tumor microenvironment. However, ABX administration clearly abolished these effects of P. intermedia. Transcriptome and immunohistochemical analyses revealed that P. intermedia infection increased the expression of interferon-stimulated gene 15 (ISG15). Correlation analysis indicated that the expression level of ISG15 was positively correlated with the Ki67 expression level, microvessel density, serum concentrations and tissue expression levels of inflammatory cytokines, and quantities of infiltrated M2 macrophages and Tregs. However, it is negatively correlated with the quantities of infiltrated CD4 and CD8 T cells.
Conclusion: In conclusion, intratumoral P. intermedia infection aggravated OSCC progression, which may be achieved through upregulation of ISG15. This study sheds new light on the possible pathogenic mechanism of intratumoral P. intermedia in OSCC progression, which could be a prospective target for OSCC prevention and treatment.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11033248 | PMC |
| http://dx.doi.org/10.1007/s00432-024-05730-5 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Prussian Blue Nanoparticle-Induced Alteration of the Polarization State of Tumor-Associated Macrophages as a Substantial Antitumor Mechanism Against Oral Squamous Cell Carcinoma (OSCC).
Int J Nanomedicine
September 2025
Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology, Nanjing University, Nanjing, Jiangsu, People's Republic of China.
Introduction: Oral squamous cell carcinoma (OSCC) has a poor prognosis due to its immunosuppressive tumor microenvironment (TME), in which tumor-associated macrophages (TAMs) play a pivotal role in promoting disease progression and therapeutic resistance. This study examines whether Prussian blue nanoparticles (PB NPs) could reprogram TAMs and block tumor-stroma communication in OSCC.
Methods: PB NPs were synthesized using polyvinylpyrrolidone-assisted coprecipitation and characterized by transmission electron microscopy, dynamic light scattering, and UV-Vis spectroscopy.
Quantitative Structure-activity Relationship-based Neural Network Model in Screening Potential Inhibitors from Brown Algae against RelA in Oral Squamous Cell Carcinoma.
Int Dent J
September 2025
College of Dental Medicine, Roseman University of Health Sciences, South Jordan, Utah, USA; College of Graduate Studies, Roseman University of Health Science, South Jordan, Utah, USA. Electronic address:
Introduction And Aim: Oral squamous cell carcinomas (OSCCs) are one of the most frequently diagnosed head and neck cancers with a poor prognosis despite the advancements in diagnostic techniques and treatment strategies. The progression of OSCC is driven by several molecular mechanisms, among them the overexpression of transcription factor RelA, which plays a crucial role by correlating with the clinicopathological characteristics.
Methods: This systematic investigation focused on identifying the top 25 crucial molecular descriptors to predict the RelA inhibitor through the quantitative structure-activity relationship (QSAR)-based artificial neural network model.
Objective: This study aimed to elucidate the functional role and molecular mechanisms of Serine Peptidase Inhibitor Kazal Type 1 (SPINK1) in oral squamous cell carcinoma (OSCC) through integrative analysis of single-cell RNA sequencing (scRNA-seq) data.
Materials And Methods: Cellular subpopulations within OSCC were stratified using transcriptomic datasets from the GEO database. Cell-cell communication networks were reconstructed to map ligand-receptor interactions, while Gene Set Variation Analysis (GSVA) and Gene Set Enrichment Analysis (GSEA) were employed to systematically investigate SPINK1-associated signaling pathways.
[miR-302a-3p targeting lysosomal-associated membrane protein 5 inhibits the invasion and metastasis of oral squamous cell carcinoma].
Hua Xi Kou Qiang Yi Xue Za Zhi
August 2025
Oral & Maxillofacial Reconstruction and Regeneration of Luzhou Key Laboratory, Luzhou 646000, China.
Objectives: This study aimed to explore the expression of lysosomal-associated membrane protein 5 (LAMP5) and microRNA (miR)-302a-3p in oral squamous cell carcinoma (OSCC) and their functional mechanism on the invasion and metastasis of OSCC.
Methods: The expression of LAMP5 in OSCC and its sensitivity as a prognostic indicator were analyzed on the basis of The Cancer Genome Atlas database. Western blot, quantitative reverse transcription polymerase chain reaction, and cell immunocytochemistry were used to detect the expression of LAMP5 in OSCC tissues and cells.
Treatment response assessment in resistant oral squamous cell carcinoma applying F-FDG PET/CT with reference to the Lugano classification.
Oral Radiol
September 2025
Department of Oral and Maxillofacial Surgery, and Plastic Surgery, Gunma University Graduate School of Medicine, 3-39-22 Showa, Maebashi, Gunma, 371-8511, Japan.
Objective: The present study examined the efficacy of the F-FDG PET/CT (18F-FDG PET)-based Lugano Classification for Oral Cancer (Lugano-OC) to stage treatment-resistant oral cancer and its utility for predicting treatment responses.
Patients And Methods: We included 29 oral squamous cell carcinoma (OSCC) patients with disease progression post the primary treatment, who had F-FDG PET scans prior to secondary treatment initiation, and were evaluable via Lugano-OC between April 2013 and September 2022. To adapt Lugano-OC to the head and neck region, we designated the clavicle level, a regional lymph node in oral cancer, as the reference standard and visually evaluated 18F-FDG PET accumulation patterns.