Simultaneous EGFR mutation detection using a sensitive dual-target biosensor based on 3D hollow carbon nanosphere synergy with circular bipedal DNA walkers.

A sensitive dual-target electrochemical biosensor was developed integrating circular bipedal DNA walkers with a three-dimensional hollow carbon nanospheres-tetrametallic nanoparticle (3D HCNs-TT NPs) modified platform, enabling concurrent detection of two critical epidermal growth factor receptor (EGFR) exon 19 mutations: E746_A750 deletion (Mut DNA A) and L747_S752delinsS (Mut DNA B). The circular bipedal DNA walkers address the limitations of low amplification efficiency in traditional single-legged DNA walkers and mitigate the sensitivity reduction caused by excessively long walking strands. Additionally, we used the 3D HCNs-TT NPs nanocomposite materials to modify the electrodes, which significantly increased the electron transfer rate and provided more active sites for molecular probe loading, thereby further enhancing the performance of the detection platform.

SLC10A3 regulates ferroptosis of glioblastoma through the STAT3/GPX4 pathway.

Ferroptosis is closely related to disease progression and treatment response in human brain gliomas; however, the regulatory mechanisms involved remain to be elucidated. Identifying new ferroptosis regulatory factors holds promise for addressing the aberrant regulation of ferroptosis in glioblastoma (GBM) and overcoming its treatment resistance. In this research, bioinformatics revealed that SLC10A3 is upregulated in GBM and correlates with poor patient prognosis.

Ultrasensitive photoelectrochemical biosensor of amyloid-β oligomer detection via porphyrin-based covalent organic frameworks enhanced by iron single-atom catalysts.

Accurate and sensitive detection of amyloid-β oligomer (AβO) is essential for understanding the development and progression of Alzheimer's disease (AD). Herein, an ultrasensitive photoelectrochemical (PEC) biosensing platform was developed by integrating photoelectrically active porphyrin-based covalent organic frameworks with Fe single-atom catalysts (Fe SACs) to enhance PEC signal. The Fe SACs demonstrate remarkable peroxidase-like activity, enabling them to oxidize 4-chloro-1-naphthol (4-CN) to generate insoluble 4-chloro-1-naphthol dimer (4-CD) precipitation on the photoelectrode surface.

The impact of microplastics on the adsorption of 2,4,6-tribromophenol in soils: Competitive adsorption.

Soil is a major sink for microplastics (MPs) which can interact with organic contaminants affecting their transport behavior. The impact of MPs in soil matrix is complicated depending on soil-MPs, soil-contaminants and MPs-contaminants interactions, however the study on their interactions is limited. In this study, polyethylene (PE) was selected to investigate its effects on 2,4,6-tribromophenol (TBP) adsorption in soils.

The Strategies Microalgae Adopt to Counteract the Toxic Effect of Heavy Metals.

Besides biomass production, some microalgae have been used to treat wastewater contamination. However, in general, high concentrations of heavy metals significantly inhibit algal growth. We thus need to find ways to promote the resistance of microalgae to heavy metals, increase their growth rate under stress, and achieve coupling of heavy metal removal and biomass production simultaneously.

Combined Analysis of Transcriptomes and Metabolomes Reveals That MeJA-Mediated Flavonoid Biosynthesis Is Crucial for Pigment Deposition in Naturally Colored Green Cotton Fibers.

: Green cotton fibers (GCFs) are valued for their natural coloration and eco-friendly properties, but their pigmentation mechanisms remain unclear, limiting their wider application in the textile industry. This study aims to uncover the key regulatory genes and metabolic pathways involved in GCF coloration. : We conducted transcriptome and metabolome profiling of green and white cotton fibers at different developmental stages to identify differences in gene expression and metabolite accumulation related to pigmentation.

Design of a nonlinear metastructure for temperature detection and biosensing based on the second harmonic generation in theory.

When the second harmonic generation (SHG) occurs in temperature-controlled ferroelectric crystals, it serves as an effective method for detecting temperature and changes in the refractive index (RI). A one-dimensional nonlinear metastructure (NM) composed of periodically poled lithium niobate crystals is designed precisely to exhibit significant SHG enhancement in the visible to near-infrared spectral range. When a tunable fundamental electromagnetic wave is incident at a frequency () near 241.

Socioeconomic determinants of the leprosy risk in Yunnan Province, China: a county-level spatiotemporal study.

Background: Making leprosy history in low-burden countries is a crucial step in achieving the World Health Organization's 2021-2030 Global Leprosy Strategy. Since leprosy has been contained at the national level in these countries, current efforts to eliminate leprosy have focused on specific leprosy high-risk areas. Quantifying the factors associated with leprosy risk would assist in identifying high-risk areas and the required efforts for leprosy elimination, which are still inadequate in these countries.

Analysis of growing season drought characteristics and driving factors for vegetation in the Santun River Irrigation Area in Xinjiang.

Global warming is exacerbating the occurrence of droughts, which have a significant impact on society. Drought is one of the main factors limiting the development of the Santun River Irrigation Area in Xinjiang. Clarifying the driving mechanism and spatial and temporal evolution characteristics of drought in this irrigation area is crucial for ensuring the sustainable development of agriculture.

Nerve injury induces pain hypersensitivity and anxiety-related behaviours and is associated with amygdala activation in male mice.

Background: Neuropathic pain is a common health problem, often accompanied by anxiety. The amygdala plays a crucial role in emotional processing and the basolateral amygdala (BLA) and the central amygdala (CeA) are primary components of the amygdala. The c-Fos is considered indicative of neuronal activation.

NSMCE2 promotes the occurrence and development of HCC by regulating the SUMOylation of PPARα.

Background: Primary liver cancer is a malignant tumor of the digestive system and ranks as the sixth most commonly diagnosed cancer globally. It has also risen to become the third leading cause of cancer-related deaths globally, following lung and colorectal cancers. Hepatocellular carcinoma (HCC) accounts for the majority of primary liver cancer, approximately 75 to 85 %.

Extraction, purification, structural characterization, bioactivities and application of polysaccharides from different parts of pine.

The pine plant belongs to the genus Pinus and the Pinaceae family. Approximately 100 species are predominantly distributed in the northern hemisphere. Historically, various parts of pine trees, including pollen, needles, cones, and nuts, have been used in traditional medicine for their therapeutic properties.

Fabrication and Performance of Aluminum-Based Composite Wicks Using a Two-Step Laser-Sintering Process.

The evolution of 5G technology necessitates effective thermal management strategies for compact, high-power devices. The potential of aluminum-based vapor chambers (VCs) as thermal management solutions is recognized, yet the heat transfer performance is limited by the capillary constraints of the wick structures. This study proposes a laser-sintered composite wick to address this limitation.

Catalytic Pyrolysis of Biomass: A Review of Zeolite, Carbonaceous, and Metal Oxide Catalysts.

This review provides an exploration of various catalytic pyrolysis techniques for bio-oil production, focusing on the effects of different pyrolysis methods (slow, fast, and flash pyrolysis) on bio-oil yield and composition. The review also discusses key factors influencing bio-oil production, including feedstock composition (cellulose, hemicellulose, and lignin), and the role of catalytic materials in enhancing yield and product selectivity. Three primary classes of catalysts-zeolites, carbonaceous materials, and metal oxides-are thoroughly examined, with a discussion on the differences between bulk catalysts and nanocatalysts.

Autocatalytic, Brain Tumor-Targeting Delivery of Bardoxolone Methyl Self-Assembled Nanoparticles for Glioblastoma Treatment.

Glioblastoma multiforme (GBM) is a formidable cancer to treat due to the lack of effective drugs that can also efficiently cross the blood-brain barrier (BBB). Herein, a novel strategy involving the synthesis of p28 peptide-conjugated, lexiscan (LEX)-loaded, bardoxolone methyl (BM) self-assembled nanoparticles, designated as p28-LBM NPs, is introduced. These NPs are designed to overcome the dual challenges of effectively killing GBM cells and efficiently penetrating the brain.

Research progress on ferroptosis in Myelodysplastic syndromes.

Myelodysplastic syndromes (MDS) are a group of malignancies characterized by clonal proliferation of hematopoietic stem cells, ineffective hematopoiesis, peripheral cytopenias, and a high risk of transformation to acute myeloid leukemia. Current therapeutic strategies for MDS have limited efficacy. Thus, identifying new therapeutic targets and prognostic biomarkers is a critical future research direction.

Review of Biomass-Derived Carbon Nanomaterials-From 0D to 3D-For Supercapacitor Applications.

The transition to sustainable energy storage solutions has driven significant interest in supercapacitors, which offer high power density, rapid charge-discharge capabilities, and exceptional cycle stability. Biomass-derived carbon nanomaterials have emerged as compelling candidates for supercapacitor electrodes due to their renewable origins, environmental compatibility, and cost-effectiveness. This study explores recent advancements in tailoring structural properties, for example in preparation methods and activation, which are essential for efficient charge storage and rapid ion transport.

A three-site recognition cytosensor for accurate detection of circulating tumor cells based on novel branched Pt Au nanospheres and MnO-GO-Au nanosheets.

A novel electrochemical cytosensor was developed with a three-protein recognition strategy, employing branched Pt Au nanospheres (B-Pt-Au NPs) as tags and MnO-GO-Au nanosheet-modified electrodes for circulating tumor cells (CTC) detection. This system integrates specific magnetic separation and CTC enrichment with sensitive electrochemical detection to identify rare A549 cells in whole blood. MnO-GO-Au nanosheets, known for their high conductivity and biocompatibility, were used to modify a bare electrode, improving the electrochemical interface for biomolecules.

N-acetylcysteine remodels the tumor microenvironment of primary and recurrent mouse glioblastoma.

Purpose: Glioblastoma (GBM) exhibits a high ROS character, giving rise to an immunosuppressive microenvironment and tumor vascular abnormality. This study investigated the potential effect of N-acetylcysteine (NAC), an antioxidant, on primary and recurrent mouse brain tumors.

Methods: We measured reactive oxygen species (ROS)/ glutathione (GSH) levels in human GBM.

Simultaneous Determination and Pharmacokinetics of Three Steroidal Alkaloids in Rat Plasma by UHPLC-MS After Oral Administration of Three Different Preparations of Fritillaria ussuriensis Maxim Bulb.

Pharmacokinetic studies can provide a method for optimizing preparations. In this study, three most abundant compounds from Fritillaria ussuriensis bulb were isolated, and a selective, sensitive new analytical method was developed. This method aims for the simultaneous determination of peimisine-3-O-β-D-glucopyranoside (1), karelinine (2), and peimisine (3) in rat plasma after oral administration of F.

Depressive intensity, duration, and their associations with cognitive decline: a population-based study in Korea.

Existing epidemiological studies have ignored the effect of depressive duration on cognitive decline despite the presence of biological cues and understudied the depression-cognition association in Asian countries in the context of increasing cognitive burden worldwide. We aimed to comprehensively characterize the effects of depressive duration and intensity on cognitive decline at the population level. A total of 6406 individuals from the Korean Longitudinal Study of Aging (KLoSA) from 2010 to 2018 were included to generate four datasets with durations of 2, 4, 6, and 8 years.

Xanthones explore the mechanism of p53/p21 signaling pathway to prevent cardiac aging and epigenetic regulation of Nrf2 gene.

Gentianella acuta (GA) is a folk medicine used by Ewenki people in Inner Mongolia to treat heart disease. Transcriptional inhibition caused by the increase of DNMT1/3A/3B levels inhibited Nrf2, an anti-aging factor with antioxidant effect in aging myocardia, and the level of Nrf2 decreased with the increase of age. The main chemical component of GA, xanthones, can reverse this inhibition.

Association Between Multimorbidity and Depression in Older Adults: Evidence From Six Large Longitudinal Cohorts.

Background: Multimorbidity may increase the risk of depression in older adults, but the global average effect of multimorbidity on depression remains unknown. This research aimed to exclude the regional heterogeneity to quantify the exposure-response association between multimorbidity and depression in older adults worldwide.

Method: We collected 23,947 participants aged 65 years or above from six large prospective cohorts from developed and developing countries, including Mexico, South Korea, Europe, America, China, and England.