[Rapid screening of 111 pesticides and veterinary drugs in livestock and poultry meat by ultra performance liquid chromatography-quadrupole-time of flight mass spectrometry].

The consumption of agricultural products has increased in recent years owing to abundant production and improved living standards. Veterinary drugs are highly commercialized and widely used in animal husbandry to ensure animal health and production performance. Moreover， pesticides can become enriched during animal breeding， resulting in animal-derived food pollution through foraging， drinking， and environmental disinfection that can potentially damage human health.

Multiomic identification of senescent stem cell populations critical for osteoarthritis progression and therapy in subchondral bones.

Osteoarthritis (OA) is a challenging degenerative joint disease with limited treatment options. Subchondral bone plays a critical role in maintaining joint homeostasis and influencing OA progression. Here, we investigated the role of senescence in mesenchyme-derived stem/progenitor cells (MDSPCs) during OA progression, aiming to identify potential therapeutic targets.

Clinical outcomes of restricted kinematic alignment versus mechanical alignment in total knee arthroplasty: a meta-analysis of randomized controlled trials.

Background: Restricted Kinematic Alignment (rKA) is a well-established technique for total knee arthroplasty (TKA) that aims to approximate natural knee motion within defined safety boundaries. This meta-analysis aims to evaluate the clinical efficacy of rKA compared to mechanical alignment (MA) in TKA.

Method: A comprehensive search was conducted in the PubMed, Embase, Web of Science, and Cochrane Library databases for randomized controlled trials (RCTs) published from the inception of these databases until December 6, 2024.

[Preparation and Evaluation of Clinical-Grade Human Umbilical Cord-Derived Mesenchymal Stem Cells with High Expression of Hematopoietic Supporting Factors].

Objective: To prepare clinical-grade human umbilical cord-derived mesenchymal stem cells (hUC-MSC) with high expression of hematopoietic supporting factors and evaluate their stem cell characteristics.

Methods: Fetal umbilical cord tissues were collected from healthy postpartum women during full-term cesarean section. Wharton's jelly was mechanically separated and hUC-MSCs were obtained by explant culture method and enzyme digestion method in an animal serum-free culture system with addition of human platelet lysate.

Modulating oral microbiota ameliorates hypobaric hypoxia-induced anxiety- and depression-like behaviors in mice.

Background: Hypobaric hypoxia exposure (HHE) often causes neuropsychiatric disorders. Due to its complex mechanism, efficient strategies for alleviating HHE-induced anxiety- and depression-like behaviors remain limited.

Aim: To characterize alterations in the oral and gut microbiota following HHE and to explore a potential microbiota-based intervention to mitigate associated psychiatric symptoms.

Extensive Dynamic Functional Network Connectivity Alterations in Diabetic Retinopathy Among Patients with Type 2 Diabetes.

Background: Diabetic retinopathy (DR) is a prevalent microvascular complication of diabetes. Prior neuroimaging research has indicated that patients with DR exhibit diverse levels of disrupted brain function alongside a variety of ocular symptoms. Nevertheless, past investigations have predominantly focused on static brain activity changes, leaving uncertainties regarding the modifications in dynamic large-scale brain networks among DR patients.

Modulation of senescent Lepr skeletal stem cells via suppression of leptin-induced STAT3‒FGF7 axis activation alleviates abnormal subchondral bone remodeling and osteoarthritis progression.

Background: Recent studies have suggested that targeting senescent cells in joint tissues may alleviate osteoarthritis (OA) progression. However, this strategy encounters significant challenges, partially due to the high degree of cellular heterogeneity in osteoarthritic tissues. Moreover, little information is available on the role of skeletal stem cell (SSC) senescence, as compared to differentiated cells, in OA progression.

Analysis of diroximel fumarate data for patients with relapsing forms of multiple sclerosis using related adverse events from the FDA adverse reporting system.

Background: Diroximel fumarate (DRF) is an oral fumarate used to treat relapsing forms of multiple sclerosis (RMS). This study comprehensively analyzed the adverse events (AEs) associated with DRF for treating RMS based on data from the FDA Adverse Event Reporting System (FAERS) database.

Research Design And Methods: This study collected data on AEs associated with DRF treatment of RMS from the FAERS database between 2019 and 2024.

[Establishment and Application of an in Vitro Cellular Model of Adipogenic Differentiation of Bone Marrow Mesenchymal Stem Cells with Serum Injury in aGVHD Mouse].

Objective: To establish an cell model simulating acute graft-versus-host disease (aGVHD) bone marrow microenvironment injury with the advantage of mouse serum of aGVHD model and explore the effect of serum of aGVHD mouse on the adipogenic differentiation ability of mesenchymal stem cells (MSCs).

Methods: The 6-8-week-old C57BL/6N female mice and BALB/c female mice were used as the donor and recipient mice of the aGVHD model, respectively. Bone marrow transplantation (BMT) mouse model (=20) was established by being injected with bone marrow cells (1×10 per mouse) from donor mice within 4-6 hours after receiving a lethal dose (8.

[The Enhancing Effects and Underlying Mechanism of Ionizing Radiation on Adipogenic Differentiation of Mesenchymal Stem Cells via Regulating Oxidative Stress Pathway].

Objective: To investigate the effects and underlying mechanism of ionizing radiation on the adipogenic of mesenchymal stem cells (MSCs).

Methods: Mouse MSCs were cultured in vitro and treated with 2 Gy and 6 Gy radiation with Co, and the radiation dose rate was 0.98 Gy/min.

Disrupted topology of the functional white matter connectome in thyroid-associated ophthalmopathy.

Background: This study aims to investigate the changes in the topological organization of WM functional connectivity in individuals with TAO, providing a novel and insightful perspective on the functional disruptions that characterize this condition.

Methods: This study utilized resting-state functional Magnetic Resonance Imaging (rs-fMRI) to capture blood-oxygen-level-dependent (BOLD) signals and T1-weighted images from patients with TAO and healthy control subjects. Group-level masks for white matter were created to extract WM-related BOLD signals, facilitating the construction of a functional white matter network.

White Matter Function and Network Abnormalities in Patients with Diabetic Retinopathy.

Background: This study aims to explore changes in white matter function and network connectivity in individuals with DR.

Methods: This study included 46 patients with DR and 43 age- and gender-matched healthy control (HC) participants were enrolled in the study. The aim was to investigate inter-group differences in white matter (WM) function and to analyze changes in the WM network among DR patients.

A nomogram prediction of gingival recession in mandibular incisors of orthodontic-orthognathic treated skeletal class III malocclusion with or without PAOO: A retrospective cohort study.

Background: To assess the alterations in gingival thickness and the occurrence gingival recession subsequent to orthodontic-orthognathic treatment of mandibular incisors in skeletal Class III and identify risk factors associated with gingival recession.

Methods: In this retrospective cohort study, we enrolled 33 patients exhibiting skeletal Class III malocclusion, totaling 131 mandibular incisors, who were undergoing orthodontic- orthognathic treatment that did not involve extraction of mandibular teeth. The subjects were categorized into surgery group (S; n = 17; ANB = -5.

Topological Organization of the Brain Network in Patients with Primary Angle-closure Glaucoma Through Graph Theory Analysis.

Primary angle-closure glaucoma (PACG) is a sight-threatening eye condition that leads to irreversible blindness. While past neuroimaging research has identified abnormal brain function in PACG patients, the relationship between PACG and alterations in brain functional networks has yet to be explored. This study seeks to examine the influence of PACG on brain networks, aiming to advance knowledge of its neurobiological processes for better diagnostic and therapeutic approaches utilizing graph theory analysis.

Microgel-based carriers enhance skeletal stem cell reprogramming towards immunomodulatory phenotype in osteoarthritic therapy.

Skeletal stem cells (SSC) have gained attentions as candidates for the treatment of osteoarthritis due to their osteochondrogenic capacity. However, the immunomodulatory properties of SSC, especially under delivery operations, have been largely ignored. In the study, we found that Pdpn and Grem1 SSC subpopulations owned immunoregulatory potential, and the single-cell RNA sequencing (scRNA-seq) data suggested that the mechanical activation of microgel carriers on SSC induced the generation of PdpnGrem1Ptgs2 SSC subpopulation, which was potent at suppressing macrophage inflammation.

TNFAIP3 Derived from Skeletal Stem Cells Alleviated Rat Osteoarthritis by Inhibiting the Necroptosis of Subchondral Osteoblasts.

Recent investigations have shown that the necroptosis of tissue cells in joints is important in the development of osteoarthritis (OA). This study aimed to investigate the potential effects of exogenous skeletal stem cells (SSCs) on the necroptosis of subchondral osteoblasts in OA. Human SSCs and subchondral osteoblasts isolated from human tibia plateaus were used for Western blotting, real-time PCR, RNA sequencing, gene editing, and necroptosis detection assays.

[The Advance of the HSCT Evaluation at Single-Cell Scale --Review].

Hematopoietic stem cell transplantation (HSCT) is one of the effective options for the treatment of irradiation-induced injury on hematopoiesis, malignant hematological diseases, and numerous benign severe hematopathy. However, the cellular composition of the graft for HSCT, as well as the significant events of transplanted HSCs in receipients including HSC homing, engraftment, differentiation, remains to be further elucidated. In recent years, with advances in single-cell techniques, the hematopoiesis has been decoding at single cell scale.