Transmission electron microscopy reveals the impact of Or gene on chromoplast morphology and β-carotene accumulation in Or gene introgressed tropical cauliflower.

Cauliflower Or gene governs massive accumulation of β-carotene in the edible 'curd' portion. However, homozygous dominant (OrOr) plants accumulate higher β-carotene than heterozygous (Oror) individuals, yet this phenomenon has not been thoroughly elucidated in relation to chromoplast morphology. A study was performed in a complete randomised block design with three replicates, in which curd samples of homozygous orange (OrOr; CF) heterozygous orange (Oror; CF) and white (oror; CF) genotypes were analysed using transmission electron microscopy (TEM).

Understanding the changes in endogenous GA3 in relation to developmental transitions in cauliflower (Brassica oleracea var. botrytis L.).

Cauliflower is a crop with intricate developmental transitions influenced by both external and internal cues. Plant growth regulators (PGRs) play a key role in developmental transitions and plant responses to environmental factors. The present study aimed to investigate the endogenous levels of gibberellins (GAs) (i) at six different developmental stages and (ii) at four time points in four varieties of cauliflower using high-performance liquid chromatography (HPLC).

Effect of a Triad of Norepinephrine, Tranexamic Acid, and Endo-Cutter Staplers on the Reduction of Blood Loss and Operative Duration in Patients Undergoing Open Radical Cystectomy.

Objective: To evaluate the effect of norepinephrine, tranexamic acid, and endo-cutter staplers on the reduction of blood loss and operative duration in patients undergoing open radical cystectomy (ORC).

Methods: We conducted a prospective case-control study involving 50 patients who underwent ORC. The first group consisted of 25 patients (cases) who received norepinephrine and tranexamic acid-soaked mops, along with endo-cutter staplers for ligating the vascular pedicles during the surgery.

Lu-PSMA-617 Consolidation Therapy After Docetaxel in Patients with Synchronous High-Volume Metastatic Hormone-Sensitive Prostate Cancer: A Randomized, Phase 2 Trial.

Lu-prostate-specific membrane antigen-617 (Lu-PSMA-617) has shown positive survival outcomes in metastatic castration-resistant prostate cancer. However, there are limited data in the hormone-sensitive setting. Here, in the CONSOLIDATE trial (Lu-PSMA-617 Consolidation Therapy After Docetaxel in Patients with Synchronous High-Volume Metastatic Hormone-Sensitive Prostate Cancer), we intended to evaluate the role of Lu-PSMA-617 as consolidation therapy for residual disease after chemohormonal treatment in patients with synchronous high-volume metastatic hormone-sensitive prostate cancer (mHSPC).

Determination of progoitrin, sinigrin, and glucoerucin contents and the expression pattern of aliphatic glucosinolates biosynthesis pathway genes in different maturity groups of cauliflower.

Unlabelled: The present study analyzed glucosinolates in ten commercial varieties/hybrid from all four maturity groups (i.e., Early, Mid-early, Mid-late, and Late) of cauliflower using Ultra-Performance Liquid Chromatography (UPLC) linked to a Quadrupole-Time of Flight Mass Spectrometer.

Utility of PSMA-PET derived volumetric parameters in initial risk stratification and prediction of prostate cancer metastasis - a head-to-head comparison of the radiotracers 18 F-PSMA-1007 and 68 Ga-PSMA-11.

Objective: This study aimed to explore and compare the utility of baseline 18 F-PSMA-1007 and 68 Ga-PSMA-11 PET/computed tomography (CT) derived volumetric parameters in initial risk stratification and prediction of prostate cancer (PCa) metastasis.

Methods: Forty treatment-naïve, biopsy-proven intermediate-/high-risk PCa patients were prospectively recruited. Each patient underwent PET/CT with 68 Ga-PSMA-11 and 18 F-PSMA-1007 (within 2 weeks).

Prognostic significance of Klotho expression in patients with renal cell carcinoma.

Introduction: Various molecular markers have been investigated in renal cell carcinoma (RCC) without significant reliability. We analyzed Klotho (tumor suppressive protein) expression in RCC to investigate its association with tumor-stage, grade, disease-free-survival (DFS) and overall-survival (OS).

Methods: Data of histologically confirmed patients of RCC with complete clinical follow-up were retrieved from Medical-Record-Library.

Commercial suitability and characterization of newly developed Erucastrum canariense (Can) sterile cytoplasm based cytoplasmic male sterile (CMS) lines in Indian cauliflower.

The study presents the first to characterize novel Erucastrum canarianse Webb and Berthel (or Can) sterile cytoplasm-based CMS lines in Indian cauliflower (Brassica oleracea var. botrytis L.) and investigating their commercial suitability.

[Lu]Lu-PSMA-617 Versus Docetaxel in Chemotherapy-Naïve Metastatic Castration-Resistant Prostate Cancer: Final Survival Analysis of a Phase 2 Randomized, Controlled Trial.

The prostate-specific membrane antigen (PSMA) inhibitor [Lu]Lu-PSMA-617 has been previously demonstrated to be noninferior to docetaxel in achieving a biochemical response in chemotherapy-naïve metastatic castration-resistant prostate cancer patients. Here, we report the final analysis of overall survival (OS) for a phase 2 randomized, controlled trial. Forty chemotherapy-naïve, PSMA-positive metastatic castration-resistant prostate cancer patients were randomly assigned to [Lu]Lu-PSMA-617 ( = 20) or docetaxel ( = 20).

Assessment of data intelligence algorithms in modeling daily reference evapotranspiration under input data limitation scenarios in semi-arid climatic condition.

Crop evapotranspiration is essential for planning and designing an efficient irrigation system. The present investigation assessed the capability of four machine learning algorithms, namely, XGBoost linear regression (XGBoost Linear), XGBoost Ensemble Tree, Polynomial Regression (Polynomial Regr), and Isotonic Regression (Isotonic Regr) in modeling daily reference evapotranspiration (ET) at IARI, New Delhi. The models were developed considering full and limited dataset scenarios.

Evaluation of an automated rapid urine culture method for urinary tract infection: Comparison with gold standard conventional culture method.

Purpose: Urinary tract infection is one of the most prevalent disease affecting people from all age groups. For its diagnosis, conventional culture and antibiotic susceptibility is the gold standard. However, its major limitation is that the results take minimum of 24 ​h and antibiotic susceptibility is available after 48 ​h.

Effect of ureteric access sheath on outcomes of retrograde intrarenal surgery for renal stone disease: a randomized controlled trial.

Background: The need of ureteric access sheath (UAS) in retrograde intrarenal surgery (RIRS) has been controversial for gaining high success in terms of stone free rate (SFR), reducing operative times and complications. There has been lack of high level of evidence in the literature on this topic.

Materials And Methods: This prospective randomized controlled trial (RCT) was conducted in the Department of Urology, PGIMER, Chandigarh from July 2019 to Dec 2021.

Treatment outcomes of adjuvant radiotherapy in adrenocortical carcinoma - A 13-years experience from a tertiary care centre.

Purpose: Adrenocortical carcinoma (ACC) is a rare and highly aggressive malignant neoplasm, usually diagnosed in advanced stage. Role and efficacy of adjuvant radiotherapy has not been well defined. The objective of this study is to describe the various clinical characteristics and prognostic factors affecting the survival of ACC along with the role radiotherapy on overall survival and relapse free survival.

Iatrogenic partial glanular amputation: A rare complication of circumcision.

Circumcision is a customary ritual across many cultures. However, the safety of such procedures remains a concern. A boy underwent circumcision in 2014 by a religious worker at the age of 7 years.

Screening of short-day onions for resistance to leaf blight in the seed-to-bulb stage (stage I) and bulb-to-seed stage (stage II).

leaf blight, caused by , is a very important fungal disease in onions since its epidemics are able to affect both the bulb yield and the seed quality. The aim of this study was to screen onion genotypes at stage I (seed to bulb) and further screen the identified resistant and susceptible genotypes at stage II (bulb to seed). One hundred and fifty-seven genotypes were screened against SLB under artificially inoculated field conditions.

Genomic characterization of metastatic castration-resistant prostate cancer patients undergoing PSMA radioligand therapy: A single-center experience.

Background: Genomic defects in DNA-damage repair (DDR) mechanisms have been proposed to affect the radiosensitivity of prostate cancers. In this study, we intended to evaluate the prevalence of genetic alterations in a cohort of metastatic castration-resistant prostate cancer (mCRPC) patients undergoing radioligand therapy (RLT) with prostate-specific membrane antigen (PSMA)-inhibitors as well as the impact of such mutations on treatment outcomes.

Methods: Data of consecutive mCRPC patients from 2017 to 2021 who were treated with PSMA-RLT and underwent next-generation sequencing (NGS) were collected and analyzed for response and survival outcomes.

Comparison of 18 F-PSMA-1007 PET/CT With 68 Ga-PSMA-11 PET/CT for Initial Staging in Intermediate- and High-Risk Prostate Cancer.

Purpose: This study aimed to compare 18 F-PSMA-1007 PET/CT with 68 Ga-PSMA-11 PET/CT for initial staging in intermediate- and high-risk prostate cancer (PCa) patients.

Methods: Forty treatment-naive, biopsy-proven, intermediate- or high-risk PCa patients were prospectively recruited. Each patient underwent PET/CT with 68 Ga-PSMA-11 and 18 F-PSMA-1007 (within 2 weeks).

Robot-assisted partial nephrectomy for moderate to highly complex renal masses. A systematic review and meta-analysis.

Introduction: Multiple studies have been published recently assessing feasibility of robot-assisted partial nephrectomy (RAPN) for moderate to highly complex renal masses. Some studies have even compared partial nephrectomy (PN) performed through various modalities such as open PN (OPN) versus RAPN and laparoscopic PN (LPN) versus OPN. The primary aim of this review was to analyze perioperative outcomes such as warm ischemia time (WIT), duration of surgery, estimated blood loss (EBL), complications, blood transfusion, length of stay, and margin status following RAPN for complex renal masses.

Long-term impact of testicular torsion and its salvage on semen parameters and gonadal function.

Introduction: Testicular torsion is a urological emergency, and long-term outcomes of testicular torsion on infertility, hormonal function, and salvaged testicular size are unclear.

Materials And Methods: We conducted an ambispective, observational study from January 2014 to December 2019. Baseline demographics, time of presentation, clinical features, and management details of all the patients of testicular torsion were recorded from the database.

Current practice patterns in the perioperative management of patients undergoing radical cystectomy: Results from a global survey.

Objective: To determine current practice patterns and adherence to various components of enhanced recovery after surgery in cystectomy (ERAC) protocol for peri-operative management of radical cystectomy patients through a global survey.

Methods: A survey containing 25 questions and addressing 15 of the 22 items of the ERAC protocol was developed and disseminated through Email to the urologists with recent bladder cancer publications. The mailing list was generated by retrieving Email-ids of corresponding authors of articles using the keyword "cystectomy" in Scopus from January 2018 to October 2020.

Safety and Diagnostic Yield of Ga Prostate-specific Membrane Antigen PET/CT-guided Robotic-assisted Transgluteal Prostatic Biopsy.

Background Transrectal US-guided biopsy with or without MRI fusion is performed for diagnosing prostate cancer (PCa) but has limitations. Gallium 68 (Ga) prostate-specific membrane antigen (PSMA) PET/CT-guided targeted biopsy has the potential to improve diagnostic yield of PCa. Purpose To evaluate the safety and diagnostic yield of Ga PSMA PET/CT-guided, robotic-arm assisted transgluteal prostatic biopsy.

68Ga-PSMA-11 uptake in tracheal mucus plug - a potential pitfall.

Prostate-specific membrane antigen (PSMA) PET/CT is a well-acclaimed imaging modality for prostate cancer (PCa). However, many reports have highlighted PSMA radioligand uptake in a variety of benign tumors and lesions. We report a case of PCa in which  Ga-PSMA-11 uptake was noted in the tracheal lumen.