Mitochondrial Genome and RNA Editing Tissue Specificity of .

Background: , a medicinally important species that is rich in bioactive compounds, lacks a characterized mitochondrial genome, despite nuclear and chloroplast assemblies. We sequenced and annotated its mitochondrial genome to elucidate its genetic foundations and evolutionary mechanisms.

Methods: Assembly using Illumina short-reads and Nanopore long-reads was used to characterize the mitochondrial genome.

Modified Huangqi Shengmai Yin enhances ruminal microbiome and metabolites activity in dairy cows with subclinical mastitis.

Introduction: Bovine mastitis, especially subclinical mastitis (SCM), with minimal clinical signs, is detrimental due to its resistance to treatment, recurrence, and substantial economic impact on global dairy industry. The modified form of Huangqi Shengmai Yin (HSY), classical traditional herbal medicine renowned for its effects in antimicrobial and circulatory-enhancing and thus beneficial for subclinical mastitis, has been developed for treatment attempt of SCM, yet its therapeutic effect and mechanism remains unclear. This study aims to investigate the therapeutic effects of mHSY on SCM in cows, and elucidate its potential therapeutic mechanism.

Cross-matrix multi-omics profiling identifies host-microbe interactions and diagnostic signatures in bovine subclinical mastitis.

Subclinical mastitis (SCM) is a widespread but frequently undetected condition in dairy cows, leading to reduced milk quality and compromised animal health. This study utilizes an integrated multi-omics strategy encompassing metabolomics and microbiome analyses to investigate the systemic effects of SCM across four biological matrices: blood, milk, feces, and rumen fluid. Our findings reveal significant alterations in hematological and biochemical parameters, with key biomarkers such as digalacturonic acid and N-ε-methyl-L-lysine indicating systemic metabolic and immune dysregulation.

Factors influencing the birth weight of ART-conceived offspring.

Since the birth of the first infant conceived through assisted reproductive technology (ART) in 1978, over 10 million individuals worldwide have been born using these methods. ART has offered hope to many facing infertility. However, concerns remain regarding potential health risks in ART-conceived offspring.

The study on the function of the receptor kinase BnFERONIA of Brassica napus in regulating salt tolerance and ABA tolerance.

CrRLK1L receptor kinases, particularly FERONIA (FER), play critical roles in plant stress adaptation and hormonal signaling, yet their functional characterization in economically vital crops like Brassica napus remains limited. Here, we identified 108 CrRLK1L family members through genome wide screening in B. napus and focused on BnFERONIA, a homolog evolutionarily conserved with FERONIA orthologs in Arabidopsis and related crucifers.

[Association between obesity and the risk of microvascular complications in Yinzhou District, Ningbo adults with type 2 diabetes mellitus].

Objective: To investigate the association between various obesity indices and the risk of developing microvascular complications in adult patients with Type 2 diabetes(T2DM), using cohort data derived from Yinzhou District Health Big Data Platform of China.

Methods: This study included adult patients with type 2 diabetes(T2DM) who were enrolled between January 1, 2008, and December 31, 2013, in Yinzhou District, Ningbo, and did not have any microvascular complications at baseline. Data collection encompassed demographic characteristics, lifestyle behaviors, laboratory test result, and physical examination findings, obtained at both baseline and during follow-up periods through structured epidemiological surveys and clinical assessments.

Associations between meteorological factors and pregnancy complications during different pregnancy trimesters: a multicenter retrospective study in eastern China.

Background: Previous studies have demonstrated the effects of ambient temperature on the risk of pregnancy complications. However, the associations between multiple meteorological factors and pregnancy complications have rarely been studied.

Methods: We carried out a retrospective study on the impacts of meteorological factors on pregnancy complications in different trimesters in Ningbo, China, from 2013-2023.

Host taxonomy and environment shapes insectivore viromes and viral spillover risks in Southwestern China.

Background: Zoonotic viruses originating from small mammals pose significant challenges to public health on a global scale. Insectivores, serving as natural reservoirs for a diverse array of zoonotic viruses, are known to carry a multitude of viral species. However, compared to the extensive research conducted on rodents (Rodentia) and bats (Chiroptera), the role of insectivores in harboring and transmitting unknown pathogens remains underexplored, which may lead to a severe underestimation of their contributions and impact to global public health.

Study on the High-Speed Penetration and Shear Failure Mechanism of Beach Sand.

In this paper, the contact parameters of beach sand are calibrated based on the discrete element method and the optimal design method, and the obtained parameters by calibration are used as input for the angle of repose simulation. The relative error between angle of repose simulation results and experimental results is 3.27%.

Time-divided hypoglycemia management derived by continuous glucose monitoring may reduce microvascular diseases in type 2 diabetes patients.

Purpose: To assess the association between hypoglycemia exposure and the risk of developing microvascular diseases (MVDs) in type 2 diabetes patients via continuous glucose monitoring (CGM), aiming to identify optimal hypoglycemia thresholds for improved glucose management.

Methods: A total of 174 type 2 diabetes patients underwent 2 weeks of CGM. Binary multivariate logistic regression assessed the association between hypoglycemia exposure and MVDs prevalence at thresholds of 3.

A Density Functional Theory Study on the Effects of Silver Doping on the Properties and Flotation Behavior of Jamesonite.

Silver (Ag) is a precious and valuable metal, and it has many carrier minerals. Through LA-ICP-MS analysis, it was found that jamesonite not only contains lead (Pb) and antimony (Sb) as precious metals but also trace amounts of Ag. In practice, the flotation method is generally used to recover these metals.

The investigation of molecular epidemiological characteristics and resistance mechanism of tigecycline resistant from a large teaching hospital in southwest China, Chongqing.

Background: is one of the main pathogens of nosocomial infection, among which carbapenems can be used for multidrug-resistant . However, in the past decade, the resistance rate of carbapenem-resistant has increased yearly. Tigecycline has good antibacterial activity in treating severe bacterial infections, but the reports of tigecycline resistance are increasing.

An Experimental and Quantum Chemical Calculation Study on the Performance of Different Types of Ester Collectors.

Ester collectors have rapidly developed into the main flotation collectors for copper sulfide minerals since they were developed. In this study, the collecting performance of four collectors, O-isopropyl-N-ethyl thionocarbamate ester (IPETC), 3-pentyl xanthate acrylate ester (PXA), O-isobutyl-N-allyl-thionocarbamate (IBALTC), and O-isobutyl-N-isobutoxycarbonyl-thionocarbamate (IBIBCTC), was investigated through microflotation tests, microcalorimetric measurements, and quantum chemical calculations. The results of the microflotation tests show that IBALTC and IPETC have stronger collecting abilities than IBIBCTC and PXA; the order of collecting ability is IBALTC > IPETC > IBIBCTC > PXA.

Sleep features and the risk of type 2 diabetes mellitus: a systematic review and meta-analysis.

Objective: This study aimed to assess the associations between multidimensional sleep features and type 2 diabetes mellitus (T2DM).

Methods: We conducted a systematic search across the PubMed, Embase, Web of Science, and Scopus databases for observational studies examining the association between nighttime sleep duration, nighttime sleep quality, sleep chronotype, and daytime napping with type 2 diabetes mellitus (T2DM), up to October 1, 2024. If < 50%, a combined analysis was performed based on a fixed-effects model, and vice versa, using a random-effects model.

The factors that influence the diagnostic accuracy and sample adequacy of EUS-guided tissue acquisition for the diagnosis of solid pancreatic lesions.

Background And Objectives: EUS-guided tissue acquisition (EUS-TA) is the preferred method to acquire pancreatic cancer (PC) tissues. The factors associated with false-negative outcomes and inadequate samples should be explored to gain an understanding of EUS-TA.

Methods: The patients who underwent EUS-TA for suspected solid PC but whose results were false-negative were analyzed.

Antimicrobial susceptibilities, resistance mechanisms and molecular characteristics of toxigenic Clostridioides difficile isolates in a large teaching hospital in Chongqing, China.

Objectives: Clostridioides difficile ranks among the primary sources of healthcare-related infections and diarrhoea in numerous nations. We evaluated the drug susceptibility and resistance mechanisms of C. difficile isolates from a hospital in Chongqing, China, and identified resistance rates and resistance mechanisms that differed from previous findings.

Tribological behavior of graphene/h-BN vdW heterostructures: the role of defects at the BN layer.

Molecular dynamics simulations and first principles calculations were performed to study the tribological behavior of graphene/h-BN (G/h-BN) heterostructures with vacancy and Stone-Wales (SW) defect under uniform normal load, revealing the mechanism of the effect of defect types on friction, and discussing the coupling effect of temperature and interfacial defects on the tribological behavior of G/h-BN heterostructures. Under the normal force of 0.2 nN/atom, the friction force of the four systems is 0.

Efficacy of an herbal formula Guixiong Yimu San in preventing retained placenta and improving reproductive performance in cows.

Retained placenta is a common health issue, and appropriate prevention strategies are effective in postpartum health management. This study aimed to evaluate whether early intervention using GYS can prevent retained placenta and puerperal metritis, as well as enhance reproductive outcomes in cows. Each bovine in the GYS group (n = 591) received a single prophylactic dose of GYS (0.

Global profiling of protein S-palmitoylation in the second-generation merozoites of Eimeria tenella.

The intracellular protozoan Eimeria tenella is responsible for avian coccidiosis which is characterized by host intestinal damage. During developmental cycle, E. tenella undergoes versatile transitional stages such as oocyst, sporozoites, merozoites, and gametocytes.

Molecular epidemiology and resistance mechanisms of tigecycline-non-susceptible Acinetobacter baumannii isolated from a tertiary care hospital in Chongqing, China.

We studied 34 isolates of Tigecycline-Non-Susceptible A. baumannii (TNAB) obtained from clinical specimens at a large tertiary care hospital in Chongqing, China. These 34 strains belonged to 8 different clones including ST195 (35.

Multi-group symbiotic evolutionary mechanisms of a digital innovation ecosystem: Numerical simulation and case study.

In the digital innovation ecosystem, the symbiosis mode formed between ecosystem members not only relates to their survival and development but also affects the ecosystem's symbiosis evolution mechanism. Based on symbiosis theory, this study first explores the evolutionary equilibrium strategy and its stability for three types of populations-core enterprises, digital platforms, and university research institutes-and then uses numerical simulation and a case study to explore the symbiotic evolution mechanism of the digital innovation ecosystem. The results show that: First, the digital innovation ecosystem is a complex adaptive system in which the three types of populations form different symbiotic relationships under different symbiotic modes and conduct symbiotic activities, such as value co-creation, to characterize the unique symbiotic evolutionary structure.

Cecropin A Alleviates LPS-Induced Oxidative Stress and Apoptosis of Bovine Endometrial Epithelial Cells.

Dairy cows receiving a prolonged high-concentrate diet express an elevated concentration of lipopolysaccharides (LPSs) in the peripheral blood circulation, accompanied by a series of systemic inflammatory responses; however, the specific impacts of inflammation are yet to be determined. Cecropin-like antimicrobial peptides have become a research hotspot regarding antimicrobial peptides because of their excellent anti-inflammatory activities, and cecropin A is a major member of the cecropin family. To elucidate the mechanism of cecropin A as anti-inflammatory under the condition of sub-acute ruminal acidosis (SARA) in dairy cows, we induced inflammation in bEECs with LPS (10 µg/mL) and then added cecropin A (25 µM).

Review of Theoretical and Computational Studies of Bulk and Single Atom Catalysts for H S Catalytic Conversion.

Catalytic conversion of hydrogen sulfide (H S) plays a vital role in environmental protection and safety production. In this review, recent theoretical advances for catalytic conversion of H S are systemically summarized. Firstly, different mechanisms of catalytic conversion of H S are elucidated.