Microbiota-mediated metabolic alterations reveal bidirectional regulation of the gut-nose axis in mice with allergic rhinitis.

Background: Microbes and their metabolites are implicated in respiratory diseases, including allergic rhinitis (AR); however, the interaction between the gut and respiratory tract and the role of microbes remains unclear. We investigated the gut and nasal microbiota variations between AR and control mice and their role in the bidirectional regulation of the gut-nasal axis.

Methods: We validated the OVA-induced establishment of an AR mouse model based on nasal symptoms and histopathology.

Strengthening benefits of panthenol for hair: Mechanistic evidence from advanced spectroscopic techniques.

Objective: Small molecules, like panthenol have been shown to penetrate hair. However, their interactions with the protein structures of hair have not been well studied. In this study, the penetration of panthenol into protein structures of hair was confirmed, and NMR methods were utilized to understand the protein-panthenol interaction.

Incidence and risk factors of intraoperative acquired pressure injury in open heart surgical patients: a meta-analysis of prospective studies.

Purpose: This meta-analysis aimed to evaluate the incidence and risk factors of intraoperative acquired pressure injuries (IAPIs) in open heart surgical patients, focusing exclusively on prospective studies to address gaps in the existing literature.

Methods: A systematic search was conducted across PubMed, Embase, and Web of Science up to January 2025. Data on incidence and risk factors were extracted, and statistical analyses were performed using random-effects models.

A leveling-shielding dual strategy enabling stable zinc anodes to exhibit ultra-low interfacial impedance.

Aqueous zinc-ion batteries have demonstrated great potential for large-scale energy storage, but still suffer from severe dendrites and corrosion problems. Uneven distributions of crystalline planes and passivation layers on the zinc anode are the key factors causing dendrites and corrosion. However, the synergistic construction of uniform crystal planes and shielding layers is still challenging.

Size-segregated emission characteristics and associated toxicity of polycyclic aromatic compounds from agricultural machinery.

Non-road agricultural machinery (AM) emissions now exceed road vehicles as major atmospheric particulate contributors in China. Though recent changes to fuels and emission standards have been implemented, their effects on particle size-resolved chemical compositions and public health impacts remain poorly understood. This study systematically analyzed the particle size-resolved polycyclic aromatic compounds (PACs) chemical composition from 5 AM, evaluating the impacts of fuel type (diesel B0, biodiesel B5/B20), emission standards (stage II/III), and working status (idling, moving, working) on emission characteristics and health risks.

Rhoifolin Attenuates DSS-Induced Colitis in Mice by Modulating Gut Microbiota and Restoring Th17/Treg Balance.

Introduction: Rhoifolin (ROF), a flavonoid compound isolated from citrus plants, has been shown in modern research to possess a range of important biological activities, including anti-inflammatory and anti-tumor properties.

Methods: In this study, we induced ulcerative colitis (UC) in mice using dextran sulfate sodium (DSS) and treated them with ROF during the therapeutic phase.

Results: The results showed that ROF significantly alleviated the weight loss, colon shortening, and histopathological damage in the colon tissues of the mice, while also improving intestinal barrier function by restoring the expression of ZO-1 and E-cadherin.

AI-based diagnosis of acute aortic syndrome from noncontrast CT.

The accurate and timely diagnosis of acute aortic syndrome (AAS) in patients presenting with acute chest pain remains a clinical challenge. Aortic computed tomography (CT) angiography is the imaging protocol of choice in patients with suspected AAS. However, due to economic and workflow constraints in China, the majority of suspected patients initially undergo noncontrast CT as the initial imaging testing, and CT angiography is reserved for those at higher risk.

Discovery of blood-brain barrier transport-related surface proteins by DIA-Orbitrap high-resolution mass spectrometry.

As a selective permeability interface, the blood-brain barrier (BBB) restricts drug access to the brain, complicating theranostic strategies for neurological disorders. The selective permeability of the BBB is primarily controlled by brain microvascular endothelial cells (BMECs). Cell surface proteins can be endocytosed into brain microvascular endothelial cells, serving as promising candidates for receptor-mediated transcytosis across the BBB.

Cultivation and sequencing of microbiota members unveil the functional potential of yak gut microbiota.

The animal gut microbiota exhibits extensive taxonomic diversity, yet cultivated isolates and complete genomes from animal hosts remain scarce, hindering functional and ecological insights. We present a cultivated Yak () Fecal bacteria genome Reference (YFR), comprising 548 high-quality genomes based on aerobic and anaerobic cultivation. Notably, 216 strains represented novel taxa, classified into 29 species-level clusters spanning 4 phyla and 14 genera.

Axial enhancement on non-radicals generation in PMS activation process mediated with Co single-atom catalyst encapsulated nanoparticles.

While numerous studies on catalysts with varying metal site sizes in Fenton-like systems have explored synergistic mechanisms between single atoms (SAs) and nanoparticles (NPs), the axial enhancement mechanism of NPs toward SAs remains underexplored. Here, we revealed the mechanism by which nitrogen-doped graphitic carbon encapsulated NPs axially enhance the activation of surface SAs over peroxymonosulfate (PMS). A novel Co@N-C catalyst coexisting with Co SAs and encapsulated Co NPs was synthesized, in which the internalization of Co NPs accelerated the interfacial mass transfer rate resulting in 4.

Serum β-klotho is a potential biomarker for diagnosing alcoholic liver disease and differentiating from nonalcoholic fatty liver disease.

Background: Alcoholic liver disease (ALD), with the control of infectious liver disease and the improvement in living standards, is emerging as a significant liver disease posing a threat to public health. Besides, ALD often overlaps or coexists with nonalcoholic fatty liver disease (NAFLD), however, due to the lack of specific non-invasive biomarkers and the fact that drinkers' self-reported alcohol consumption is often concealed, the identification of ALD and NAFLD is sometimes not easy. This study aims to explore a new specific serum biomarker to more easily diagnose ALD and differentiate it from NAFLD.

Prediction model developed on the basis of meta-analysis in the field of medicine: a systematic survey and methodological summaries.

Background: We performed a systematic survey to collate studies on prediction models developed on the basis of meta-analysis in medicine and summarize key steps involved in model development.

Methods: We systematically searched Web of Science, PubMed and Embase until April 2023 and included those that developed prediction models on the basis of meta-analysis. The data were summarized via narrative synthesis.

Integrative scATAC-seq and mtDNA mutation analysis reveals disease-driven regulatory aberrations in AML.

Disturbances in transcriptional regulation during hematopoiesis can lead to aberrant hematopoietic differentiation and potential leukemogenesis. Given the high relapse risk of acute myeloid leukemia (AML), understanding its progression mechanisms and identifying prognostic biomarkers are crucial for improving treatment outcomes. Here, we applied a mitochondrial single-cell assay for transposase-accessible chromatin with sequencing mitochondria single-cell ATAC-seq (mtscATAC-seq), supplemented with single-cell RNA-seq, to comprehensively characterize AML cells.

The YY1-ABCB7 regulatory Axis is associated with malignant progression and ferroptosis sensitivity in lung adenocarcinoma.

Aim: Ferroptosis, a newly identified form of regulated cell death, has emerged as a promising therapeutic target in cancer treatment. The study was to clarify the regulatory effects of Yin Yang 1 (YY1) on ATP Binding Cassette Subfamily B Member 7 (ABCB7) expression and its related functions in lung adenocarcinoma (LUAD), focusing on ferroptosis.

Methods: The bioinformatics analysis of ABCB7 expression in LUAD was initially conducted using public databases and subsequently validated through the examination of LUAD tissue samples.

HIV-1 Vpu interacts with RBM10 to promote HIV-1 infection.

Viral protein U (Vpu), an accessory protein of HIV-1, functions by antagonizing or hijacking various host factors to allow the virus to evade host immune surveillance and facilitate viral release. Nevertheless, there is limited understanding regarding the impact of Vpu on the biogenesis of HIV-1 RNAs. In this study, we utilized ascorbate peroxidase 2 (APEX2)-based proximity labeling techniques in combination with mass spectrometry and immunoprecipitation-mass spectrometry (IP-MS) to characterize interactions between HIV-1 Vpu and host proteins.

Bavachinin alleviates allergic rhinitis by modulating gut microbiota and inhibiting NLRP3-mediated epithelial pyroptosis through PI3K/AKT/NF-κB signaling pathway.

Allergic rhinitis (AR), a chronic, non-infectious nasal mucosal inflammation, for which there is no drug that can completely cure it. Bavachinin (BVC), a natural bioactive flavanone from Psoraleae Fructus, has anti-inflammatory and anti-asthmatic properties. However, its effects on AR and the associated mechanism remain unclear.

Ultrasound Improves Gallbladder Contraction Function: A Non-Invasive Experimental Validation Using Small Animals.

Background: Gallbladder hypomotility is a key pathogenic factor in cholelithiasis. Non-invasive interventions to enhance gallbladder contractility remain limited. Ultrasound therapy has shown promise in various muscular disorders, but its effects on gallbladder function are unexplored.

Mechanistic Insights into the Anti-Hepatocellular Carcinoma Effects of ACY-1215: p53 Acetylation and Ubiquitination Regulation.

As a major global health challenge, hepatocellular carcinoma (HCC) still faces substantial limitations in its treatment options. This study investigates the anti-HCC potential of ACY-1215, a selective Histone deacetylase 6 (HDAC6) inhibitor, and its mechanism targeting p53 regulation. In vitro studies conducted with HepG2 and SMMC-7721 cells revealed that ACY-1215 markedly inhibited HCC cell proliferation, migratory capacity, and invasive potential, as evidenced by CCK-8, colony formation, and Transwell assays.

A 3D Printed Meridian-Arrayed Microfluidic Device for Dual-Physical Fields Induced Highly Efficient Intracellular Delivery.

Intracellular delivery is a critical process in various biological studies and applications, encompassing genomic manipulation, biomanufacturing, and cell-based therapeutics. Traditional macro-scale delivery approaches have been hindered by cumbersome and lengthy processes, resulting in low cell viability and limited scalability. Microfluidic and nanoengineering-based platforms have shown promise due to their scale compatibility with individual cells.

Tangeretin protects the kidney from ischemia-reperfusion injury by inhibiting inflammatory response through downregulation of MAOA.

Objective: This study investigates the protective effects and mechanisms of the natural antioxidant tangeretin in alleviating renal ischemia-reperfusion injury (RIRI), a major cause of acute kidney injury (AKI).

Methods: Firstly, a RIRI mouse model was established to investigate the renoprotective effects of tangeretin. Potential therapeutic targets were identified through RNA sequencing and network pharmacology analysis and subsequently validated using the GEO and KIT databases.

Genotypic and phenotypic characteristics of group B streptococcal isolates from neonates in a tertiary hospital in Qingyang, Gansu Province, China.

Group B Streptococcus (GBS) causes severe neonatal infections, leading to high mortality rates. This study aimed to investigate the genetic characteristics of GBS isolates in a hospital setting. Nineteen isolates were identified through a review of preservation and cultivation processes since January 2022, followed by antibiotic susceptibility testing.

TBK1 phagosomal recruitment enhances antifungal immunity via positive feedback regulation with SRC.

TANK-binding kinase 1 (TBK1) is a versatile serine/threonine protein kinase that is mainly recognized for its canonical role in antiviral immunity through interferon induction. Here, we report a previously uncharacterized function for TBK1 in antifungal defense. Using proteomic analysis, we identified TBK1 as a phagosome-directed protein after fungal infection, and biochemical analysis revealed that TBK1 is directly recruited to the phagosome by SHP2 in a process driven by SRC-mediated phosphorylation.

Synergistic inhibition of pathogenic fungi by oleanolic acid combined with azoles.

Invasive fungal infections and emerging antifungal resistance mediated by pathogenic fungi, notably spp., spp., , and pose a critical challenge to global public health systems.

Selective lithium extraction and Fe-P component reconstruction toward high-performance NaFe(PO)PO as cathode materials of sodium ion batteries.

NaSO oxidation reclaims 98.08% Li from spent LiFePO, while residual Fe-P components are reconstituted into NaFe(PO)PO/C Na-insertion and carbon coating. The regenerated cathode delivers 99.