A 3D-printed cassette coupled with a paper-based aptasensor for advanced diagnosis of chikungunya virus towards commercialization.

Electrochemical sensors have attracted significant attention because of their vital role in the early identification of infectious diseases, indicating their usefulness in various diagnostic applications. Here, the present study reports the development of paper-based electrochemical aptasensors integrated into a 3D printed cassette for chikungunya virus detection at the point of care. The sensor is functionalised with a highly specific aptamer and employs silver nanoparticles to enhance electrochemical sensitivity.

Pre-analytical interference in point-of-care troponin T testing: a case series.

Objectives: To examine the impact of haematological and biochemical abnormalities on the failure of point-of-care troponin T (POCT) testing in patients with suspected acute coronary syndrome (ACS).

Case Presentation: Five patients underwent Roche Troponin T Card Test (lateral flow immunoassay) in an emergency setting. Despite correct sampling and procedural adherence, no valid results were obtained resulting in an aborted test.

Deciphering morpho-physiochemical diversity, mineral profiling, and population structure in brinjal ( L.) germplasm.

Unlabelled: Brinjal ( L.) is a key solanaceous vegetable in Asian and African countries, rich in minerals and vitamins in tropical diets. This study evaluated the variability and genetic diversity of 94 genotypes grown in an Augmented Randomized Complete Block Design during (monsoon-winter) 2023-24.

Targeting cancer-associated fibroblast-driven LIF/LIFR axis improves the therapeutic efficacy of gemcitabine and nab-paclitaxel in pancreatic cancer.

Pancreatic ductal adenocarcinoma (PDAC) is inherently therapy resistant due to cancer cell-stroma crosstalk across several signaling pathways. Among these, the LIF/LIFR axis has been implicated in cancer cell and cancer-associated fibroblast (CAF) crosstalk. We evaluated the efficacy of EC359, a competitive inhibitor of LIFR, in combination with gemcitabine.

Study on Thermal Damage in 3D Tumor During Magnetic Nanoparticle Hyperthermia Using Space-Time Fractional Dual Phase Lag Bioheat Model.

Magnetic nanoparticles for hyperthermic cancer treatment have gained significant attention in recent years. Magnetic hyperthermia ablates malignant cells by dissipating heat from magnetic nanoparticles (MNPs) when subjected to an alternate magnetic field. Living tissues are highly non-homogeneous, and non-Fourier thermal behavior in biological tissue has been experimentally observed.

Radiofrequency Electromagnetic Field Emissions and Neurodevelopmental Outcomes in Infants: A Prospective Cohort Study.

Purpose It has been argued that children are particularly at risk of developing health effects due to the emitted radiofrequency electromagnetic fields (RF-EMF). We designed this cohort to measure the association between exposure to RF-EMF radiation and neurodevelopmental changes in neonates and infants. Methods We present an analysis of 261 observations from a cohort of 105 neonates.

Prediction of speed of sound of deep eutectic solvents using artificial neural network coupled with group contribution approach.

Predicting the physiochemical properties of deep eutectic solvents (DESs) is crucial for designing new solvents. Heat capacity and speed of sound are important thermodynamic properties in chemical processes. However, experimental data on the speed of sound in DESs is limited.

Prevalence of Iron Deficiency and Iron Deficiency Anemia Among Adolescent Girls at a Tertiary Care Centre in Bihar: A Cross-Sectional Study.

Background Iron deficiency (ID) and iron deficiency anemia (IDA) are significant public health concerns, particularly in low- and middle-income countries. Adolescents, especially girls, are at a heightened risk due to increased iron requirements during growth and menstruation. Objective This study aimed to estimate the hospital prevalence of ID and IDA among adolescent girls attending the Outpatient Department (OPD) at a tertiary care centre in Bihar, India.

Surface Engineered 2D-β-ketoenamine Covalent Organic Framework for Superior Dechlorination via Hybrid Capacitive Deionization.

To prevent a global water shortage crisis, we must innovate to desalinate seawater. Hybrid capacitive deionization (HCDI), an energy-efficient desalination method, depends on electrode materials. Removing chloride ions (Cl), a key salinity factor, is vital for balanced electrode capacity.

Enhanced selective gold recovery from e-waste synergistic hetero-atom controlled quasi-planar benzoxazine-based covalent organic frameworks.

Efficient and selective gold recycling from e-waste mixtures is crucial for advancing sustainability, the circular economy, and waste management objectives. However, the complex composition of e-waste and the underdeveloped practical potential of current adsorbents highlight the need for innovative sorbent development. Here, we present quasi-planar, benzoxazine-core-based two-dimensional covalent organic frameworks (COFs) with synergistic hetero-atom control across the network, achieved a multi-component synthetic approach.

Synthesis, Computational Studies of New Chalcone Derivatives as Anxiolytics and Skeletal Muscle Relaxants.

Introduction: Chalcone derivatives are known for their diverse biological activities, including anxiolytic and skeletal muscle relaxant properties. Recent studies indicate that structural modifications can enhance their therapeutic effectiveness. This study aimed to synthesize and biologically evaluate novel chalcone derivatives, investigating their structure-activity relationship through computational studies and assessing their pharmacological potential.

Unlocking the Power of CXCR2 Inhibition to Overcome Gemcitabine Resistance in Pancreatic Cancer.

Pancreatic ductal adenocarcinoma (PDAC) is the fourth leading cause of cancer-related mortality, characterized by intrinsic resistance to conventional therapies and limited effective treatment options. In this study, we investigated the role of the CXCR2 axis in PDAC therapy resistance. CXCR2, a chemokine receptor, is actively involved in inflammation, tumor angiogenesis, and metastasis.

Effectiveness of Oral Nifedipine Versus Intravenous Labetalol in Controlling Hypertension in Severe Preeclampsia: A Comparative Study.

Background: This study aimed to study and compare the efficacy and side effects between oral nifedipine and intravenous labetalol in controlling hypertension in acute cases of severe preeclampsia.  Methods: A prospective comparative study of pregnant patients with blood pressure >160/110 mm Hg after 20 weeks of gestation was conducted in the Department of Obstetrics and Gynecology of MGM Medical College and Hospital, Navi Mumbai, India, over a period of one and a half years from August 1, 2022, to December 31, 2023. A total of 100 cases were studied, 50 receiving oral nifedipine and the other 50 receiving intravenous labetalol.

Synthesis and evaluation of lactam and maleimide derivatives of 16-hydroxycleroda-3, 13 (14) Z-dien-15, 16-olide from Polyalthia longifolia as potential anticancer agents.

Diterpenes are plant secondary metabolites with diverse bioactivities, yet their anticancer potential remains underexplored. Here, we report the design and synthesis of thirty-nine novel derivatives of clerodane diterpene 1, isolated from Polyalthia longifolia, including twenty lactam and nineteen maleimide analogs. All compounds were screened at 10 μM against colon (DLD-1, HCT116, HT-29) and breast (MCF-7, MDA-MB-231, 4T1) cancer cell lines.

Maternal obesity programs cardiac remodeling in offspring via epigenetic, metabolic, and immune dysregulations.

Maternal obesity during pregnancy significantly increases the offspring's risk of later-life cardiovascular disease. This study investigated cardiometabolic perturbations by utilizing a mouse model of maternal high-fat diet (HFD)-induced obesity that recapitulates metabolic abnormalities observed in humans. We report that offspring of HFD-fed mothers (Off-HFD) exhibit a progression of obesity, hypertension, dyslipidemia, and metabolic inflexibility when compared with offspring of regular diet-fed mothers (Off-RD).

MOF-derived Fe-doped δ-MnO nanoflowers as oxidase mimics: chromogenic sensing of Hg(II) and hydroquinone in aqueous media.

The structure and morphology play a crucial role in enhancing the biomimetic oxidase activity of nanozymes. In this study, a facile chemical oxidation strategy was employed to synthesize MOF-derived MnO, utilizing the structural features of the parent MOF to enhance oxidase-mimicking activity. We have systematically investigated the effects of phase evolution, structural modulation and morphology on the oxidase activity of MnO with Fe substitution.

Identification of QTLs associated with modifiers influencing kernel opacity, kernel hardness, and tryptophan content in quality protein maize.

Lysine and tryptophan, two essential amino acids, are generally deficient in normal maize but enriched in () mutants. However, these mutants are linked to undesirable effects like soft endosperm and yield loss. To circumvent this, researchers introgressed (s) into mutant maize and developed Quality Protein Maize (QPM).

Effect of Distal Fragment Length on Construct Stability in an Extra-articular Distal Tibial Fracture Model Fixed With Locked Intramedullary Nailing: A Biomechanical Study.

Introduction  Fractures of the distal tibia are complex injuries with high complication rates, which include delayed union, non-union, and wound complications like dehiscence and infection. The two commonly employed definite internal fixation modalities include locked intramedullary (IM) nailing and plating. There is controversy regarding the superiority of the fixation construct, although nailing is proven to be more biological and devoid of soft tissue complications.

Determination of progoitrin, sinigrin, and glucoerucin contents and the expression pattern of aliphatic glucosinolates biosynthesis pathway genes in different maturity groups of cauliflower.

Unlabelled: The present study analyzed glucosinolates in ten commercial varieties/hybrid from all four maturity groups (i.e., Early, Mid-early, Mid-late, and Late) of cauliflower using Ultra-Performance Liquid Chromatography (UPLC) linked to a Quadrupole-Time of Flight Mass Spectrometer.

Functional analysis of the gene in for enhancing tolerance to .

Alternaria blight (Alternaria burnsii ) causes significant economic losses due to defoliation, reduced yields, and poor-quality produce in various crops. Consequently, effective strategies for managing this disease are critical. In this study, the caffeoyl-CoA O-methyltransferase (PdCCoAOMT ) gene, which plays a key role in lignin biosynthesis and plant defense, was isolated from Populus deltoides and investigated for its potential to enhance resistance against A.

Co-relation of coagulation profile with glycemic index in patients with type 2 diabetes.

Background: Diabetes mellitus (DM) has emerged as a significant global concern that accounts for nearly 90% of type 2 DM cases worldwide and characterized by insulin resistance. Hyperglycemia in diabetes leads to increased fibrinogen levels and activates the coagulation cascade, thereby triggering atherothrombotic events.

Aim And Objective: The study was designed to evaluate the coagulation profile (activated partial thromboplastin time, prothrombin time, and INR) in type 2 diabetes and to analyze the correlation between glycated hemoglobin (A1C) and coagulation profile among the OPD patients coming to AIIMS Patna.

Designing of moringa gum-zwitterionic copolymer structure through supra-molecular and covalent interactions for biomedical uses.

Recently, functional materials derived from carbohydrate polymers have gained significant attention for their clinical uses due to their inherent bioactivity and biocompatibility. Therefore, the primary focus of the present research was to design bioactive moringa gum (MOGUM)-based hydrogels through covalent and supra-molecular interactions for use in biomedical applications. The copolymeric hydrogels were prepared by crosslinking of zwitterionic polymers of 2-(methacryloyloxy)ethyl] dimethyl-(3-sulfoproyl) ammonium hydroxide (MEDSAH) and carbopol (CP) onto gum for their applications in hydrogel wound dressings (HWDR) and drug delivery (DD).

The Mi- 1 gene is a key regulator of defence mechanisms and cellular gene dynamics in response to root-knot nematodes.

Root-knot nematodes threaten tomato cultivation worldwide. This study confirms Mi- 1 gene's role in defence by inducing programmed cell death, lignin accumulation, and cellular remodelling in resistant plants. Root-knot nematodes, particularly Meloidogyne species, pose a significant economic threat to tomato cultivation globally.

Meta-analysis of QTLs for drought, heat, and salinity tolerance identifies high-confidence MQTLs and candidate genes in chickpea ( L.).

A meta-analysis of 712 quantitative trait loci (QTLs) associated with traits conferring tolerance to drought, heat, and salinity stress was conducted in chickpea. Of these, 588 QTLs were successfully projected onto a high-density consensus map saturated with 14,246 markers. The resulting meta-QTLs (MQTLs) (54) had an average confidence interval (CI) of 1.

Surface N- or O-linked glycans on bovine spermatozoa play minimal role in evading macrophage mediated phagocytosis.

Bull spermatozoa possess glycocalyx made of carbohydrate moieties attached to proteins and lipids on their membranes that is involved in fertility associated functions including immune evasion in the female reproductive tract. The current study aimed to establish whether the differences in the glycocalyx of spermatozoa provide selective advantage in evading phagocytosis mediated by female macrophages. Based on removal of either N- or O-linked surface glycans from the spermatozoa, their susceptibility to phagocytosis by macrophages was assessed in bovines () through flow cytometry.