Butyrate Reducing Bone Mass Loss by Regulating the Expression of m6A Methyltransferase METTL3 in Implant-Associated Staphylococcus aureus Osteomyelitis.

Staphylococcus aureus (S. aureus) has been identified as a hindrance to osteoblast differentiation, thereby contributing significantly to the development of osteomyelitis. Consequently, exploring pharmaceutical interventions targeting osteoblast differentiation mediated by S.

A nomogram for individualized prediction of acute respiratory distress syndrome in patients with severe traumatic brain injury: a retrospective cohort study.

Background: The prognosis of patients with a concomitance of severe traumatic brain injury (sTBI) and acute respiratory distress syndrome (ARDS) is poor, and early identification of such patients can provide diagnostic and therapeutic assistance for clinical treatment. However, few studies have been conducted to identify the risk of ARDS in patients with sTBI. This study aimed to construct a risk prediction model for ARDS in patients with sTBI and evaluate its efficacy.

Low serum 25 hydroxyvitamin D Is associated with severe metabolic dysfunction-associated steatotic liver disease in individuals with overweight/obesity: A cross-sectional study.

The role of vitamin D status in metabolic dysfunction-associated steatotic liver disease (MASLD) remains controversial. Furthermore, research evaluating the relationship between vitamin D status and abdominal fat distribution is limited. Therefore, we hypothesized that serum vitamin D is inversely associated with the severity of liver fat content (LFC) in populations with overweight/obesity.

Dual-Responsive Starch Hydrogels via Physicochemical Crosslinking for Wearable Pressure and Ultra-Sensitive Humidity Sensing.

Flexible hydrogel sensors demonstrate emerging applications, such as wearable electronics, soft robots, and humidity smart devices, but their further application is limited due to their single-responsive behavior and unstable, low-sensitivity signal output. This study develops a dual-responsive starch-based conductive hydrogel via a facile "one-pot" strategy, achieving mechanically robust pressure sensing and ultra-sensitive humidity detection. The starch-Poly (2,3-dihydrothieno-1,4-dioxin)-poly (styrenesulfonate) (PEDOT:PSS)-glutaraldehyde (SPG) hydrogel integrates physical entanglement and covalent crosslinking to form a porous dual-network architecture, exhibiting high compressive fracture stress (266 kPa), and stable electromechanical sensitivity (ΔI/I, ~2.

Toward preclinical evaluation of a thermosensitive PEG-poly(-alanine) hydrogel: A study on sterilization, storage stability, and in vivo performance.

Poly(amino acid)-based thermosensitive hydrogels hold great potential for clinical translation. Herein, we employ a thermosensitive methoxy poly(ethylene glycol)-block-poly(-alanine) (mPEG-PAla) hydrogel that undergoes a sol-to-gel transition upon heating as the model system to systematically evaluates its sterilizability, storage stability, in vivo degradation and in vivo drug release profiles-critical factors for clinical translation. mPEG-PAla copolymers are synthesized via ring-opening polymerization using the optimized amount of crown ether as the catalyst, ensuring controlled polymerization while minimizing catalyst usage.

Multi-omics integration reveals the impact of mediterranean diet on hepatic metabolism and gut microbiota in mice with metabolic dysfunction-associated steatotic liver disease.

Objective: To investigate the effects of the Mediterranean diet (MD) on hepatic metabolism and gut microbiota in mice with metabolic dysfunction-associated steatotic liver disease (MASLD).

Methods: C57BL/6 mice were fed a high-fat diet for 12 weeks to induce MASLD, with normal chow (NC)-fed mice as controls. Post-modeling, MASLD mice were randomized into three groups: HF (continued high-fat diet), HF-NC (switched to normal chow), and HF-MD (switched to MD).

Kapβ2 attenuates post-anesthetic neurocognitive dysfunction by resolving HnRNPA2/B1 fibrillary tangles in a rat model of mild cognitive impairment.

Background: Patients with mild cognitive impairment (MCI) before surgery have a higher incidence of perioperative neurocognitive disorders (PND) and a higher rate of progression to dementia than those without MCI; however, the underlying mechanisms are unclear. Heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNPA2/B1) is an RNA-binding protein (RBP) that forms fibrillary tangles via a steric zipper motif. Abnormal accumulation of HnRNPA2/B1 is strongly correlated with local neurodegeneration and cognitive impairment.

A Multi-Omics Data Integration Framework for Gene Regulatory Network Inference Based on Contrastive Learning.

The Gene Regulatory Networks (GRNs) ensure the stability of cellular states, preserving specific phenotypes and functions throughout the differentiation process. However, current tools still need improvement to effectively integrate multi-omics data and infer GRNs for particular cell types. We introduce CLMOGRI, a multi-omics TF-gene regulatory network inference framework based on heterogeneous networks and contrastive learning, designed to integrate multi-omics data for GRN inference.

Luminescence characterization and performance modulation of CaScBO: Eu near-infrared phosphors for night vision lighting applications.

To facilitate the development of near-infrared light sources for photovoltaic and night-vision lighting applications, it is crucial to obtain novel near-infrared emitting phosphors. Herein, a novel Eu-activated broadband near-infrared emitting phosphor CaScBO: Eu is reported. Under 558 nm light excitation, CaScBO: Eu exhibits broadband near-infrared emission centered at 780 nm with a full width at half maxima of 147 nm.

A multi-omics atlas of CAF subtypes reveals apCAF-M2 macrophage interactions driving immune resistance in glioma.

Cancer-associated fibroblasts (CAFs) are a critical component of the glioma microenvi-ronment and play essential roles in tumor progression and resistance to immunotherapy. To comprehensively characterize CAF heterogeneity and their interactions with immune cells, we conducted an integrative multi-omics analysis incorporating single-cell and bulk RNA sequencing, spatial transcriptomics, and multiplex immunofluorescence. This approach identified nine distinct CAF subtypes with phenotypic and functional diversity, including tumor-like CAFs (tCAFs), myofibroblast-like CAFs (myCAFs), vascular CAFs (vCAFs), metabolic CAFs (meCAFs), proliferative CAFs (pCAFs), antigen-presenting CAFs (apCAFs), interferon-responsive CAFs (infCAFs), inflammatory CAFs (iCAFs), and a group of CAFs with unknown identity.

Kapβ2 Reverses Sevoflurane-Induced Hydrogel Phase Transition of hnRNPA2/B1-SG in Hypoxic Primary Rat Hippocampal Neurons.

Aims: Sevoflurane can aggravate the progression of neurodegeneration, although the underlying mechanisms remain incompletely understood. Our previous study identified a link between heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNPA2/B1) and sevoflurane-induced neurocognitive impairments. The abnormal hydrogel phase transition of stress granules (SGs) assembled via liquid-liquid phase separation (LLPS) by hnRNPA2/B1 is a crucial element in neurodegeneration.

Emodin enhances host antiviral immunity against Micropterus salmoides rhabdovirus by activating RLR signaling in largemouth bass.

Micropterus salmoides rhabdovirus (MSRV) is a highly pathogenic virus that causes substantial mortality in largemouth bass, yet effective antiviral agents remain unavailable. In this study, we evaluated the antiviral potential of 20 plant-derived small molecules and identified emodin as the most effective inhibitor. At 25 mg/L, emodin reduced MSRV nucleoprotein gene expression in epithelioma papulosum cyprinid (EPC) cells by 93 %.

Preoperative ASAP score predicts recurrence and survival following curative hepatic resection for hepatitis B virus-related hepatocellular carcinoma: A multicenter cohort study.

Background & Aims: The ASAP score, integrating age, sex, alpha-fetoprotein (AFP), and protein induced by vitamin K absence or antagonist-II (PIVKA-II), has demonstrated value for early detection of hepatocellular carcinoma (HCC) in patients with chronic hepatitis B virus (HBV) infection. This study sought to assess whether the ASAP score could also predict recurrence and survival following curative resection of HBV-related HCC.

Methods: This multicenter study included patients undergoing curative hepatic resection for HBV-related HCC.

Characterizing immune and metabolic profiles in autism spectrum disorder through combined transcriptomics-metabonomics analysis.

Background: Autism Spectrum Disorders (ASD) encompass a range of complex neurodevelopmental conditions marked by difficulties in social communication, restricted interests, and repetitive behavior. In this study, we explore the potential interplay between metabolic and transcriptional alterations in ASD, aiming to uncover common biological disturbances that may contribute to the phenotype across cohorts.

Methods: Transcriptional and metabolomic data for ASD and typically developing (TD) samples were sourced from the GEO database.

Efficiency and Extent of Niacin-Induced Skin Flushing Patterns in Early Stages of Psychosis.

Niacin-induced skin flushing response (NSFR) attenuation is a well documented biomarker for psychosis and has also been used in studies of bipolar affective disorder. It appears not only in later stages but also in first episodes and in clinical high-risk (CHR) stages. NSFR tests were conducted on healthy controls (HC), CHR individuals, and first-episode psychosis (FEP) patients from January 2019 to March 2024.

ASFV pE146L-induced ER remodeling is essential for viral replication.

Unlabelled: African swine fever virus (ASFV) causes a severe hemorrhagic disease, posing a significant threat to the global pig industry. Although the ASFV encodes nearly 200 proteins, the functions of many remain unknown. Here, we identify the inner envelope protein pE146L as essential for ASFV replication.

Magnetic Sensing of Ion Transport in a Single Nanopore.

Developing magnetic measurement techniques for nanofluidic systems will unlock the access to the unexplored information on nanoconfined transport, which remains elusive. Herein, we integrate an atomic magnetometer with an artificial nanopore, constructing a magnetic sensing-based nanofluidic measurement system to profile nanoscale ion dynamics. Through magnetic noise suppression by a multilayer shielding system and configuration optimization with an optically pumped magnetometer, our system exhibits exceptional detection performances with a sensitivity reaching 12.

Robust EDTA-Functionalized MIL-101(Cr)-NH for Affinity-Enhanced Rare-Earth Ion Capture: Mechanistic Evaluation and Density Functional Theory Insights.

Sustainable extraction of rare-earth elements (REEs) from wastewater streams presents a critical technological challenge in resource recovery. This work developed a chelating metal-organic framework composite through ethylenediaminetetraacetic acid (EDTA) functionalization of MIL-101(Cr)-NH, demonstrating superior rare-earth ion affinity across light to heavy lanthanides (La, Ce, Gd, Dy). Under optimized aqueous conditions (pH ≈ 5.

The association between brominated flame retardants and serum testosterone levels in American adult men: NHANES 2013-2016.

Background: Brominated flame retardants (BFRs), especially polybrominated diphenyl ethers (PBDEs), are commonly utilized, yet their possible endocrine-disrupting effects have sparked significant concerns. Nonetheless, the link between exposure to BFRs and serum testosterone levels in adult males is still not well comprehended.

Methods: We analyzed data from 1,150 men aged ≥20 years from the National Health and Nutrition Examination Survey (NHANES) in 2013-2016.

Predictive efficacy and role of 2HG in MGMT promoter methylation in gliomas: a retrospective study based on MRS and mediator variable analysis.

Background: The role of 2-hydroxyglutarate (2HG) in the methylation process of O6-methylguanine-DNA methyltransferase (MGMT) promoter remains unclear. This study aimed to investigate the predictive efficacy and role of 2HG in MGMT promoter methylation.

Methods: Patients who met the inclusion criteria were retrospectively included and divided into MGMT promoter methylation group (M) and MGMT promoter unmethylation group (UM).

Investigating the efficacy of dipotassium glycyrrhizin emollients in adult atopic dermatitis: A focused randomized parallel-controlled clinical study.

Background & Objective: Atopic dermatitis (AD), a chronic inflammatory skin condition, is primarily characterized by persistent itching and significant sleep disruption. Dipotassium glycyrrhizin, known for its anti-inflammatory properties, has shown potential in AD treatment. This study aims to evaluate the effectiveness of emollients containing dipotassium glycyrrhiza extract and the impact of varying application methods on improving the clinical severity of atopic dermatitis in adults during remission periods.

Predictive role of erythrocyte fatty acids in conversion to psychosis: a longitudinal study of clinical high-risk individuals.

Introduction: Fatty acid (FA) alterations are associated in patients with psychosis. This study aimed to investigate the role of FAs in predicting the conversion to psychosis in individuals at clinical high-risk (CHR) and to distinguish CHR individuals from healthy controls (HC).

Materials And Methods: A three-year longitudinal study was conducted involving 67 CHR individuals and 25 HC participants.

High-Coverage Profiling of Hydroxyl and Amino Compounds in Sauce-Flavor Baijiu Using Bromine Isotope Labeling and Ultra-High Performance Liquid Chromatography-High-Resolution Mass Spectrometry.

: Hydroxyl and amino compounds play a significant role in defining the flavor and quality of sauce-flavor Baijiu, yet their comprehensive analysis remains challenging due to limitations in detection sensitivity. In this study, we developed a novel bromine isotope labeling approach combined with ultra-high performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS) to achieve high-coverage profiling of these compounds in sauce-flavor Baijiu. : The method employs 5-bromonicotinoyl chloride (BrNC) for rapid (30 s) and mild (room temperature) labeling of hydroxyl and amino functional groups, utilizing bromine's natural isotopic pattern (Δ/ = 1.

Mechanistic Insights into TSH-Mediated Macrophage Mitochondrial Dysfunction via TSHR Signaling in Metabolic Disorders.

The role of thyroid-stimulating hormone receptor (TSHR) in macrophages on adipose tissue metabolic disorders remains unclear. We generated macrophage-specific TSHR knockout mice (LysM-Cre Tshr) using the Cre/LoxP system and induced subclinical hypothyroidism (SCH) via methimazole treatment. Metabolic/inflammatory phenotypes were assessed via glucose/insulin tolerance tests, histology, and molecular analyses.

Novel Equivalent Carbon Number Strategy for Large-Scale Lipidomics Data Analysis via Ultrahigh-Performance Liquid Chromatography-Orbitrap Astral Mass Spectrometry.

Lipidomics enables studying lipid alterations in physiological or pathological states. The new Orbitrap mass spectrometry (MS), equipped with an Astral analyzer, significantly increases MS/MS acquisition rate. However, simultaneous analysis of lipid retention behavior and structural annotation from this data remains challenging.