Activity-dependent phosphorylation of CDYL by CDK5 regulates fear memory in mice.

Fear memory is crucial for animals to effectively respond to dynamic environments and survive dangerous stimuli. However, aberrant fear memory contributes to various psychiatric disorders, such as post-traumatic stress disorder (PTSD). Despite its importance, the precise molecular mechanisms underlying fear memory remain insufficiently understood.

Contrast-enhanced ultrasound in evaluating the curative effect of interventional therapy in patients with liver cancer.

Background: Contrast-enhanced ultrasonography (CEUS) is utilized to assess the therapeutic efficacy of interventional therapy in liver cancer patients, offering insights into tumor blood flow changes, angiogenesis, and tumor markers.

Aim: To evaluate the use of CEUS in examining the effectiveness of interventional therapy for liver cancer, we aim to investigate its diagnostic utility for tumor perfusion patterns, microvessel density, perfusion recovery, blood flow enhancement response, and alterations in tumor markers among patients receiving interventional therapy for liver cancer.

Methods: The study involved 124 patients who underwent interventional therapy for liver cancer at Guangzhou First People's Hospital from January 2022 to February 2024.

Engineering a biocompatible chitosan/MOF aerogel for hyperbilirubinemia treatment in liver failure.

Liver failure, marked by a rapid or progressive decline in liver function, leads to complications such as hyperbilirubinemia and infections, contributing to approximately 2 million global deaths each year. Current hemoperfusion methods for hyperbilirubinemia treatment are limited by their low adsorption selectivity and inadequate biosafety, necessitating better treatments. In this study, a chitosan-based HKUST-1 aerogel (HC) was developed as a highly selective, biosafe, and antibacterial adsorbent.

Development of sodium alginate-based active packaging films reinforced with curcumin/β-cyclodextrin stabilized high internal phase Pickering emulsions for blueberry preservation.

Maintaining the safety and quality of minimally processed agricultural products is a significant challenge in modern food supply chains. This study developed multifunctional sodium alginate (SA)-based films incorporating high internal phase Pickering emulsions (HIPPEs) stabilized by curcumin/β-cyclodextrin inclusion complexes (CUR/β-CD). The HIPPEs demonstrated excellent colloidal stability (>21 days) with a mean droplet diameter of 12.

Endothelial Activation Stress Index predicts all-cause and cardiovascular mortality in patients with diabetes: A nationwide study.

Introduction: Evidence suggests that the Endothelial Activation and Stress Index (EASIX) predicts mortality in endothelium-related conditions, but its link to mortality risk in diabetes remains unclear. This study investigates the association between EASIX and mortality risk in diabetes patients.

Methods: We included 3,252 diabetes patients from seven National Health and Nutrition Examination Survey cycles (2003-2016).

Unveiling the Spatiotemporal Dynamics of Global Brain Circulation: A Comprehensive Corpus (2000-2024).

The global competition for human capital is fuelled by intricate brain circulation dynamics, where individuals with specialized skills traverse geographic, organizational, and national boundaries to address workforce demands. However, a comprehensive framework for integrating and interpreting heterogeneous data on global brain circulation remains elusive. Here we introduce the Global Brain Circulation Dynamics (GBCD) corpus, a longitudinally integrated repository of geo-information encompassing 223 countries/regions from 2000 to 2024.

Core-shell structured gold nanoparticle-AIEgen nanohybrids for enhanced dual-mode lateral flow immunoassay of fumonisin B1.

Herein, we report the design and synthesis of bifunctional colorimetric and fluorescent nanohybrids (Au-AIENPs) through the co-assembly of oleylamine-modified gold nanoparticles (OA-AuNPs) and aggregation-induced emission luminogens (AIEgen). Due to the distinct compatibility of the components, the resulting Au-AIENPs form a core-shell nanostructure, with the AIEgen creating a fluorescent core and the OA-AuNPs uniformly dispersing on the shell. This spatial separation of AIEgen and OA-AuNPs optimizes the colorimetric signal while preserving a strong fluorescence output.

Magnesium-L-threonate Ameliorates Cognitive Deficit by Attenuating Adult Hippocampal Neurogenesis Impairment in a Mouse Model of Alzheimer's Disease.

Impaired adult hippocampal neurogenesis is a key pathological mechanism contributing to memory deficits in Alzheimer's disease (AD). Recent studies have shown that elevating magnesium levels promotes neurogenesis by enhancing the neuronal differentiation of adult neural progenitor cells in vitro. Therefore, this in vivo study aims to determine if magnesium-L-threonate (MgT) can ameliorate cognitive deficit of AD mice by attenuating adult hippocampal neurogenesis impairment and to reveal the underlying mechanisms.

Effect of ultrasound combined with microbubbles therapy on tumor hypoxic microenvironment.

Introduction: Tumor tissues exhibit significantly lower oxygen partial pressure compared to normal tissues, leading to hypoxia in the tumor microenvironment and result in resistance to tumor treatments. Strategies to mitigate hypoxia include enhancing blood perfusion and oxygen supply, for example,by decomposing hydrogen peroxide within the tumor. Improving hypoxia in the tumor microenvironment could potentially improve the efficacy of cancer treatments.

Konjac glucomannan/zein active film loaded with tea polyphenol-ferric nanoparticles for strawberry preservation.

With increasing global environmental awareness and concerns about food safety, biodegradable active packaging has garnered widespread attention. In this study, the stability and bioactivity of tea polyphenol (TP) were enhanced through the preparation of TP-ferric nanoparticles (TP-Fe NPs) using metal-polyphenol ion coordination. Moreover, the introduction of Fe ions can further enhance the antibacterial effects of TP-Fe NPs.

Dual-Enzyme-Instructed Peptide Self-Assembly to Boost Immunogenic Cell Death by Coordinating Intracellular Calcium Overload and Chemotherapy.

The concept of immunogenic cell death (ICD) induced by chemotherapy as a potential synergistic modality for cancer immunotherapy has been widely discussed. Unfortunately, most chemotherapeutic agents failed to dictate effective ICD responses due to their defects in inducing potent ICD signaling. Here, we report a dual-enzyme-instructed peptide self-assembly platform of (CPT-GFFpY-PLGVRK-Caps) that cooperatively utilizes camptothecin (CPT) and capsaicin (Caps) to promote ICD and engage systemic adaptive immunity for tumor rejection.

Deep source transfer learning for the estimation of internal brain dynamics using scalp EEG.

Electroencephalography (EEG) provides high temporal resolution neural data for brain-computer interfacing via noninvasive electrophysiological recording. Estimating the internal brain activity by means of source imaging techniques can further improve the spatial resolution of EEG and enhance the reliability of neural decoding and brain-computer interaction. In this work, we propose a novel EEG data-driven source imaging scheme for precise and efficient estimation of macroscale spatiotemporal brain dynamics across thalamus and cortical regions with deep learning methods.

Comparison of lenvatinib plus pembrolizumab versus first-line systemic chemotherapy for advanced intrahepatic cholangiocarcinoma: a real-world retrospective study.

Background: Systemic chemotherapy (SC) stands the only first-line treatment for advanced intrahepatic cholangiocarcinoma (iCCA) for the past few decades. Immune checkpoint inhibitors (ICIs) have been proved to provide additional benefit in disease control. However, oncological outcome of iCCA remains poor and awaits further improvement with new treatment modalities.

Exosomes derived from cardiac fibroblasts with Ang-II stimulation provoke myocardial hypertrophy via miR-15b-5p/PTEN-L axis.

This study aimed to examine the impact of exosomes derived from Ang II-stimulated cardiac fibroblasts (CFs) on myocardial hypertrophy. Neonatal rat CFs were isolated and identified using Vimentin immunofluorescence. Following Ang II stimulation, exosomes were collected, characterized, and subjected to miRNA sequencing.

The effect of unaffected side resistance training on upper limb function reconstruction and prevention of sarcopenia in stroke patients: a randomized controlled trial.

Aging has made stroke a top killer and disabler, with post-stroke sarcopenia worsening disability and quality of life. While resistance training benefits the elderly, its impact on stroke patients is understudied. This study evaluates the potential of a 4-week unilateral resistance training (URT) program to prevent sarcopenia in stroke patients.

Neutrophil-lymphocyte ratio is a predictor for all-cause and cardiovascular mortality in individuals with prediabetes in a National study.

Purpose: We aimed to investigate the value of the neutrophil-lymphocyte ratio (NLR) in predicting the all-cause and cardiovascular mortality risk of individuals with prediabetes.

Methods: A total of 11,504 prediabetic patients from the National Health and Nutrition Examination Survey (NHANES) 2003-2016 were included in the present study. Mortality and the underlying cause of death were ascertained by linkage to National Death Index records through December 31, 2019.

Sequential vs. concurrent systemic therapies in combination with FOLFOX-HAIC for locally advanced hepatocellular carcinoma: a single-center, real-world cohort study.

Background: Tri-combination therapy based on hepatic arterial infusion chemotherapy (HAIC) of infusion fluorouracil, leucovorin, and oxaliplatin (FOLFOX-HAIC) plus immune checkpoint inhibitors (ICIs) and tyrosine kinase inhibitors (TKIs) for the locally advanced hepatocellular carcinoma (HCC) patients have been proven effective. However, whether it was best for these HCC patients to start with the most potent therapeutic pattern was still under debate. This retrospective study evaluated the efficacy and safety of FOLFOX-HAIC combined with systemic therapies in the patterns of sequential and concurrent schedules.

Tumor perfusion enhancement by microbubbles ultrasonic cavitation reduces tumor glycolysis metabolism and alleviate tumor acidosis.

The tumor microenvironment is increasingly acknowledged as a critical contributor to cancer progression, mediating genetic and epigenetic alterations. Beyond diverse cellular interactions from the microenvironment, physicochemical factors such as tumor acidosis also significantly affect cancer dynamics. Recent research has highlighted that tumor acidosis facilitates invasion, immune escape, metastasis, and resistance to therapies.

Ultrasensitive dynamic light scattering immunodetection of alpha-fetoprotein using heptamer-amplified nanoparticle crosslinking aggregation.

A novel construction strategy is introduced for an ultrasensitive dynamic light scattering (DLS) immunosensor targeting alpha fetoprotein (AFP). This approach relies on a self-assembled heptamer fusion protein (A1-C4bpα), incorporating the dual functions of multivalent recognition and crosslinking aggregation amplification due to the presence of seven AFP-specific A1 nanobodies on the A1-C4bpα heptamer. Leveraging antibody-functionalized magnetic nanoparticles for target AFP capture and DLS signal output, the proposed heptamer-assisted DLS immunosensor offers high sensitivity, strong specificity, and ease of operation.

Enzyme-Instructed Photoactivatable Supramolecular Antigens on Cancer Cell Membranes for Precision-Controlled T-Cell-Based Cancer Immunotherapy.

Cancer immunotherapies based on cytotoxic CD8 T lymphocytes (CTLs) are highly promising for cancer treatment. The specific interaction between T-cell receptors and peptide-MHC-I complexes (pMHC-I) on cancer cell membranes critically determines their therapeutic outcomes. However, the lack of appropriate endogenous antigens for MHC-I presentation disables tumor recognition by CTLs.

Supramolecular Nanofibers Ameliorate Bleomycin-Induced Pulmonary Fibrosis by Restoring Autophagy.

Idiopathic pulmonary fibrosis (IPF) is a progressive and ultimately fatal interstitial lung disease, with limited therapeutic options available. Impaired autophagy resulting from aberrant TRB3/p62 protein-protein interactions (PPIs) contributes to the progression of IPF. Restoration of autophagy by modulating the TRB3/p62 PPIs has rarely been reported for the treatment of IPF.

Burden of Stroke Attributable to Nonoptimal Temperature in 204 Countries and Territories: A Population-Based Study, 1990-2019.

Background And Objectives: Stroke attributable to nonoptimal temperature needs more attention with dramatic climate change. The aim of this study was to estimate the global burden and distribution characteristics of the burden.

Methods: In this ecological study, we collected data from the Climate Research Unit Gridded Time Series, the World Bank databases, and the Global Burden of Diseases study to estimate the distribution of burden.

Efficacy of additional locoregional therapy based on systemic therapy after intrahepatic progression for BCLC stage B/C hepatocellular carcinoma: A real-world study.

Background: The leading course of death in patients with advanced hepatocellular carcinoma (HCC) is intrahepatic progression and associated hepatic failure. The study aimed to evaluate the efficacy of locoregional therapy targeting intrahepatic lesions after intrahepatic progression for advanced HCC.

Methods: Consecutive 263 HCC patients who received lenvatinib combined with immunotherapy were reviewed.