[Occurrence characteristics of traditional Chinese medicine (TCM) root rot and prevention and control strategies against it under new situations].

Medicinal plant underground diseases, typified by root rot, directly result in a significant reduction in both the yield and quality of traditional Chinese medicine(TCM) because of its hidden occurrence and difficulty in prevention and control. Prevention and control measures depending on chemical pesticides bring potential risks to the safety of TCM and easily cause environmental pollution. The introduction of the new version of Good Agricultural Practice for Chinese Crude Drugs(GAP) and the enhancement of pesticide residue limit standards for TCM and decoction pieces in Chinese Pharmacopoeia(2025 edition) have elevated the requirements for green and efficient disease prevention and control technologies of TCM.

The m6A modification-mediated upregulation of ETS2 translation drives arsenic-induced spermatogonial senescence.

Accumulating evidence indicates that arsenic (As) exposure causes a decline in sperm quality. This study aimed to investigate the impact of As exposure on spermatogonial senescence. GC-1 cells were exposed to NaAsO (10 μM).

Isolation and Structural Characterization of Melanins from Red and Yellow Varieties of .

Melanin is a complex natural pigment that imparts a variety of colors to the fruiting bodies of edible fungi, influencing both their nutritional quality and commercial value. is an emerging type of edible fungus that has been widely cultivated in recent years. It can be categorized into red and yellow varieties based on cap color, while its pigment characteristics remain unclear.

Prediction of postoperative vault after implantable collamer lens implantation with deep learning.

Aim: To predict the post-operative vault and the suitable size of the implantable collamer lens (ICL) by comparing the performance of multiple artificial intelligence (AI) algorithms.

Methods: A retrospective analysis of 83 patients with 132 eyes was conducted from 2020 to 2023. All patients underwent implantation of EVO-V4C ICLs.

Evidence Mapping of Clinical Practice Guidelines Recommendations and Quality for Nutritional Management in Dementia.

Aim: This study aimed to systematically review Clinical Practice Guidelines (CPGs) for nutritional management of dementia and use evidence mapping to highlight research trends and identify gaps to inform future research.

Design: A systematic review of guidelines using the PRISMA statement.

Methods: Systematically collect literature on dementia management CPGs from PubMed, Embase, Web of Science and guideline databases.

High tidal volume mechanical ventilation exacerbates pulmonary injury via upregulation of PAI-1 expression in rats.

Objective: This study aimed to investigate the impact of different mechanical ventilation strategies on pulmonary plasminogen activator inhibitor-1 (PAI-1) expression in a rat model.

Methods: Seventy-two specific pathogen-free (SPF) adult male Sprague-Dawley (SD) rats were randomly assigned to four groups (n = 18): Group C (spontaneous breathing), group S (low tidal volume, VT = 6 mL/kg), group R (regular tidal volume, VT = 10 mL/kg), and group L (high tidal volume, VT = 40 mL/kg). Each group was further divided into three subgroups based on mechanical ventilation duration (2, 4, or 6 h).

One-pot synthesis of ferrocenyl-substituted allylamine derivatives by a three-component reaction of α-(hydroxy) alkyl ferrocenes, aldehydes and secondary amines.

We report an efficient one-pot, three-component synthesis of ferrocenyl-substituted allylamine derivatives with α-(hydroxy) alkyl ferrocenes, aldehydes and amines. The advantages of this method are its catalyst-free nature, broad substrate scope, operational simplicity and mild reaction conditions. The origin of this one-pot synthesis of ferrocenyl-substituted allylamine derivatives was explored through density functional theory calculations.

Correlation between death-coping self-efficacy and personality traits of intensive care unit nurses: a cross-sectional study.

Objective: This study aimed to explore the association between different personality traits and death-coping self-efficacy of intensive care unit (ICU) nurses.

Methods: A total of 350 questionnaires were distributed, and 301 valid questionnaires were finally collected. The research tools included a general information questionnaire and a death-coping self-efficacy questionnaire to collect nurses' demographic information.

Global perspectives on challenges, coping strategies, and future preparedness of nursing home staff during COVID-19: a systematic review and meta-synthesis.

Background: The global public health crisis caused by COVID-19 in late 2019 was unprecedented. Due to their vulnerable population, nursing homes are a key epidemic response area. This study described the challenges and coping strategies of nursing home staff during COVID-19 and proposed recommendations for future public health crises in nursing homes.

RSL1D1 regulates lung adenocarcinoma progression by inhibiting NRF2 ubiquitination.

RSL1D1, a member of the universal ribosomal protein uL1 family, plays a crucial role in various cellular processes. However, its involvement in cancer, particularly lung adenocarcinoma (LUAD), remains poorly understood. Our study reveals that RSL1D1 expression is significantly upregulated in LUAD and high levels of RSL1D1 are associated with poorer survival outcomes in LUAD patients.

Metallothioneins protect against isoconazole nitrate-induced antitumor activity through the maintenance of cellular redox homeostasis in hepatocellular carcinoma.

Hepatocellular carcinoma (HCC) is the predominant histological subtype of primary liver cancer with high cancer-related mortality. Despite the development of clinical advances, the prognosis of HCC patients remains very poor, and beneficial therapeutic strategies are urgently needed. Isoconazole nitrate (ISN) is an azole derivative that exhibits potent and broad-spectrum fungistatic activities for the clinical therapy of dermatomycosis.

Ophiopogonin C protects against acute lung injury by fatal sepsis through pyroptosis macrophage.

Background: Sepsis is a frequent complication of severe infection and trauma, and one of the common causes of acute lung injury (ALI). Macrophage pyroptosis plays an important role in sepsis-induced ALI, takes part in the regulation of the inflammatory response, and affects the damage and repair of lung tissue.

Purpose: This study attempts to reveal the protective mechanism of Ophiopogonin C against fatal sepsis induced ALI.

The impact of remimazolam sedation during neuraxial anesthesia on perioperative cognitive function in elderly patients: a multicenter randomized controlled study.

Background: Remimazolam, a novel ultra-short-acting benzodiazepine, is a potential sedative for non-general anesthesia surgery in the elderly. This study aimed to investigate the appropriate sedative dosage of remimazolam and its effects on perioperative cognitive function in elderly patients undergoing non-general anesthesia surgery.

Methods: This multicenter, placebo-controlled trial enrolled 330 elderly patients undergoing non-general anesthesia procedures at eight centers in China from July 2021 to February 2022, with 238 ultimately completing the study.

[Efficacy and mechanism of Guizhi Tongluo Tablets in alleviating atherosclerosis by inhibiting CD72hi macrophages].

This study investigates the effect and underlying mechanism of Guizhi Tongluo Tablets(GZTL) in treating atherosclerosis(AS) in a mouse model. Apolipoprotein E-knockout(ApoE~(-/-)) mice were randomly assigned to the following groups: model, high-, medium-, and low-dose GZTL, and atorvastatin(ATV), and age-matched C57BL/6J mice were selected as the control group. ApoE~(-/-) mice in other groups except the control group were fed with a high-fat diet for the modeling of AS and administrated with corresponding drugs via gavage for 8 weeks.

Metabolic reprogramming of tumor-associated neutrophils in tumor treatment and therapeutic resistance.

Tumor-associated neutrophils (TANs), pivotal immune cells within the tumor microenvironment (TME), exhibit dual potential in both pro- and anti-tumorigenic effects. These cells display remarkable heterogeneity and plasticity within the TME, adapting to hypoxic and nutrient-deprived conditions through metabolic reprogramming while critically influencing tumor progression, metastasis, and immune evasion. The metabolic reprogramming of TANs not only modulates their functional phenotypes but also reshapes tumor biological behaviors and therapeutic responses by regulating metabolic intermediates and cellular interactions within the TME.

miR-2400 promotes proliferation of bovine skeletal muscle-derived satellite cells by regulating MAGED1 genes expression.

microRNAs play a crucial role in the intricate process of muscle satellite cells proliferation and differentiation. Previous studies have demonstrated that miR-2400 can regulate bovine skeletal muscle satellite cell (MuSCs) proliferation, yet the underlying mechanism remains incompletely elucidated. In this study, we employed bioinformatics prediction and dual luciferase reporter assays to establish that miR-2400 directly targets the 3' untranslated regions (UTRs) of melanoma antigen family D1 (MAGED1) mRNA, thereby suppressing its expression.

Clinical efficacy and quality of life in elderly patients with lumbar degenerative disease treated with TLIF combined with unilateral pedicle screw fixation: a randomized controlled study.

Objective: To conduct a comparative analysis of the clinical efficacy and quality of life in elderly patients with lumbar degenerative disease (LDD) treated with TLIF (transforaminal lumbar interbody fusion) combined with either unilateral pedicle screw fixation (UPSF) or bilateral pedicle screw fixation (BPSF).

Methods: A total of 112 patients with single- or double-segment lumbar degenerative disease were divided into two groups. In the unilateral fixation cohort, 32 single-segment patients and 22 double-segment patients underwent TLIF combined with UPSF, whereas in the bilateral fixation cohort, 34 single-segment patients and 24 double-segment patients underwent TLIF combined with BPSF.

Author Correction: Lactylation-driven FTO targets CDK2 to aggravate microvascular anomalies in diabetic retinopathy.

[Image: see text]

Enantioselective Propargylation and Allenylation of Isatins Using Chiral Box-Copper/Zinc Catalysts.

A method for the asymmetric propargylation and allenylation of isatins catalyzed using chiral Box-copper/zinc catalysts is described. By modulation of the metal and solvent, a series of chiral 3-hydroxy-3-propargyl-2-oxindoles and 3-hydroxy-3-allenyl-2-oxindoles are selectively synthesized in good yields with excellent enantio- and regioselectivities.

Increasing Prevalence of Occult HBV Infection in Adults Vaccinated Against Hepatitis B at Birth.

Background/objectives: Immunization with the hepatitis B vaccine is the most effective means of preventing acute HBV infection. However, whether the primary vaccination of infants confers lifelong immunity remains controversial. Therefore, the ongoing surveillance of vaccine recipients is required.

Visual electrophysiological assessment of children with poor response to treatment for functional amblyopia.

Aim: To assess the visual electrophysiological outcomes in children with functional amblyopia who exhibited poor response to conventional treatment.

Methods: Twenty-one children with functional amblyopia, aged 5.7±2.

Molecular and physiological response of chives (Allium schoenoprasum) under different concentrations of selenium application by transcriptomic, metabolomic, and physiological approaches.

Selenium (Se) is a vital trace element for human health, and its uneven distribution in soil triggers Se deficiencies in some regions. Se biofortification has been demonstrated to mitigate this issue by producing Se-enriched crops. Chives (Allium schoenoprasum cv.

[Molecular mechanism of Xinyang Tablets in improving myocardial fibrosis in uremic cardiomyopathy based on single-cell sequencing technology].

This study aimed to investigate the ameliorative effect of Xinyang Tablets on myocardial fibrosis in uremic cardiomyopathy(UCM) using single-cell sequencing technology. UCM mouse models were established by 5/6 nephrectomy(NPM) and randomly divided into the model group, Xinyang Tablets group, and sham-operated(sham) group as the control. The Xinyang Tablets group received postoperative interventions of Xinyang Tablets(0.

The biomechanical effects of treating double-segment lumbar degenerative diseases with unilateral fixation through interlaminar fenestration interbody fusion surgery: a three-dimensional finite element study.

Background: Transforaminal lumbar interbody fusion (TLIF) surgery has become increasingly popular in the surgical treatment of lumbar degenerative diseases. The optimal structure for stable double-segment fixation remains unclear.

Objective: To compare the biomechanical changes of unilateral fixation versus bilateral fixation in patients with lumbar degeneration undergoing double-segment TLIF surgery, and to explore the stability and feasibility of unilateral double-segment fixation.

Retinal Müller Cell-Released Exosomal MiR-92a-3p Delivers Interleukin-17A Signal by Targeting Notch-1 to Promote Diabetic Retinopathy.

Purpose: Inflammatory processes have been involved in diabetic retinopathy (DR). Interleukin (IL)-17A, a pro-inflammatory cytokine, is associated with DR occurrence and development. However, mechanisms underlying the IL-17A impact on DR need further investigations.