Enhancing the antifungal efficiency of chitosan nanoparticle via interacting with didymin/flavonoid and its bio-based approaches for postharvest preservation in pear fruit models.

In this study, chitosan nanoparticles are used to encapsulate didymin and flavonoids separately using ionic gelation with phytic acid as a cross-linker. Their structural, antioxidant, and antifungal properties were evaluated. Flavonoid (Fs) was extracted from orange peels, while didymin (Did) was qualified in the pure extract using ultra-performance liquid chromatography (UPLC).

Endoglucanase H from Plays an Important Role in the Virulence on Pear Fruits.

can infect many agricultural products including cereals, grapes, and pear. Pathogenic fungi secrete diverse effectors as invasive weapons for successful invasion the host plant. During the pathogen-host interaction, 4486 differentially expressed genes were observed in with 2773 up-regulated and 1713 down-regulated, whereas 8456 differentially expressed genes were detected in pear fruits with 4777 up-regulated and 3679 down-regulated.

Chitosan inhibits Penicillium expansum possibly by binding to DNA and triggering apoptosis.

Chitosan is a natural polysaccharide that is abundant, biocompatible and exhibits effective antifungal activity against various pathogenic fungi. However, the potential intracellular targets of chitosan in pathogenic fungi and the way of activity of chitosan are far from well known. The present work demonstrated that chitosan could inhibit Penicillium expansum, the principal causal agent of postharvest blue mold decay on apple fruits, by binding to DNA and triggering apoptosis.

Evaluation for the effect of low-carbon city pilot policy: evidence from industry in China.

The investigation of the implementation effect of the low-carbon city pilot (LCCP) policy in promoting industrial low-carbon development is important for economic growth and carbon reduction in China. This paper takes the industry sector as an independent investigation object, and aims to examine the effectiveness of the policy in improving industrial low-carbon development. The industrial total factor carbon emission efficiency index (ITFCEE) is constructed to measure the level of industrial low-carbon development by the global frontier directional distance function.

Heterologous expression and characterization of an M4 family extracellular metalloprotease for detergent application.

Proteolytic enzymes stand out as the most widely employed category utilized in manufacturing industry. A new protease was separated from Planococcus sp.11815 strain and named as nprS-15615 in this research.

Spatio-temporal evolution characteristics of eco-efficiency in the Yellow River Basin of China based on the super-efficient SBM model.

It is of great significance to study the trends and internal differences of eco-efficiency in the Yellow River Basin for ecological protection and high-quality development of the Yellow River Basin. According to the characteristics of the Yellow River Basin in China, the eco-efficiency evaluation system was constructed, and the super-efficiency slack-based measure (SBM) model and the super-efficiency SBM model of undesired output were used to calculate the eco-efficiency levels of provinces in the Yellow River Basin from 2005 to 2020, and the variation trend and internal differences were analyzed. The results show that when only the expected output was considered, the eco-efficiency of the Yellow River Basin as a whole and each province showed a fluctuating upward trend, but there were obvious differences.

Transcriptome Analysis and Functional Characterization Reveal That Gene Contributes to the Virulence of on Apple Fruits.

is the causal agent of blue mold decay on apple fruits and is also known to be the major producer of patulin, a mycotoxin that represents serious hazard to human health. Several mechanisms have been suggested to explain the pathogenesis of in host plants. Secreted effector proteins are vital for the pathogenicity of many fungal pathogens through manipulating their hosts for efficient colonization.

Analysis of urban carbon emission efficiency and influencing factors in the Yellow River Basin.

The Yellow River Basin is an energy-rich area. The low-carbon development of the Yellow River Basin is one of the ways to achieve ecological protection and high-quality development. Based on panel data from 2007 to 2019, the dynamic and static carbon emission efficiencies of 98 cities in the Yellow River Basin were calculated using the Undesirable Super Slacks-based model and the Global Malmquist-Luengerber index method, respectively, and the spatial effects of influencing factors were analyzed using the spatial Dubin model.

Characterization of Bacillus velezensis E2 with abilities to degrade ochratoxin A and biocontrol against Aspergillus westerdijkiae fc-1.

Ochratoxin A (OTA), primarily produced by the fungi belonging to the species of Aspergillus and Penicillium, is one of the most common mycotoxins found in cereals and fruits. In addition to resulting in huge economic losses, OTA contamination also poses considerable threat to human and livestock health. Microbial degradation of mycotoxins has been considered with great potential in mycotoxins decontamination.

Drivers of the peaking and decoupling between CO emissions and economic growth around 2030 in China.

Reaching the peak of carbon dioxide emissions is the basis and premise of carbon neutrality. In this paper, the factor decomposition model was used to analyze the influencing factors and effects of carbon dioxide emissions. Causal chain model of elastic decoupling was established.

LncRNA-SNHG16 promotes proliferation and migration of acute myeloid leukemia cells via PTEN/PI3K/AKT axis through suppressing CELF2 protein.

The silence of lncRNA small nucleolar RNA host gene 16 suppressed acute lymphoblastic leukemia (ALL) cell proliferation and migration, whereas its role in acute myeloid leukemia (AML) still lacks clarity. This study showed that was upregulated in AML patients and cells. And SNHG16 overexpression remarkably enhanced the proliferation and migration capacities of HL60 and AML-193 cells, while SNHG16 knockdown acted the opposite way.

Clinical features and treatment outcomes of primary ocular adnexal mucosa-associated lymphoid tissue lymphoma: a single center retrospective analysis of 64 patients in China.

Aim: To investigate the clinical features, treatment and prognosis of primary ocular adnexal mucosa-associated lymphoid tissue lymphoma (POAML).

Methods: A retrospective analysis was performed on 64 patients with POAML who were admitted to the First Affiliated Hospital of Zhengzhou University from January 2006 to December 2018.

Results: With a median follow-up of 61mo (range, 2-156mo), estimated overall survival (OS) rate and progression-free survival (PFS) rate at 10y reached 94.

Chlorogenic acid relieved oxidative stress injury in retinal ganglion cells through IncRNA-TUG1/Nrf2.

: To discover the possible underlying mechanism of Chlorogenic acid (CGA) in protecting against oxidative stress injury in glaucoma. : LncRNA TUG1 and Nrf2 expressions were detected by qRT-PCR and Western blot. Retinal ganglion cell (RGC) viability and apoptosis were measured by MTT and flow cytometry, respectively.

miR-221 promotes lens epithelial cells apoptosis through interacting with SIRT1 and E2F3.

MicroRNAs (miRNAs) have been regarded as potential modulators in varying ocular diseases, including age-related cataract (ARC). However, the roles of miR-221 in ARC progression and its underlying mechanism remain poorly understood. In this study, human lens epithelial cell line (SRA01/04) was used to investigate the potential function of miR-221 in vitro.

Depletion of MicroRNA-373 Represses the Replication of Hepatitis C Virus via Activation of Type 1 Interferon Response by Targeting IRF5.

Purpose: Hepatitis C virus (HCV) poses a risk of chronic liver disease and threatens a significant number of people worldwide. MicroRNAs (miRNAs) are linked to the regulation of hepatocarcinogenesis. Although miR-373 is required for HCV infection, the underlying mechanisms of miR-373 involvement in HCV replication remain elusive.

Conservation of orbital angular momentum for high harmonic generation of fractional vortex beams.

This work demonstrates conservation of average orbital angular momentum for high harmonic generation of fractional vortex beams. High harmonics are generated in reflected light beams in a three-dimensional particle-in-cell simulation. The average orbital angular momentum of the beam is calculated when a relativistic linearly polarized fractional vortex beam impinges on a solid foil.

LncRNA MALAT1 promotes the apoptosis and oxidative stress of human lens epithelial cells via p38MAPK pathway in diabetic cataract.

Background: LncRNAs are involved in various biological processes and disorders. We aimed to investigate the role of lncRNA MALAT1 deregulation in the pathogenic mechanism of diabetic cataract (DC).

Methods: The expression of MALAT1 in the tissues and cells was detected by qRT-PCR.

Effects of longitudinal body mass index variability on microvasculature over 5 years in adult Chinese.

Objective: To explore the associations of 5-year trend and fluctuation in body mass index (BMI) with retinal vascular caliber in a middle-aged and elderly Chinese population.

Methods: Participants age ≥40 years were recruited in a prospective study. Baseline BMI data were collected in 2008, and the participants were re-examined annually until 2012.

Association between body composition and retinal vascular caliber in children and adolescents.

Purpose: To elucidate the relationship between body composition and retinal vascular caliber (RVC) in children and adolescents.

Methods: Participants aged 7 to 19 years were recruited from the Guangzhou Twin Eye Study cohort. Retinal vascular caliber was measured cross-sectionally from retinal images using a computer-aided program.

Ethnicity-based subgroup meta-analysis of the association of LOXL1 polymorphisms with glaucoma.

Purpose: To investigate the association and ethnic heterogeneity of lysyl oxidase-like 1 (LOXL1) single nucleotide polymorphisms (SNPs) with exfoliation syndrome (XFS)/exfoliation glaucoma (XFG) and other types of glaucoma.

Methods: We performed meta-analysis and ethnicity-based subgroup analyses according to published studies. Allele and genotype frequencies of SNPs rs1048661, rs2165241, and rs3825942 were extracted for analysis in Reviewer Manager: (1) comparison of the allelic distributions between XFS and XFG, (2) allelic association of LOXL1 SNPs with XFS/XFG, (3) associations in homozygote, heterozygote, and dominant and recessive models, and (4) allelic association with primary open angle glaucoma (POAG).