Mapping evolutionary paradigm of bovine viral diarrhea virus associated with different organizations of nucleotide.

The non-structural protein (Npro) of bovine viral diarrhea virus (BVDV) is a crucial virulence factor that impairs the host's antiviral immune response and facilitates virus production. This study establishes a foundation for understanding how different selective pressures influence the formation of nucleotide pairs, synonymous codon, and context-dependent codon bias (CDCB) in BVDV . BVDV genotype 1 exhibits a greater number of subgenotypes compared to other genotypes, yet its overall nucleotide usage bias in is stronger.

Establishment of a TaqMan qPCR method with MGB probe for the specific detection of BVDV field strains circulating in China.

Bovine viral diarrhea virus (BVDV), a highly mutable pathogen, poses a significant threat to the cattle industry in China. Therefore, the development of a rapid, sensitive, and specific diagnostic assay is essential for effective surveillance and control. In this study, a TaqMan real-time quantitative PCR (qPCR) assay utilizing a minor groove binder (MGB) probe was developed for the detection of BVDV, with a focus on strains currently circulating in China.

Tetraamino-driven hydrogen-bonded networks: selective self-assembly of energetic materials.

Hydrogen bonding is crucial in the self-assembly of energetic materials. However, such dynamic networks suffer from selectivity difficulties and are susceptible to interference from the multiple assembly components. In this work, we proposed a polyamino energetic framework 2,5,6,9-tetraamino-pyrazino[2,3-]pyridazine ().

Effects of synonymous codons with optimization / deoptimization in nucleoprotein (NP) gene of influenza A virus on interaction between NP and tripartite motif protein 25 (TRIM25).

Nucleoprotein (NP), which performs multiple functions, participates in the life cycle of the influenza A virus (IAV). Although the synonymous codon usage of IAV NP exhibits a relatively variable pattern, it demonstrates a significant bias. Here, we first validated the directional interaction between IAV NP and tripartite motif protein 25 (TRIM25).

Multi-omics characterization of prognostic signature and novel molecular subtypes associated with anoikis and in vitro validation of CDKN2A in thyroid carcinoma.

Background: Anoikis plays a crucial role in thyroid carcinoma (THCA). Recent evidence has suggested CDKN2A as a key anoikis-related gene (ARG) associated with THCA. However, the multi-omics regulatory landscape of ARGs and their potential to define molecular subtypes with distinct therapeutic vulnerabilities remain unexplored.

Introducing Azide into Fully Nitrated Systems: Charge Mutations toward Advanced Initiators.

Over the past century, the continuous search for advanced initiators has been a major challenge in the field of high-energy-density materials. In this study, we revealed a new strategy for constructing initiators by inducing intramolecular charge mutations, achieved by introducing a strongly charge-positive azide group into highly charge-negative fully nitrated systems. Two azide functionalized fully nitrated compounds3-nitro-5-azide-1-trinitromethyl-1H-1,2,4-triazole () and 3-nitro-6-azide-1-trinitromethyl-1H-[1,2,4]-triazolo-[3,2-]-[1,2,4]-triazole ( with α and β polymorphs)were designed and synthesized.

PLZF-expressing CD4 T cells promote tissue-resident memory T cells in breaking immune tolerance in allergic asthma via IL-15/IL-15Rα signaling.

Background: Allergic asthma is a chronic airway disease characterized by an allergic response and altered immune tolerance. CD4 tissue-resident memory T (TRM) cells are crucial in the chronic and relapsing pathogenesis of asthma. Furthermore, promyelocytic leukemia zinc finger (PLZF) is an essential transcription factor involved in asthmatic tolerance and has been implicated in the regulation of CD4CD44 memory T cells.

Screening of cytokines-cytokine receptor-associated genes in childhood asthma based on bioinformatics.

Purpose: To develop efficient diagnostic and treatment approaches, gaining an in-depth knowledge of the molecular mechanisms and potential targets causing childhood asthma is of utmost significance.

Methods: Childhood asthma datasets were obtained from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) between asthmatic child and healthy people were screened by the Limma package.

Early RSV infection aggravates asthma-related Th2 responses by increasing the number of CD4 TRM cells through upregulation of PLZF.

Respiratory syncytial virus (RSV) infection is correlated with the chronic pathogenesis and exacerbation of asthma. However, the mechanism remains unclear. In this study, acute and memory (Mem) asthma models with early RSV infection are established to explore the persistence of the effects of RSV infection on asthma.

Construction and Preparation of the Novel ADN/CL-20 Cocrystal via Directional Hydrogen Bonding Design for Turning Hygroscopicity.

Ammonium dinitramide (ADN), as a novel and environmentally friendly oxidizer, has strong hygroscopicity when exposed to high-humidity air, which seriously hinders its application in solid propellants. Modification of oxidizers by cocrystallization is an effective strategy to improve the hygroscopicity of energetic components. In this paper, the theoretical simulation of ADN/CL-20 cocrystals was developed via a directional hydrogen bonding design to establish a cocrystal with improved hygroscopicity.

YiQi GuBen capsule alleviates OVA-induced asthma through improving mitochondrial dysfunction.

This study aims to explore the effect of YiQi GuBen capsule on improving mitochondrial dysfunction in an animal model of asthma. The mice ( = 8) were divided into four groups including control (NC), ovalbumin (OVA), dexamethasone (OVA + DEX), and YiQi GuBen (OVA + YQGB) groups. Firstly, we established an OVA-induced mouse asthma model except for the NC group, which then were treated with dexamethasone and YiQi GuBen capsule.

Association between parental occupational exposure and the risk of asthma in offspring: A meta-analysis and systematic review.

Background: Previous epidemiological studies have shown inconsistent results regarding the relation between the risk of asthma in offspring and parental occupational exposure. Therefore, we conducted a comprehensive and systematic collection of currently available epidemiological data to quantify the correlation between the 2.

Methods: Related studies published before March 2023 were identified through searches of the Cochrane Library, Embase, PubMed, and Web of Science databases.

Enhanced Thermal Decomposition and Safety of Spherical CL-20@MOF-199 Composites via Micro-Nanostructured Self-Assembly Regulation.

The characteristics of high burning rate, high energy output, and low pressure exponent have always been the focus of development in the field of composite solid rocket propellants. In this paper, a metal-organic framework (MOF-199) compound is introduced to prepare micro-nanospherical CL-20@MOF-199 composites via the spray-drying self-assembly technique to reach the above goals. MOF-199, which acts as an attractive combustion catalyst and a safety regulator, is uniformly coated on the surface of CL-20 with close interface contact between particles, effectively accelerating the thermal decomposition of CL-20 and ensuring safety performance.

A new strategy to prepare high-performance copper azide film for micro-initiator.

Copper azide (CA) has gradually become the chosen priming agent for microexplosive devices as a lead-free green priming agent. However, charge loading is challenging due to its high electrostatic sensitivity, severely limiting its practical application. In this study, copper hydroxide particles were evenly coated on the surface of carbon fiber using electrospinning and quick hot-pressing, and CA-based composites with uniform load were created using theazide technique while keeping good film characteristics.

Effect of hydrogen/oxygen therapy for ordinary COVID-19 patients: a propensity-score matched case-control study.

Background: Hydrogen/oxygen therapy contribute to ameliorate dyspnea and disease progression in patients with respiratory diseases. Therefore, we hypothesized that hydrogen/oxygen therapy for ordinary coronavirus disease 2019 (COVID-19) patients might reduce the length of hospitalization and increase hospital discharge rates.

Methods: This retrospective, propensity-score matched (PSM) case-control study included 180 patients hospitalized with COVID-19 from 3 centers.

Single-cell transcriptomes reveal heterogeneity of chlorine-induced mice acute lung injury and the inhibitory effect of pentoxifylline on ferroptosis.

To investigate the effect of pentoxifylline (PTX) on Chlorine (Cl)-induced acute lung injury (ALI) by single-cell RNA sequencing (scRNA-seq). Female BALB/c mice were exposed to Cl at 400 ppm for 15 min. H&E staining was used to observe the degree of lung injury.

Diffusion simulation and risk assessment model establishment of chlorine gas leakage based on terrain conditions.

This study researches the impact of terrain factors on chlorine gas diffusion processes based on SLAB model. Simulating the law of wind speed changing with altitude by calculating the real-time speed with vertical height combing actual terrain data, and integrating the influence of terrain on wind speed by using Reynolds Average Navier-Stokes (RANS) algorithm, K-turbulence model, and standard wall functions, then plotting the gas diffusion range in the map with terrain data according to the Gaussian-Cruger projection algorithm and dividing the hazardous areas according to the public exposure guidelines (PEG). The accidental chlorine gas releases near Lishan Mountain, Xi'an City, were simulated by the improved SLAB model.

Platinum-based adjuvant therapy was efficient for triple-negative breast cancer: a meta-analysis from randomized controlled trials.

Triple-negative breast cancer (TNBC) is the most aggressive breast cancer. Neoadjuvant chemotherapy was widely accepted for treating TNBC. This systematic review and meta-analysis aimed to evaluate the efficacy, safety, and survival benefit of platinum-based adjuvant therapy (PBAT) in treating TNBC.

Heterogeneity of T cells and macrophages in chlorine-induced acute lung injury in mice using single-cell RNA sequencing.

Objective: Chlorine (Cl), as an asphyxiant toxicant, induced poisoning incidents and acute lung injury (ALI) occur frequently. The specific pathogenesis of Cl-induced ALI remains unclear. Immune cells play an important role in the process of lung damage.

Evaluating the ability of the NLHA2 and artificial neural network models to predict COVID-19 severity, and comparing them with the four existing scoring systems.

To improve the identification and subsequent intervention of COVID-19 patients at risk for ICU admission, we constructed COVID-19 severity prediction models using logistic regression and artificial neural network (ANN) analysis and compared them with the four existing scoring systems (PSI, CURB-65, SMARTCOP, and MuLBSTA). In this prospective multi-center study, 296 patients with COVID-19 pneumonia were enrolled and split into the General-Ward-Care group (N = 238) and the ICU-Admission group (N = 58). The PSI model (AUC = 0.

Extracellular Vesicle-Encapsulated MicroRNA-375 from Bone Marrow-Derived Mesenchymal Stem Cells Inhibits Hepatocellular Carcinoma Progression through Regulating HOXB3-Mediated Wnt/-Catenin Pathway.

Nowadays, microRNA-375 (miR-375) has been implicated in many types of cancers, including hepatocellular carcinoma (HCC), and the functions of miRNAs encapsulated by extracellular vesicles (EV) in HCC progression have also been extensively investigated. In this research, we aimed to probe into the mechanism of EV-encapsulated miR-375 from bone marrow-derived mesenchymal stem cells (BM-MSCs) in HCC progression. At first, miR-375 expression in HCC tissues and cells was detected using RT-qPCR, and miR-375 was overexpressed to specify the effects of miR-375 on the malignant phenotype of HCC cells.

Improving diacetyl production in Corynebacterium glutamicum via modifying respiratory chain.

To explore the suitability of Corynebacterium glutamicum as a chassis for diacetyl production from glucose, diacetyl metabolic pathway and the respiratory chain were linked to achieve redox balance. The carbon flux was redirected from pyruvate to diacetyl by overexpressing the α-acetolactate synthase, in combination with disruption the biosynthetic pathways of lactate, acetoin, 2,3-butanediol and acetate in C. glutamicum ATCC 13032.

A randomized, double-blind, placebo-controlled, multicenter clinical trial for efficacy and safety of traditional Chinese medicine combined with antibiotics in the treatment of bacterial pneumonia in children.

Background: Pneumonia is the second leading cause of death in children worldwide after preterm birth and certification. Bacteria, viruses, mycoplasma, and other microorganisms are known to be the main causes of pneumonia, of which bacterial pathogenic factors account for 12.5% of cases.