Repurposing drugs for the human dopamine transporter through WHALES descriptors-based virtual screening and bioactivity evaluation.

Computational approaches, encompassing both physics-based and machine learning (ML) methodologies, have gained substantial traction in drug repurposing efforts targeting specific therapeutic entities. The human dopamine (DA) transporter (hDAT) is the primary therapeutic target of numerous psychiatric medications. However, traditional hDAT-targeting drugs, which interact with the primary binding site, encounter significant limitations, including addictive potential and stimulant effects.

Timely Initiation of Argatroban Improves Prognosis in Patients With Acute Ischemic Stroke Attributed to Large Artery Atherosclerosis: A Cohort Study.

Introduction: Previous studies have demonstrated the benefits of anticoagulant therapy in acute ischemic stroke (AIS) of large artery atherosclerosis (LAA) etiology, although no prior research has examined the impact of the timing of anticoagulation initiation in this population.

Methods: A retrospective cohort study was conducted to assess the effect of early argatroban administration on clinical outcomes in AIS with LAA. Patients were stratified into an early administration group and a late administration group based on the time from stroke onset to argatroban initiation: ≤24 h and >24 h.

A case of primary papillary epithelial tumor of the sella with reverse polarity and paired box 8 expression.

Primary papillary epithelial tumor of the sella (PPETS) is a rare sellar neoplasm characterized by distinctive papillary architecture and thyroid transcription factor 1 (TTF-1) expression. DNA methylation profiling suggests its classification within the posterior pituitary tumor category. Here, we report a case of PPETS in a 37 year-old female presenting with amenorrhea, featuring unique morphological and immunohistochemical characteristics.

NAD-dependent Sirt6 is a key regulator involved in telomere shortening of in vitro-cultured preimplantation embryos.

Telomere length (TL) is important for maintaining the individual health of a species. Recent studies shows that in vitro fertilization therapy can drastically reduce TL in offspring, however, the underlying molecular mechanism remains unknown. Sirt6 is a NAD-dependent epigenetic regulator that has recently been found to play an important role in maintaining telomere stability.

Neoadjuvant chemoradiation with camrelizumab and nimotuzumab for initially inoperable esophageal squamous cell carcinoma: A single-arm phase 2 trial.

Background: Neoadjuvant regimen with a high surgical conversion rate was warranted for initially inoperable patients with locally advanced esophageal squamous cell carcinoma (LAESCC). To evaluate the efficacy and safety of neoadjuvant chemoradiotherapy combined with camrelizumab and nimotuzumab (NCRCN) followed by surgery in initially inoperable patients with LAESCC.

Methods: This cohort study was a prospective, one-arm, phase 2 study from December 2021 to December 2023.

Treatment of an invasive thymoma-like left innominate venous aneurysm using allogeneic pericardium with continuous horizontal mattress sutures: a case report.

Background: Left innominate vein aneurysm (LIVA) is a rare condition with an unclear cause. Only a few cases of this entity have been reported, and standard therapeutic guidelines for its diagnosis and treatment have not yet been established. There is no consensus about the optimal surgical access, thoracotomy may be the preferred method of treatment.

A nomogram integrating M2 macrophages and extracellular matrix components outperforms TNM classification in pulmonary sarcomatoid carcinoma prognosis: A two-center retrospective study.

Objective: Tumor microenvironment composition significantly influences tumor progression. This study aimed to explore the distribution of M2 tumor-associated macrophages (TAMs), reticular fibers (RFs), and collagen fibers (CFs) within the tumor microenvironment of pulmonary sarcomatoid carcinoma (PSC) and assess their clinicopathological significance.

Methods: Formalin-fixed paraffin tissue sections of 127 PSC patients from two medical centers were collected and analyzed by immunohistochemistry and the Gomori method.

Risk Factors and Construction of a Prediction Model for Early Postoperative Infections in Patients Who Underwent Kidney Transplantation.

Objective: This study explored infection risk factors within one month post-kidney transplantation (KT) and developed a clinical prediction model.

Methods: We retrospectively analyzed clinical data from KT patients treated at our hospital (January 2015-December 2024). Patients were categorized into infection or control groups based on 1-month postoperative infection status.

A single mutation may contribute to accelerated evolution of SARS-CoV-2 toward Omicron.

How SARS-CoV-2 Omicron evolved remains obscure. T492I, an Omicron-specific mutation encountered in SARS-CoV-2 nonstructural protein 4 (NSP4), enhances viral replication and alters nonstructural protein cleavage, inferring potentials to drive evolution. Through evolve-and-resequence experiments of SARS-CoV-2 wild-type (hCoV-19/USA/WA-CDC-02982585-001/2020, A) and Delta strains (B.

Quantum dot immunochromatographic strip for rapid and sensitive detection of H5 subtype avian influenza virus.

The H5 subtype avian influenza viruses (AIVs) pose a major threat to wild fowl and poultry. Additionally, they can overcome the species barrier, inducing human infection, which may become fatal. Thus, the H5 subtype AIVs remain a global public health burden, with a huge pandemic potential.

Risk factors for postoperative outcomes in laparoscopic pancreaticoduodenectomy: a retrospective cohort study from 2015 to 2023.

Background: Laparoscopic pancreaticoduodenectomy (LPD) is being increasingly performed for pancreatic tumors, offering advantages such as reduced recovery time and trauma. However, currently there is a knowledge gap in risk factors of postoperative complications, intensive care unit (ICU) admission, and prolonged hospital stays. Identifying for these outcomes is essential for improving patient management and surgical results.

C3a-C3aR1-mediated interactions between fibroblast-like synoviocytes and macrophages promote the progression of rheumatoid arthritis.

The inflammatory microenvironment in rheumatoid arthritis (RA) synovium is highly complex, comprising functional units known as cellular neighborhoods (CNs). However, how cell interactions within CNs shape the inflammatory microenvironment in RA remains unclear. Here, we utilized imaging mass cytometry (IMC) and single-cell RNA sequencing to dissect the CNs within RA synovium, uncovering critical cell-cell interactions and evaluating whether disrupting interaction signals could alleviate disease severity in collagen-induced arthritis (CIA) mouse model.

Targeting alveolar macrophages in tuberculosis: Exploiting trained immunity for novel therapeutic approaches.

Tuberculosis (TB) is an airborne infectious disease caused by the Mycobacterium tuberculosis (Mtb) complex organism. Alveolar macrophages (AMs) play key roles in immune defense, antigen presentation, immune regulation, and immune secretion during Mtb infection. Notably, AMs exhibit context-dependent dual functions: protective and pathogenic.

Clinicopathological Characteristics and Immune Microenvironment of Posterior Pituitary Tumours.

Aims: Posterior pituitary tumours (PPTs) represent a distinct group of low-grade neoplasms characterised by unique pathological features and immunohistochemical phenotypes. However, their precise immunological environment remains poorly understood, posing challenges to a deeper understanding of their biological behaviour and potential treatment implications. This study aimed to characterise the clinicopathological features and immune landscape of PPTs, focusing on the infiltration patterns of T cells and macrophages.

FRMD8 inhibits tumor metastasis in BRCA1-associated TNBC by negatively regulating tmTNF-α.

Triple-negative breast cancer (TNBC), particularly in patients with metastasis, is associated with limited treatment options and shorter survival times. In this study, through library screening and animal experiments, we identified that the low expression of FERM domain-containing protein 8 (FRMD8) in breast cancer type 1 susceptibility protein (BRCA1)-mutant breast cancer cells (TNBC subtype), significantly enhances the metastatic potential of tumor cells to various organs. Further functional experiments revealed that FRMD8 inhibited the cleavage of tmTNF-α (transmembrane TNF-α) and promoted the expression of surface tmTNF-α.

Portable lab-on-a-chip platform enabling multiplex recombinant enzyme polymerase amplification detection of H5/H7/H10 avian influenza virus subtypes.

The zoonotic nature of influenza pathogens creates substantial health security risks, jeopardizing the welfare of interconnected human and animal ecosystems. The H5/H7/H10 avian influenza virus (AIV) variants demonstrate persistent endemicity in poultry reservoirs and recurrent zoonotic jumps precipitating fatal human infections. Therefore, the innovation of multiplex diagnostic platforms integrating expedited processing, enhanced sensitivity, and subtype-specific discrimination has emerged as a pivotal strategy to curb epidemiological escalation.

Endoscopic Transoropharyngeal Retropharyngeal Lymph Node (RPLN) Dissection for RPLN Recurrence Nasopharyngeal Carcinoma.

Background: Retropharyngeal lymph node (RPLN) dissection is difficult and lacks an ideal approach. We aimed to examine the efficacy of endoscopic transoropharyngeal RPLN dissection for patients with nasopharyngeal carcinoma (NPC) with RPLN recurrence.

Patients And Methods: From January 2022 to July 2023, 25 patients with RPLN recurrence NPC were enrolled in the study.

Superior diagnostic performance of Grocott methenamine silver staining in pulmonary cryptococcosis: a multicenter, large-sample cohort study.

Introduction: In the histopathological diagnosis of pulmonary fungal infections, particularly pulmonary cryptococcosis, the diagnostic performance of different staining techniques varies significantly, often confounding pathologists. This study aims to systematically analyze Grocott methenamine silver (GMS), periodic acid-Schiff (PAS), and alcian blue (AB) staining methods to establish evidence-based diagnostic criteria.

Methods: We incorporated histopathological data from 790 cases of pulmonary cryptococcosis that were definitively diagnosed in six tertiary hospitals.

FAM50A drives breast cancer brain metastasis through interaction with C9ORF78 to enhance ʟ-asparagine production.

Elevated levels of asparagine, catalyzed by asparagine synthetase (ASNS), have been identified as a prerequisite for lung metastasis in breast cancer. However, the roles and regulatory mechanisms of ASNS in breast cancer brain metastasis (BCBM) are not well understood. Our study revealed that the family with sequence similarity 50 member A (FAM50A) gene substantially modulates the brain metastatic potential of breast cancer by up-regulating ASNS and promoting asparagine biosynthesis.

WRINKLED1 transcription factor activates lipid biosynthesis to enhance oil accumulation in Pinus koraiensis.

Pinus koraiensis is an economically important oil-producing conifer whose seeds are enriched in unsaturated fatty acids, exhibiting significant nutritional and industrial value. However, the molecular mechanisms underlying lipid biosynthesis in P. koraiensis remain largely unexplored, particularly regarding the role of transcription factors in seed oil accumulation.

Effects of varied rTMS frequencies on cognitive function in individuals with chronic schizophrenia: A double-blind randomized controlled trial.

Background: Accumulating evidence has indicated the effects of repetitive transcranial magnetic stimulation (rTMS) on cognitive improvements in individuals with schizophrenia. However, the comparative effectiveness of low-frequency rTMS versus high-frequency rTMS on cognitive improvements remains unclear. This study aims to investigate the difference in the efficacy of low and high-frequency rTMS on cognitive function in chronic individuals with schizophrenia.

Traditional Chinese medicine Jianpi Fuzheng formula for vascular event recurrence rates and clinical prognosis in ischemic stroke: A randomized, double-blind, placebo-controlled trial.

Ethnopharmacological Relevance: Jianpi Fuzheng formula (JPFZF) is a traditional Chinese medicine formulation widely used in clinical practice to treat ischemic stroke (IS). However, there remains a lack of high-quality clinical evidence to support its efficacy in decreasing ischemic stroke recurrence rates and improving clinical outcomes.

Aim Of The Study: To evaluate JPFZF's safety and effectiveness for vascular event recurrence rates and the clinical prognosis of IS.

The Modulation of the Pore Structure in Porous Carbon by Metal Salts and Its Application for Joining Silicon Carbide Ceramics.

In this work, the metal salts were introduced into the resin-solvent gel system to leverage their ortho-substitution effect, thereby accelerating the polymerization-induced phase separation process. Subsequent in-situ carbonization resulted in the preparation of porous carbon materials with three-dimensional interconnected pores. By precisely tuning the parameters of the resin-solvent-metal ion system, control over the pore structure of the porous carbon was achieved, with a porosity range of 16.

Intraspinal ASPSCR1::TFE3 rearranged tumor with nerve differentiation.

The ASPSCR1::TFE3 rearrangement has been described in alveolar soft part sarcoma, MiT family translocation renal cell carcinomas as well as perivascular epithelioid cell tumors (PEComas). However, this rearrangement has not been reported in the primary spinal canal. Here, we report a case of an 18-year-old male who had pain in his left lower limb for 2 months.