Prognostic Value of a Novel Model for Hepatocellular Carcinoma Patients Undergoing Transarterial Chemoembolization.

Background: Hepatocellular carcinoma (HCC) patients who underwent transarterial chemoembolization (TACE) have heterogeneous clinical outcomes. Accurate prognosis prediction and risk stratification are crucial for individualized treatment. We sought to develop a novel prognostic model for overall survival (OS) that incorporated contemporary clinical and laboratory factors for estimating individual prognosis.

Plant and probiotic-based edible vaccines for Helicobacter pylori: Research status and application prospects.

Over half of the global population suffers from Helicobacter pylori infection, linked to gastrointestinal disorders and even gastric cancer. As antibiotic resistance increases, so do treatment failure rates, creating an urgent demand for more effective treatments. Plant-derived or probiotic-expressed antigenic proteins elicit immunity via oral delivery, offering a cost-effective anti-colonization strategy.

Coated nanoparticles enhance immune efficacy of Helicobacter pylori outer membrane vesicles by activating Th1/Th2/Th17 responses.

Antibiotic resistance has emerged as a pressing global public health crisis. The development of a safe and efficient Helicobacter pylori vaccine has become an important measure to eradicate Hpylori infection. Outer membrane vesicles (OMVs) secreted by Gram-negative bacteria are immunogenic and have received increasing attention in the development of vaccines against bacterial infections.

GC-MS-driven network pharmacology analysis reveals the bacteriostatic potential of Amomum villosum Lour. Essential oil against Helicobacter pylori in vitro.

Amomum villosum Lour. (A. villosum), a renowned traditional Chinese medicine, is extensively utilized in China for treating gastrointestinal diseases.

An immunoassay based on polyHRP signal amplification for HPV16/18 E6 RNA detection with anti-DNA-RNA hybrid antibody.

Human papillomavirus (HPV) types 16 and 18 are major causes of cervical cancer, with their E6 oncogenes serving as key biomarkers for early diagnosis. Traditional molecular detection methods, such as PCR, are commonly used but still have limitations, including false positives and complex procedures. To address these challenges, we developed an amplification-free sandwich direct enzyme-linked immunoassay (sELISA).

Anti-Melanoma Activity and Potential Mechanism of Purified Potato Protease Inhibitor.

Melanoma is one of the most lethal cancers originating from melanocytes. Its incidence and mortality have been rising rapidly for several decades and have posed a serious threat to human health. Current melanoma treatments are hindered by the scope of application, low efficiency, high cost, and toxic side effects.

Inhibition of LPS-induced NO production by plant essential oils.

Essential oils (EOs), derived from medicinal plants, has been reported to possess various bioactivities to treat inflammatory diseases. Therefore, it is urgent to investigate the effect and the mechanism of EOs essential oils as naturally anti-inflammatory agents. The present study indicated thirty-one EOs had different inhibition on lipopolysaccharide (LPS)-induced nitric oxide (NO) production.

Preparation and evaluation of IgY against human papillomavirus.

Human papillomavirus (HPV) infection is a major global health challenge and is closely related to the occurrence of diseases such as cervical cancer. Unfortunately, effective treatments are still lacking. In view of the advantages of antibody drugs, antibody-targeted therapy may become one of the means of treatment and prevention of HPV infection.

Lipidomic profiling of the febrile rat hypothalamus by the intervention of Artemisia japonica extracts.

Artemisia species have been regarded as an important source of ethnic medicinal plants, such as A. annua and A. capillaris, both of which are widely used in clinical treatment.

Synthesis and Biological Evaluation of Naproxen Derivatives as Novel NLRP3 Inhibitors.

Naproxen, widely used to treat anti-inflammatory diseases, would cause serious of side effects. Based on the biological activities of cinnamic acid, naproxen derivatives containing cinnamic acid were designed, synthesized and used to enhance their anti-inflammatory activities and safeties. The results investigated that thirty novel naproxen derivatives had inhibitory effects on the nitric oxide (NO) release in RAW264.

Discovery of Cinnamic Acid Derivatives as Potent Anti- Agents.

Antibiotics are currently used for the treatment of (), which is confirmed to be the major cause of gastric disorders. However, the long-term consumption of antibiotics has already caused antibiotic resistance and side effects in vivo. Therefore, there is an emerging need for searching for safe and effective anti- agents.

Exploring of exosomes in pathogenesis, diagnosis and therapeutic of osteonecrosis of the femoral head: the mechanisms and signaling pathways.

Osteonecrosis of the femoral head (ONFH) is a refractory disease affecting young adults, resulting in severe hip pain, femoral head collapse, and disabling dysfunction. By far, the underlying mechanism of its pathology is unclear, and still lack of a mature and effective treatment. Exosomes, a regulator of cell-cell communication, their cargos may vary in response to different physiological or pathological conditions.

Bioactive Phenylpropanoid Glycosides, Dimers, and Heterodimers from the Bark of (L.) J.Presl.

Six new phenylpropanoid glycosides (-), two new phenylethanol glycosides ( and ), one new phenylmethanol glycoside (), three new phenylpropanoid dimers (-), two new phenylpropanoid-flavan-3-ol heterodimers ( and ), and six known relevant compounds (-) were isolated and identified from the well-liked edible and medicinal substance (the bark of (L.) J.Presl).

Dual lateral flow assay based on PdRu nanocages for human Papillomavirus detection.

Cervical cancer is one of the most common gynecological malignancies, with the vast majority of which being caused by persistent infection with Human Papillomavirus (HPV) 16 and 18. The current available HPV detection methods are sensitive and genotyped but are restricted by expensive instruments and skilled personnel. The development of an easy-to-use, rapid, and cost-friendly analysis method for HPV is of great need.

peptides alleviated alcoholic liver injury the gut-liver axis.

Alcoholic liver injury has become a leading threat to human health, with complicated pathogenesis and limited therapeutic options. Our previous study showed that peptides (MSPs) exhibit protective potential against early-stage alcoholic liver injury, although the underlying mechanism is not yet clear. In this study, histopathological analysis, mRNA abundance of injury-associated biomarkers, the gut microbiota, and faecal metabolome were evaluated using a mouse model subjected to acute alcohol exposure, aiming to identify the mechanism by which MSP can alleviate alcoholic hepatotoxicity.

Hollow versatile Ag@Pt alloy nanoparticles with nanozyme activity for detection and photothermal sterilization of Helicobacter pylori.

In view of a large number of people infected with Helicobacter pylori (H. pylori) with great harm followed, there is an urgent need to develop a non-invasive, easy-to-operate, and rapid detection method, and to identify effective sterilization strategies. In this study, highly specific nanoprobes with nanozyme activity, Ag@Pt nanoparticles (NPs) with the antibody, were utilized as a novel lateral flow immunoassay (LFIA).

Rapid immunoassay for dual-mode detection of HPV16 and HPV18 DNA based on Au@PdPt nanoparticles.

Cervical cancer (CC) remains one of the most severe global health challenges affecting women, primarily due to persistent infection with high-risk human papillomavirus (HPV) subtypes, particularly with HPV16 and HPV 18. Effective detection of these high-risk HPV strains is crucial for CC prevention. Current screening programs for HPV DNA include PCR and hybridization, which are accurate and sensitive.

GAA/(Au-Au/IrO)@Cu(PABA) reactor with cascade catalytic activity for α-glucosidase inhibitor screening.

Background: In vitro screening strategies based on the inhibition of α-glucosidase (GAA) activity have been widely used for the discovery of potential antidiabetic drugs, but they still face some challenges, such as poor enzyme stability, non-reusability and narrow range of applicability. To overcome these limitations, an in vitro screening method based on GAA@GOx@Cu-MOF reactor was developed in our previous study. However, the method was still not satisfactory enough in terms of construction cost, pH stability, organic solvent resistance and reusability.

An AgPd NP-based lateral flow immunoassay for simultaneous detection of glycocholic acid and alpha-fetoprotein.

Hepatocellular carcinoma (HCC) remains a leading cause of cancer-related mortality globally, ranking third in cancer deaths. Early diagnosis of HCC markers is imperative for effective prognosis and treatment. This study explores the utility of glycocholic acid (GCA) and alpha-fetoprotein (AFP) as biomarkers for liver diseases, with a specific focus on their simultaneous detection for enhanced diagnostic and prognostic capabilities.

Quantification of soluble epoxide hydrolase inhibitors in experimental and clinical samples using the nanobody-based ELISA.

To ensure proper dosage of a drug, analytical quantification of it in biofluid is necessary. Liquid chromatography mass spectrometry (LC-MS) is the conventional method of choice as it permits accurate identification and quantification. However, it requires expensive instrumentation and is not appropriate for bedside use.

The Generation of a Nanobody-Based ELISA for Human Microsomal Epoxide Hydrolase.

A microsomal epoxide hydrolase (mEH) metabolizes in vivo in both xenobiotic and endogenous epoxides associated with signaling function. Findings in patients suggest that mEH might be a biomarker for several diseases, including metastatic cancer and viral hepatitis. To easily quantify mEH, nanobodies specific to the human mEH were isolated from a phage library of llama VHHs.

Lateral flow immunoassay (LFIA) for dengue diagnosis: Recent progress and prospect.

Dengue is one of the most widespread and fatal arboviral infections in the world. Early detection of dengue virus (DENV) is essential to prevent the spread of the disease and provide an immediate response. The lateral flow immunoassay (LFIA) systems are low-cost, rapid, sensitive, targeted, and straightforward detection, which is an ideal early detection candidate for point-of-care testing (POCT) in dengue-affected areas.