Extraintestinal pathogenic Escherichia coli (ExPEC) O-serotype distribution and antibiotic resistance profiles among the aging population in China: A longitudinal, multi-center study.

Extraintestinal pathogenic Escherichia coli (ExPEC) is a leading cause of community-acquired bacteremia and sepsis, which contributes to the substantial burden of invasive E. coli disease (IED) in older adults. This study aimed to estimate the O-serotype distribution of blood and sterile site ExPEC among older adults in China and the characteristics of antimicrobial resistance, O-serotypes, and O genotypes.

Repurposing Mercaptopurine Through Collateral Lethality to Treat Cancers with Somatic Loss.

Somatic retinoblastoma 1 () loss is prevalent across different cancer types and is enriched in treatment-refractory tumors, such as castration-resistant prostate cancer (CRPC) and small-cell lung cancer, but cannot be considered as a direct druggable target. In this study, we revealed that the close proximity of nudix hydrolase 15 () and may result in their common somatic codeletion or epigenomic cosilencing in different cancer types and subsequent significant positive correlations of their expressions at the bulk transcriptional and single-cell levels. With clinical CRPC samples, co-loss of and were commonly observed (14 out of 21).

Numerical Analysis of Gravel Packing in Water Control Completion for Horizontal Wells: Mathematical Characterization and Experimental Validation.

Horizontal well technology has emerged as a key approach in oil and gas development, particularly for addressing sand production and water breakthrough in offshore reservoirs. To improve the design and efficiency of gravel packing in water control completions, a novel two-dimensional, time-dependent numerical model is developed to simulate slurry flow behavior in horizontal sections. The model accounts for the coupled flow in the screen-wellbore and screen-base pipe annuli, incorporating governing equations for momentum, mass conservation, formation filtration, and gravel settling.

Molecular Mechanism of Metformin Regulating the Regeneration of Planarian Through .

Metformin is one of the most commonly used medications to treat type 2 diabetes. In addition to lowering blood sugar, it can also promote the regeneration of certain organs or tissues. Planarian , with its remarkable regenerative capacity, has become an important model organism for studying pharmacology and regenerative medicine.

In vitro activity of the novel β-lactamase inhibitor FL058 combined with meropenem against KPC- or NDM-producing enterobacterales and Pseudomonas aeruginosa.

Purpose: Carbapenem-resistant organisms pose a significant public health threat due to high mortality and limited treatment options. This study aimed to evaluate the in vitro antimicrobial activity of the novel β-lactamase inhibitor FL058 combined with meropenem against carbapenem-resistant Enterobacterales and Pseudomonas aeruginosa clinical isolates.

Methods: The MICs of 85 clinical isolates, including KPC- or NDM-producing Enterobacterales and carbapenem-resistant/susceptible P.

Genome-wide identification of key genes related to chloride ion (Cl) channels and transporters in response to salt stress in birch.

Background: Soil salinization is a common matter of concern all over the world, which severely affects plant production, soil health, and ecosystem stability. Birch is a significant afforestation tree species with great ecological, economic, and evolutionary value. It is an excellent model system for studying the acclimation and adaptation of woody plants to extreme environmental conditions due to the current advancements in genomics, genetic variability, and extensive studies with a focus on various biotic and abiotic stresses.

New insights into the pharmacological mechanisms of Jinqi Jiangtang Tablets in the treatment of type 2 diabetes mellitus: A multi-omics approach combined with experimental validation.

Ethnopharmacological Relevance: Type 2 diabetes mellitus (T2DM) is a prevalent chronic metabolic disorder characterized by impaired glucose homeostasis and insulin dysfunction. Despite ongoing efforts, the management of T2DM remains challenging due to its complex pathogenesis and the limited effectiveness of current treatments. Jinqi Jiangtang Tablets (JQJTT), a well-established Traditional Chinese Medicine (TCM) formula, have shown promising clinical benefits in regulating blood glucose levels and improving metabolic health in T2DM patients.

Antimicrobial activity of lefamulin against pathogens most commonly causing community-acquired pneumonia: results from the China antimicrobial surveillance network in 2020-2022.

Objectives: Lefamulin is a novel pleuromutilin antibiotic used for the treatment of community-acquired pneumonia (CAP). This study aimed to evaluate the in vitro antimicrobial activity of lefamulin against clinical isolates obtained from China.

Methods: 1,052 non-duplicate isolates included the following isolates: Streptococcus pneumoniae (n = 529), Staphylococcus aureus (n = 306), Haemophilus influenzae (n = 121), Moraxella catarrhalis (n = 81), and Mycoplasma pneumoniae (n = 15), were collected from 70 hospitals participating in the China Antimicrobial Surveillance Network (CHINET) between October 1, 2020, and November 30, 2022.

Characterization of Mechanical Property Evolution and Durability Life Prediction of Engineered Cementitious Composites Under Frozen State.

Engineered cementitious composites (ECCs) exhibit superior mechanical properties (MPs) and excellent crack control capabilities, making them widely used in practical engineering applications. However, the MPs of ECCs in frozen states (FSs), particularly their flexural properties (FPs), still need to be better understood. MP tests were designed for frozen ECC samples to investigate the service performance of ECCs in an FS.

MYLK-AS1 Enhances Glutamine Metabolism to Promote EGFR Inhibitor Resistance in Non-Small Cell Lung Cancer.

Unlabelled: Acquired resistance to EGFR tyrosine kinase inhibitors (TKI) limits the efficacy of molecular targeted therapy in non-small cell lung cancer (NSCLC). Metabolic reprogramming is a hallmark of lung cancer that could contribute to TKI resistance. Through systematic screening and verification, we identified a role for the long noncoding RNA MYLK-AS1 supporting acquired TKI resistance in lung cancer.

Transcriptome Sequencing Analysis of the Effects of Metformin on the Regeneration of Planarian .

Background: Metformin is a widely used oral hypoglycemic agent for treating type 2 diabetes. Planarians, with their remarkable regenerative abilities, are frequently employed as model organisms in stem cell and regeneration studies. This study aimed to investigate the effects of metformin on planarian regeneration, focusing on the regeneration of eyespots after amputation.

Clinical, genetic and functional perspectives on ATP-binding cassette subfamily B member 4 variants in five cholestasis adults.

Background: ATP-binding cassette subfamily B member 4 () deficiency is associated with cholestatic liver disease primarily because of missense mutations, and many variants remain unidentified. Here, we validate the pathogenicity and mechanism of ABCB4 variants in clinical and trials, hypothesizing that these variants are responsible for impaired biliary function and contribute to the development of cholestatic liver diseases.

Aim: To clarify the functional features and pathogenicity of variants.

NNMT promotes acquired EGFR-TKI resistance by forming EGR1 and lactate-mediated double positive feedback loops in non-small cell lung cancer.

Background: Epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) are remarkably effective for treating EGFR-mutant non-small cell lung cancer (NSCLC). However, patients inevitably develop acquired drug resistance, resulting in recurrence or metastasis. It is important to identify novel effective therapeutic targets to reverse acquired TKI resistance.

Modified FeO Nanoparticles for Foam Stabilization: Mechanisms and Applications for Enhanced Oil Recovery.

Nanoparticles (NPs) have shown great potential in stabilizing foam for enhanced oil recovery (EOR). However, conventional NPs are difficult to recover and may contaminate produced oil, increasing operational costs. In contrast, superparamagnetic FeO NPs can be efficiently recovered using external magnetic fields, offering a sustainable solution for foam stabilization.

Elimination of Concentration Polarization Under Ultra-High Current Density Zinc Deposition by Nanofluid Self-Driven Ion Enrichment.

The commercialization of zinc metal batteries aims at high-rate capability and lightweight, which requires zinc anodes working at high current density, high areal capacity, and high depth of discharge. However, frequent zinc anode fades drastically under extreme conditions. Herein, it is revealed that the primary reason for the anode instability is the severe concentration polarization caused by the imbalanced consumption rate and transfer rate of Zn under extreme conditions.

8q24 derived ZNF252P promotes tumorigenesis by driving phase separation to activate c-Myc mediated feedback loop.

As a well-known cancer risk region, the 8q24 locus is frequently amplified in a variety of solid tumors. Here we identify a pseudogene-derived oncogenic lncRNA, ZNF252P, which is upregulated in a variety of cancer types by copy number gain as well as c-Myc-mediated transcriptional activation. Mechanistically, ZNF252P binds and drives "phase separation" of HNRNPK and ILF3 protein in the nucleus and cytoplasm, respectively, to transcriptionally and posttranscriptionally activate c-Myc, thus forming a c-Myc/ZNF252P/c-Myc positive feedback loop.

STK11 genetic alterations in metastatic EGFR mutant lung cancer.

This study was conducted to investigate the relationship between STK11 genetic alterations and the outcomes of patients with metastatic EGFR mutant lung cancer. Clinical characteristics and genomic data were downloaded from the cBioPortal database. The information of the case with STK11 mutation was collected from Jiangyin People's Hospital.

Rheological Properties of Weak Gel System Cross-Linked from Chromium Acetate and Polyacrylamide and Its Application in Enhanced Oil Recovery After Polymer Flooding for Heterogeneous Reservoir.

Long-term polymer flooding exacerbates reservoir heterogeneity, intensifying intra- and inter-layer conflicts, which makes it difficult to recover the remaining oil. Therefore, further improvement in oil recovery after polymer flooding is essential. In this study, a weak gel system was successfully synthesized, and possesses a distinct network structure that becomes more compact as the concentration of partially hydrolyzed polyacrylamide increases.

Adaptive evolution of carbapenem-resistant hypervirulent in the urinary tract of a single patient.

The emergence of carbapenem-resistant hypervirulent (CR-hvKp) is a growing concern due to its high mortality and limited treatment options. Although hypermucoviscosity is crucial for CR-hvKp infection, the role of changes in bacterial mucoviscosity in the host colonization and persistence of CR-hvKp is not clearly defined. Herein, we observed a phenotypic switch of CR-hvKp from a hypermucoviscous to a hypomucoviscous state in a patient with scrotal abscess and urinary tract infection (UTI).

Modification of carbapenemase inhibition test and comparison of its performance with NG-Test CARBA 5 for detection of carbapenemase-producing Enterobacterales.

Aims: Adequately and accurately identifying carbapenemase-producing Enterobacterales (CPE) is vital for selecting appropriate antimicrobial therapy and implementing effective infection control measures. This study aims to optimize the phenotypic detection method of carbapenemase for routine diagnostics in clinical microbiology laboratories.

Methods And Results: Carbapenemase genes in 2665 non-duplicate CRE clinical strains collected from various regions of China were confirmed through whole-genome sequencing (WGS).

Uncovering the action mechanism of Shenqi Tiaoshen formula in the treatment of chronic obstructive pulmonary disease through network pharmacology, molecular docking, and experimental verification.

Objective: To reveal the potential underlying mechanism of the Shenqi Tiaoshen formula (, SQTS) in the treatment of chronic obstructive pulmonary disease (COPD) by utilizing network pharmacology, molecular docking, and experimental verification.

Methods: Multiple open-source databases and research related to Traditional Chinese Medicine or compounds were employed to screen active ingredients and corresponding potential targets of the SQTS. The protein-protein interaction network screened hub genes, the relevant molecular mechanism and gene regulation were initially identified through the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) analysis, and molecular docking was used to confirm further the interaction of the main components bound to the core targets.

Evaluation of the in vitro activity of ampicillin-sulbactam and cefoperazone-sulbactam against A. Baumannii by the broth disk elution test.

To evaluate the in vitro activity of ampicillin-sulbactam and cefoperazone-sulbactam against A. baumannii using the broth disk elution testing, a total of 150 A. baumannii isolates were collected from across China between January 2019 and January 2021, including 51 carbapenem-susceptible and 99 carbapenem-resistant isolates.

Complex evolutionary trajectories in vivo of two novel KPC variants conferring ceftazidime-avibactam resistance.

More and more ceftazidime-avibactam-resistant KPC-producing Klebsiella pneumoniae have been reported with its widespread use, and the detection rate of KPC variants has increased dramatically. However, the evolutionary mechanism and fitness effects during KPC mutation remained unknown. Here, we report the complex in vivo evolutionary trajectories of two novel KPC variants, KPC-155 (L169P/GT242A) and KPC-185 (D179Y/GT242A), from K.