Biotransformation of 22-hydroxy-23,24-bisnorchol-4-ene-3-one by Gibberella fujikuroi CICC 40272-A to discover novel C22 steroid derivatives.

The microbial transformation of steroids offers a sustainable and selective approach to synthesize pharmacologically valuable derivatives. This study investigated the biotransformation of 22-hydroxy-23,24-bisnorchol-4-ene-3-one (4-HBC) by Gibberella fujikuroi CICC 40272-A to produce novel C22 steroid derivatives. Among seven screened fungal strains, G.

Evaluation of Grouting Reinforcement Effectiveness in Coal Seam Floors Using the Monte Carlo Analytical Hierarchy Process: A Case Study of the Rongkang Coal Mine.

Pregrouting reinforcement of the coal seam floor is an effective measure for preventing floor water hazards. However, how to objectively evaluate the grouting effectiveness using data from drilling and grouting construction processes remains an urgent issue that needs to be addressed. Based on the coal seam floor grouting project at the Rongkang Coal Mine in Shanxi Province, this study developed a comprehensive evaluation index system for assessing the effectiveness of coal seam floor grouting, consisting of six evaluation indicators.

Targeting Nav1.5 with DKK678 attenuates NF-κB-driven inflammatory injury in skeletal muscle.

Skeletal muscle inflammation associated with sepsis has been identified as a critical pathological process contributing to metabolic dysfunction and poor clinical outcomes. Voltage-gated sodium channels (VGSCs) have been implicated in immune modulation; however, their specific roles in skeletal muscle inflammation remain poorly understood. In this study, the VGSC subtype Nav1.

Computational and Experimental Engineering of -Anethole Oxygenase for Enhancing Activity in Industrial Vanillin Biosynthesis.

Vanillin, a widely used aromatic compound, can be biocatalytically synthesized using -anethole oxygenase (TAO). However, wild-type TAO shows low activity and stability. This study utilized AlphaFold2/3 modeling, molecular docking, and MD simulations to identify key residues (R86, T90, and H118) involved in substrate binding and catalysis.

Support measures and demand among healthcare workers during the COVID-19 pandemic: a survey in high-workload provinces of China, 2023.

Objectives: To assess the demands for support measures of healthcare workers (HCWs) in China's high-workload provinces during the COVID-19 pandemic and identify specific needs to make evidence-based recommendations.

Design: Prospective cross-sectional study.

Setting: Conducted in three Chinese provinces characterised by their significant healthcare demands during the pandemic.

A tungsten polyoxometalate mediated aqueous redox flow battery with high open-circuit voltage up to 2 V.

As a promising stationary energy storage device, aqueous redox flow battery (ARFB) still faces the challenge of low open-circuit voltage, due to the limitation of the potential of water splitting (1.23 V theoretically). Herein, we present a low potential anolyte design by using Na substituted phosphotungstic acid (3Na-PW) for an aqueous redox flow battery with the high open-circuit voltage up to 2.

Baicalein as a potent antifungal agent against : synergy with fluconazole and sustainable production through probiotic-mediated bioconversion.

Fungal infections, particularly those caused by , represent a significant global health concern, with drug resistance and biofilm formation posing considerable challenges to effective treatment. Baicalein, a flavonoid derived from baicalin found in , has demonstrated considerable antifungal efficacy. Moreover, the combination of baicalein and fluconazole demonstrated a notable synergistic effect.

Antimicrobial activity of octyl gallate nanoemulsion combined with photodynamic technology and its effect on food preservation.

Photodynamic inactivation, as a safe and effective antimicrobial technology that does not damage the organoleptic properties of the food itself, decreases the use of preservatives and is gradually gaining attention in the food industry. This study selected octyl gallate (OG) as an antimicrobial photosensitizer with eucalyptus oil as the oil phase and prepared it as an octyl gallate nanoemulsion (OG-NE) to ensure the delivery of the photosensitizer. Escherichia coli and Staphylococcus aureus inactivation with the OG-NE combined with photodynamic technology, as well as the effect on the quality of food products, was investigated.

An interaction study of online learning satisfaction with parent-child relationships and trait coping styles.

As the physical and mental development of the young is not only influenced by the parent-child relationship (PR) and the student's academic performance, but also moderated by trait coping styles (TCS), the changes between these three during the online learning period in an epidemic need to be reconsidered. This study aims to explore the factors affecting online learning satisfaction (OLS) among students and their interaction with parent-child relationship and trait coping style. A web-based questionnaire was employed, encompassing general information, the Trait Coping Style Questionnaire (TCSQ), and queries related to OLS.

Coassembly Nanomedicine Mediated by Intermolecular Interactions Between Methotrexate and Baricitinib for Improved Rheumatoid Arthritis Treatment.

The combination of anti-rheumatoid arthritis (RA) drugs methotrexate (MTX) and baricitinib (BTN) has been reported to improve RA treatment efficacy. However, study on the strategy of combination is elusive when considering the benefit of the synergy between MTX and BTN. In this study, we found that the N-heterocyclic rings in the MTX and BTN offer hydrogen bonds and π-π stacking interactions, driving the formation of exquisite vesicular morphology of nanovesicles, denoted as MB NVs.

1,5,6-Trimethoxy-2,7-dihydroxyphenanthrene from Exhibited Antitumor Activities for HeLa Cells.

Natural products are irreplaceable reservoirs for cancer treatments. In this study, 12 phenanthrene compounds were extracted and isolated from . Each chemical structure was identified using comprehensive NMR analysis.

Biosynthesis of Vanillin by Rational Design of Enoyl-CoA Hydratase/Lyase.

Vanillin holds significant importance as a flavoring agent in various industries, including food, pharmaceuticals, and cosmetics. The CoA-dependent pathway for the biosynthesis of vanillin from ferulic acid involved feruloyl-CoA synthase (Fcs) and enoyl-CoA hydratase/lyase (Ech). In this research, the Fcs and Ech were derived from sp.

Exploring the Pivotal Components Influencing the Side Effects Induced by an Analgesic-Antitumor Peptide from Scorpion Venom on Human Voltage-Gated Sodium Channels 1.4 and 1.5 through Computational Simulation.

Voltage-gated sodium channels (VGSCs, or Na) are important determinants of action potential generation and propagation. Efforts are underway to develop medicines targeting different channel subtypes for the treatment of related channelopathies. However, a high degree of conservation across its nine subtypes could lead to the off-target adverse effects on skeletal and cardiac muscles due to acting on primary skeletal muscle sodium channel Na1.

A sensitive and rapid immunoassay for Mycoplasma pneumoniae in children with pneumonia based on single-walled carbon nanotubes.

Mycoplasma pneumoniae(MP) is a leading pathogen of respiratory infection, especially community-acquired pneumonia (CAP), in children worldwide. However, its diagnosis is frequently ineffective because bacterial culture and serology test are usually positive 1-2 weeks or more after the disease onset. To achieve a better detection efficiency, the single-walled carbon nanotubes(SWCNT) were coupled with the colloidal gold-monoclonal antibody immunochromatographic strips(CGIC).

Screening for potential serum-based proteomic biomarkers for human type 2 diabetes mellitus using MALDI-TOF MS.

Background: Type 2 diabetes mellitus (T2DM) is a complex, pandemic disease contributing towards the global burden of health issues. To date, there are no simple clinical tests for the early detection of T2DM.

Method: To identify potential peptide biomarkers for such applications, 406 sera of T2DM patients (n = 206) and healthy controls (n = 200) are analyzed by using MALDI-TOF MS with a cross-sectional case-control design.

The isolation and identification of a light-induced protein in alfalfa sprouts and the cloning of its specific promoter.

We used 2D-PAGE to isolate a light-induced protein (AL-A) that is expressed abundantly in light-growth alfalfa sprouts. The seven amino acids of the N-terminal region of the protein were identified, and we searched for the protein in GenBank using the BLAST program. The results of the homology analysis showed that the amino acid sequence of the isolated protein is most similar to one from a pea plastocyanin.

Therapeutic effects of combination of paeoniflorin and albiflorin from Paeonia radix on radiation and chemotherapy-induced myelosuppression in mice and rabbits.

The aim of this study was to investigate the therapeutic effects of the combination of paeoniflorin and albiflorin (CPA) extracted from Paeonia radix on radiation and chemotherapy induced myelosuppression in two animal models: mice and rabbits. Mice were exposed to X-ray radiation (400 Roentgen), and both mice and rabbits were intraperitoneally injected with cyclophosphamide (100.0 mg/kg) and cytarabine chloride (92.

Identification and characterization of three impurities in clocortolone pivalate.

Clocortolone pivalate is a synthetic corticosteroid that can be used to cure corticosteroid-responsive dermatoses. Three previously unknown impurities detected by HPLC were isolated by semi-preparative LC. Based on the NMR and MS spectral data, these were identified as (6R,9R,16R)-9-chloro-6β-fluoro-11β,21-dihydroxy-16α-methylpregna-1,4-diene-3,20-dione-21-pivalate (Impurity I), (9R,16R)-9-chloro-4-fluoro-11β,21-dihydroxy-16α-methylpregna-1,4-diene-3,20-dione-21-pivalate (Impurity II) and (9R,16R)-9-chloro-6α-fluoro-11β,21-dihydroxy-16α-methylpregna-1,4-diene-3,20-dione-11,21-dipivalate (Impurity III).

Studies on the stability of salvianolic acid B as potential drug material.

Introduction: Salvianolic acid B (Sal B) is one of the major water-soluble compounds isolated from the roots of Salvia miltiorrhiza, which is widely used as a traditional Chinese medicine. Although much research on the general stability of Sal B has been undertaken and reported, there is still a need for further study of the stability required as a potential drug material.

Objective: To study the stability of Sal B in the solid state and in normal saline (NS) solution during storage, as required in the ICH guidelines (2003) and Chinese Pharmacopoeia (2005).

Stereoselective hydrogenation on the exocyclic and conjugated double bond of sesquiterpene lactones by Aspergillus versicolor D-1.

Aspergillus versicolor D-1 was employed to convert dehydrocostuslactone (1) and 3-hydroxy-1(10),3,11(13)-guaiatriene-12,6-olide-2-one (5) stereoselectively. The reactions occurring were specific hydrogenation on the exocyclic alpha,beta-double bond of sesquiterpene lactones with excellent conversion. Products were identified by the analysis of their spectra such as UV, IR, MS, (1)H, (13)C NMR, and NOESY, and the structure of one new compound was elucidated.

DNA methylation changes in cell line from beta-lactoglobulin gene targeted fetus.

The gene targeting combined somatic cell nuclear transfer is very useful in agriculture and medicine. Epigenetic modification of DNA by methylation is significant in regulating gene expression during mammalian development. During gene targeting, epigenetic status of donor cell nuclei may be changed in a series of processes, including homologous recombination, cell selection and cloning.

[Research progress and prospect of animal gene targeting].

Established upon the embryo stem cell technique and homologous recombination, gene targeting has been widely used in the genome specific manipulation, especially applied in the genetic trait modification of transgenic animal. Our paper reviewed the history of transgenic animal, somatic cloning and gene targeting, which indicates the influence towards the current status and prospect of the transgenic animal production.

[The factors affecting the efficiency of mutiplex PCR].

In the research,the outputs of different cycle parameters, PCR buffer and reaction volumes are compared. The results indicated that the annealing temperature, annealing time, elongated time and ingredient of PCR buffer affected mutiplex PCR, but the reaction volume and cycle number had few effect on it.

[Preliminary analysis on China Agricultural University chicken resource population based on genomic scanning].

Combining the technique of multiplex-PCR and the fluorescent semi-automated detection, a large-scale genome scanning was performed for 440 chickens, which was derived from China Agricultural University chicken resource families, within three generations. Fifty-five microsatellite markers were analyzed for this study. Those 55 microsatellite loci accorded with the characters of Mendelian co-inheritance.

Construction and characterization of a novel 13.34-fold chicken bacterial artificial chromosome library.

A chicken bacterial artificial chromosome (BAC) library consisting of 138,240 clones was constructed in vector pBeloBAC11 with genomic DNA isolated from female white-silk chicken. An average insert size of 118 kb was estimated from 452 randomly isolated clones, which indicate the library to be approximate 13.34-fold genome coverage.