Emergence of carbapenem-resistant Mbandaka through IS-driven mobilization and chromosomal structural variation.

subsp. serovar Mbandaka (. Mbandaka) represents an emerging public health threat due to its remarkable genomic adaptability, involvement in multinational foodborne outbreaks, and the ability to establish persistent infections.

Epidemiology and resistance mechanisms of tigecycline- and carbapenem-resistant in China: a multicentre genome-based study.

Objectives: To elucidate the molecular epidemiology of tigecycline and carbapenem-resistant isolates and mechanisms of tigecycline resistance.

Methods: We gathered 31 unduplicated strains of tigecycline-resistant from six hospitals nationwide. Antimicrobial susceptibility testing, phenotypic detection, and PCR identification were performed first, followed by homology analysis using MLST and PFGE.

Establishing Sitafloxacin Epidemiological Cut-off Values (ECOFFs) for Clinical Bacterial Isolates.

Objective: To establish the epidemiological cut-off values (ECOFFs) of sitafloxacin against , and

Methods: We collected 2264 clinical isolates from five different labs located in four cities in China. The minimum inhibitory concentrations (MICs) and inhibition zone diameters of sitafloxacin for all isolates were determined by using the broth microdilution method (BMD) and the disk diffusion method according to European Committee on Antimicrobial Susceptibility Testing (EUCAST) guidelines. MIC ECOFFs were determined using ECOFFinder software, with the 99% calculated ECOFF selected as the initial value.

A tympanitis-related brain abscess caused by Helcococcus kunzii in China: a case report and literature review.

Background: Infections attributed to Helcococcus kunzii are rarely documented, especially in relation to brain abscesses. This study aims to report the first documented case of a brain abscess associated with tympanitis caused by H. kunzii in China, alongside a comprehensive review of the existing literature.

Peptoniphilus vaginalis bacteremia in a patient with diabetic foot infection: First reported case and literature review.

Background: Peptoniphilus vaginalis, a Gram-positive anaerobic coccus, is a recently characterized species within the Peptoniphilus genus. No studies have detailed its features. This report documents the first case of P.

Contaminated faucets and sinks as a reservoir for antibiotic-resistant bacterial transmission in healthcare settings.

Introduction: Multidrug-resistant bacterial infection has emerged as a global hygiene threat in hospitals, and outbreaks cause increased patient morbidity and mortality in the healthcare system. Mounting evidence points to faucets and sinks as the culprits in the outbreaks of multidrug-resistant bacterial infections. However, the mechanism and the route through which faucets and sinks contribute to antibiotic-resistant bacterial transmission are not fully understood.

Secular trends of physical fitness for college students in Anhui Province over the past decade.

Background: Since the beginning of the 21st century, China's economy has experienced rapid growth, resulting in a steady improvement in its citizens' living standards. However, alongside the emergence of modern civilization-related health issues, the overall physical fitness of the population has been declining. In the final year of 2019, a global COVID-19 pandemic emerged and persisted for three years, causing a significant diminution in human physical well-being.

Establishment of epidemiological cut-off values of etimicin: a new fourth-generation aminoglycoside, against Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, Acinetobacter baumannii and Staphylococcus aureus.

Objective: To determine the epidemiological cut-off (ECOFF) values of etimicin against Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, Acinetobacter baumannii and Staphylococcus aureus.

Methods: We selected 1500 isolates from five hospitals throughout five cities in China spanning from January 2018 to December 2021 in the study. Minimal inhibit concentrations (MICs) of etimicin were determined using the broth microdilution method.

Active mechanism of "Zishen Huayu Fang" in treating intrauterine adhesions based on network pharmacology and macromolecular docking.

The study is investigating the mechanism of therapeutics of "Zishen Huayu Fang" in the adhesions of intrauterine. Pharmacology systems have been employed as active ingredients of 131 compounds like Luteolin, Quercetin and Kaempferol in this research study in a combination of other compounds to prove the efficacy of the Zishen Huayu Fang in the treatment of adhesions inside the uterus. The study aims to understand how the ingredients in "Zishen Huayu Fang" interact with the body, particularly how they can assist in the prevention, mitigation and elimination of adhesions inside the uterus.

Can heat conditions affect the heart rate responses, perception of effort, and technical performance of young male football players during small-sided games? a comparative study.

Background: Soccer coaches often employ small-sided games (SSGs) to elicit both physiological and technical responses from players. However, numerous contextual factors can influence the outcomes of these games. This comparative study aimed to investigate how environmental temperature (< 21ºC and > 29ºC) impacts heart rate responses, perception of effort, and technical performance in young male football players during SSGs.

Modification of carbapenemase inhibition test and comparison of its performance with NG-Test CARBA 5 for detection of carbapenemase-producing Enterobacterales.

Aims: Adequately and accurately identifying carbapenemase-producing Enterobacterales (CPE) is vital for selecting appropriate antimicrobial therapy and implementing effective infection control measures. This study aims to optimize the phenotypic detection method of carbapenemase for routine diagnostics in clinical microbiology laboratories.

Methods And Results: Carbapenemase genes in 2665 non-duplicate CRE clinical strains collected from various regions of China were confirmed through whole-genome sequencing (WGS).

AWD-stacking: An enhanced ensemble learning model for predicting glucose levels.

Accurate prediction of blood glucose levels is essential for type 1 diabetes optimizing insulin therapy and minimizing complications in patients with type 1 diabetes. Using ensemble learning algorithms is a promising approach. In this regard, this study proposes an improved stacking ensemble learning algorithm for predicting blood glucose level, in which three improved long short-term memory network models are used as the base model, and an improved nearest neighbor propagation clustering algorithm is adaptively weighted to this ensemble model.

Using the Paired Sample t test to Analyze the Effect of Jogging on the Cardiovascular Function of College Students.

Background: In China, there have been instances of sudden cardiac death among university students, with a significant number of students being at risk of cardiovascular diseases. This risk is often attributed to sub-health conditions such as weight gain and obesity, which are triggered by sedentary lifestyles, irregular living habits, and unregulated diets. Therefore, it is crucial to enhance the guidance for participation in physical activities, encouraging students to actively reduce their risk of cardiovascular diseases (CVD).

Typhimurium with Eight Tandem Copies of on a HI2 Plasmid.

Carbapenem-resistant has recently aroused increasing attention. In this study, a total of four sequence type 36 subsp. serovar Typhimurium ( Typhimurium) isolates were consecutively isolated from an 11-month-old female patient with a gastrointestinal infection, of which one was sensitive to carbapenems and three were resistant to carbapenems.

Microsatellite markers for genotyping of Kodamaea ohmeri: Demonstrating outbreaks based on a multicenter surveillance study in China.

Kodamaea ohmeri, an emerging human pathogen, caused both sporadic and nosocomial infections among immunocompromised people with high mortality. However, there is limited research on the molecular epidemiology of K. ohmeri.

Comparing the broth enrichment-multiplex lateral flow immunochromatographic assay with real time quantitative PCR for the rapid detection of carbapenemase-producing organisms in rectal swabs.

Background: Rapid and accurate identification of carbapenemase-producing organism (CPO) intestinal carriers is essential for infection prevention and control. Molecular diagnostic methods can produce results in as little as 1 h, but require special instrumentation and are expensive. Therefore, it is urgent to find an alternative method.

Diagnosis of fungal keratitis caused by Nectria haematococca through next-generation sequencing: review of literature and report of three cases.

Objectives: Fungal keratitis (FK) is a kind of serious corneal infection and penetrating keratoplasty (PKP) is needed when medical therapy fails. Although Nectria haematococca is found as endophytes in the roots of some plant species, there has been no report of N. haematococca infection in human.

A multicenter investigation of 2,773 cases of bloodstream infections based on China antimicrobial surveillance network (CHINET).

Background: Bloodstream infections (BSIs), especially hospital-acquired BSIs, are a major cause of morbidity and mortality. However, the details about the pathogens and antimicrobial resistance profile of BSIs across China are still lacking.

Methods: An investigation was conducted in 10 large teaching hospitals from seven geographic regions across China in 2016 based on China Antimicrobial Surveillance Network (CHINET) to profile the clinical and etiological features of BSIs.

Decreased echinocandin susceptibility in causing candidemia and emergence of a pan-echinocandin resistant case in China.

is becoming a predominant non- cause of invasive candidiasis (IC). Echinocandins are the preferred choice for IC treatment and prophylaxis. Resistance to echinocandins in has emerged in several countries, but little is known about the susceptibility profile in China or about mechanisms of resistance.

Case report: infection during ICI therapy in a hepatocellular carcinoma patient with psoriasis.

We report here a patient with advanced hepatocellular carcinoma (HCC) and psoriasis treated with immune checkpoint inhibitor (ICI) therapy who experienced tumor partial response and psoriatic exacerbation. Meanwhile, the patient contracted during the treatment period, while it was an opportunistic infection and mainly happened in immunosuppressed patients. We discussed the possibility that this infection was an ICI-associated infection independent of immunosuppression due to dysregulated immunity, which was the result of the effects of immunotherapy and autoimmune disease (AID), and the characteristics and treatment of , which was rarely reported in China.

Emergence of High-Level Cefiderocol Resistance in Carbapenem-Resistant Klebsiella pneumoniae from Bloodstream Infections in Patients with Hematologic Malignancies in China.

Cefiderocol is a novel siderophore cephalosporin exhibiting potent antimicrobial activities. Although cefiderocol has not been approved in China, resistance is emerging. A multicenter study was performed to evaluate the cefiderocol resistance in carbapenem-resistant Klebsiella pneumoniae (CRKP) strains from bloodstream infections in patients with hematologic malignancies in China.

Pythium insidiosum keratitis reported in China, raising the alertness to this fungus-like infection: a case series.

Background: The objective of this study is to report typical clinical and laboratory characteristics of three cases of keratitis caused by Pythium insidiosum in China.

Case Presentation: Three Chinese patients of Han nationality diagnosed with Pythium keratitis from 2017 to 2019 were included. One 45-year-old female and one 55-year-old male were exposed to river water, and one 51-year-old female was burned by ash in the eyes.

Alcaligenes faecalis metallo-β-lactamase in extensively drug-resistant Pseudomonas aeruginosa isolates.

Objectives: To characterize Alcaligenes faecalis metallo-β-lactamase (MBL) AFM-2 and AFM-3 from clinical Pseudomonas aeruginosa isolates NDTH10366, NDTH9845 and WTJH17.

Methods: Clinical isolates were whole-genome sequenced using the Illumina and Oxford Nanopore platforms. MICs of clinical isolates and transformants containing MBL genes were determined using broth microdilution methods.

Antifungal Susceptibility Profiles and Resistance Mechanisms of Clinical Isolates With High MIC Values.

() is an ascomycete yeast species widely used in environmental and industrial research and capable of causing infections in humans and animals. At present, there are only a few studies on , and further research is required for its more in-depth characterization and analysis. Eleven strains of collected from China Hospital Invasive Fungal Surveillance Net (CHIF-NET) and the CHIF-NET North China Program were identified using matrix-assisted laser desorption ionization-time of flight mass spectrometry and internal transcribed spacer sequencing.