ZAG promotes exercise performance during endurance exercise by lipid utilization in skeletal muscle.

Endurance exercise significantly enhances energy expenditure with lipids serving as a crucial energy source for skeletal muscle during exercise. The adipocytokine Zinc-α2-glycoprotein (ZAG) in endurance exercise remains largely uncertain. This study utilized ZAG knockout and overexpression mice to investigate ZAG's role in regulating lipid metabolism in skeletal muscle during endurance exercise.

UV Mutagenesis Enhances DHA Biosynthesis in Schizochytrium sp. via Metabolic Reprogramming.

Schizochytrium sp., a marine alga prized for docosahexaenoic acid (DHA), was subjected to UV mutagenesis to boost industrial yields. The stable mutant UV1-3 achieved 5.

Tumor-derived PRMT1 suppresses macrophage antitumor activity by inhibiting cGAS/STING signaling in gastric cancer cells.

Gastric cancer (GC) is a common and aggressive malignancy worldwide. Increasing evidence has shown that epigenetic changes are closely related to the development of cancer and tumor-associated macrophages. Here, we report that PRMT1 is a key immunosuppressive factor in GC.

Enhanced performance of algal-bacterial granular sludge in treating wastewater under PFOA stress: Synergistic effects and mechanisms.

The ubiquitous distribution of perfluorooctanoic acid (PFOA) in wastewater poses significant challenges for wastewater treatment systems, yet its impact on granular sludge systems remains poorly understood. This study investigated the performance of aerobic granular sludge (AGS) and algal-bacterial granular sludge (ABGS) systems in terms of stability, nutrient and PFOA removal, enzyme activity, and microbial communities under PFOA stress. Both systems demonstrated tolerance and adsorptive removal of PFOA with enhanced total nitrogen (TN) and slightly decreased total phosphorus (TP) removals.

Effects of supplementary and restricted feeding on meat flavor, lipid metabolism and muscle protein metabolism of Hu sheep.

This study investigated dietary regimens on Hu sheep meat quality. Compared to control, supplementary feeding (SF) increased body weight (63.83 %), eye muscle area (89.

Strategy development for enhancing Trichoderma longibrachiatum biocontrol efficacy based on integrated functional omics.

Biocontrol using beneficial microbes like Trichoderma is an eco-friendly strategy for plant disease management but is limited by poor soil colonization and low secondary metabolite production. This study isolated Trichoderma longibrachiatum Tr-8, which shows strong cellulose degradation and resource competition. Integrated omics revealed Tr-8's preference for cellulose-based carbon, promoting polyketosynthase gene expression.

Oligomeric hyaluronic acid-modified liposomes effectively improved skin permeability and anti-ageing activity of ellagic acid.

To overcome natural skin barrier, deliver ellagic acid (EA) to the dermis, and promote its anti-ageing efficacy, oligomeric hyaluronic acid (HA) modified EA-loaded liposomes (EA-HA-L) were constructed via self-synthesized different molecular weights of HA linked cholesterol (HA-Chol), and then the effect of HA molecular weight on the skin permeability of EA was explored to clarify the optimal molecular weight of HA with best transdermal delivery effectiveness. Finally, a series of in vitro and in vivo experiments were conducted to survey the transdermal mechanism, skin irritation, antioxidant, anti-photo ageing and antiwrinkle effects of EA-loaded liposomes modified with the optimal molecular weight of HA. The results showed that EA-HA-L had less than 200 nm particle size and high encapsulation efficiency.

Rapid detection of hydrogen sulfide utilizing dinitrophenyl ether-based fluorescent probes incorporating aldehyde functional groups.

Hydrogen sulfide (HS) plays a crucial role in the regulation of various physiological processes within living organisms, including cell signaling, vascular tone modulation, and the inflammatory response. The real-time detection and visualization of HS levels in practical samples hold significant importance for biological analysis. In this study, 3-(benzo[d]thiazol-2-yl)-5-bromo-2-(2,4-dinitrophenoxy)benzaldehyde (DTBH) was designed and synthesized based on the mechanism of excited state intramolecular proton transfer (ESIPT).

Development of an extractive spectrophotometric method for the determination of spermidine in urine samples using cucurbit[7]uril-truxene derivative as a fluorescent extractant.

Spermidine has emerged as a promising biomarker for the early-stage diagnosis of cancer in humans. A novel extractive spectrophotometric analytical method has been developed for the determination of spermidine in urine samples. This technique leverages the selective extraction of spermidine from complex matrices using a cucurbit[7]uril-truxene derivative (Q[7]-Cs-Tr) as a fluorescent extractant.

Association between novel inflammatory biomarkers SII, SIRI, and obesity in sedentary adults: NHANES 2007-2020.

Sedentary behavior is prevalent in modern society and is closely associated with various chronic diseases, including obesity. The Systemic Immune-Inflammation Index (SII) and the Systemic Inflammation Response Index (SIRI), as novel inflammatory biomarkers, may play an important role in predicting obesity risk. This study utilized data from the 2007-2020 National Health and Nutrition Examination Survey (NHANES) to analyze the relationship between sedentary behavior, SII, SIRI, and obesity through multivariable logistic regression models.

Alpha Ketoglutaric Acid attenuates LPS Induced Inflammatory Response by Inhibiting the PKCε/MAPK/P65 Signaling Pathway and Inhibit Oxidative Stress in Kupffer Cells.

The inflammatory response is crucial in the progression of various liver diseases. Many diseases are linked with inflammation, such as ischemia and reperfusion injury, nonalcoholic steatohepatitis (NASH) and hepatic fibrosis. Research indicates that αKG attenuates NASH.

Distinct Airway Microbiome and Metabolite Profiles in Eosinophilic and Neutrophilic Asthma.

Background: Asthma is a chronic, heterogeneous disease driven by inflammatory phenotypes, primarily eosinophilic asthma (EA) and neutrophilic asthma (NEA). While allergen triggers are well-known, the role of the airway microbiome and metabolites in asthma exacerbations remains poorly understood.

Methods: We recruited 64 participants (24 EA, 20 NEA, 20 healthy controls [HC]) for the discovery cohort, with validation in an external cohort (10 EA, 8 NEA, 8 HC).

Glymphatic Function Abnormality and Its Association With Pathophysiology in Primary Open-Angle Glaucoma.

Background: Primary open-angle glaucoma (POAG) has been increasingly viewed as a neurodegenerative condition, yet whether there are changes in the brain glymphatic function in patients with POAG has remained unclear. This study aimed to investigate the changes of glymphatic function in POAG based on multi-modal magnetic resonance imaging (MRI).

Methods: Thirty-eight patients with POAG and 25 age- and sex-matched healthy controls underwent structural MRI and functional MRI scans to assess abnormalities in cerebrospinal fluid circulation by choroid plexus (CP) volume and cortical glymphatic system clearance function by blood-oxygen-level-dependent signals and cerebrospinal fluid signals (BOLD-CSF) coupling.

NOC2L inhibits trophoblast ferroptosis in preeclampsia through the p53/SLC7A11 pathway.

Background: NOC2L is downregulated in preeclampsia (PE). However, the underlying functional mechanisms of NOC2L in the pathogenesis of PE remain unclear.

Methods: Cell viability, migration, and invasion were determined in hypoxia-stimulated HTR-8/SVneo cells in CCK-8, wound healing, and Transwell assays.

The role of KAT6A in regulating primordial follicle activation in mouse ovary.

Follicle is a primary structural and functional unit within the mammalian ovary, encompassing a centrally positioned oocyte surrounded by pregranulosa cells. Primordial follicles constitute the ovarian reserve, the activated primary follicle represents the inception of follicular development in mammals. The intricate balance between primordial follicle dormancy and activation is pivotal for sustaining ovarian reproductive function; over-activation of the primordial follicle pool can result in premature ovarian failure among women.

Metagenomics reveals combined effects of microplastics and antibiotics on microbial community structure and function in coastal sediments.

Microplastics and antibiotics are emerging pollutants in marine environments, yet their combined effects on coastal sediments remain poorly understood. This study examined the impacts of microplastics and antibiotics on sediment properties and microbial communities through a 60-day laboratory simulation. Results showed that microplastics significantly reduced carbon, nitrogen, and phosphorus levels in sediments, while both antibiotics and combined pollution decreased phosphorus content.

Phytodegradation and phytotoxicity of N-ethyl perfluorooctane sulfonamidoethanol (N-EtFOSE) in pakchoi (Brassica chinensis L.) via root and foliar uptake from physio-biochemical and transcriptomic perspectives.

N-ethyl perfluorooctane sulfonamidoethanol (N-EtFOSE), a known volatile perfluorooctane sulfonate (PFOS) precursor (PreFOS), has been widely detected in the environment. However, the mechanisms underlying the behaviors and phytotoxicity of N-EtFOSE in plants are unknown. Integrative physio-biochemical and transcriptomic analyses were used to reveal the accumulation, biotransformation, and toxicity of N-EtFOSE in pakchoi (Brassica chinensis L.

DDoS attack detection method based on improved convolutional long short-term memory and three-way decision in SDN.

Software Defined Networking (SDN) is an emerging network architecture and management method, whose core idea is to separate the network control plane from the data transmission plane. It is precisely because of this characteristic that SDN controllers are susceptible to external malicious attacks, the most common of which are Distributed Denial of Service (DDoS) attacks. This paper suggests a way to find DDoS attacks called ConvLTSM-MHA-TWD.

Analysis of the Independent Risk Factors of second-Degree Atrioventricular Block in Patients with Atrial Fibrillation and the Diagnostic Efficacy of Dynamic Electrocardiogram.

Objective: Exploring the independent risk factors of second-degree atrioventricular block (II AVB) in patients with atrial fibrillation (AF), and to evaluate the clinical value of 24-hour dynamic electrocardiogram (DCG) in its diagnosis.

Methods: A prospective cohort study was conducted on 947 patients with AF diagnosed and treated in our hospital from January 1, 2021 to December 31, 2021. These patients were divided into combined group (98 cases) and uncombined group (849 cases) according to whether they were accompanied by.

Does a growth mindset curb anxiety among Chinese medical students? Validation based on propensity score matching.

Background: Excessive anxiety is a common and hazardous mental disorder, especially in medical education. Positive psychology has proposed an effective path to reduce anxiety among medical students through mindset change. This study tests whether a growth mindset curbs anxiety in Chinese medical students.

Deep learning-enhanced hyperspectral imaging for rapid screening of Co-metabolic microplastic-degrading bacteria in environmental samples.

Microbial biodegradation of microplastic (MP) emerges as an environmentally benign and highly promising strategy for alleviating MP pollution in the ecosystem. Conventional approaches for screening MP-degrading bacteria use pollutants as the sole carbon source. Co-metabolism plays an essential role in microbial screening, as it enables the discovery of additional degrading microorganisms.

Establishment and optimization of a rapid and convenient viral RNA transcript copy reduction neutralization test (VcRNT) for quantification of hantaan orthohantavirus (HTNV) neutralizing antibodies.

Rodent-borne orthohantavirus causes severe hemorrhagic fever worldwide, with hemorrhagic fever with renal syndrome (HFRS) in Eurasia, and hantavirus cardiopulmonary syndrome (HCPS) in the Amrerica. In East Asia, Hantaan orthohantavirus (HTNV) is the main pathogen responsible for severe HFRS, with a case fatality rate up to 10 % with no specific treatment available. The antisera or neutralizing antibody (NAb) is able to block virus infection, however, the traditional NAb titer measuring based on focus reduction neutralization test (FRNT) is quite labour-extensive and takes 7-10 days.

Elucidation of the Ultrafast Origin of Multiphasic Dynamics in a Far-Red-Sensing Cyanobacteriochrome.

Cyanobacteriochromes are photoreceptors that constitute a significant subset of phycocyanobilin-bound proteins, yet the details of their excited-state photochemical and structural dynamics have not been fully elucidated. Here, we investigate the photoisomerization dynamics of a newly identified far-red/orange light-absorbing cyanobacteriochrome using femtosecond-resolved fluorescence and absorption methods. We observed active-site relaxations ranging from a few to hundreds of picoseconds for both far-red and orange-absorbing states.

Study of heat transfer model and buried thermocouple test of bell-type annealing furnace based on thermal equilibrium.

The bell-type annealing furnace is a key equipment in the cold rolling process, and the heat transfer process inside the furnace has a significant impact on the annealing process and product quality. At present, there are shortcomings in the calculation accuracy of model research based on heat transfer theory and practical experience. In addition, existing research has not fully considered the influence of gas flow distribution and corresponding heat transfer coefficient in each channel of multi tube steel coils on the temperature field.

Exploration of interaction mechanism and removal performance of polystyrene nanoplastics with covalent organic framework: Experimental and theoretical study.

Nanoplastics pollution in the environment has attracted heightened attention due to the potential for nanoplastics to adsorb and combine with other pollutants to increase their toxicity and endanger human health. Therefore, there is an urgent demand for effective strategies to remediate nanoplastics contamination in aquatic systems. In this study, COF was experimentally used to remove nanoplastics for the first time.