Evaluating the performance of four anti-dsDNA antibody detection methods.

ObjectiveTo evaluate the concordance of four anti-dsDNA antibody detection methods-Crithidia luciliae indirect immunofluorescence test (CLIFT), enzyme-linked immunosorbent assay (ELISA), acridine ester direct chemiluminescence immunoassay (CLIA), and digital liquid chip method (DLCM)-and to assess their diagnostic efficacy in systemic lupus erythematosus (SLE) patients.MethodsA total of 285 serum samples were collected, including 170 SLE patients, 39 with non-SLE autoimmune diseases (AIDs), 28 with non-AIDs, and 48 undiagnosed cases. The concordance and diagnostic performance of anti-dsDNA antibody methods were analyzed.

Association Between Health-Related Quality of Life and Willingness of COVID-19 Vaccination: A Cross-sectional Study in China.

Objectives: This study aims to explore the association between Health-Related Quality of Life (HRQoL) and people's willingness to receive the coronavirus disease 2019 (COVID-19) vaccination.

Methods: This survey was conducted in November 2020. 1461 participants (convenient sampling method) completed the online questionnaire.

Risk factors of immune checkpoint inhibitor-related cardiotoxicity: a scoping review.

Background: Cardiotoxicity due to immune checkpoint inhibitors (ICIs) is not a common phenomenon but can lead to fatal outcomes in cancer patients. The clinical manifestations of this cardiotoxicity are also nonspecific and difficult to identify promptly. Hence, the study aimed to define the risk factors of ICI-related cardiotoxicity to enable early identification of high-risk groups and implement timely intervention for patients receiving ICI therapy.

Development and validation of a risk prediction model for hypoproteinemia after adult cardiac valve surgery: implications for clinical care.

Objective: To construct and validate a risk prediction model for hypoproteinemia in adults following cardiac valve surgery with cardiopulmonary bypass (CPB), providing medical staff with an effective tool for early identification and intervention.

Methods: This retrospective cohort study analyzed clinical data from 259 patients who underwent CPB-assisted heart valve surgery at the Department of Cardiovascular Surgery, First Affiliated Hospital of China University of Science and Technology, between January and December 2023. Patients were divided into two groups based on whether their serum albumin levels fell below 35 g/L within 48 hours postoperatively: the hypoproteinemia group ( = 144) and the non-hypoproteinemia group ( = 115).

Self-Iron-Enriched Bacterial Membrane Nanovesicles for Cascade and Multi-Modal Antitumor Therapy.

The integration of microbiology and nanotechnology offers a novel strategy for cancer treatment. In this study, we innovatively propose the use of bacterial membranes as nanocarriers. These membranes possess a simple and unique self-enriching property for iron, which, in addition to the inherent immune effects of the membrane itself, can facilitate tumor chemodynamic therapy through Fenton reactions.

Association of the time interval between first and last birth with obesity in middle-aged postmenopausal Chinese women: a cross-sectional study in southern China.

Background: Birth interval is acknowledged as a significant factor affecting the health of women and their children. This study aimed to investigate the relationship between the time interval from the first to the last birth and the prevalence of general obesity, abdominal obesity, or both, among middle-aged postmenopausal Chinese women.

Methods: This cross-sectional survey examined 4,799 Chinese postmenopausal women, aged 45-59 years, who had at least one live birth.

polysaccharide alleviates dextran sulfate sodium-induced ulcerative colitis in mice by repairing the intestinal barrier and regulating the intestinal microecology.

Polysaccharides, vital biological macromolecules ubiquitous in organisms, have garnered attention as potential therapeutic candidates for ulcerative colitis (UC). However, the therapeutic potential of polysaccharides (RCP) in UC remains largely unexplored. The RCP was prepared by boiling water extraction, 80% alcohol precipitation, membrane separation, and D101 macroporous resin purification.

The impact of a PERMA model-based positive psychology intervention on fear of stroke recurrence: a randomized controlled trial.

Introduction: This study aimed to examine the effects of a positive psychological intervention, grounded in the PERMA model, on fear levels, psychological capital, overall well-being, and quality of life among stroke patients.

Methods: A single-blind, two-arm randomized controlled trial with a repeated measures design was conducted at the Affiliated Hospital of Jiangnan University from January to December 2023. A total of 125 patients experiencing fear of stroke recurrence were randomly assigned to either the intervention group ( = 63), which received a positive psychological intervention based on the PERMA model, or the control group ( = 62), which received standard care.

Risk factors associated with immune-related severe adverse events in patients with cancer: A scoping review.

Objective: Increasing reports of immune-related severe adverse events (ir-SAEs) among patients with cancer treated with immune checkpoint inhibitors (ICIs) have raised significant concerns regarding patient safety. Although considerable research has addressed immune-related adverse events (irAEs), the risk factors specifically associated with ir-SAEs remain less well defined. This study aims to identify and synthesize the risk factors contributing to ir-SAEs in patients with cancer.

Harnessing Membrane Coating for Probiotic Therapy in Inflammatory Bowel Disease.

Inflammatory bowel diseases (IBDs) are characterized by dysregulated inflammatory responses, increased reactive oxygen species (ROS), and compromised intestinal barrier function. Current clinical treatments often yield suboptimal responses and significant side effects. Given the great potential of (AKK) bacterial membrane components in IBD treatment and the possible pathogenicity of live bacteria, to essentially address these challenges, we develop a natural membrane-based single-cell coating, incorporating a self-assembled AKK bacterial membrane (AKM) and astaxanthin (AST), forming a protective biological barrier on probiotics.

Prevalence and Related Factors of Hypokalemia in Patients with Acute Ischemic Stroke.

Aim: This study aimed to investigate the prevalence and associated factors of hypokalemia in patients with acute ischemic stroke.

Methods: A cohort of 996 patients was assessed using a general data questionnaire, laboratory indicators, the NIH Stroke Scale (NIHSS), the Barthel Index (BI), the Frail scale, Nutritional Risk Screening (NRS-2002), and the Kubota drinking water test.

Results: Among the 996 patients, 205 (20.

Factors associated with immune‑related severe adverse events (Review).

Immune checkpoint inhibitors (ICIs) are frequently used in cancer treatment. Despite their clinical benefits, they can also cause a wide range of immune-related adverse events (ir-AEs). The overall incidence of irAEs in cancer patients treated with immunotherapy ranges from 70-90%, while that of immune-related severe adverse events (ir-SAEs) is 10-43%.

Effects of multicomponent exercise nursing intervention in elderly stroke patients with frailty: a randomized controlled trial.

This study examines how multicomponent exercise nursing interventions affect the state of frailty, daily activities, and quality of life in elderly stroke patients with frailty. A total of 125 elderly stroke patients with frailty were randomly assigned to either a control group ( = 62) or an intervention group ( = 63). The control group received standard nursing care, while the intervention group received a multicomponent exercise nursing intervention in addition to standard care.

Human TCR repertoire in cancer.

Background: T cells, the "superstar" of the immune system, play a crucial role in antitumor immunity. T-cell receptors (TCR) are crucial molecules that enable T cells to identify antigens and start immunological responses. The body has evolved a unique method for rearrangement, resulting in a vast diversity of TCR repertoires.

Clinical Performance of the Line Immunoassay and Digital Liquid Chip Method for Detecting Autoimmune Liver Disease Autoantibodies.

Context.—: The identification of autoantibodies associated with autoimmune liver disease (ALD) is crucial for diagnosis and management. Various laboratory methods have been introduced to detect autoantibody profiles.

The impact of WeChat online education and care on the mental distress of caregivers and satisfaction of elderly postoperative colorectal cancer patients.

Objective: WeChat-based nursing interventions alleviate mental distress. This study intended to investigate the effect of WeChat online education and care (WOEC) on the mental health of caregivers and the satisfaction of elderly postoperative colorectal cancer (CRC) patients.

Methods: In total, 92 elderly postoperative CRC patients and 92 caregivers were randomly separated into the WOEC group (46 patients and 46 caregivers) and the control care group (46 patients and 46 caregivers).

Clinical Performance of the Line Immunoassay, Digital Liquid Chip Method, and Chemiluminescent Immunoassay for Detecting Specific Antinuclear Antibodies.

Context: Antinuclear antibodies (ANAs) against certain antigens are useful for identifying autoimmune disorders. Although new solid phase-based immunoassays have been developed for evaluating ANAs, the conventional line immunoassay (LIA) is commonly used in clinical practice.

Objective: To compare the clinical performance of 2 newly developed methods for detecting specific ANAs with LIA.

Clinical Significance of Serum IgG4 in the Diagnosis and Treatment Response of IgG4-Related Disease in Adults of Southwest China: A Retrospective Study.

Background: There is no standard cut-off value of serum IgG4 concentration and serum IgG4/total IgG ratio for the diagnosis of IgG4-related disease (IgG4-RD) or as a marker of treatment responses. We aimed to explore this issue through a retrospective cohort analysis of adults in southwest China.

Methods: The diagnostic performance of serum IgG4 concentration and IgG4/IgG ratio for IgG4-RD was evaluated in a retrospective analysis of 177 adults newly diagnosed as having IgG4-RD and 877 adults without IgG4-RD.

Mycobiome Study Reveals Different Pathogens of Vulvovaginal Candidiasis Shape Characteristic Vaginal Bacteriome.

Vulvovaginal candidiasis (VVC) can alter the vaginal microbiome composition and structure, and this may be correlated with its variable treatment efficacy. Integrated analysis of the mycobiome and bacteriome in VVC could facilitate accurate diagnosis of infected patients and further decipher the characterized bacteriome in different types of VVC. Our mycobiome analysis determined two common types of VVC, which were clustered into two community state types (CSTs) featured by Candida glabrata (CST I) and Candida albicans (CST II).

Association of Gene Polymorphisms in CXC Chemokine Receptor 5 with Rheumatoid Arthritis Susceptibility.

Rheumatoid arthritis (RA) is caused by complicated interactions between genes and the environment. CXC chemokine receptor 5 (CXCR5) is required for B and T follicular helper cell migration and humoral immunity generation. Therefore, this study aimed to assess whether polymorphisms of the CXCR5 gene are implicated in RA development and progression.

The relationship between anthropometric indicators and health-related quality of life in a community-based adult population: A cross-sectional study in Southern China.

Background: This study was designed to analyze the relationship of waist circumference (WC), body mass index (BMI), waist-to-hip ratio (WHR), waist-to-height ratio (WHtR), relative fat mass (RFM), lipid accumulation product (LAP) and health-related quality of life (HRQoL) in the community-dwelling population of southern China and to explore the independent contribution of socio-demographic characteristics, number of chronic diseases and anthropometric indicators to HRQoL in that population.

Methods: This community-based cross-sectional survey studied 2,663 adults aged 18 years and older. HRQoL was assessed by the 3-level EuroQol 5-dimensional scale (EQ-5D-3L), and HRQoL were calculated using the Chinese EQ-5D-3L value set.

Circulating IL-1, IL-17, and IP-10 as Potential Predictors of Hepatitis B Virus Infection Prognosis.

Circulating cytokines and chemokines play critical roles in hepatitis B virus (HBV) infection. Here, we explored the effects of proinflammatory and anti-inflammatory effector molecules on HBV progression, e antigen seroconversion, and liver function. Our results showed that circulating interleukin (IL)-17 may be helpful in HBV spontaneous clearance [odds ratio (OR) = 1.

Illiciumlignans G-O from the leaves of and their anti-inflammatory activities.

Phytochemical investigations on the dry leaves of have led to the isolation of 24 lignans. Illiciumlignans G-K (1-5) were five undescribed benzofuran lignans, illiciumlignan L (6) was one undescribed ditetrahydrofuran lignan, illiciumlignans M-O (7-9) were three new sesquilignans, and compounds 10, 12, 13, 15, and 18-21 were firstly isolated from the genus . Their structures were elucidated by detailed spectroscopic analyses (UV, IR, HR-ESI-MS, and NMR) and CD experiments.

Detection of IgA Antiphospholipid Antibodies Does not Improve Thrombotic Antiphospholipid Syndrome Classification: A two-Center Study.

Background: Thrombotic antiphospholipid syndrome (APS) is a systemic autoimmune disease; its diagnosis requires meeting both clinical and laboratory criteria. Prevalence rates of immunoglobulin (Ig) A anticardiolipin antibodies (aCL) and IgA anti-β glycoprotein I antibodies (aβGPI) remain unknown, and the clinical value of these antibodies to APS classification remains controversial. Therefore, we aimed to examine both items in the Chinese population.