Engineering a recombinant VP2-Based neutralizing epitope vaccine candidate against canine parvovirus: a preliminary immunogenicity assessment.

Background: Canine parvovirus (CPV) poses a severe threat to canine health, necessitating the development of safer and more effective vaccines. While traditional vaccines carry risks of virulence reversion and environmental contamination, subunit vaccines-especially neutralizing epitope vaccines-offer promising alternatives by eliciting targeted immune responses with enhanced safety.

Methods: We employed bacterial display technology to express 11 overlapping CPV VP2 gene fragments on the periplasmic membrane of E.

Bisphenol A and its analogs perturb primitive myelopoiesis and inhibit innate immune cell formation during early developmental stages of zebrafish.

Perinatal exposure to bisphenol A (BPA) promotes the development of inflammatory and immune diseases. Nevertheless, the potential mechanisms of this effect and the developmental immunotoxicity of BPA analogs are unclear. Here, embryonic zebrafish were exposed to a range of concentrations of BPA and its analogs.

The Relationship Between Cardiometabolic Index and New-Onset Diabetes in Adults Aged Over 45: A Longitudinal Analysis Based on CHARLS.

The cardiometabolic index (CMI) serves as a comprehensive indicator of metabolic risk. Recent findings indicate a potential association between CMI and the likelihood of diabetes onset. The objective of this research is to explore the correlation between initial CMI values and the occurrence of newly diagnosed diabetes mellitus in individuals aged 45 years and above in China.

Effect of ibuprofen on the behavior, histopathology, antioxidant system, DNA damage, and apoptosis of Asian freshwater clams (Corbicula fluminea).

The accumulation of ibuprofen (IBU) in freshwater sediments poses significant risks to benthic bivalves, yet its chronic ecological impacts remain insufficiently studied. Here, Asian clams (Corbicula fluminea) were exposed to 0, 20, 200, and 2000 µg/L IBU for 28 days. The viscera were identified as the primary target organ for IBU toxicity, with bioconcentration factors (BCFs) ranging from 3.

Predicting carotid atherosclerosis in latent autoimmune diabetes in adult patients using machine learning models: a retrospective study.

Background: Latent autoimmune diabetes in adults (LADA) is a slowly progressing form of diabetes with autoimmune origins. Patients with LADA are at an elevated risk of developing cardiovascular diseases, including carotid atherosclerosis. While machine learning models have been widely used in predicting cardiovascular risks in Type 1 and Type 2 diabetes, research on LADA remains limited.

Effective IHH gene knockout by CRISPR/Cas9 system in chicken DF-1 cells.

Indian hedgehog (IHH) gene codes an important signal molecule mediating chondrogenesis and bone development in chickens, which are key factors that affect body weight and several other significant economic traits. The aim of this study was to construct an IHH knockout cell model using CRISPR-associated protein 9 (CRISPR/Cas9) technology to further analyze the function of IHH. TA cloning was used to screen the single-guide RNA (sgRNA1) [45 %] and sgRNA3 (30.

Nanosecond Laser Synthesis of MXene-Derived TiO/High-Entropy Alloys for Photo-Assisted Zinc-Air Batteries.

Photo-assisted zinc-air batteries have garnered significant attention for applying solar energy to decrease the charge voltage and improve energy efficiency. However, the uniform and rapid synthesis of highly active, stable, and low-cost photoelectrocatalysts for zinc-air batteries remains a significant challenge. Herein, a pulsed laser method is reported for the rapid preparation of MXene-derived TiO/high-entropy alloy heterojunctions (M-TiO/HEAs) as photoelectrocatalysts.

Environmental levels of bis(2-ethylhexyl)-tetrabromophthalate (TBPH) cause thyroid toxicity and early carcinogenic signals in juvenile fish.

Exposure to novel brominated flame retardants (NBFRs) may engender thyroid dysfunction and even increase thyroid cancer risk. However, information regarding their thyroid toxic or disorder potential is primarily limited to thyroid hormone homeostasis. Here, we conducted a 28-day exposure experiment with juvenile rare minnows (Gobiocypris rarus) to 0.

FGF-21 fusion proteins ameliorate atopic dermatitis by inhibiting the TLR/TSLP signaling pathway: Anti-inflammatory and skin barrier repair effects.

Atopic dermatitis (AD) is a chronic inflammatory skin disorder with limited treatment options, often accompanied by adverse effects. Consequently, the development of novel therapeutics with enhanced efficacy and minimal side effects is of critical importance. This study explores the therapeutic potential and underlying mechanisms of three fibroblast growth factor 21 (FGF-21) fusion proteins (FGF-21-TAT, FGF-21-R11, and FGF-21-Fc-using a 2,4-dinitrochlorobenzene (DNCB)-induced AD mouse model.

Impact of exercise on health outcomes in people with cancer: an umbrella review of systematic reviews and meta-analyses of randomised controlled trials.

Objective: To examine the comprehensive health impacts of exercise on people with cancer by systematically summarising existing evidence and assessing the strength and reliability of the associations.

Design: Umbrella review of meta-analyses.

Data Source: PubMed, Embase, Cochrane and Web of Science databases were searched from their inception to 23 July 2024.

as a potential biomarker for brain metastasis from lung adenocarcinoma via cerebrospinal fluid liquid biopsy.

Background: Brain metastasis (BM) explains the majority of lung cancer-related mortality, especially lung adenocarcinoma (LUAD). The extensive clinical adoption of liquid biopsy presents a minimally invasive approach for the early detection and treatment management of brain metastasis from lung adenocarcinoma (BM-LUAD). Nonetheless, biomarkers for assessing BM-LUAD remain insufficient.

Association Between Circulating Vitamin C Concentrations and Risk of Diabetes Mellitus: Dual Evidence From NHANES Database and Mendelian Randomization Analysis.

Objectives: A relationship between vitamin C and diabetes risk has been proposed. Given the limited evidence specifically for type 1 diabetes (T1DM), we examined the association between vitamin C and T1DM using standard epidemiologic and Mendelian randomization (MR) approaches.

Methods: Data from a cross-sectional study using the National Health and Nutrition Examination Survey (NHANES; n=15,080 adults) were analyzed using logistic regression to estimate the association between vitamin C and diabetes risk, adjusting for demographic and lifestyle factors.

Mucosal-associated invariant T cells correlate with myocardial ischaemia and remodelling in coronary artery disease.

Objectives: Myocardial ischaemia and remodelling are major contributors to the progression and mortality of coronary artery disease (CAD). Previous studies have shown immune cell alterations in CAD patients, but their characteristics and associations with myocardial ischaemia and remodelling remain unclear.

Methods: We compared immune cell changes among patients without CAD, those with CAD and those with CAD and heart failure (HF).

A study on critical dislocation of gasket for segmental linings of shield tunnels.

Dislocation of segments in shield tunnels significantly contributes to joint leakage, making it crucial to identify the critical dislocation amount of segment linings. To explore the waterproofing mechanism of sealing gaskets under water pressure, a structural coupling finite element analysis model was created. This model simulates water intrusion dynamics at segment joints, analyzing contact stress distribution and waterproof performance across various dislocation amounts.

Synergistic effect of canine FGF-21 combined with insulin in the treatment of canine diabetes.

Previous studies have shown that FGF-21 can ameliorate hyperglycemia and improve the level of oxidative stress in vivo in diabetic mice. The hypoglycemic effect is safe and lasting, but it takes a longer time to exert its effect. Insulin treatment of canine diabetes takes effect quickly; however, its action time is short, and it is prone to cause hypoglycemia.

Fibroblast growth factor 21 alleviated atopic march by inhibiting the differentiation of type 2 helper T cells.

Background: The blood FGF21 expression has been previously suggested to increase in patients developing atopic dermatitis (AD) and asthma. However, its impact on atopic march is rarely analyzed. The present work focused on investigating the role of Fibroblast Growth Factor 21(FGF21) in atopic march mice and its underlying mechanisms.

Comparison of the clinical efficacy of lateral versus medial unicondylar replacement for unicompartmental osteoarthritis of the knee: a meta-analysis.

Objective: This meta-analysis evaluates the comparative efficacy of lateral unicompartmental arthroplasty (UKA) versus medial UKA in treating unicompartmental knee osteoarthritis (KOA).

Methods: We systematically searched Cochrane, PubMed, Embase, and Web of Science databases from January 2000 to September 2024. Literature screening, quality assessment, and data extraction were conducted based on predefined inclusion and exclusion criteria.

Comprehensive assessment of the safety of bisphenol A and its analogs based on multi-toxicity tests in vitro.

As substitutes for bisphenol A (BPA), bisphenol analogs (BPs) have raised concerns due to their frequent environmental detection and unclear safety. Here, the cytotoxicity, endocrine disruption, neurotoxicity, aryl hydrocarbon receptor (AhR) activity, and genotoxicity of nine BPs and BPA were evaluated in three types of cell lines. Over half of the tested BPs exhibited greater cytotoxicity than BPA, with IC50 values showing a linear correlation with Log (R²=0.

UV/Ozone-Induced Interface Engineering for High-Performance Horizontal Organic Light-Emitting Transistors Operating at Low Voltage.

Multifunctional organic light-emitting transistors (OLETs), which combine electric-switching and light-producing capabilities into a single device, are attracting increasing interest as promising candidates for new-generation display technology. Despite advancements in the design of organic luminescent materials and the optimization of device geometry configurations, maintaining operating voltage low while enhancing optical performances remains a key challenge in horizontally structured OLETs. Here, a simple and effective interfacial engineering strategy is employed to improve the optical properties of horizontal OLETs operating at low voltage, by introducing ultraviolet ozone (UVO)-induced surface modification on high-k dielectrics.

Persist or resist: Immune checkpoint inhibitors in EGFR-mutated NSCLC.

Although epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs), especially third-generation TKIs, have significantly improved the progression-free survival and overall survival of non-small cell lung cancer (NSCLC) patients with EGFR mutation, TKI resistance is inevitable for most patients. Over the past few years, immune checkpoint inhibitors (ICIs) have significantly improved the survival for EGFR-wild type NSCLC patients. However, no significantly improved benefits were observed with ICI monotherapy in EGFR-mutated patients.

Topologically-protected optical bistability based on two-dimensional photonic crystal L6 nanocavity dimer array.

We numerically investigate the optical bistability from a two-dimensional photonic crystal L6 nanocavity dimer array structure configured under the Su-Schrieffer-Heeger model. The localized electric field in the topological edge state is highly enhanced, which gives rise to strong nonlinear phenomena such as optical bistability. In comparison, a topologically trivial nanocavity is also designed and its field strength distribution and optical bistable response are also simulated.

The association between the triglyceride-glucose index and vitamin D status: a systematic review and meta-analysis.

Objective: This study aims to explore the association between the triglyceride-glucose (TyG) index and vitamin D status to enhance our understanding of how vitamin D status relates to metabolic health and to provide evidence for the early diagnosis of vitamin D deficiency (VDD) using the TyG index.

Methods: We conducted a comprehensive search in various databases, including PubMed, Web of Science, Embase, Cochrane Library, China National Knowledge Infrastructure (CNKI), China Biology Medicine disc, China Science and Technology Journal Database, and Wanfang Data to gather articles published from the inception of these databases until February 19, 2024. We assessed the quality of included studies using the Newcastle-Ottawa Scale (NOS) for case-control studies and the Agency for Healthcare Research and Quality (AHRQ) methodology checklist for cross-sectional studies.

Mechanical memory based biofabrication of hierarchical elastic cardiac tissue.

Mimicking the multilayered, anisotropic, elastic structure of cardiac tissues for controlled guidiance of 3D cellular orientation is essential in designing bionic scaffolds for cardiac tissue biofabrication. Here, a hierarchically organized, anisotropic, wavy and conductive polycaprolactone/Au scaffold was created in a facile fashion based on mechanical memory during fabrication. The bionic 3D scaffold shows good biocompatibility, excellent biomimetic mechanical properties that guide myoblast alignment, support the hyperelastic behavior observed in native cardiac muscle tissue, and promote myotube maturation, which holds potential for cardiac muscle engineering and the establishment of anculture platform for drug screening.