Comparison of short- and long-term outcomes among total laparoscopic, laparoscopic-assisted, and open total gastrectomies for advanced gastric cancer patients after neoadjuvant chemotherapy: a multicenter retrospective cohort study.

Objective: To evaluate the short- and long-term outcomes of totally laparoscopic, laparoscopic-assisted, and open total gastrectomy in gastric cancer patients after neoadjuvant therapy.

Methods: This multicenter retrospective cohort study was conducted to collect clinical data from 289 patients who underwent total radical gastrectomy after neoadjuvant therapy at five centers. The patients were divided into three groups according to the surgical method they received: totally laparoscopic, laparoscopic-assisted, and open groups.

Isolation and pathogenicity of the novel porcine reproductive and respiratory syndrome virus 1 strain ZJ01 in South China.

Introduction: The emergence and widespread dissemination of novel Porcine Reproductive and Respiratory Syndrome Virus type 1 (PRRSV-1) strains in China pose significant challenges, leading to substantial clinical infections within swine populations.

Methods: In this study, we isolated a novel PRRSV-1 strain, designated ZJ01.

Results: Whole-genome sequencing revealed a genome length of 15,125 bp.

Isolation and Pathogenicity of an Emerging Highly Virulent CSFV 2.1c Strain in South China.

Classical swine fever (CSF) is an infectious disease caused by classical swine fever virus (CSFV), which is endemic in many areas of China, causing serious economic losses to pig farms. Currently, 2.1 subgenotype strains are predominantly prevalent in China.

The Application of Artificial Intelligence-Based Bone Marrow Cell Analysis System in Pediatric Hematological Diseases.

Introduction: The clinical diagnosis of hematological diseases depends on the differential count of nucleated cells on the bone marrow (BM) smears, and an artificial intelligence (AI)-based system was applied to automatically classify BM nucleated cells in pediatric hematological disease samples in this study.

Methods: The BM aspirate smears were collected from 213 pediatric patients (under 18 years old) at Harbin Medical University Affiliated Sixth Hospital from October 2023 to June 2024. The entire smear of BM was scanned by a ×40 objective lens to obtain complete digital images using an automated analysis method named Morphogo.

Clinical outcomes of restricted kinematic alignment versus mechanical alignment in total knee arthroplasty: a meta-analysis of randomized controlled trials.

Background: Restricted Kinematic Alignment (rKA) is a well-established technique for total knee arthroplasty (TKA) that aims to approximate natural knee motion within defined safety boundaries. This meta-analysis aims to evaluate the clinical efficacy of rKA compared to mechanical alignment (MA) in TKA.

Method: A comprehensive search was conducted in the PubMed, Embase, Web of Science, and Cochrane Library databases for randomized controlled trials (RCTs) published from the inception of these databases until December 6, 2024.

Self-Assembled Monolayers for Perovskite Solar Cells: Molecular Design and Chemical Synthesis.

Self-assembled monolayers (SAMs) have been a game-changer for perovskite solar cells (PSCs), significantly boosting their power conversion efficiency (PCE) to 27% in recent years. This breakthrough has garnered unprecedented attention, leading to significant advancements in the design and synthesis of SAM molecules (SAMols). Additionally, SAMs hold significant promise in addressing the lifetime of PSCs and facilitating their commercialization in terms of diversity, flexibility, and multifunctionality.

Characterization and Pathogenicity of the Novel Porcine Reproductive and Respiratory Syndrome Virus 1 Strain SL-01 in China.

Currently, PRRSV-1 causes a large number of clinical infections in Chinese swine herds, and the prevalence of new strains has presented great challenges. In this study, the novel PRRSV-1 strain SL-01 was isolated, with a genome length of 14,978 bp, and genetic evolution analysis revealed that it belonged to a new subtype branch. Sequence homology analysis showed that the strain was only 82.

Global, regional, and national epidemiology of rheumatoid arthritis among people aged 20-54 years from 1990 to 2021.

Rheumatoid arthritis (RA) is a global epidemic. We conducted a cross-sectional study using the Global Burden of Disease (GBD) 2021 dataset to examine RA trends in patients aged 20-54 years worldwide. Key outcomes included incidence, mortality, and disability-adjusted life years (DALYs), as well as trends over time, stratifying by region, country, age, sex, and Sociodemographic Index (SDI).

Enhanced efficiency nitrogen fertilizers suppress soil methane uptake from a mulch drip-irrigation system in arid region.

Limited information is available on the impact of Enhanced Efficiency Nitrogen Fertilizers (EENFs) on soil methane (CH) uptake and its relationship with diffusion in dryland farming. To address this, we conducted a two-year cotton (Gossypium hirsutum L.) field experiment under drip irrigation in arid northwestern China.

Mitigating life-cycle multiple environmental burdens while increasing ecosystem economic benefit and crop productivity with regional universal nitrogen strategy.

Introduction: Nitrogen fertiliser is critical for increasing crop yields worldwide, but excessive use causes significant N losses in various forms and subsequent environmental issues, such as greenhouse gas (GHG) emissions. Establishing regional universal nitrogen strategy (RUNs) is indispensable for technology adoption, resource conservation, and pollution mitigation in crop production.

Objectives: This study aims to develop a regional universal nitrogen fertilizer strategy to address variations in N application effectiveness, balancing agricultural productivity with environmental and eco-economic benefits.

Metabolic Engineering of for Producing Different Types of Triterpenoids.

Triterpenoids widely exist in nature with diverse structures and possess various functional properties and biological effects. However, research on triterpenoids biosynthesis in is still limited to squalene, which restricts the development of to produce high-value triterpenoids. In this study, was developed as an efficient and flexible platform for the biosynthesis of different types of triterpenoids.

African swine fever virus enhances viral replication by increasing intracellular reduced glutathione levels, which suppresses stress granule formation.

African swine fever virus (ASFV) is a DNA virus that has significantly impacted the global swine industry. Currently, there are no effective therapies or vaccines against ASFV. Stress granules (SGs), known for their antiviral properties, are not induced during ASFV infection, even though reactive oxygen species (ROS) are generated.

Evaluation of the cross-protective effect of VR2332 modified live virus vaccine against a recombinant NADC34-like porcine reproductive and respiratory syndrome virus.

In recent years, NADC34-like strains of porcine reproductive and respiratory syndrome virus have gradually emerged as mainstream strains on Chinese pig farms. These strains have high mutation rates and can recombine with local strains, representing great challenges to prevention and control efforts. Previously, a new recombinant NADC34-like subtype strain was isolated in our laboratory.

Release of phosphorus and metal(loid)s from manured soils to floodwater during a laboratory simulation of snowmelt flooding.

Phosphorus (P) and metal accumulation in manured agricultural soils and subsequent losses to waterways have been extensively studied; however, the magnitudes and the factors governing their losses during spring snowmelt flooding are less known. We examined the P and metal release from long-term manured soil to floodwater under simulated snowmelt flooding with recent manure additions. Intact soil columns collected from field plots located in Randolph, Southern Manitoba, 2 weeks after liquid swine manure treatments (surface-applied, injected, or control with no recent manure addition) were flooded and incubated for 8 weeks at 4 ± 1°C to simulate snowmelt conditions.

Co-benefits for net carbon emissions and rice yields through improved management of organic nitrogen and water.

Returning organic nutrient sources (for example, straw and manure) to rice fields is inevitable for coupling crop-livestock production. However, an accurate estimate of net carbon (C) emissions and strategies to mitigate the abundant methane (CH) emission from rice fields supplied with organic sources remain unclear. Here, using machine learning and a global dataset, we scaled the field findings up to worldwide rice fields to reconcile rice yields and net C emissions.

African swine fever virus maintains de novo global cellular protein synthesis and inhibits stress granules formation via dephosphorylating eIF2α.

African swine fever virus (ASFV) has caused enormous economic losses since its first reported detection, and there is still no effective vaccines or drug treatment. During infection, viruses may employ various strategies, such as regulating the host endoplasmic reticulum stress/unfolded protein response or the formation of stress granules (SGs), to form an optimal environment for virus replication. However, how ASFV infection regulates host endoplasmic reticulum stress, eIF2α-regulated protein synthesis, and the formation of SGs remains unclear.

A Longitudinal Study of NADC34-Like Strains in an Intensive Farm Unravels Divergent Evolution.

NADC34-like porcine reproductive and respiratory virus (PRRSV) has had a significant impact on the pig industry, particularly in China. However, the evolutionary characteristics and pathogenicity of NADC34-like PRRSV strains within intensive farming systems are not well understood, particularly regarding the biological characteristic variation of successive outbreaks on a farm. In this study, we conducted continuous surveillance in an intensive farm that experienced a PRRSV outbreak.