A flexible microneedle sensor for long-term, enzyme-free glucose monitoring with high sensitivity and stability.

Background: Blood glucose monitoring is of paramount importance for patients with diabetes. However, conventional glucose meters exhibit limited accuracy and present challenges for prolonged use, primarily due to the high consumption of glucose oxidase (GOD) and their sensitivity to temperature and environmental factors. phenylboronic acid (PBA), benefiting from its reversible reaction with glucose and superior stability, holds potential as a promising medium for long-term glucose monitoring.

Self-healing Cu single-atom catalyst for high-performance electrocatalytic CO methanation.

To address the escalating challenge of atmospheric CO emissions, this study proposes a self-healing Cu single atom (SA) catalyst design. By partially cleaving Cu-N bonds via hydrogen evolution reaction (HER), coordinatively unsaturated Cu sites form and spontaneously bond with adjacent ZrO clusters which are strategically positioned near the Cu SA, creating a hybrid Cu-N/O structure with enhanced performance. In situ Raman and X-ray absorption fine structure (XAFS) measurements confirm the dynamic reconstruction of coordination environment from CuN to CuNO under electrochemical conditions.

Remote Sensing Image Generation via Object Text Decoupling.

Remote sensing images usually reveal various objects with complex structures and different locations within vast ground area backgrounds. That leads to a major challenge for conventional generative models in handling remote sensing objects with correct shapes and clear textures. Integrating additional object-level controls can be a potential solution to improve generation quality, yet previous approaches inject the object-related conditions by specifying their locations, causing a limitation in object layout in generated results.

[Application of animal drugs in uro-andrological diseases under the guidance of diversified theories of traditional Chinese medicine].

Animal drugs have been used in the treatment of uro-andrological diseases since ancient times. At present, the clinical use of animal drugs is mostly based on the experience of doctors. From the perspective of the diversified theories of traditional Chinese medicine (TCM), this paper summarizes the experience of predecessors in the use of animal drugs, and believes that under the guidance of the TCM theories, the application of animal drugs in the treatment of uro-andrological diseases has achieved multi-dimensional and diversified development, as in the treatment of chronic prostatitis based on the theories of "heat poison", "stasis poison" and "aromatherapy through the orifice", and in the management of ED from the perspective of "kidney deficiency", "qi-blood disharmony" and "collateral disease", which accords with the mainstream thought of current scholars in the differentiation and treatment of uro-andrological diseases.

Evolving trends in HPV vaccination coverage among women aged 9-45 in Chengdu, China: insights from 2017 to 2023.

Background: Chengdu, China, is facing an increasing burden of cervical cancer. Although human papillomavirus (HPV) vaccines have been introduced in China since 2016, vaccination coverage remains suboptimal, and data on regional disparities are limited. In 2021, Chengdu implemented a subsidized HPV vaccination program targeting girls aged 13-14 years.

Utilizing multiplex reverse transcription-multiple cross displacement amplification-lateral flow biosensor technology for detecting H1N1, H3N2 and H7N9 influenza A virus subtypes.

Respiratory infections caused by influenza A viruses (IAVs) pose a global threat annually, leading to significant mortality in severe cases. IAVs are classified into various subtypes, and a combination of multiplex reverse transcription-multiple cross displacement amplification (mRT-MCDA) and lateral flow biosensor (LFB) has been developed to detect three subtypes (H1N1, H3N2, and H7N9) that have been frequently observed in recent years. This technology simultaneously reverse transcribes and amplifies two target genes, hemagglutinin (HA) and neuraminidase (NA), at a constant temperature of 63 °C in just 40 min.

Cucurbitacin B alleviates DSS-induced ulcerative colitis by improving gut microbiota disorder in C57BL/6 mice.

Cucurbitacin B (CuB) is a triterpenoid compound derived from various medicinal plants, demonstrating potential anti-inflammatory, antioxidant, and neuroprotective properties, as well as significant anti-tumor effects. However, its efficacy in treating ulcerative colitis (UC) remains unclear. To investigate the therapeutic potential of CuB, a dextran sulfate sodium (DSS)-induced UC model in mice was employed, along with gut microbiota analysis.

Clinical value of transperineal prostate biopsy guided by cognitive fusion of magnetic resonance and transrectal ultrasound.

This study assesses the clinical value of magnetic resonance imaging (MRI) and transrectal ultrasonography (TRUS) cognitive fusion-guided transperineal prostate biopsy for prostate cancer detection. A total of 110 patients with suspected prostate cancer admitted from June 2019 to June 2021 were included. All patients underwent systematic TRUS-guided biopsy and MRI-TRUS cognitive fusion-guided transperineal biopsy.

Rose-Mamba-YOLO: an enhanced framework for efficient and accurate greenhouse rose monitoring.

Accurately detecting roses in UAV-captured greenhouse imagery presents significant challenges due to occlusions, scale variability, and complex environmental conditions. To address these issues, this study introduces ROSE-MAMBA-YOLO, a hybrid detection framework that combines the efficiency of YOLOv11 with Mamba-inspired state-space modeling to enhance feature extraction, multi-scale fusion, and contextual representation. The model achieves a mAP@50 of 87.

A novel data-driven approach to assess the chewability and grittiness of the chewable tablets using a texture analysis.

Context: Chewable tablet palatability significantly impacts patient compliance, but current pharmacopeias have no standardized evaluation methods. This requires developing an objective system for assessing the palatability of chewable dosage forms, which will aid in setting quality control standards.

Objective: Using a texture analyzer, this study aimed to develop an objective, data-driven approach to evaluate chewability and grittiness in chewable tablets.

From traditional medicine to targeted therapy: Structure-activity relationship-guided optimization of scorpion toxin DKK2 for pain-associated sodium channel blockade.

Ethnopharmacological Relevance: Scorpion toxins, as critical bioactive components in traditional Chinese medicine (TCM), show potential as non-addictive analgesics targeting the pain-associated sodium channels (Na1.7, Na1.8, and Na1.

Glycyrrhizic Acid From Glycyrrhiza uralensis Fish: A Novel Therapeutic Candidate for Alzheimer's Disease Targeting the JNK Signaling Pathway.

Alzheimer's disease (AD) has emerged as a prevalent public health concern. This study aims to investigate the mechanism of Glycyrrhiza uralensis Fish. in AD through the utilization of network pharmacology, molecular docking, and in vitro validation techniques.

Tracing and Capturing the Epiblast Pluripotency of Sheep Preimplantation Embryos.

Capturing different pluripotent state stem cells from epiblast in vitro helps understand embryonic development and provides invaluable cell sources for basic research and regenerative medicine. Sheep are not only one of the most important livestock species in agriculture but also serve as an ideal preclinical model for studying human disease. Single-cell transcriptome analysis of sheep preimplantation embryos from embryonic day (E) 1 to E14 is performed to investigate the pluripotency changes of epiblast and elucidate the pluripotent regulation signaling.

Glomerular mesangial derived extracellular vesicles deteriorate diabetic kidney disease via miR-3147/PRKAR2B axis.

To investigate the clinical significance and role of miRNAs with shared patterns in both peripheral blood and kidney tissue-derived extracellular vesicles (EVs) in diabetic kidney disease (DKD). miRNA-Seq was performed on plasma EV samples from DKD and diabetes mellitus (DM) patients, and the validation, clinical significance and mechanistic exploration of the specific differentially expressed miRNAs (De-miRNAs) were evaluated in a cohort of DKD-derived plasma and glomerular mesangial biopsies and high glucose (HG)-treated mesangial cells (MCs). A total of 15 EV-derived De-miRNAs was identified by miRNA-Seq, among which miR-3147 was the most significantly differentially expressed.

Enhanced hermit crabs detection using super-resolution reconstruction and improved YOLOv8 on UAV-captured imagery.

Hermit crabs are vital to coastal ecosystems, serving as environmental health indicators and contributing to seed dispersal, debris cleanup, and soil disturbance. Traditional hermit crabs survey methods, like quadrat sampling, are labor-intensive and environmentally dependent. This study presents an innovative approach combining UAV (Unmanned Aerial Vehicles)-based remote sensing with Super-Resolution Reconstruction (SRR) and the CRAB-YOLO detection network, a modification of YOLOv8s, to monitor hermit crabs effectively.

Computational and Experimental Engineering of -Anethole Oxygenase for Enhancing Activity in Industrial Vanillin Biosynthesis.

Vanillin, a widely used aromatic compound, can be biocatalytically synthesized using -anethole oxygenase (TAO). However, wild-type TAO shows low activity and stability. This study utilized AlphaFold2/3 modeling, molecular docking, and MD simulations to identify key residues (R86, T90, and H118) involved in substrate binding and catalysis.

Comparative Control of : Growth-Inhibiting Effects of Chemical Insecticides Versus the Green Advantages of a Biopesticide.

is a major pest of , with chemical pesticides being the primary method for pest control. However, their negative impact on crop growth and environmental risks have prompted the search for low-toxicity, environmentally friendly alternatives. This study systematically evaluates the control effectiveness of two commonly used chemical pesticides, Zu Jia and Jie Tiao, along with their impact on the growth characteristics of and compares them to the biopesticide (Bt) strain G033A.

Arbutin ameliorated depression by inhibiting neuroinflammation and modulating intestinal flora.

Background: Arbutin (AR), a natural glycoside compound widely distributed in botanical sources, exerts neuroprotective properties. Nevertheless, the pharmacological effect of AR on depression and its underlying mechanism remain undefined.

Methods: The mice were induced by chronic unpredictable mild stress (CUMS) procedure 16S rRNA sequencing and metabolomic analyses were conducted.

Postoperative reintubation as risk factor for anastomotic stricture after congenital esophageal atresia.

Objective: To examine the consequences of preoperative invasive ventilation (IV), postoperative IV and reintubation on the occurrence of ES in type C CEA patients.

Methods: This retrospective study collected the data from patients diagnosed as Gross type C CEA and underwent surgical repair at our institution between January 2013 and December 2022. Comprehensive data, including demographic characteristics, preoperative comorbidities, perioperative indicators, and postoperative outcomes were collected and analyzed.

Multistage nucleation pathway in LiF molten salt mirrors the crystal-melt interface structure.

Despite over a century of studies, fundamental questions remain about the processes governing crystal nucleation from melts or solutions. Research over the past three decades has presented mounting evidence for kinetic pathways of crystal nucleation that are more complex than envisioned by the simplest forms of classical theory. Such observations have been presented for colloidal and elemental systems with covalent and metallic bonding.

Long-term investigation of 3D tissue detailed structure and multiparametric vascular network properties using OCT/OCTA for guiding atopic dermatitis theranostics.

Background: Rapid, label-free and depth-resolved imaging of atopic dermatitis (AD) at high resolution has long been a challenge due to current dermoscopes are limited by inadequate resolution, sensitivity and the lack of three-dimensional (3D) imaging capability.

Methods: Herein, we present promising optical coherence tomography (OCT) and OCT angiography (OCTA) collaborative multi-parameter image processing algorithms (OCT/OCTA) that have been developed for long-term, dynamic longitudinal investigation of subtly changes in tissue structure during the dynamic development of AD, especially in the microvascular network.

Results: The OCT image demonstrated a 'roller-coaster' trend in skin thickness throughout the progression of dermatitis, initially exhibiting an increase and subsequently a decrease.

HECW2 knockdown suppresses the development of infantile hemangioma by inhibiting ALKBH5/LDHA axis-mediated glycolysis.

HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), a member of E3 ubiquitin ligase family, was identified as a hub gene in infantile hemangioma (IH). This study investigated the roles and mechanisms of HECW2 in IH development. Our investigation revealed that HECW2 was up-regulated in proliferative and involuting IH tissues compared with normal adjacent tissues.

In-situ Raman spectroscopy for soft contacts.

Hypothesis: Contacting surfaces often experience deformations across multiple length scales. These deformations become particularly complex when one of the materials is soft, such as elastomers.Elastomers are widely used in flexible electronics and intelligent interactive systems, where their surface deformability facilitates the acquisition of multimodal physical information.

Melittin-Loaded Nanoparticle Microneedles Targeting M1 Macrophage for Arthritis Treatment.

Arthritis is a joint inflammatory disease with multiple types that significantly compromises patients' quality of life. Current therapeutic efficiency is frequently constrained by the necessity for high dosages, the requirement for frequent administration, substantial side effects, and the risk of drug resistance. New therapeutic strategies are urgently needed.

Multifunctional Cd(II) Metal-Organic Framework with Abundant Lewis Acidic and Basic Sites: Selective Gas Adsorption and Separation, CO Catalytic Fixation, and Fluorescence Recognition of Uric Acid.

The development of new porous materials for the selective adsorption and fixation of CO, as well as the selective capture of CH, is essential for environmental protection and energy security. Herein, a versatile coordination polymer, {[Cd(btbpa)(HO)]·3HO·4NMP·DMA} (, Hbtbpa = 4,4'-bis(1-1,2,4-triazole-1-yl)-[1,1'-biphenyl]-3,3'-dicarboxylic acid), has been prepared, which not only exhibits good chemical and thermal stability but also possesses adaptive nanochannels. Gas uptake studies disclosed the selective adsorption properties of MOF for CO and CH over other gases (N, CH, CH, and CH), contributing to a record-high IAST selectivity of ca.